LOCUS       pEP0004_pIVTRup(BsmBI)_AncBE4max_        9768 bp    DNA     circular UNA 01-JUL-2019
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...3k-br7smtm
VERSION     urn.local...3k-br7smtm
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     misc_feature    1..4155
                     /Source="referenceToDocument: urn:local:.:sg-br7sn3s"
                     /label="PCR_01_Backbone Copy for Gibson  (concatenated
                     sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    0^1
                     /Original_Bases="ATCGCTGCCTTCTGCGGGGCATCGCTGCCTTCTGCGGGGCT
                     TGCCT"
                     /label="ATCGCTGCCTTCTGCGGGGCATCGCTGCCTTCTGCGGGGCTTGCCT"
                     /note="Geneious type: Editing History Deletion"
     cds             <1..81
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="101362 - pIVTRup Copy; dCas9-VP64"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="101362 - pIVTRup Copy, 101362 - pIVTRup
                     PCR DraI, Digestion of 101362 - pIVTRup PCR DraI -
                     Fragment 3, dCas9-VP64"
                     /Transferred_Similarity="100.00%"
                     /label="5'UTR"
     primer_bind     complement(59..81)
                     /created_by="primer3"
                     /annotation_group="46977673246888b71f4f9-b1a0-4d72-9e0d-72
                     79e21f23e1"
                     /%GC="52.2"
                     /Tm="58.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTAGACCCTCACTTCCTACTCAG"
                     /Transferred_From="pIVTRup_BsmBI_insert_REV"
                     /Transferred_Similarity="100.00%"
                     /label="pIVTRup_BsmBI_insert_REV"
     primer_bind     complement(59..81)
                     /created_by="primer3"
                     /annotation_group="3839095884236826495d2-31da-4321-8fab-45
                     c328d31fa4"
                     /%GC="52.2"
                     /Tm="58.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CTAGACCCTCACTTCCTACTCAG"
                     /Transferred_From="pIVTRup_BsmBI_insert_REV"
                     /Transferred_Similarity="100.00%"
                     /label="pIVTRup_BsmBI_insert_REV"
     3'UTR           82..136
                     /created_by="User"
                     /label="polyA(55)"
     misc_feature    complement(138..143)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     protein_bind    282..298
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     300..320
                     /%GC="57.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="62.7"
                     /annotation_group="14897190477113d16d283c-3d11-4023-bc08-f
                     f280cc6e51c"
                     /Sequence="TCCACACAACATACGAGCCGG"
                     /created_by="primer3"
                     /Transferred_From="PCR_0227"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0227"
     primer_bind     301..318
                     /created_by="primer3"
                     /annotation_group="36984589767678bff533c-a2d1-479e-9d44-51
                     3770d42716"
                     /%GC="55.6"
                     /Tm="55.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCACACAACATACGAGCC"
                     /Extension="ACTGGCGAACTACTTACTCTAGCTTCCCGG"
                     /Transferred_From="PCR_0096"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0096"
     primer_bind     301..318
                     /created_by="primer3"
                     /annotation_group="3866345085903c9e096e3-6705-4c76-90bf-d4
                     56713b7775"
                     /%GC="55.6"
                     /Tm="55.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCACACAACATACGAGCC"
                     /Extension="TGTGGTGATGTTGAAGAAAACCCTGGTCCA"
                     /Transferred_From="PCR_0099"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0099"
     promoter        complement(306..336)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     primer_bind     complement(323..343)
                     /created_by="primer3"
                     /annotation_group="29021356059983a94ec65-b0d0-4a19-aa0e-1a
                     b44de0a424"
                     /%GC="47.6"
                     /Tm="57.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCCAGGCTTTACACTTTATGC"
                     /Transferred_From="PCR_0122"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0122"
     protein_bind    351..372
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     primer_bind     complement(485..504)
                     /created_by="primer3"
                     /annotation_group="2184603152205ec342b70-a26d-4f1d-b24f-af
                     f7f915755a"
                     /%GC="60.0"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="9.1"
                     /Sequence="CGAGTCAGTGAGCGAGGAAG"
                     /Transferred_From="PCR_0083"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0083"
     primer_bind     complement(485..504)
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="9.1"
                     /Tm="60.2"
                     /annotation_group="18623645475372d235b3e4-a5f3-4c2e-bc07-f
                     e95be19c895"
                     /Sequence="CGAGTCAGTGAGCGAGGAAG"
                     /created_by="primer3"
                     /Transferred_From="PCR_0179"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0179"
     primer_bind     complement(485..504)
                     /created_by="primer3"
                     /annotation_group="199002396523304031c3fe-a772-4906-86ef-6
                     65c17b18f7c"
                     /%GC="60.0"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="9.1"
                     /Sequence="CGAGTCAGTGAGCGAGGAAG"
                     /Transferred_From="86708_p13"
                     /Transferred_Similarity="100.00%"
                     /label="86708_p13"
     primer_bind     complement(588..608)
                     /created_by="primer3"
                     /annotation_group="71392632065632ccf44bd-cf86-4690-9c98-9b
                     765f09bc54"
                     /%GC="52.4"
                     /Tm="60.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GCTCACATGTTCTTTCCTGCG"
                     /Transferred_From="PCR_0101"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0101"
     rep_origin      complement(660..1248)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     complement(700..719)
                     /%GC="60.0"
                     /Hairpin_Tm="36.7"
                     /Self_Dimer_Tm="None"
                     /Tm="60.7"
                     /annotation_group="186221644628029350a970-59f1-4746-850a-0
                     57bfb356574"
                     /Sequence="CACCTCTGACTTGAGCGTCG"
                     /created_by="primer3"
                     /Transferred_From="PCR_0143"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0143"
     primer_bind     1572..1592
                     /created_by="primer3"
                     /annotation_group="16144090014920b566d140-61cd-4c10-8331-2
                     eb8628cbb82"
                     /%GC="52.4"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACCGGCTCCAGATTTATCAGC"
                     /Transferred_From="qPCR_002"
                     /Transferred_Similarity="100.00%"
                     /label="qPCR_002"
     primer_bind     1645..1699
                     /Sequence="TCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAG
                     TAGTTCGC"
                     /Transferred_From="LCR_0007"
                     /Transferred_Similarity="100.00%"
                     /label="LCR_0007"
     primer_bind     complement(1646..1673)
                     /created_by="primer3"
                     /annotation_group="132575348129027e99fccb-eb78-4124-873b-f
                     83151cb9b41"
                     /%GC="46.4"
                     /Tm="63.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAACAATTAATAGACTGGATGGAGGCGG"
                     /Transferred_From="PCR_0068"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0068"
     primer_bind     complement(1646..1673)
                     /created_by="primer3"
                     /annotation_group="92486562166338cbeaea3-8930-449e-8aec-ab
                     eabd4a0646"
                     /%GC="46.4"
                     /Tm="63.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAACAATTAATAGACTGGATGGAGGCGG"
                     /Transferred_From="PCR_0020"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0020"
     primer_bind     complement(1647..1673)
                     /created_by="primer3"
                     /annotation_group="38333681811863c20a6ba-4476-4e2c-99ee-2a
                     74bd114882"
                     /%GC="44.4"
                     /Tm="62.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAACAATTAATAGACTGGATGGAGGCG"
                     /Extension="GGCGAAAGGGGGATGTGCTGCAAGGCGATT"
                     /Transferred_From="PCR_0097"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0097"
     primer_bind     complement(1652..1683)
                     /Sequence="AGCTTCCCGGCAACAATTAATAGACTGGATGG"
                     /Transferred_From="LU-p0025"
                     /Transferred_Similarity="100.00%"
                     /label="LU-p0025"
     primer_bind     complement(1652..1683)
                     /Sequence="AGCTTCCCGGCAACAATTAATAGACTGGATGG"
                     /Transferred_From="LU-p0049"
                     /Transferred_Similarity="100.00%"
                     /label="LU-p0049"
     primer_bind     complement(1653..1683)
                     /Sequence="AGCTTCCCGGCAACAATTAATAGACTGGATG"
                     /Transferred_From="LU-p0061"
                     /Transferred_Similarity="100.00%"
                     /label="LU-p0061"
     primer_bind     complement(1653..1683)
                     /Sequence="AGCTTCCCGGCAACAATTAATAGACTGGATG"
                     /Transferred_From="LU-p0072"
                     /Transferred_Similarity="100.00%"
                     /label="LU-p0072"
     primer_bind     complement(1653..1683)
                     /Sequence="AGCTTCCCGGCAACAATTAATAGACTGGATG"
                     /Transferred_From="LU-p0077"
                     /Transferred_Similarity="100.00%"
                     /label="LU-p0077"
     primer_bind     1664..1691
                     /Sequence="TTAATTGTTGCCGGGAAGCTAGAGTAAG"
                     /Transferred_From="LU-p0024"
                     /Transferred_Similarity="100.00%"
                     /label="LU-p0024"
     primer_bind     1664..1691
                     /Sequence="TTAATTGTTGCCGGGAAGCTAGAGTAAG"
                     /Transferred_From="LU-p0048"
                     /Transferred_Similarity="100.00%"
                     /label="LU-p0048"
     primer_bind     1664..1691
                     /Sequence="TTAATTGTTGCCGGGAAGCTAGAGTAAG"
                     /Transferred_From="LU-p0060"
                     /Transferred_Similarity="100.00%"
                     /label="LU-p0060"
     primer_bind     1664..1691
                     /Sequence="TTAATTGTTGCCGGGAAGCTAGAGTAAG"
                     /Transferred_From="LU-p0076"
                     /Transferred_Similarity="100.00%"
                     /label="LU-p0076"
     primer_bind     1664..1697
                     /Sequence="TTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTC"
                     /Transferred_From="LU-p0073"
                     /Transferred_Similarity="100.00%"
                     /label="LU-p0073"
     primer_bind     1674..1702
                     /created_by="primer3"
                     /annotation_group="13059146314619c94b04bd-bc1b-466c-b903-4
                     79c2114076e"
                     /%GC="55.2"
                     /Tm="67.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCGGGAAGCTAGAGTAAGTAGTTCGCCAG"
                     /Transferred_From="PCR_0067"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0067"
     primer_bind     1674..1702
                     /created_by="primer3"
                     /annotation_group="9248127131574fe5eaa79-ceeb-48ef-b91f-1c
                     88b24e4487"
                     /%GC="55.2"
                     /Tm="67.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCGGGAAGCTAGAGTAAGTAGTTCGCCAG"
                     /Transferred_From="PCR_0015"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0015"
     primer_bind     complement(1755..1775)
                     /created_by="primer3"
                     /annotation_group="16143989415134908d7236-102a-45d6-9310-d
                     68160b202f2"
                     /%GC="52.4"
                     /Tm="59.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAAGCCATACCAAACGACGAG"
                     /Transferred_From="qPCR_001"
                     /Transferred_Similarity="100.00%"
                     /label="qPCR_001"
     rep_origin      complement(3435..3863)
                     /direction="LEFT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     misc_feature    3792..3810
                     /Sequence="TGGCAAGTGTAGCGGTCAC"
                     /Hairpin_Tm="36.2"
                     /Tm="60.3"
                     /Self_Dimer_Tm="None"
                     /%GC="57.9"
                     /modified_by="User"
                     /Transferred_From="PCR_0114"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0114"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     complement(3945..3965)
                     /created_by="primer3"
                     /annotation_group="362309375178310e68f09-b3fe-4d30-910b-2a
                     12d5b2cc6a"
                     /%GC="57.1"
                     /Tm="63.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AATCGCCTTGCAGCACATCCC"
                     /Transferred_From="PCR_0093"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0093"
     primer_bind     3954..3973
                     /created_by="primer3"
                     /annotation_group="1767994431312166ffa148-186b-4b97-bb03-4
                     ccfe730a4bf"
                     /%GC="50.0"
                     /Tm="58.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGCAAGGCGATTAAGTTGGG"
                     /Transferred_From="PCR_0128"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0128"
     promoter        4027..4045
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     4038..4056
                     /created_by="primer3"
                     /annotation_group="469766135504151c2235e-aa40-411f-965a-d3
                     843ff7d59a"
                     /%GC="52.6"
                     /Tm="57.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="5.4"
                     /Sequence="ACTATAGGGCGAATTGGGC"
                     /Transferred_From="pIVTRup_BsmBI_insert_FWD"
                     /Transferred_Similarity="100.00%"
                     /label="pIVTRup_BsmBI_insert_FWD"
     primer_bind     4038..4056
                     /created_by="primer3"
                     /annotation_group="3838985565578ef9a4a42-9ab2-442e-a12c-b0
                     61c25d7729"
                     /%GC="52.6"
                     /Tm="57.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="5.4"
                     /Sequence="ACTATAGGGCGAATTGGGC"
                     /Transferred_From="pIVTRup_BsmBI_insert_FWD"
                     /Transferred_Similarity="100.00%"
                     /label="pIVTRup_BsmBI_insert_FWD"
     promoter        4117..4135
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     cds             4134..>4155
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="101362 - pIVTRup Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="101362 - pIVTRup Copy, 101362 - pIVTRup
                     PCR DraI, Digestion of 101362 - pIVTRup PCR DraI -
                     Fragment 3"
                     /Transferred_Similarity="100.00%"
                     /label="3'UTR"
     misc_feature    4156..9768
                     /Source="referenceToDocument: urn:local:.:sf-br7sn3s"
                     /label="PCR_06_AncBE4max (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    4155^4156
                     /Original_Bases="AGTAAGAAGAAATATAAGAGCCACC"
                     /label="AGTAAGAAGAAATATAAGAGCCACC"
                     /note="Geneious type: Editing History Deletion"
     primer_bind     4181..4210
                     /Mismatches=0
                     /Extension="AGTAAGAAGAAATATAAGAGCCACC"
                     /created_by="primer3"
                     /annotation_group="87977970804843913778f-831c-422f-97a8-a1
                     48d5ca30cb"
                     /%GC="56.7"
                     /Tm="71.9"
                     /Hairpin_Tm="61.5"
                     /Self_Dimer_Tm="20.5"
                     /Sequence="ATGAAACGGACAGCCGACGGAAGCGAGTTC"
                     /label="AncBE4max_FWD"
     primer_bind     4181..4198
                     /Mismatches=0
                     /Extension="AGTAAGAAGAAATATAAGAGCCACC"
                     /created_by="primer3"
                     /annotation_group="87978492331114d401c32-9549-471a-ab08-5e
                     740d36a87e"
                     /%GC="55.6"
                     /Tm="57.7"
                     /Hairpin_Tm="61.5"
                     /Self_Dimer_Tm="11.7"
                     /Sequence="ATGAAACGGACAGCCGAC"
                     /label="AncBE4max_FWD-2"
     CDS             4217..4237
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     primer_bind     4395..4415
                     /created_by="primer3"
                     /annotation_group="384402549410564285998-7986-4439-b4ef-1f
                     c8757b34c1"
                     /%GC="52.4"
                     /Tm="60.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GCTCCAAGAACACCACAAAGC"
                     /Transferred_From="base_editing_valid35"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid35"
     primer_bind     4395..4415
                     /created_by="primer3"
                     /annotation_group="47031192909401b82a7f9-f72b-478f-a7e4-7f
                     2468040cb7"
                     /%GC="52.4"
                     /Tm="60.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GCTCCAAGAACACCACAAAGC"
                     /Transferred_From="base_editing_valid35"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid35"
     primer_bind     4866..4886
                     /created_by="primer3"
                     /annotation_group="3844121142665a71ae449-8b4e-46cf-9d34-c2
                     7f26111268"
                     /%GC="52.4"
                     /Tm="60.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AGTCTTGTCACTATCAGCGGC"
                     /Transferred_From="base_editing_valid36"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid36"
     primer_bind     4866..4886
                     /created_by="primer3"
                     /annotation_group="470324213738831c430e2-58b5-473d-b6af-0a
                     27c5e95b5d"
                     /%GC="52.4"
                     /Tm="60.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AGTCTTGTCACTATCAGCGGC"
                     /Transferred_From="base_editing_valid36"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid36"
     CDS             5018..9118
                     /codon_start=1
                     /product="nickase mutant of the Cas9 endonuclease from the
                      Streptococcus pyogenes Type II CRISPR/Cas system"
                     /note="generates RNA-guided single strand nicks in DNA due
                      to the D10A mutation in the RuvC catalytic domain"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEE
                     SFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHM
                     IKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKS
                     RRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDN
                     LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLL
                     KALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKL
                     NREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY
                     VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP
                     KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKED
                     YFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTL
                     FEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFL
                     KSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTV
                     KVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEH
                     PVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVL
                     TRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAG
                     FIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFY
                     KVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGK
                     ATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSM
                     PQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVA
                     KVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFE
                     LENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHK
                     HYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAP
                     AAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGD"
                     /label="Cas9(D10A)"
     primer_bind     5102..5122
                     /created_by="primer3"
                     /annotation_group="3175367124987c111c02d-3cc6-43e8-8918-ec
                     7383254757"
                     /%GC="47.6"
                     /Tm="59.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AAGAAATTCAAGGTGCTGGGC"
                     /Transferred_From="PCR_0336"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0336"
     primer_bind     5133..5153
                     /created_by="primer3"
                     /annotation_group="384305219665389f023fb-7620-4932-ba94-c2
                     8d897f1fbb"
                     /%GC="52.4"
                     /Tm="59.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGCACAGCATCAAGAAGAACC"
                     /Transferred_From="base_editing_valid24"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid24"
     primer_bind     5133..5153
                     /created_by="primer3"
                     /annotation_group="4702062590669ad3d2778-be34-48d3-b734-24
                     24b8ae8a8b"
                     /%GC="52.4"
                     /Tm="59.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GGCACAGCATCAAGAAGAACC"
                     /Transferred_From="base_editing_valid24"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid24"
     primer_bind     5213..5233
                     /created_by="primer3"
                     /annotation_group="38442120060857cbf36b1-63a9-463c-b618-ff
                     49e344d9cd"
                     /%GC="52.4"
                     /Tm="59.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACCGCCAGAAGAAGATACACC"
                     /Transferred_From="base_editing_valid37"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid37"
     primer_bind     5213..5233
                     /created_by="primer3"
                     /annotation_group="4703352324181fd2658ab-260c-4f3a-bed4-7b
                     13aa909a0b"
                     /%GC="52.4"
                     /Tm="59.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACCGCCAGAAGAAGATACACC"
                     /Transferred_From="base_editing_valid37"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid37"
     primer_bind     5365..5384
                     /created_by="primer3"
                     /annotation_group="6077824560283e0507322-d717-409a-8ee1-5b
                     75d8e3adc0"
                     /%GC="55.0"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CATCTTCGGCAACATCGTGG"
                     /Transferred_From="PCR_0268"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0268"
     primer_bind     5464..5483
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="8.6"
                     /Tm="60.0"
                     /annotation_group="1862277800837038f692b8-eb04-4948-b64f-d
                     1a85c05aa80"
                     /Sequence="CCTGCGGCTGATCTATCTGG"
                     /created_by="primer3"
                     /Transferred_From="PCR_0158"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0158"
     primer_bind     5583..5603
                     /%GC="52.4"
                     /Hairpin_Tm="34.0"
                     /Self_Dimer_Tm="13.8"
                     /Tm="60.0"
                     /annotation_group="18622283094638a733a439-0e9a-449e-a111-2
                     da6430d4c2d"
                     /Sequence="AGACCTACAACCAGCTGTTCG"
                     /created_by="primer3"
                     /Transferred_From="PCR_0146"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0146"
     primer_bind     5708..5729
                     /created_by="primer3"
                     /annotation_group="38431469612265943b6d6-3bb1-4967-aede-0d
                     a3b859d18e"
                     /%GC="50.0"
                     /Tm="59.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.3"
                     /Sequence="GAGAAGAAGAATGGCCTGTTCG"
                     /Transferred_From="base_editing_valid25"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid25"
     primer_bind     5708..5729
                     /created_by="primer3"
                     /annotation_group="47021560431617963900c-247d-4bb7-8926-0e
                     4d1f956d2c"
                     /%GC="50.0"
                     /Tm="59.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.3"
                     /Sequence="GAGAAGAAGAATGGCCTGTTCG"
                     /Transferred_From="base_editing_valid25"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid25"
     primer_bind     5710..5730
                     /created_by="primer3"
                     /annotation_group="384430620863200fa00b0-1c28-4358-b2b2-52
                     8532f224e4"
                     /%GC="52.4"
                     /Tm="59.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.3"
                     /Sequence="GAAGAAGAATGGCCTGTTCGG"
                     /Transferred_From="base_editing_valid38"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid38"
     primer_bind     5710..5730
                     /created_by="primer3"
                     /annotation_group="470344436133321610810-10ca-4541-bdef-cc
                     e47ae5349d"
                     /%GC="52.4"
                     /Tm="59.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="3.3"
                     /Sequence="GAAGAAGAATGGCCTGTTCGG"
                     /Transferred_From="base_editing_valid38"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid38"
     primer_bind     5937..5957
                     /created_by="primer3"
                     /annotation_group="607790938333457d2f506-30bd-4399-aa04-76
                     7ba6d60f22"
                     /%GC="52.4"
                     /Tm="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGAACACCGAGATCACCAAGG"
                     /Transferred_From="PCR_0269"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0269"
     primer_bind     6111..6130
                     /%GC="60.0"
                     /Hairpin_Tm="41.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.4"
                     /annotation_group="18622814256743f18adde7-0d47-48d4-9a0b-5
                     7b01a52770c"
                     /Sequence="GAGCCAGCCAGGAAGAGTTC"
                     /created_by="primer3"
                     /Transferred_From="PCR_0159"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0159"
     primer_bind     6183..6203
                     /created_by="primer3"
                     /annotation_group="3843229429703de8ed542-e4e7-492c-a27a-9a
                     75355526b4"
                     /%GC="52.4"
                     /Tm="59.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="6.0"
                     /Sequence="TCGTGAAGCTGAACAGAGAGG"
                     /Transferred_From="base_editing_valid26"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid26"
     primer_bind     6183..6203
                     /created_by="primer3"
                     /annotation_group="4702250489059b4703d1d-5a4b-4625-aa51-a2
                     364463ae02"
                     /%GC="52.4"
                     /Tm="59.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="6.0"
                     /Sequence="TCGTGAAGCTGAACAGAGAGG"
                     /Transferred_From="base_editing_valid26"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid26"
     primer_bind     6186..6206
                     /created_by="primer3"
                     /annotation_group="3844389983381fba5b2c4-4460-48c5-968e-3a
                     a5fa89a351"
                     /%GC="52.4"
                     /Tm="59.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="6.0"
                     /Sequence="TGAAGCTGAACAGAGAGGACC"
                     /Transferred_From="base_editing_valid39"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid39"
     primer_bind     6186..6206
                     /created_by="primer3"
                     /annotation_group="470353276855490316b8f-1154-4b12-b878-06
                     0f9aafa416"
                     /%GC="52.4"
                     /Tm="59.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="6.0"
                     /Sequence="TGAAGCTGAACAGAGAGGACC"
                     /Transferred_From="base_editing_valid39"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid39"
     primer_bind     6336..6355
                     /%GC="55.0"
                     /Hairpin_Tm="39.5"
                     /Self_Dimer_Tm="None"
                     /Tm="59.2"
                     /annotation_group="18622323068757574207ee-23f0-4777-a9bd-2
                     c4a3b4bd603"
                     /Sequence="AGAAGATCCTGACCTTCCGC"
                     /created_by="primer3"
                     /Transferred_From="PCR_0147"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0147"
     primer_bind     6416..6436
                     /created_by="primer3"
                     /annotation_group="607799462014221e108a0-94c6-4c77-aca2-e9
                     bb7aa785dd"
                     /%GC="52.4"
                     /Tm="60.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AAGAGCGAGGAAACCATCACC"
                     /Transferred_From="PCR_0270"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0270"
     primer_bind     6507..6527
                     /created_by="primer3"
                     /annotation_group="384331834586729eb0046-0057-49a7-9874-72
                     5c3dc2f6f4"
                     /%GC="52.4"
                     /Tm="60.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TCGATAAGAACCTGCCCAACG"
                     /Transferred_From="base_editing_valid27"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid27"
     primer_bind     6507..6527
                     /created_by="primer3"
                     /annotation_group="4702334391124fb0b7403-df5c-423e-ada5-82
                     dae10641e3"
                     /%GC="52.4"
                     /Tm="60.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TCGATAAGAACCTGCCCAACG"
                     /Transferred_From="base_editing_valid27"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid27"
     primer_bind     6663..6683
                     /created_by="primer3"
                     /annotation_group="3844492745715558a0f9c-1d69-4dff-963e-80
                     5a669ace52"
                     /%GC="52.4"
                     /Tm="60.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACCTGCTGTTCAAGACCAACC"
                     /Transferred_From="base_editing_valid40"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid40"
     primer_bind     6663..6683
                     /created_by="primer3"
                     /annotation_group="4703625541371146a19ee-4302-4914-bc10-20
                     398c4255b3"
                     /%GC="52.4"
                     /Tm="60.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACCTGCTGTTCAAGACCAACC"
                     /Transferred_From="base_editing_valid40"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid40"
     primer_bind     6786..6805
                     /%GC="55.0"
                     /Hairpin_Tm="31.8"
                     /Self_Dimer_Tm="None"
                     /Tm="59.8"
                     /annotation_group="186228565386548a30baf8-dea5-4607-9b29-2
                     80ae84ae6de"
                     /Sequence="TGGGCACATACCACGATCTG"
                     /created_by="primer3"
                     /Transferred_From="PCR_0160"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0160"
     primer_bind     6876..6896
                     /created_by="primer3"
                     /annotation_group="6078101488936dc0e8bd2-461b-48a5-8652-c5
                     890b8a8d58"
                     /%GC="52.4"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGACCCTGACACTGTTTGAGG"
                     /Transferred_From="PCR_0271"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0271"
     primer_bind     7056..7075
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="9.7"
                     /Tm="60.4"
                     /annotation_group="18622364013746317317f2-9def-4e9a-a898-1
                     bfcb12f87c6"
                     /Sequence="ATTTCCTGAAGTCCGACGGC"
                     /created_by="primer3"
                     /Transferred_From="PCR_0148"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0148"
     primer_bind     7077..7097
                     /created_by="primer3"
                     /annotation_group="3843398978034a0c490d3-a94c-4a24-96cc-b2
                     c9b0a0b697"
                     /%GC="47.6"
                     /Tm="59.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TCGCCAACAGAAACTTCATGC"
                     /Transferred_From="base_editing_valid28"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid28"
     primer_bind     7077..7097
                     /created_by="primer3"
                     /annotation_group="470246605883386423438-f7e5-45b5-962f-2d
                     5a274567ab"
                     /%GC="47.6"
                     /Tm="59.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TCGCCAACAGAAACTTCATGC"
                     /Transferred_From="base_editing_valid28"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid28"
     primer_bind     7089..7109
                     /created_by="primer3"
                     /annotation_group="3844588738930f65f380a-3dd5-4386-b443-50
                     ffb6ae97bb"
                     /%GC="52.4"
                     /Tm="61.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="20.4"
                     /Sequence="ACTTCATGCAGCTGATCCACG"
                     /Transferred_From="base_editing_valid41"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid41"
     primer_bind     7089..7109
                     /created_by="primer3"
                     /annotation_group="470370673539913506908-72b4-474e-9495-76
                     86c043d4f8"
                     /%GC="52.4"
                     /Tm="61.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="20.4"
                     /Sequence="ACTTCATGCAGCTGATCCACG"
                     /Transferred_From="base_editing_valid41"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid41"
     primer_bind     7368..7388
                     /created_by="primer3"
                     /annotation_group="6078187157030d987ff47-4138-43ef-a1be-73
                     b2dcb8fc0e"
                     /%GC="52.4"
                     /Tm="60.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="0.6"
                     /Sequence="AAGAGGGCATCAAAGAGCTGG"
                     /Transferred_From="PCR_0272"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0272"
     primer_bind     7491..7510
                     /%GC="55.0"
                     /Hairpin_Tm="37.2"
                     /Self_Dimer_Tm="None"
                     /Tm="59.7"
                     /annotation_group="18622902295890a710ce6d-1e05-4c39-9dae-5
                     074be2841c8"
                     /Sequence="AGGAACTGGACATCAACCGG"
                     /created_by="primer3"
                     /Transferred_From="PCR_0161"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0161"
     primer_bind     7563..7583
                     /created_by="primer3"
                     /annotation_group="384467589020818363493-39dc-470f-973c-c3
                     ee87193c00"
                     /%GC="52.4"
                     /Tm="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACTCCATCGACAACAAGGTGC"
                     /Transferred_From="base_editing_valid42"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid42"
     primer_bind     7563..7583
                     /created_by="primer3"
                     /annotation_group="4703803626398baef5485-a21e-4304-8fc8-0e
                     6d011947a9"
                     /%GC="52.4"
                     /Tm="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ACTCCATCGACAACAAGGTGC"
                     /Transferred_From="base_editing_valid42"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid42"
     primer_bind     7647..7667
                     /created_by="primer3"
                     /annotation_group="3843483123613edc154f5-cb9f-430d-9a78-42
                     1a7e29c139"
                     /%GC="52.4"
                     /Tm="60.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AGATGAAGAACTACTGGCGGC"
                     /Transferred_From="base_editing_valid29"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid29"
     primer_bind     7647..7667
                     /created_by="primer3"
                     /annotation_group="47025580540328cb7e40d-0eba-4867-974d-7d
                     1621a5e5c4"
                     /%GC="52.4"
                     /Tm="60.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AGATGAAGAACTACTGGCGGC"
                     /Transferred_From="base_editing_valid29"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid29"
     primer_bind     7738..7757
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.9"
                     /annotation_group="18622406776419b629052e-7467-4fab-b234-3
                     08f01fd3e20"
                     /Sequence="GAGCGAACTGGATAAGGCCG"
                     /created_by="primer3"
                     /Transferred_From="PCR_0149"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0149"
     primer_bind     7878..7898
                     /created_by="primer3"
                     /annotation_group="6078274721821bc74825c-ee54-40c3-a69c-e4
                     49ca00488a"
                     /%GC="52.4"
                     /Tm="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGATCACCCTGAAGTCCAAGC"
                     /Transferred_From="PCR_0273"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0273"
     primer_bind     8040..8059
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="16.6"
                     /Tm="59.8"
                     /annotation_group="186229429112435b9a12a3-d1a6-4a2b-8f8e-d
                     edb63135ea8"
                     /Sequence="TGTACGGCGACTACAAGGTG"
                     /created_by="primer3"
                     /Transferred_From="PCR_0162"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0162"
     primer_bind     8049..8069
                     /created_by="primer3"
                     /annotation_group="3844774475633dc20b192-74a7-491d-ad5b-bf
                     17461be2c9"
                     /%GC="52.4"
                     /Tm="60.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="11.6"
                     /Sequence="ACTACAAGGTGTACGACGTGC"
                     /Transferred_From="base_editing_valid43"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid43"
     primer_bind     8049..8069
                     /created_by="primer3"
                     /annotation_group="470388611977702fb98ff-4b78-406a-9bf9-a7
                     d3afe700fe"
                     /%GC="52.4"
                     /Tm="60.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="11.6"
                     /Sequence="ACTACAAGGTGTACGACGTGC"
                     /Transferred_From="base_editing_valid43"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid43"
     primer_bind     8149..8171
                     /created_by="primer3"
                     /annotation_group="38435757016262e25796e-399f-4da8-8c58-5e
                     28ef8b34cc"
                     /%GC="47.8"
                     /Tm="60.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TTTCAAGACCGAGATTACCCTGG"
                     /Transferred_From="base_editing_valid30"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid30"
     primer_bind     8149..8171
                     /created_by="primer3"
                     /annotation_group="4702655836927d0d2bc3d-de34-41aa-9211-de
                     54f4cd9a42"
                     /%GC="47.8"
                     /Tm="60.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TTTCAAGACCGAGATTACCCTGG"
                     /Transferred_From="base_editing_valid30"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid30"
     primer_bind     8387..8408
                     /created_by="primer3"
                     /annotation_group="6079075914859545368f5-7a92-45c9-91b1-4f
                     91641946be"
                     /%GC="54.5"
                     /Tm="59.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="13.0"
                     /Sequence="GACTGGGACCCTAAGAAGTACG"
                     /Transferred_From="PCR_0281"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0281"
     primer_bind     8391..8410
                     /%GC="60.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="2.1"
                     /Tm="59.5"
                     /annotation_group="1862244397526302d58095-bf32-4d5a-ab8d-d
                     f4c9d7f7054"
                     /Sequence="GGGACCCTAAGAAGTACGGC"
                     /created_by="primer3"
                     /Transferred_From="PCR_0150"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0150"
     primer_bind     8487..8507
                     /created_by="primer3"
                     /annotation_group="607836247125603079bb4-422c-49de-9bdc-42
                     f1ae64722e"
                     /%GC="52.4"
                     /Tm="60.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="7.0"
                     /Sequence="AGAGTGTGAAAGAGCTGCTGG"
                     /Transferred_From="PCR_0274"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0274"
     primer_bind     8607..8627
                     /created_by="primer3"
                     /annotation_group="3844846721238447651ed-bead-4f53-b302-16
                     68c9adebba"
                     /%GC="52.4"
                     /Tm="59.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGCCTAAGTACTCCCTGTTCG"
                     /Transferred_From="base_editing_valid44"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid44"
     primer_bind     8607..8627
                     /created_by="primer3"
                     /annotation_group="4703983389071d78be32e-fd73-45d7-a8bc-d9
                     2524fc3610"
                     /%GC="52.4"
                     /Tm="59.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGCCTAAGTACTCCCTGTTCG"
                     /Transferred_From="base_editing_valid44"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid44"
     primer_bind     8710..8730
                     /created_by="primer3"
                     /annotation_group="3843668478361b262af5e-a53c-4b2c-aa7e-8c
                     d18b9ffd60"
                     /%GC="52.4"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGTGAACTTCCTGTACCTGGC"
                     /Transferred_From="base_editing_valid31"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid31"
     primer_bind     8710..8730
                     /created_by="primer3"
                     /annotation_group="47027507116719f477b29-9783-4d4d-a767-8d
                     e63f70f118"
                     /%GC="52.4"
                     /Tm="59.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGTGAACTTCCTGTACCTGGC"
                     /Transferred_From="base_editing_valid31"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid31"
     primer_bind     8722..8741
                     /%GC="60.0"
                     /Hairpin_Tm="41.9"
                     /Self_Dimer_Tm="28.4"
                     /Tm="60.2"
                     /annotation_group="18622981187591e83213f2-f9dd-40eb-898e-f
                     4e2782be3e2"
                     /Sequence="GTACCTGGCCAGCCACTATG"
                     /created_by="primer3"
                     /Transferred_From="PCR_0163"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0163"
     primer_bind     8925..8945
                     /created_by="primer3"
                     /annotation_group="607846050364116af3471-3103-49c2-9f0d-38
                     199f4c4dee"
                     /%GC="52.4"
                     /Tm="59.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AGCAGGCCGAGAATATCATCC"
                     /Transferred_From="PCR_0275"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0275"
     primer_bind     8928..8948
                     /created_by="primer3"
                     /annotation_group="384492673419379bd7d75-08ed-4eaa-8b57-cb
                     bc6ee1a6ad"
                     /%GC="52.4"
                     /Tm="59.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AGGCCGAGAATATCATCCACC"
                     /Transferred_From="base_editing_valid45"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid45"
     primer_bind     8928..8948
                     /created_by="primer3"
                     /annotation_group="4704072775595ed4008f6-4ecd-4c82-b5a9-2a
                     8135c036a6"
                     /%GC="52.4"
                     /Tm="59.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AGGCCGAGAATATCATCCACC"
                     /Transferred_From="base_editing_valid45"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid45"
     primer_bind     9012..9031
                     /%GC="60.0"
                     /Hairpin_Tm="38.9"
                     /Self_Dimer_Tm="0.4"
                     /Tm="60.0"
                     /annotation_group="1862248511743492206ef1-b659-45ab-9487-4
                     0a74e178a41"
                     /Sequence="GGAAGAGGTACACCAGCACC"
                     /created_by="primer3"
                     /Transferred_From="PCR_0151"
                     /Transferred_Similarity="100.00%"
                     /label="PCR_0151"
     CDS             9149..9397
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus
                      subtilis bacteriophage (Mol et al., 1995)"
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
                     /label="UGI"
     CDS             9428..9676
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus
                      subtilis bacteriophage (Mol et al., 1995)"
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
                     /label="UGI"
     primer_bind     9443..9463
                     /created_by="primer3"
                     /annotation_group="38449992751391870fe59-7922-4e6d-bdb2-b9
                     c0211ea7a9"
                     /%GC="52.4"
                     /Tm="59.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATCATCGAGAAGGAGACAGGC"
                     /Transferred_From="base_editing_valid46"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid46"
     primer_bind     9443..9463
                     /created_by="primer3"
                     /annotation_group="4704151795408b2b98d77-8b48-4ea6-bf27-9c
                     b8f4a3dca4"
                     /%GC="52.4"
                     /Tm="59.3"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATCATCGAGAAGGAGACAGGC"
                     /Transferred_From="base_editing_valid46"
                     /Transferred_Similarity="100.00%"
                     /label="base_editing_valid46"
     misc_feature    complement(9710..9739)
                     /Mismatches=0
                     /Extension="AGGCAAGCCCCGCAGAAGGCAGCGATTCA"
                     /created_by="primer3"
                     /annotation_group="8797919852522eebfca49-af47-4224-86f5-51
                     f541ad4056"
                     /%GC="46.7"
                     /Tm="65.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GACTTTCCTCTTCTTCTTGGGCTCGAATTC"
                     /label="AncBE4max_REV"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(9718..9739)
                     /Mismatches=0
                     /Extension="AGGCAAGCCCCGCAGAAGGCAGCGATTCA"
                     /created_by="primer3"
                     /annotation_group="8797966571901079680f6-2316-44c1-bcc3-c6
                     e62b0cd658"
                     /%GC="50.0"
                     /Tm="58.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GACTTTCCTCTTCTTCTTGGGC"
                     /label="AncBE4max_REV-2"
                     /note="Geneious type: primer_bind_reverse"
     CDS             9719..9739
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
ORIGIN      
        1 tctggccatg cccttcttct ctcccttgca cctgtacctc ttggtctttg aataaagcct
       61 gagtaggaag tgagggtcta gaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
      121 aaaaaaaaaa aaaaaatgag acgcgatcaa taaccgacac tcctgcgtcg cctacgcgct
      181 acgatctgca gaattccagc acactggcgg ccgttactag tggatccgag ctcggtacca
      241 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
      301 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
      361 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
      421 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
      481 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
      541 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
      601 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
      661 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
      721 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
      781 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
      841 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
      901 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
      961 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     1021 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     1081 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     1141 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     1201 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     1261 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     1321 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     1381 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     1441 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     1501 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     1561 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     1621 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     1681 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     1741 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     1801 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     1861 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     1921 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     1981 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     2041 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     2101 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     2161 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     2221 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     2281 ctcttccttt ttcaattcag aagaactcgt caagaaggcg atagaaggcg atgcgctgcg
     2341 aatcgggagc ggcgataccg taaagcacga ggaagcggtc agcccattcg ccgccaagct
     2401 cttcagcaat atcacgggta gccaacgcta tgtcctgata gcggtccgcc acacccagcc
     2461 ggccacagtc gatgaatcca gaaaagcggc cattttccac catgatattc ggcaagcagg
     2521 catcgccatg ggtcacgacg agatcctcgc cgtcgggcat gcgcgccttg agcctggcga
     2581 acagttcggc tggcgcgagc ccctgatgct cttcgtccag atcatcctga tcgacaagac
     2641 cggcttccat ccgagtacgt gctcgctcga tgcgatgttt cgcttggtgg tcgaatgggc
     2701 aggtagccgg atcaagcgta tgcagccgcc gcattgcatc agccatgatg gatactttct
     2761 cggcaggagc aaggtgggat gacaggagat cctgccccgg cacttcgccc aatagcagcc
     2821 agtcccttcc cgcttcagtg acaacgtcga gcacagctgc gcaaggaacg cccgtcgtgg
     2881 ccagccacga tagccgcgct gcctcgtcct gcagttcatt cagggcaccg gacaggtcgg
     2941 tcttgacaaa aagaaccggg cgcccctgcg ctgacagccg gaacacggcg gcatcagagc
     3001 agccgattgt ctgttgtgcc cagtcatagc cgaatagcct ctccacccaa gcggccggag
     3061 aacctgcgtg caatccatct tgttcaatca tgcgaaacga tcctcatcct gtctcttgat
     3121 cagatcttga tcccctgcgc catcagatcc ttggcggcaa gaaagccatc cagtttactt
     3181 tgcagggctt cccaacctta ccagagggcg ccccagctgg caattccggt tcgcttgctg
     3241 tccataaaac cgcccagtct agctatcgcc atgtaagccc actgcaagct acctgctttc
     3301 tctttgcgct tgcgttttcc cttgtccaga tagcccagta gctgacattc atccggggtc
     3361 agcaccgttt ctgcggactg gctttctacg tgttccgctt cctttagcag cccttgcgcc
     3421 ctgaattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt ttaaccaata
     3481 ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag ggttgagtgt
     3541 tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg tcaaagggcg
     3601 aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat caagtttttt
     3661 ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc gatttagagc
     3721 ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga aaggagcggg
     3781 cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct
     3841 taatgcgccg ctacagggcg cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg
     3901 cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     3961 cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     4021 gaattgtaat acgactcact atagggcgaa ttgggccctc tagatgcatg ctcgagcggc
     4081 cgccagtgtg atggatttgg accctcgtac agaagctaat acgactcact atagggaaat
     4141 aagagagaaa agaagagtaa gaagaaatat aagagccacc atgaaacgga cagccgacgg
     4201 aagcgagttc gagtcaccaa agaagaagcg gaaagtcagc agtgaaaccg gaccagtggc
     4261 agtggaccca accctgagga gacggattga gccccatgaa tttgaagtgt tctttgaccc
     4321 aagggagctg aggaaggaga catgcctgct gtacgagatc aagtggggca caagccacaa
     4381 gatctggcgc cacagctcca agaacaccac aaagcacgtg gaagtgaatt tcatcgagaa
     4441 gtttacctcc gagcggcact tctgcccctc taccagctgt tccatcacat ggtttctgtc
     4501 ttggagccct tgcggcgagt gttccaaggc catcaccgag ttcctgtctc agcaccctaa
     4561 cgtgaccctg gtcatctacg tggcccggct gtatcaccac atggaccagc agaacaggca
     4621 gggcctgcgc gatctggtga attctggcgt gaccatccag atcatgacag ccccagagta
     4681 cgactattgc tggcggaact tcgtgaatta tccacctggc aaggaggcac actggccaag
     4741 atacccaccc ctgtggatga agctgtatgc actggagctg cacgcaggaa tcctgggcct
     4801 gcctccatgt ctgaatatcc tgcggagaaa gcagccccag ctgacatttt tcaccattgc
     4861 tctgcagtct tgtcactatc agcggctgcc tcctcatatt ctgtgggcta caggcctgaa
     4921 gtctggagga tctagcggag gatcctctgg cagcgagaca ccaggaacaa gcgagtcagc
     4981 aacaccagag agcagtggcg gcagcagcgg cggcagcgac aagaagtaca gcatcggcct
     5041 ggccatcggc accaactctg tgggctgggc cgtgatcacc gacgagtaca aggtgcccag
     5101 caagaaattc aaggtgctgg gcaacaccga ccggcacagc atcaagaaga acctgatcgg
     5161 agccctgctg ttcgacagcg gcgaaacagc cgaggccacc cggctgaaga gaaccgccag
     5221 aagaagatac accagacgga agaaccggat ctgctatctg caagagatct tcagcaacga
     5281 gatggccaag gtggacgaca gcttcttcca cagactggaa gagtccttcc tggtggaaga
     5341 ggataagaag cacgagcggc accccatctt cggcaacatc gtggacgagg tggcctacca
     5401 cgagaagtac cccaccatct accacctgag aaagaaactg gtggacagca ccgacaaggc
     5461 cgacctgcgg ctgatctatc tggccctggc ccacatgatc aagttccggg gccacttcct
     5521 gatcgagggc gacctgaacc ccgacaacag cgacgtggac aagctgttca tccagctggt
     5581 gcagacctac aaccagctgt tcgaggaaaa ccccatcaac gccagcggcg tggacgccaa
     5641 ggccatcctg tctgccagac tgagcaagag cagacggctg gaaaatctga tcgcccagct
     5701 gcccggcgag aagaagaatg gcctgttcgg aaacctgatt gccctgagcc tgggcctgac
     5761 ccccaacttc aagagcaact tcgacctggc cgaggatgcc aaactgcagc tgagcaagga
     5821 cacctacgac gacgacctgg acaacctgct ggcccagatc ggcgaccagt acgccgacct
     5881 gtttctggcc gccaagaacc tgtccgacgc catcctgctg agcgacatcc tgagagtgaa
     5941 caccgagatc accaaggccc ccctgagcgc ctctatgatc aagagatacg acgagcacca
     6001 ccaggacctg accctgctga aagctctcgt gcggcagcag ctgcctgaga agtacaaaga
     6061 gattttcttc gaccagagca agaacggcta cgccggctac attgacggcg gagccagcca
     6121 ggaagagttc tacaagttca tcaagcccat cctggaaaag atggacggca ccgaggaact
     6181 gctcgtgaag ctgaacagag aggacctgct gcggaagcag cggaccttcg acaacggcag
     6241 catcccccac cagatccacc tgggagagct gcacgccatt ctgcggcggc aggaagattt
     6301 ttacccattc ctgaaggaca accgggaaaa gatcgagaag atcctgacct tccgcatccc
     6361 ctactacgtg ggccctctgg ccaggggaaa cagcagattc gcctggatga ccagaaagag
     6421 cgaggaaacc atcaccccct ggaacttcga ggaagtggtg gacaagggcg cttccgccca
     6481 gagcttcatc gagcggatga ccaacttcga taagaacctg cccaacgaga aggtgctgcc
     6541 caagcacagc ctgctgtacg agtacttcac cgtgtataac gagctgacca aagtgaaata
     6601 cgtgaccgag ggaatgagaa agcccgcctt cctgagcggc gagcagaaaa aggccatcgt
     6661 ggacctgctg ttcaagacca accggaaagt gaccgtgaag cagctgaaag aggactactt
     6721 caagaaaatc gagtgcttcg actccgtgga aatctccggc gtggaagatc ggttcaacgc
     6781 ctccctgggc acataccacg atctgctgaa aattatcaag gacaaggact tcctggacaa
     6841 tgaggaaaac gaggacattc tggaagatat cgtgctgacc ctgacactgt ttgaggacag
     6901 agagatgatc gaggaacggc tgaaaaccta tgcccacctg ttcgacgaca aagtgatgaa
     6961 gcagctgaag cggcggagat acaccggctg gggcaggctg agccggaagc tgatcaacgg
     7021 catccgggac aagcagtccg gcaagacaat cctggatttc ctgaagtccg acggcttcgc
     7081 caacagaaac ttcatgcagc tgatccacga cgacagcctg acctttaaag aggacatcca
     7141 gaaagcccag gtgtccggcc agggcgatag cctgcacgag cacattgcca atctggccgg
     7201 cagccccgcc attaagaagg gcatcctgca gacagtgaag gtggtggacg agctcgtgaa
     7261 agtgatgggc cggcacaagc ccgagaacat cgtgatcgaa atggccagag agaaccagac
     7321 cacccagaag ggacagaaga acagccgcga gagaatgaag cggatcgaag agggcatcaa
     7381 agagctgggc agccagatcc tgaaagaaca ccccgtggaa aacacccagc tgcagaacga
     7441 gaagctgtac ctgtactacc tgcagaatgg gcgggatatg tacgtggacc aggaactgga
     7501 catcaaccgg ctgtccgact acgatgtgga ccatatcgtg cctcagagct ttctgaagga
     7561 cgactccatc gacaacaagg tgctgaccag aagcgacaag aaccggggca agagcgacaa
     7621 cgtgccctcc gaagaggtcg tgaagaagat gaagaactac tggcggcagc tgctgaacgc
     7681 caagctgatt acccagagaa agttcgacaa tctgaccaag gccgagagag gcggcctgag
     7741 cgaactggat aaggccggct tcatcaagag acagctggtg gaaacccggc agatcacaaa
     7801 gcacgtggca cagatcctgg actcccggat gaacactaag tacgacgaga atgacaagct
     7861 gatccgggaa gtgaaagtga tcaccctgaa gtccaagctg gtgtccgatt tccggaagga
     7921 tttccagttt tacaaagtgc gcgagatcaa caactaccac cacgcccacg acgcctacct
     7981 aaacgccgtc gtgggaaccg ccctgatcaa aaagtaccct aagctggaaa gcgagttcgt
     8041 gtacggcgac tacaaggtgt acgacgtgcg gaagatgatc gccaagagcg agcaggaaat
     8101 cggcaaggct accgccaagt acttcttcta cagcaacatc atgaactttt tcaagaccga
     8161 gattaccctg gccaacggcg agatccggaa gcggcctctg atcgagacaa acggcgaaac
     8221 cggggagatc gtgtgggata agggccggga ttttgccacc gtgcggaaag tgctgagcat
     8281 gccccaagtg aatatcgtga aaaagaccga ggtgcagaca ggcggcttca gcaaagagtc
     8341 tatcctgccc aagaggaaca gcgataagct gatcgccaga aagaaggact gggaccctaa
     8401 gaagtacggc ggcttcgaca gccccaccgt ggcctattct gtgctggtgg tggccaaagt
     8461 ggaaaagggc aagtccaaga aactgaagag tgtgaaagag ctgctgggga tcaccatcat
     8521 ggaaagaagc agcttcgaga agaatcccat cgactttctg gaagccaagg gctacaaaga
     8581 agtgaaaaag gacctgatca tcaagctgcc taagtactcc ctgttcgagc tggaaaacgg
     8641 ccggaagaga atgctggcct ctgccggcga actgcagaag ggaaacgaac tggccctgcc
     8701 ctccaaatat gtgaacttcc tgtacctggc cagccactat gagaagctga agggctcccc
     8761 cgaggataat gagcagaaac agctgtttgt ggaacagcac aagcactacc tggacgagat
     8821 catcgagcag atcagcgagt tctccaagag agtgatcctg gccgacgcta atctggacaa
     8881 agtgctgtcc gcctacaaca agcaccggga taagcccatc agagagcagg ccgagaatat
     8941 catccacctg tttaccctga ccaatctggg agcccctgcc gccttcaagt actttgacac
     9001 caccatcgac cggaagaggt acaccagcac caaagaggtg ctggacgcca ccctgatcca
     9061 ccagagcatc accggcctgt acgagacacg gatcgacctg tctcagctgg gaggtgacag
     9121 cggcgggagc ggcgggagcg gggggagcac taatctgagc gacatcattg agaaggagac
     9181 tgggaaacag ctggtcattc aggagtccat cctgatgctg cctgaggagg tggaggaagt
     9241 gatcggcaac aagccagagt ctgacatcct ggtgcacacc gcctacgacg agtccacaga
     9301 tgagaatgtg atgctgctga cctctgacgc ccccgagtat aagccttggg ccctggtcat
     9361 ccaggattct aacggcgaga ataagatcaa gatgctgagc ggaggatccg gaggatctgg
     9421 aggcagcacc aacctgtctg acatcatcga gaaggagaca ggcaagcagc tggtcatcca
     9481 ggagagcatc ctgatgctgc ccgaagaagt cgaagaagtg atcggaaaca agcctgagag
     9541 cgatatcctg gtccataccg cctacgacga gagtaccgac gaaaatgtga tgctgctgac
     9601 atccgacgcc ccagagtata agccctgggc tctggtcatc caggattcca acggagagaa
     9661 caaaatcaaa atgctgtctg gcggctcaaa aagaaccgcc gacggcagcg aattcgagcc
     9721 caagaagaag aggaaagtct gaatcgctgc cttctgcggg gcttgcct
//
