LOCUS       pCART0002_pIVTRup(BsmBI)_CBE4max-SpRY_        9777 bp    DNA     circular UNA 08-JUL-2021
DEFINITION  Gibson Assembly ligation product.
ACCESSION   urn.local...10-f8h106j
VERSION     urn.local...10-f8h106j
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     promoter        75..93
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     promoter        165..183
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     cds             182..>228
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="101362 - pIVTRup Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="101362 - pIVTRup Copy, 101362 - pIVTRup
                     PCR DraI, Digestion of 101362 - pIVTRup PCR DraI -
                     Fragment 3"
                     /Transferred_Similarity="100.00%"
                     /label="3'UTR"
     misc_feature    complement(203..228)
                     /Sequence="GGTGGCTCTTATATTTCTTCTTACTC"
                     /Hairpin_Tm="None"
                     /Tm="57.4"
                     /Self_Dimer_Tm="None"
                     /%GC="38.5"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="Linearize_REV"
                     /note="Geneious type: primer_bind_reverse"
     ligation        204..228
                     /label="Ligation"
     misc_feature    204..5825
                     /Source="referenceToDocument: urn:local:.:1s-f8h106q"
                     /label="139999-addgene_pCAG-CBE4max-SpRY-P2A-EGFP
                     (RTW5133)  PCR Product (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     primer_bind     204..228
                     /Sequence="AGTAAGAAGAAATATAAGAGCCACC"
                     /Hairpin_Tm="None"
                     /Tm="56.4"
                     /Self_Dimer_Tm="None"
                     /%GC="36.0"
                     /created_by="primer3"
                     /label="GA_overhang"
     CDS             223..228
                     /Color="#c3d9ff"
                     /modified_by="User"
                     /label="Kozak sequence"
     source          <229..>5796
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label="source synthetic DNA construct"
     primer_bind     229..251
                     /Sequence="ATGAAACGGACAGCCGACGGAAG"
                     /Hairpin_Tm="61.5"
                     /Tm="65.4"
                     /Self_Dimer_Tm="20.5"
                     /%GC="56.5"
                     /created_by="primer3"
                     /Extension="AGTAAGAAGAAATATAAGAGCCACC"
                     /label="prEP0141_SPRY-ABE-CBE-FWD "
     primer_bind     229
                     /Sequence="A"
                     /created_by="epankevich"
                     /label="beginning of ORF"
     CDS             265..285
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     CDS             286..969
                     /codon_start=1
                     /product="cytidine deaminase (C to U editing enzyme) from
                     rat"
                     /note="can use ssDNA as a substrate (Komor et al., 2016)"
                     /translation="SSETGPVAVDPTLRRRIEPHEFEVFFDPRELRKETCLLYEINWG
                     GRHSIWRHTSQNTNKHVEVNFIEKFTTERYFCPNTRCSITWFLSWSPCGECSRAITEF
                     LSRYPHVTLFIYIARLYHHADPRNRQGLRDLISSGVTIQIMTEQESGYCWRNFVNYSP
                     SNEAHWPRYPHLWVRLYVLELYCIILGLPPCLNILRRKQPQLTFFTIALQSCHYQRLP
                     PHILWATGLK"
                     /label="APOBEC-1"
     CDS             1066..2781
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes
                     Cas9 (Zetsche et al., 2015)"
                     /translation="DKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAERTRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEE
                     SFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHM
                     IKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKS
                     RRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDN
                     LLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLL
                     KALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKL
                     NREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYY
                     VGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLP
                     KHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKED
                     YFKKIE"
                     /label="Cas9(N)"
     primer_bind     5196
                     /Sequence="C"
                     /created_by="epankevich"
                     /label="end of ORF"
     CDS             5197..5445
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus
                      subtilis bacteriophage (Mol et al., 1995)"
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
                     /label="UGI"
     CDS             5476..5724
                     /codon_start=1
                     /product="uracil-DNA glycosylase inhibitor from a Bacillus
                      subtilis bacteriophage (Mol et al., 1995)"
                     /translation="TNLSDIIEKETGKQLVIQESILMLPEEVEEVIGNKPESDILVHT
                     AYDESTDENVMLLTSDAPEYKPWALVIQDSNGENKIKML"
                     /label="UGI"
     CDS             5767..5787
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian
                     virus 40) large T antigen"
                     /translation="PKKKRKV"
                     /label="SV40 NLS"
     primer_bind     complement(5775..5796)
                     /Sequence="ACCGCCTCCGACTTTCCTCTTC"
                     /Hairpin_Tm="36.3"
                     /Tm="64.2"
                     /Self_Dimer_Tm="None"
                     /%GC="59.1"
                     /created_by="primer3"
                     /Extension="AGGCAAGCCCCGCAGAAGGCAGCGATTCA"
                     /label="prEP0144_SPRY-CBE-REV "
     ligation        5800..5825
                     /label="Ligation"
     misc_feature    5800..228
                     /Source="referenceToDocument: urn:local:.:1t-f8h106q"
                     /label="PD0047_pIVTRup(BsmBI)_modified-from-Addgene-101362
                     PCR Product (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     cds             5800..5906
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="101362 - pIVTRup Copy; dCas9-VP64"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="101362 - pIVTRup Copy, 101362 - pIVTRup
                     PCR DraI, Digestion of 101362 - pIVTRup PCR DraI -
                     Fragment 3, dCas9-VP64"
                     /Transferred_Similarity="100.00%"
                     /label="5'UTR"
     primer_bind     complement(5800..5825)
                     /Sequence="AGGCAAGCCCCGCAGAAGGCAGCGAT"
                     /Hairpin_Tm="52.2"
                     /Tm="74.4"
                     /Self_Dimer_Tm="7.6"
                     /%GC="65.4"
                     /created_by="primer3"
                     /label="GA_overhang"
     primer_bind     5800..5815
                     /Sequence="ATCGCTGCCTTCTGCG"
                     /Hairpin_Tm="53.8"
                     /Tm="57.2"
                     /Self_Dimer_Tm="9.7"
                     /%GC="62.5"
                     /created_by="primer3"
                     /modified_by="User"
                     /label="Linearize_FWD"
     3'UTR           5907..5961
                     /created_by="User"
                     /label="polyA(55)"
     misc_feature    complement(5963..5968)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     protein_bind    6107..6123
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     promoter        complement(6131..6161)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    6176..6197
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(6485..7073)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     rep_origin      complement(9260..9688)
                     /direction="LEFT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
ORIGIN      
        1 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
       61 cggccagtga attgtaatac gactcactat agggcgaatt gggccctcta gatgcatgct
      121 cgagcggccg ccagtgtgat ggatttggac cctcgtacag aagctaatac gactcactat
      181 agggaaataa gagagaaaag aagagtaaga agaaatataa gagccaccat gaaacggaca
      241 gccgacggaa gcgagttcga gtcaccaaag aagaagcgga aagtctcctc agagactggg
      301 cctgtcgccg tcgatccaac cctgcgccgc cggattgaac ctcacgagtt tgaagtgttc
      361 tttgaccccc gggagctgag aaaggagaca tgcctgctgt acgagatcaa ctggggaggc
      421 aggcactcca tctggaggca cacctctcag aacacaaata agcacgtgga ggtgaacttc
      481 atcgagaagt ttaccacaga gcggtacttc tgccccaata ccagatgtag catcacatgg
      541 tttctgagct ggtccccttg cggagagtgt agcagggcca tcaccgagtt cctgtccaga
      601 tatccacacg tgacactgtt tatctacatc gccaggctgt atcaccacgc agacccaagg
      661 aataggcagg gcctgcgcga tctgatcagc tccggcgtga ccatccagat catgacagag
      721 caggagtccg gctactgctg gcggaacttc gtgaattatt ctcctagcaa cgaggcccac
      781 tggcctaggt acccacacct gtgggtgcgc ctgtacgtgc tggagctgta ttgcatcatc
      841 ctgggcctgc ccccttgtct gaatatcctg cggagaaagc agccccagct gaccttcttt
      901 acaatcgccc tgcagtcttg tcactatcag aggctgccac cccacatcct gtgggccaca
      961 ggcctgaagt ctggaggatc tagcggagga tcctctggca gcgagacacc aggaacaagc
     1021 gagtcagcaa caccagagag cagtggcggc agcagcggcg gcagcgacaa gaagtacagc
     1081 atcggcctgg ccatcggcac caactctgtg ggctgggccg tgatcaccga cgagtacaag
     1141 gtgcccagca agaaattcaa ggtgctgggc aacaccgacc ggcacagcat caagaagaac
     1201 ctgatcggag ccctgctgtt cgacagcggc gaaacagccg agagaacccg gctgaagaga
     1261 accgccagaa gaagatacac cagacggaag aaccggatct gctatctgca agagatcttc
     1321 agcaacgaga tggccaaggt ggacgacagc ttcttccaca gactggaaga gtccttcctg
     1381 gtggaagagg ataagaagca cgagcggcac cccatcttcg gcaacatcgt ggacgaggtg
     1441 gcctaccacg agaagtaccc caccatctac cacctgagaa agaaactggt ggacagcacc
     1501 gacaaggccg acctgcggct gatctatctg gccctggccc acatgatcaa gttccggggc
     1561 cacttcctga tcgagggcga cctgaacccc gacaacagcg acgtggacaa gctgttcatc
     1621 cagctggtgc agacctacaa ccagctgttc gaggaaaacc ccatcaacgc cagcggcgtg
     1681 gacgccaagg ccatcctgtc tgccagactg agcaagagca gacggctgga aaatctgatc
     1741 gcccagctgc ccggcgagaa gaagaatggc ctgttcggaa acctgattgc cctgagcctg
     1801 ggcctgaccc ccaacttcaa gagcaacttc gacctggccg aggatgccaa actgcagctg
     1861 agcaaggaca cctacgacga cgacctggac aacctgctgg cccagatcgg cgaccagtac
     1921 gccgacctgt ttctggccgc caagaacctg tccgacgcca tcctgctgag cgacatcctg
     1981 agagtgaaca ccgagatcac caaggccccc ctgagcgcct ctatgatcaa gagatacgac
     2041 gagcaccacc aggacctgac cctgctgaaa gctctcgtgc ggcagcagct gcctgagaag
     2101 tacaaagaga ttttcttcga ccagagcaag aacggctacg ccggctacat tgacggcgga
     2161 gccagccagg aagagttcta caagttcatc aagcccatcc tggaaaagat ggacggcacc
     2221 gaggaactgc tcgtgaagct gaacagagag gacctgctgc ggaagcagcg gaccttcgac
     2281 aacggcagca tcccccacca gatccacctg ggagagctgc acgccattct gcggcggcag
     2341 gaagattttt acccattcct gaaggacaac cgggaaaaga tcgagaagat cctgaccttc
     2401 cgcatcccct actacgtggg ccctctggcc aggggaaaca gcagattcgc ctggatgacc
     2461 agaaagagcg aggaaaccat caccccctgg aacttcgagg aagtggtgga caagggcgct
     2521 tccgcccaga gcttcatcga gcggatgacc aacttcgata agaacctgcc caacgagaag
     2581 gtgctgccca agcacagcct gctgtacgag tacttcaccg tgtataacga gctgaccaaa
     2641 gtgaaatacg tgaccgaggg aatgagaaag cccgccttcc tgagcggcga gcagaaaaag
     2701 gccatcgtgg acctgctgtt caagaccaac cggaaagtga ccgtgaagca gctgaaagag
     2761 gactacttca agaaaatcga gtgcttcgac tccgtggaaa tctccggcgt ggaagatcgg
     2821 ttcaacgcct ccctgggcac ataccacgat ctgctgaaaa ttatcaagga caaggacttc
     2881 ctggacaatg aggaaaacga ggacattctg gaagatatcg tgctgaccct gacactgttt
     2941 gaggacagag agatgatcga ggaacggctg aaaacctatg cccacctgtt cgacgacaaa
     3001 gtgatgaagc agctgaagcg gcggagatac accggctggg gcaggctgag ccggaagctg
     3061 atcaacggca tccgggacaa gcagtccggc aagacaatcc tggatttcct gaagtccgac
     3121 ggcttcgcca acagaaactt catgcagctg atccacgacg acagcctgac ctttaaagag
     3181 gacatccaga aagcccaggt gtccggccag ggcgatagcc tgcacgagca cattgccaat
     3241 ctggccggca gccccgccat taagaagggc atcctgcaga cagtgaaggt ggtggacgag
     3301 ctcgtgaaag tgatgggccg gcacaagccc gagaacatcg tgatcgaaat ggccagagag
     3361 aaccagacca cccagaaggg acagaagaac agccgcgaga gaatgaagcg gatcgaagag
     3421 ggcatcaaag agctgggcag ccagatcctg aaagaacacc ccgtggaaaa cacccagctg
     3481 cagaacgaga agctgtacct gtactacctg cagaatgggc gggatatgta cgtggaccag
     3541 gaactggaca tcaaccggct gtccgactac gatgtggacc atatcgtgcc tcagagcttt
     3601 ctgaaggacg actccatcga caacaaggtg ctgaccagaa gcgacaagaa ccggggcaag
     3661 agcgacaacg tgccctccga agaggtcgtg aagaagatga agaactactg gcggcagctg
     3721 ctgaacgcca agctgattac ccagagaaag ttcgacaatc tgaccaaggc cgagagaggc
     3781 ggcctgagcg aactggataa ggccggcttc atcaagagac agctggtgga aacccggcag
     3841 atcacaaagc acgtggcaca gatcctggac tcccggatga acactaagta cgacgagaat
     3901 gacaagctga tccgggaagt gaaagtgatc accctgaagt ccaagctggt gtccgatttc
     3961 cggaaggatt tccagtttta caaagtgcgc gagatcaaca actaccacca cgcccacgac
     4021 gcctacctga acgccgtcgt gggaaccgcc ctgatcaaaa agtaccctaa gctggaaagc
     4081 gagttcgtgt acggcgacta caaggtgtac gacgtgcgga agatgatcgc caagagcgag
     4141 caggaaatcg gcaaggctac cgccaagtac ttcttctaca gcaacatcat gaactttttc
     4201 aagaccgaga ttaccctggc caacggcgag atccggaagc ggcctctgat cgagacaaac
     4261 ggcgaaaccg gggagatcgt gtgggataag ggccgggatt ttgccaccgt gcggaaagtg
     4321 ctgagcatgc cccaagtgaa tatcgtgaaa aagaccgagg tgcagacagg cggcttcagc
     4381 aaagagtcta tcagacccaa gaggaacagc gataagctga tcgccagaaa gaaggactgg
     4441 gaccctaaga agtacggcgg cttcctgtgg cccaccgtgg cctattctgt gctggtggtg
     4501 gccaaagtgg aaaagggcaa gtccaagaaa ctgaagagtg tgaaagagct gctggggatc
     4561 accatcatgg aaagaagcag cttcgagaag aatcccatcg actttctgga agccaagggc
     4621 tacaaagaag tgaaaaagga cctgatcatc aagctgccta agtactccct gttcgagctg
     4681 gaaaacggcc ggaagagaat gctggcctct gccaagcagc tgcagaaggg aaacgaactg
     4741 gccctgccct ccaaatatgt gaacttcctg tacctggcca gccactatga gaagctgaag
     4801 ggctcccccg aggataatga gcagaaacag ctgtttgtgg aacagcacaa gcactacctg
     4861 gacgagatca tcgagcagat cagcgagttc tccaagagag tgatcctggc cgacgctaat
     4921 ctggacaaag tgctgtccgc ctacaacaag caccgggata agcccatcag agagcaggcc
     4981 gagaatatca tccacctgtt taccctgacc agactgggag cccctagagc cttcaagtac
     5041 tttgacacca ccatcgaccc caagcagtac agaagcacca aagaggtgct ggacgccacc
     5101 ctgatccacc agagcatcac cggcctgtac gagacacgga tcgacctgtc tcagctggga
     5161 ggtgacagcg gcgggagcgg cgggagcggg gggagcacta atctgagcga catcattgag
     5221 aaggagactg ggaaacagct ggtcattcag gagtccatcc tgatgctgcc tgaggaggtg
     5281 gaggaagtga tcggcaacaa gccagagtct gacatcctgg tgcacaccgc ctacgacgag
     5341 tccacagatg agaatgtgat gctgctgacc tctgacgccc ccgagtataa gccttgggcc
     5401 ctggtcatcc aggattctaa cggcgagaat aagatcaaga tgctgagcgg aggatccgga
     5461 ggatctggag gcagcaccaa cctgtctgac atcatcgaga aggagacagg caagcagctg
     5521 gtcatccagg agagcatcct gatgctgccc gaagaagtcg aagaagtgat cggaaacaag
     5581 cctgagagcg atatcctggt ccataccgcc tacgacgaga gtaccgacga aaatgtgatg
     5641 ctgctgacat ccgacgcccc agagtataag ccctgggctc tggtcatcca ggattccaac
     5701 ggagagaaca aaatcaaaat gctgtctggc ggctcaaaaa gaaccgccga cggcagcgaa
     5761 ttcgagccca agaagaagag gaaagtcgga ggcggttgaa tcgctgcctt ctgcggggct
     5821 tgccttctgg ccatgccctt cttctctccc ttgcacctgt acctcttggt ctttgaataa
     5881 agcctgagta ggaagtgagg gtctagaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     5941 aaaaaaaaaa aaaaaaaaaa atgagacgcg atcaataacc gacactcctg cgtcgcctac
     6001 gcgctacgat ctgcagaatt ccagcacact ggcggccgtt actagtggat ccgagctcgg
     6061 taccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     6121 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     6181 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     6241 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     6301 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     6361 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     6421 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     6481 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     6541 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     6601 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     6661 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     6721 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     6781 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     6841 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     6901 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     6961 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     7021 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     7081 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     7141 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     7201 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     7261 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     7321 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     7381 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     7441 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     7501 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     7561 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     7621 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     7681 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     7741 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     7801 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     7861 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     7921 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     7981 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     8041 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     8101 tcatactctt cctttttcaa ttcagaagaa ctcgtcaaga aggcgataga aggcgatgcg
     8161 ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc
     8221 aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc
     8281 cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa
     8341 gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct
     8401 ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat cctgatcgac
     8461 aagaccggct tccatccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa
     8521 tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac
     8581 tttctcggca ggagcaaggt gggatgacag gagatcctgc cccggcactt cgcccaatag
     8641 cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt
     8701 cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag
     8761 gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc
     8821 agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc
     8881 cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc
     8941 ttgatcagat cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt
     9001 tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct
     9061 tgctgtccat aaaaccgccc agtctagcta tcgccatgta agcccactgc aagctacctg
     9121 ctttctcttt gcgcttgcgt tttcccttgt ccagatagcc cagtagctga cattcatccg
     9181 gggtcagcac cgtttctgcg gactggcttt ctacgtgttc cgcttccttt agcagccctt
     9241 gcgccctgaa ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac
     9301 caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg
     9361 agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa
     9421 gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt
     9481 tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt
     9541 agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga
     9601 gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc
     9661 gcgcttaatg cgccgctaca gggcgcgtcc attcgccatt caggctgcgc aactgttggg
     9721 aagggcgatc ggtgcgggcc tcttcgctat tacgccagct ggcgaaaggg ggatgtg
//
