LOCUS       pCART0003_pIVTRup(BsmBI)_ABEmax7.10-SpRY_        9627 bp    DNA     circular UNA 08-JUL-2021
DEFINITION  Gibson Assembly ligation product.
ACCESSION   urn.local...11-f8h106j
VERSION     urn.local...11-f8h106j
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        75..93
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     promoter        165..183
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     cds             182..>228
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="101362 - pIVTRup Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="101362 - pIVTRup Copy, 101362 - pIVTRup
                     PCR DraI, Digestion of 101362 - pIVTRup PCR DraI -
                     Fragment 3"
                     /Transferred_Similarity="100.00%"
                     /label="3'UTR"
     misc_feature    complement(203..228)
                     /Sequence="GGTGGCTCTTATATTTCTTCTTACTC"
                     /Hairpin_Tm="None"
                     /Tm="57.4"
                     /Self_Dimer_Tm="None"
                     /%GC="38.5"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="Linearize_REV"
                     /note="Geneious type: primer_bind_reverse"
     ligation        204..228
                     /label="Ligation"
     misc_feature    204..5675
                     /Source="referenceToDocument: urn:local:.:1w-f8h106t"
                     /label="140003-addgene_pCMV-T7-ABEmax(7.10)-SpRY-P2A-EGFP
                     (RTW5025) PCR Product (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     primer_bind     204..228
                     /Sequence="AGTAAGAAGAAATATAAGAGCCACC"
                     /Hairpin_Tm="None"
                     /Tm="56.4"
                     /Self_Dimer_Tm="None"
                     /%GC="36.0"
                     /created_by="primer3"
                     /label="GA_overhang"
     CDS             223..228
                     /Color="#c3d9ff"
                     /modified_by="User"
                     /label="Kozak sequence"
     source          <229..>5646
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     229..251
                     /Sequence="ATGAAACGGACAGCCGACGGAAG"
                     /Hairpin_Tm="61.5"
                     /Tm="65.4"
                     /Self_Dimer_Tm="20.5"
                     /%GC="56.5"
                     /created_by="primer3"
                     /Extension="AGTAAGAAGAAATATAAGAGCCACC"
                     /label="prEP0141_SPRY-ABE-CBE-FWD "
     CDS             265..285
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             1474..3189
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes
                     Cas9 (Zetsche et al., 2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEE
                     SFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHM
                     IKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKS
                     RRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDN
                     LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLL
                     KALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKL
                     NREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY
                     VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP
                     KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKED
                     YFKKIE"
                     /label="Cas9(N)"
     CDS             5617..5637
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     primer_bind     complement(5619..5646)
                     /Sequence="TCCGCTTCCGACTTTCCTCTTCTTCTTG"
                     /Hairpin_Tm="36.3"
                     /Tm="66.5"
                     /Self_Dimer_Tm="None"
                     /%GC="50.0"
                     /created_by="primer3"
                     /Extension="AGGCAAGCCCCGCAGAAGGCAGCGATTCA"
                     /label="prEP0143_SPRY-ABE-REV "
     ligation        5650..5675
                     /label="Ligation"
     misc_feature    5650..228
                     /Source="referenceToDocument: urn:local:.:1x-f8h106t"
                     /label="PD0047_pIVTRup(BsmBI)_modified-from-Addgene-101362
                     PCR Product (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     cds             5650..5756
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="101362 - pIVTRup Copy; dCas9-VP64"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="101362 - pIVTRup Copy, 101362 - pIVTRup
                     PCR DraI, Digestion of 101362 - pIVTRup PCR DraI -
                     Fragment 3, dCas9-VP64"
                     /Transferred_Similarity="100.00%"
                     /label="5'UTR"
     primer_bind     complement(5650..5675)
                     /Sequence="AGGCAAGCCCCGCAGAAGGCAGCGAT"
                     /Hairpin_Tm="52.2"
                     /Tm="74.4"
                     /Self_Dimer_Tm="7.6"
                     /%GC="65.4"
                     /created_by="primer3"
                     /label="GA_overhang"
     primer_bind     5650..5665
                     /Sequence="ATCGCTGCCTTCTGCG"
                     /Hairpin_Tm="53.8"
                     /Tm="57.2"
                     /Self_Dimer_Tm="9.7"
                     /%GC="62.5"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="Linearize_FWD"
     3'UTR           5757..5811
                     /created_by="User"
                     /label="polyA(55)"
     misc_feature    complement(5813..5818)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     protein_bind    5957..5973
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(5981..6011)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6026..6047
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6335..6923)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     rep_origin      complement(9110..9538)
                     /direction="LEFT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN      
        1 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
       61 cggccagtga attgtaatac gactcactat agggcgaatt gggccctcta gatgcatgct
      121 cgagcggccg ccagtgtgat ggatttggac cctcgtacag aagctaatac gactcactat
      181 agggaaataa gagagaaaag aagagtaaga agaaatataa gagccaccat gaaacggaca
      241 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctctga agtcgagttt
      301 agccacgagt attggatgag gcacgcactg accctggcaa agcgagcatg ggatgaaaga
      361 gaagtccccg tgggcgccgt gctggtgcac aacaatagag tgatcggaga gggatggaac
      421 aggccaatcg gccgccacga ccctaccgca cacgcagaga tcatggcact gaggcaggga
      481 ggcctggtca tgcagaatta ccgcctgatc gatgccaccc tgtatgtgac actggagcca
      541 tgcgtgatgt gcgcaggagc aatgatccac agcaggatcg gaagagtggt gttcggagca
      601 cgggacgcca agaccggcgc agcaggctcc ctgatggatg tgctgcacca ccccggcatg
      661 aaccaccggg tggagatcac agagggaatc ctggcagacg agtgcgccgc cctgctgagc
      721 gatttcttta gaatgcggag acaggagatc aaggcccaga agaaggcaca gagctccacc
      781 gactctggag gatctagcgg aggatcctct ggaagcgaga caccaggcac aagcgagtcc
      841 gccacaccag agagctccgg cggctcctcc ggaggatcct ctgaggtgga gttttcccac
      901 gagtactgga tgagacatgc cctgaccctg gccaagaggg cacgcgatga gagggaggtg
      961 cctgtgggag ccgtgctggt gctgaacaat agagtgatcg gcgagggctg gaacagagcc
     1021 atcggcctgc acgacccaac agcccatgcc gaaattatgg ccctgagaca gggcggcctg
     1081 gtcatgcaga actacagact gattgacgcc accctgtacg tgacattcga gccttgcgtg
     1141 atgtgcgccg gcgccatgat ccactctagg atcggccgcg tggtgtttgg cgtgaggaac
     1201 gcaaaaaccg gcgccgcagg ctccctgatg gacgtgctgc actaccccgg catgaatcac
     1261 cgcgtcgaaa ttaccgaggg aatcctggca gatgaatgtg ccgccctgct gtgctatttc
     1321 tttcggatgc ctagacaggt gttcaatgct cagaagaagg cccagagctc caccgactcc
     1381 ggaggatcta gcggaggctc ctctggctct gagacacctg gcacaagcga gagcgcaaca
     1441 cctgaaagca gcgggggcag cagcgggggg tcagacaaga agtacagcat cggcctggcc
     1501 atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag
     1561 aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc
     1621 ctgctgttcg acagcggcga aacagccgag agaacccggc tgaagagaac cgccagaaga
     1681 agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg
     1741 gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat
     1801 aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag
     1861 aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac
     1921 ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc
     1981 gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag
     2041 acctacaacc agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc
     2101 atcctgtctg ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc
     2161 ggcgagaaga agaatggcct gttcggaaac ctgattgccc tgagcctggg cctgaccccc
     2221 aacttcaaga gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc
     2281 tacgacgacg acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt
     2341 ctggccgcca agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc
     2401 gagatcacca aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag
     2461 gacctgaccc tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt
     2521 ttcttcgacc agagcaagaa cggctacgcc ggctacattg acggcggagc cagccaggaa
     2581 gagttctaca agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc
     2641 gtgaagctga acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc
     2701 ccccaccaga tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac
     2761 ccattcctga aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac
     2821 tacgtgggcc ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag
     2881 gaaaccatca ccccctggaa cttcgaggaa gtggtggaca agggcgcttc cgcccagagc
     2941 ttcatcgagc ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag
     3001 cacagcctgc tgtacgagta cttcaccgtg tataacgagc tgaccaaagt gaaatacgtg
     3061 accgagggaa tgagaaagcc cgccttcctg agcggcgagc agaaaaaggc catcgtggac
     3121 ctgctgttca agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag
     3181 aaaatcgagt gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc
     3241 ctgggcacat accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag
     3301 gaaaacgagg acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag
     3361 atgatcgagg aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag
     3421 ctgaagcggc ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc
     3481 cgggacaagc agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac
     3541 agaaacttca tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa
     3601 gcccaggtgt ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc
     3661 cccgccatta agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg
     3721 atgggccggc acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc
     3781 cagaagggac agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag
     3841 ctgggcagcc agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag
     3901 ctgtacctgt actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc
     3961 aaccggctgt ccgactacga tgtggaccat atcgtgcctc agagctttct gaaggacgac
     4021 tccatcgaca acaaggtgct gaccagaagc gacaagaacc ggggcaagag cgacaacgtg
     4081 ccctccgaag aggtcgtgaa gaagatgaag aactactggc ggcagctgct gaacgccaag
     4141 ctgattaccc agagaaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa
     4201 ctggataagg ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac
     4261 gtggcacaga tcctggactc ccggatgaac actaagtacg acgagaatga caagctgatc
     4321 cgggaagtga aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc
     4381 cagttttaca aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac
     4441 gccgtcgtgg gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac
     4501 ggcgactaca aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc
     4561 aaggctaccg ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt
     4621 accctggcca acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaaccggg
     4681 gagatcgtgt gggataaggg ccgggatttt gccaccgtgc ggaaagtgct gagcatgccc
     4741 caagtgaata tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc
     4801 agacccaaga ggaacagcga taagctgatc gccagaaaga aggactggga ccctaagaag
     4861 tacggcggct tcctgtggcc caccgtggcc tattctgtgc tggtggtggc caaagtggaa
     4921 aagggcaagt ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa
     4981 agaagcagct tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg
     5041 aaaaaggacc tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg
     5101 aagagaatgc tggcctctgc caagcagctg cagaagggaa acgaactggc cctgccctcc
     5161 aaatatgtga acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag
     5221 gataatgagc agaaacagct gtttgtggaa cagcacaagc actacctgga cgagatcatc
     5281 gagcagatca gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaagtg
     5341 ctgtccgcct acaacaagca ccgggataag cccatcagag agcaggccga gaatatcatc
     5401 cacctgttta ccctgaccag actgggagcc cctagagcct tcaagtactt tgacaccacc
     5461 atcgacccca agcagtacag aagcaccaaa gaggtgctgg acgccaccct gatccaccag
     5521 agcatcaccg gcctgtacga gacacggatc gacctgtctc agctgggagg tgactctggc
     5581 ggctcaaaaa gaaccgccga cggcagcgaa ttcgagccca agaagaagag gaaagtcgga
     5641 agcggatgaa tcgctgcctt ctgcggggct tgccttctgg ccatgccctt cttctctccc
     5701 ttgcacctgt acctcttggt ctttgaataa agcctgagta ggaagtgagg gtctagaaaa
     5761 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa atgagacgcg
     5821 atcaataacc gacactcctg cgtcgcctac gcgctacgat ctgcagaatt ccagcacact
     5881 ggcggccgtt actagtggat ccgagctcgg taccaagctt ggcgtaatca tggtcatagc
     5941 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
     6001 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
     6061 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
     6121 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     6181 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     6241 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     6301 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     6361 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     6421 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     6481 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     6541 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     6601 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     6661 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     6721 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     6781 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     6841 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     6901 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     6961 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7021 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7081 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7141 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7201 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7261 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7321 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7381 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7441 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7501 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7561 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7621 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7681 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     7741 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     7801 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     7861 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     7921 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa ttcagaagaa
     7981 ctcgtcaaga aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag
     8041 cacgaggaag cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa
     8101 cgctatgtcc tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa
     8161 gcggccattt tccaccatga tattcggcaa gcaggcatcg ccatgggtca cgacgagatc
     8221 ctcgccgtcg ggcatgcgcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg
     8281 atgctcttcg tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg
     8341 ctcgatgcga tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag
     8401 ccgccgcatt gcatcagcca tgatggatac tttctcggca ggagcaaggt gggatgacag
     8461 gagatcctgc cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac
     8521 gtcgagcaca gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc
     8581 gtcctgcagt tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc
     8641 ctgcgctgac agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc
     8701 atagccgaat agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc
     8761 aatcatgcga aacgatcctc atcctgtctc ttgatcagat cttgatcccc tgcgccatca
     8821 gatccttggc ggcaagaaag ccatccagtt tactttgcag ggcttcccaa ccttaccaga
     8881 gggcgcccca gctggcaatt ccggttcgct tgctgtccat aaaaccgccc agtctagcta
     8941 tcgccatgta agcccactgc aagctacctg ctttctcttt gcgcttgcgt tttcccttgt
     9001 ccagatagcc cagtagctga cattcatccg gggtcagcac cgtttctgcg gactggcttt
     9061 ctacgtgttc cgcttccttt agcagccctt gcgccctgaa ttttgttaaa attcgcgtta
     9121 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
     9181 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
     9241 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
     9301 ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg taaagcacta
     9361 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc ggcgaacgtg
     9421 gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg
     9481 gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc
     9541 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
     9601 tacgccagct ggcgaaaggg ggatgtg
//
