LOCUS       PD0054_pIVTRup(original)-mCherry2        4913 bp    DNA     circular UNA 30-JAN-2020
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...6m-bsz22pg
VERSION     urn.local...6m-bsz22pg
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     2..21
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="143213387850957dbec548-2070-4b4b-9021-9
                     cd0e72277c8"
                     /%GC="50.0"
                     /Tm="58.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGCAAGGCGATTAAGTTGGG"
                     /label="PCR_0128"
     promoter        75..93
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     145..205
                     /Sequence="TTGGACCCTCGTACAGAAGCTAATACGACTCACTATAGGGAAATAAG
                     AGAGAAAAGAAGAG"
                     /Mismatches=0
                     /annotation_group="899387c9-8bde-4dd0-9324-a7d2555c062d"
                     /label="pIVTRup-BsmBI-FWD"
     promoter        165..183
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     misc_feature    232..942
                     /Source="referenceToDocument: urn:local:.:11h-bsz22tq"
                     /label="PCR_mCherry2 (concatenated sequence 2)"
                     /note="Geneious type: Concatenated sequence"
     primer_bind     232..252
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="143207634520682d857920-4993-4c5f-806a-1
                     fe95b0b3133"
                     /%GC="61.9"
                     /Tm="64.5"
                     /Hairpin_Tm="47.5"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATGGTGAGCAAGGGCGAGGAG"
                     /label="PCR_0058"
     primer_bind     232..252
                     /Mismatches=0
                     /Extension="GGATCCGGAGCCACGAACTTCTCTCTGTTAAAGCAAGCAGGCGACG
                     TGGAAGAAAACCCCGGTCCT"
                     /created_by="primer3"
                     /annotation_group="14320804197446b876a04d-2cc7-4850-adb2-f
                     b9f8e0f09fc"
                     /%GC="61.9"
                     /Tm="64.5"
                     /Hairpin_Tm="47.5"
                     /Self_Dimer_Tm="None"
                     /Sequence="ATGGTGAGCAAGGGCGAGGAG"
                     /label="PCR_0062"
     misc_feature    complement(284..303)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14321576368277e6fe844f-daeb-4e2e-b735-3
                     7426b9b6f19"
                     /%GC="55.0"
                     /Tm="59.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="15.7"
                     /Sequence="CTCCATGTGCACCTTGAACC"
                     /label="PCR_0316"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(377..395)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14321299661265f62ad7fb-5e05-4b1c-a49c-1
                     af0ff450408"
                     /%GC="52.6"
                     /Tm="57.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TTGGTCACCTTCAGCTTGG"
                     /label="PCR_0124"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(377..395)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14321616959885c0b5517b-0f99-4f95-a639-7
                     89c83fcac98"
                     /%GC="52.6"
                     /Tm="57.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TTGGTCACCTTCAGCTTGG"
                     /label="PCR_0317"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     526..547
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14321539155968e37a06c6-40f3-49bf-bcef-8
                     670b2fb5121"
                     /%GC="54.5"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GAGAGGGTGATGAACTTCGAGG"
                     /label="PCR_0244"
     primer_bind     533..552
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14321458509690d3d225a5-1a04-42b4-b8e8-c
                     46277f9390c"
                     /%GC="55.0"
                     /Tm="60.1"
                     /Hairpin_Tm="40.2"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGATGAACTTCGAGGACGGC"
                     /label="PCR_0190"
     primer_bind     823..844
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="143209698568692481b606-f701-403f-927d-4
                     6c28205a2e3"
                     /%GC="50.0"
                     /Tm="60.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TACAACGTGGACATCAAGCTGG"
                     /label="PCR_0085"
     misc_feature    complement(919..942)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14320719500329cb408daf-1bc1-42ee-a6ff-0
                     4ac49a0a0ed"
                     /%GC="54.2"
                     /Tm="64.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TCACTTGTACAGCTCGTCCATGCC"
                     /label="PCR_0054"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    943..231
                     /Source="referenceToDocument: urn:local:.:11g-bsz22tq"
                     /label="101362 - pIVTRup - EcoRV (concatenated sequence
                     1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    complement(1034..>1049)
                     /Sequence="TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
                     TTTTTTTTCTAGACCCTCACTTCC"
                     /Mismatches=0
                     /annotation_group="4d4b2582-45c5-4cb8-bab8-88be818af102"
                     /label="pIVTRup-BsmBI-REV"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    <1050..>1104
                     /Source="referenceToDocument: urn:local:.:11g-bsz22tq"
                     /label="101362 - pIVTRup - EcoRV (concatenated sequence
                     1)"
                     /note="Geneious type: Concatenated sequence"
     3'UTR           1050..1104
                     /created_by="User"
                     /label="polyA(55)"
     misc_feature    <1050..>1104
                     /Source="referenceToDocument: urn:local:.:11i-bsz22tq"
                     /label="pIVTRup-linearized (concatenated sequence 1)"
                     /note="Geneious type: Concatenated sequence"
     misc_feature    1104^1105
                     /Original_Bases="AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
                     AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
                     AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
                     AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
                     AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
                     AAAAAAAAAAAAAAAAAAAAAAAAAA"
                     /label="AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
                     AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
                     AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
                     AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
                     AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
                     AAAAAAAAAAAAAAAAA"
                     /note="Geneious type: Editing History Deletion"
     protein_bind    1243..1259
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     1261..1281
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="143214990992178a241872-acfa-4087-9e34-c
                     ca00d18d9a1"
                     /%GC="57.1"
                     /Tm="62.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="TCCACACAACATACGAGCCGG"
                     /label="PCR_0227"
     primer_bind     1262..1279
                     /Mismatches=0
                     /Extension="ACTGGCGAACTACTTACTCTAGCTTCCCGG"
                     /created_by="primer3"
                     /annotation_group="14321053518642c3e65e8e-10d0-4b55-99b0-e
                     52beda7657b"
                     /%GC="55.6"
                     /Tm="55.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCACACAACATACGAGCC"
                     /label="PCR_0096"
     primer_bind     1262..1279
                     /Mismatches=0
                     /Extension="TGTGGTGATGTTGAAGAAAACCCTGGTCCA"
                     /created_by="primer3"
                     /annotation_group="14321132146442d0690f7f-515b-4cb3-add5-3
                     1c057b0b32c"
                     /%GC="55.6"
                     /Tm="55.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCACACAACATACGAGCC"
                     /label="PCR_0099"
     promoter        complement(1267..1297)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     misc_feature    complement(1284..1304)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14321256205003809d2b1f-d8b8-4a17-8cb6-6
                     0c5f0d711bf"
                     /%GC="47.6"
                     /Tm="57.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCCAGGCTTTACACTTTATGC"
                     /label="PCR_0122"
                     /note="Geneious type: primer_bind_reverse"
     protein_bind    1312..1333
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     misc_feature    complement(1446..1465)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14320924902828fdf70530-23b7-45ff-8e64-0
                     cb15e430b84"
                     /%GC="60.0"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="9.1"
                     /Sequence="CGAGTCAGTGAGCGAGGAAG"
                     /label="PCR_0083"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1446..1465)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14321417082783d2b3a2ac-6dfc-4eec-888c-2
                     472916c57c6"
                     /%GC="60.0"
                     /Tm="60.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="9.1"
                     /Sequence="CGAGTCAGTGAGCGAGGAAG"
                     /label="PCR_0179"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1549..1569)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14321174354946255c0d7c-fb48-42d9-8e62-f
                     d1dac393b99"
                     /%GC="52.4"
                     /Tm="60.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="GCTCACATGTTCTTTCCTGCG"
                     /label="PCR_0101"
                     /note="Geneious type: primer_bind_reverse"
     rep_origin      complement(1621..2209)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     misc_feature    complement(1661..1680)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14321378629155bb7f1a23-f277-4968-93f5-3
                     f93953a2b79"
                     /%GC="60.0"
                     /Tm="60.7"
                     /Hairpin_Tm="36.7"
                     /Self_Dimer_Tm="None"
                     /Sequence="CACCTCTGACTTGAGCGTCG"
                     /label="PCR_0143"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(1948..1967)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14321661652648ee700da7-e2a1-40e9-aaf8-4
                     53815e47474"
                     /%GC="55.0"
                     /Tm="60.1"
                     /Hairpin_Tm="49.1"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCAGTGGCGATAAGTCGTGT"
                     /label="PCR_0340"
                     /note="Geneious type: primer_bind_reverse"
     CDS             complement(2380..3240)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="confers resistance to ampicillin, carbenicillin,
                     and related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
                     /label="AmpR"
     misc_feature    complement(2607..2634)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="1432068228400532dfe44b-e7c9-4e97-ba7a-1
                     d5644b887ca"
                     /%GC="46.4"
                     /Tm="63.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAACAATTAATAGACTGGATGGAGGCGG"
                     /label="PCR_0020"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2607..2634)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14320885618463d3e02f6d-93e3-482d-a67f-e
                     36a8c8c85db"
                     /%GC="46.4"
                     /Tm="63.8"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAACAATTAATAGACTGGATGGAGGCGG"
                     /label="PCR_0068"
                     /note="Geneious type: primer_bind_reverse"
     misc_feature    complement(2608..2634)
                     /Mismatches=0
                     /Extension="GGCGAAAGGGGGATGTGCTGCAAGGCGATT"
                     /created_by="primer3"
                     /annotation_group="14321091591139eb9b96d5-c8ca-4084-80ff-8
                     5f1a0c8bd87"
                     /%GC="44.4"
                     /Tm="62.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CAACAATTAATAGACTGGATGGAGGCG"
                     /label="PCR_0097"
                     /note="Geneious type: primer_bind_reverse"
     primer_bind     2635..2663
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14320641821142043430b5-5aae-4111-b928-6
                     6cf100fc23b"
                     /%GC="55.2"
                     /Tm="67.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCGGGAAGCTAGAGTAAGTAGTTCGCCAG"
                     /label="PCR_0015"
     primer_bind     2635..2663
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="14320846979868041c1638-5b07-48cc-b9de-0
                     ce8d362e45a"
                     /%GC="55.2"
                     /Tm="67.7"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="CCGGGAAGCTAGAGTAAGTAGTTCGCCAG"
                     /label="PCR_0067"
     CDS             complement(3258..4052)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /note="confers resistance to neomycin, kanamycin, and G418
                      (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL
                     LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY
                     QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
                     /label="NeoR/KanR"
     rep_origin      complement(4396..4824)
                     /direction="LEFT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     4753..4771
                     /modified_by="User"
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="1432121454454851efe447-50dc-4c18-b49e-0
                     28eda26f955"
                     /%GC="57.9"
                     /Tm="60.3"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Sequence="TGGCAAGTGTAGCGGTCAC"
                     /label="PCR_0114"
     misc_feature    complement(4906..13)
                     /Mismatches=0
                     /created_by="primer3"
                     /annotation_group="143210136093962b42109c-e74a-4261-a7d8-4
                     c88565f6990"
                     /%GC="57.1"
                     /Tm="63.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Sequence="AATCGCCTTGCAGCACATCCC"
                     /label="PCR_0093"
                     /note="Geneious type: primer_bind_reverse"
ORIGIN      
        1 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
       61 cggccagtga attgtaatac gactcactat agggcgaatt gggccctcta gatgcatgct
      121 cgagcggccg ccagtgtgat ggatttggac cctcgtacag aagctaatac gactcactat
      181 agggaaataa gagagaaaag aagagtaaga agaaatataa gagccaccga tatggtgagc
      241 aagggcgagg aggacaacat ggccatcatc aaggagttca tgaggttcaa ggtgcacatg
      301 gagggcagcg tgaacggcca cgagttcgag atcgagggcg agggcgaggg caggccctac
      361 gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc cttcgcctgg
      421 gacatcctga gcccccagtt catgtacggc agcaaggcct acgtgaagca ccccgccgac
      481 atccccgact acctgaagct gagcttcccc gagggcttca actgggagag ggtgatgaac
      541 ttcgaggacg gcggcgtggt gaccgtgacc caggacagca gcctgcagga cggcgagttc
      601 atctacaagg tgaagctgag gggcaccaac ttccccagcg acggccccgt gatgcagtgc
      661 aggaccatgg gctgggaggc cagcaccgag aggatgtacc ccgaggacgg cgccctgaag
      721 ggcgagatca agcagaggct gaagctgaag gacggcggcc actacgacgc cgaggtgaag
      781 accacctaca aggccaagaa gcccgtgcag ctgcccggcg cctacaacgt ggacatcaag
      841 ctggacatcc tgagccacaa cgaggactac accatcgtgg agcagtacga gagggccgag
      901 ggcaggcaca gcaccggcgg catggacgag ctgtacaagt gaatcgctgc cttctgcggg
      961 gcttgccttc tggccatgcc cttcttctct cccttgcacc tgtacctctt ggtctttgaa
     1021 taaagcctga gtaggaagtg agggtctaga aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa
     1081 aaaaaaaaaa aaaaaaaaaa aaaacgatca ataaccgaca ctcctgcgtc gcctacgcgc
     1141 tacgatctgc agaattccag cacactggcg gccgttacta gtggatccga gctcggtacc
     1201 aagcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat
     1261 tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag
     1321 ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg
     1381 ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
     1441 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     1501 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     1561 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     1621 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     1681 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     1741 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     1801 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     1861 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     1921 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     1981 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     2041 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     2101 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     2161 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     2221 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     2281 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     2341 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     2401 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     2461 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     2521 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     2581 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     2641 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     2701 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     2761 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     2821 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     2881 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     2941 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     3001 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     3061 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     3121 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     3181 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     3241 actcttcctt tttcaattca gaagaactcg tcaagaaggc gatagaaggc gatgcgctgc
     3301 gaatcgggag cggcgatacc gtaaagcacg aggaagcggt cagcccattc gccgccaagc
     3361 tcttcagcaa tatcacgggt agccaacgct atgtcctgat agcggtccgc cacacccagc
     3421 cggccacagt cgatgaatcc agaaaagcgg ccattttcca ccatgatatt cggcaagcag
     3481 gcatcgccat gggtcacgac gagatcctcg ccgtcgggca tgcgcgcctt gagcctggcg
     3541 aacagttcgg ctggcgcgag cccctgatgc tcttcgtcca gatcatcctg atcgacaaga
     3601 ccggcttcca tccgagtacg tgctcgctcg atgcgatgtt tcgcttggtg gtcgaatggg
     3661 caggtagccg gatcaagcgt atgcagccgc cgcattgcat cagccatgat ggatactttc
     3721 tcggcaggag caaggtggga tgacaggaga tcctgccccg gcacttcgcc caatagcagc
     3781 cagtcccttc ccgcttcagt gacaacgtcg agcacagctg cgcaaggaac gcccgtcgtg
     3841 gccagccacg atagccgcgc tgcctcgtcc tgcagttcat tcagggcacc ggacaggtcg
     3901 gtcttgacaa aaagaaccgg gcgcccctgc gctgacagcc ggaacacggc ggcatcagag
     3961 cagccgattg tctgttgtgc ccagtcatag ccgaatagcc tctccaccca agcggccgga
     4021 gaacctgcgt gcaatccatc ttgttcaatc atgcgaaacg atcctcatcc tgtctcttga
     4081 tcagatcttg atcccctgcg ccatcagatc cttggcggca agaaagccat ccagtttact
     4141 ttgcagggct tcccaacctt accagagggc gccccagctg gcaattccgg ttcgcttgct
     4201 gtccataaaa ccgcccagtc tagctatcgc catgtaagcc cactgcaagc tacctgcttt
     4261 ctctttgcgc ttgcgttttc ccttgtccag atagcccagt agctgacatt catccggggt
     4321 cagcaccgtt tctgcggact ggctttctac gtgttccgct tcctttagca gcccttgcgc
     4381 cctgaatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt tttaaccaat
     4441 aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata gggttgagtg
     4501 ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac gtcaaagggc
     4561 gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa tcaagttttt
     4621 tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc cgatttagag
     4681 cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg
     4741 gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc
     4801 ttaatgcgcc gctacagggc gcgtccattc gccattcagg ctgcgcaact gttgggaagg
     4861 gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat gtg
//
