LOCUS       pEP0016_pIVTRup(BsmBI)_BSD        4605 bp    DNA     circular UNA 18-OCT-2021
DEFINITION  Concatenation of 2 sequences.
ACCESSION   urn.local...n-bvs72m4
VERSION     urn.local...n-bvs72m4
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
FEATURES             Location/Qualifiers
     primer_bind     91..111
                     /Mismatches=0
                     /%GC="52.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="329937617469007270cf1f-87cd-4642-9f27-d
                     4e4ee46533e"
                     /Sequence="CTGCAAGGCGATTAAGTTGGG"
                     /created_by="primer3"
                     /label="PCR_0346"
     primer_bind     91..111
                     /Mismatches=0
                     /%GC="52.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.9"
                     /annotation_group="329947514978003ade0590-0a68-4b96-aa8e-4
                     7a56f8a2fbe"
                     /Sequence="CTGCAAGGCGATTAAGTTGGG"
                     /created_by="primer3"
                     /label="PCR_0353"
     primer_bind     92..111
                     /Mismatches=0
                     /%GC="50.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="58.8"
                     /annotation_group="3299370381580048b2314a-4cbd-4d92-bbc9-a
                     c5b98c4731d"
                     /Sequence="TGCAAGGCGATTAAGTTGGG"
                     /created_by="primer3"
                     /label="PCR_0128"
     promoter        165..183
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     primer_bind     176..194
                     /Mismatches=0
                     /%GC="52.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="5.4"
                     /Tm="57.0"
                     /annotation_group="329944627845007578bdd0-2e8c-4e75-a575-a
                     16f2feb52c7"
                     /Sequence="ACTATAGGGCGAATTGGGC"
                     /created_by="primer3"
                     /label="pIVTRup_BsmBI_insert_FWD"
     primer_bind     176..194
                     /Mismatches=0
                     /%GC="52.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="5.4"
                     /Tm="57.0"
                     /annotation_group="32994520089500615d9849-ad73-4656-903b-5
                     966c8594d2e"
                     /Sequence="ACTATAGGGCGAATTGGGC"
                     /created_by="primer3"
                     /label="pIVTRup_BsmBI_insert_FWD"
     promoter        255..273
                     /note="promoter for bacteriophage T7 RNA polymerase"
                     /label="T7 promoter"
     cds             272..>301
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="101362 - pIVTRup Copy"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="101362 - pIVTRup Copy, 101362 - pIVTRup
                     PCR DraI, Digestion of 101362 - pIVTRup PCR DraI -
                     Fragment 3"
                     /Transferred_Similarity="100.00%"
                     /label="3'UTR"
     misc_feature    301^302
                     /Original_Bases="GAAATATAAGAGCCACCGAT"
                     /label="GAAATATAAGAGCCACCGAT"
                     /note="Geneious type: Editing History Deletion"
     misc_feature    319..717
                     /Original_Bases="GATATGGACAAGAAGTACTCCATTGGGCTCGCTATCGGCAC
                     AAACAGCGTCGGCTGGGCCGTCATTACGGACGAGTACAAGGTGCCGAGCAAAAAATTC
                     AAAGTTCTGGGCAATACCGATCGCCACAGCATAAAGAAGAACCTCATTGGCGCCCTCC
                     TGTTCGACTCCGGGGAGACGGCCGAAGCCACGCGGCTCAAAAGAACAGCACGGCGCAG
                     ATATACCCGCAGAAAGAATCGGATCTGCTACCTGCAGGAGATCTTTAGTAATGAGATG
                     GCTAAGGTGGATGACTCTTTCTTCCATAGGCTGGAGGAGTCCTTTTTGGTGGAGGAGG
                     ATAAAAAGCACGAGCGCCACCCAATCTTTGGCAATATCGTGGACGAGGTGGCGTACCA
                     TGAAAAGTACCCAACCATATATCATCTGAGGAAGAAGCTTGTAGACAGTACTGATAAG
                     GCTGACTTGCGGTTGATCTATCTCGCGCTGGCGCATATGATCAAATTTCGGGGACACT
                     TCCTCATCGAGGGGGACCTGAACCCAGACAACAGCGATGTCGATAAACTCTTTATCCA
                     ACTGGTTCAGACTTACAATCAGCTTTTCGAAGAGAACCCGATCAACGCATCCGGAGTT
                     GACGCCAAAGCAATCCTGAGCGCTAGGCTGTCCAAATCCCGGCGGCTCGAAAACCTCA
                     TCGCACAGCTCCCTGGGGAGAAGAAGAACGGCCTGTTTGGTAATCTTATCGCCCTGTC
                     ACTCGGGCTGACCCCCAACTTTAAATCTAACTTCGACCTGGCCGAAGATGCCAAGCTT
                     CAACTGAGCAAAGACACCTACGATGATGATCTCGACAATCTGCTGGCCCAGATCGGCG
                     ACCAGTACGCAGACCTTTTTTTGGCGGCAAAGAACCTGTCAGACGCCATTCTGCTGAG
                     TGATATTCTGCGAGTGAACACGGAGATCACCAAAGCTCCGCTGAGCGCTAGTATGATC
                     AAGCGCTATGATGAGCACCACCAAGACTTGACTTTGCTGAAGGCCCTTGTCAGACAGC
                     AACTGCCTGAGAAGTACAAGGAAATTTTCTTCGATCAGTCTAAAAATGGCTACGCCGG
                     ATACATTGACGGCGGAGCAAGCCAGGAGGAATTTTACAAATTTATTAAGCCCATCTTG
                     GAAAAAATGGACGGCACCGAGGAGCTGCTGGTAAAGCTTAACAGAGAAGATCTGTTGC
                     GCAAACAGCGCACTTTCGACAATGGAAGCATCCCCCACCAGATTCACCTGGGCGAACT
                     GCACGCTATCCTCAGGCGGCAAGAGGATTTCTACCCCTTTTTGAAAGATAACAGGGAA
                     AAGATTGAGAAAATCCTCACATTTCGGATACCCTACTATGTAGGCCCCCTCGCCCGGG
                     GAAATTCCAGATTCGCGTGGATGACTCGCAAATCAGAAGAGACCATCACTCCCTGGAA
                     CTTCGAGGAAGTCGTGGATAAGGGGGCCTCTGCCCAGTCCTTCATCGAAAGGATGACT
                     AACTTTGATAAAAATCTGCCTAACGAAAAGGTGCTTCCTAAACACTCTCTGCTGTACG
                     AGTACTTCACAGTTTATAACGAGCTCACCAAGGTCAAATACGTCACAGAAGGGATGAG
                     AAAGCCAGCATTCCTGTCTGGAGAGCAGAAGAAAGCTATCGTGGACCTCCTCTTCAAG
                     ACGAACCGGAAAGTTACCGTGAAACAGCTCAAAGAAGACTATTTCAAAAAGATTGAAT
                     GTTTCGACTCTGTTGAAATCAGCGGAGTGGAGGATCGCTTCAACGCATCCCTGGGAAC
                     GTATCACGATCTCCTGAAAATCATTAAAGACAAGGACTTCCTGGACAATGAGGAGAAC
                     GAGGACATTCTTGAGGACATTGTCCTCACCCTTACGTTGTTTGAAGATAGGGAGATGA
                     TTGAAGAACGCTTGAAAACTTACGCTCATCTCTTCGACGACAAAGTCATGAAACAGCT
                     CAAGAGGCGCCGATATACAGGATGGGGGCGGCTGTCAAGAAAACTGATCAATGGGATC
                     CGAGACAAGCAGAGTGGAAAGACAATCCTGGATTTTCTTAAGTCCGATGGATTTGCCA
                     ACCGGAACTTCATGCAGTTGATCCATGATGACTCTCTCACCTTTAAGGAGGACATCCA
                     GAAAGCACAAGTTTCTGGCCAGGGGGACAGTCTTCACGAGCACATCGCTAATCTTGCA
                     GGTAGCCCAGCTATCAAAAAGGGAATACTGCAGACCGTTAAGGTCGTGGATGAACTCG
                     TCAAAGTAATGGGAAGGCATAAGCCCGAGAATATCGTTATCGAGATGGCCCGAGAGAA
                     CCAAACTACCCAGAAGGGACAGAAGAACAGTAGGGAAAGGATGAAGAGGATTGAAGAG
                     GGTATAAAAGAACTGGGGTCCCAAATCCTTAAGGAACACCCAGTTGAAAACACCCAGC
                     TTCAGAATGAGAAGCTCTACCTGTACTACCTGCAGAACGGCAGGGACATGTACGTGGA
                     TCAGGAACTGGACATCAATCGGCTCTCCGACTACGACGTGGCTGCTATCGTGCCCCAG
                     TCTTTTCTCAAAGATGATTCTATTGATAATAAAGTGTTGACAAGATCCGATAAAGCTA
                     GAGGGAAGAGTGATAACGTCCCCTCAGAAGAAGTTGTCAAGAAAATGAAAAATTATTG
                     GCGGCAGCTGCTGAACGCCAAACTGATCACACAACGGAAGTTCGATAATCTGACTAAG
                     GCTGAACGAGGTGGCCTGTCTGAGTTGGATAAAGCCGGCTTCATCAAAAGGCAGCTTG
                     TTGAGACACGCCAGATCACCAAGCACGTGGCCCAAATTCTCGATTCACGCATGAACAC
                     CAAGTACGATGAAAATGACAAACTGATTCGAGAGGTGAAAGTTATTACTCTGAAGTCT
                     AAGCTGGTCTCAGATTTCAGAAAGGACTTTCAGTTTTATAAGGTGAGAGAGATCAACA
                     ATTACCACCATGCGCATGATGCCTACCTGAATGCAGTGGTAGGCACTGCACTTATCAA
                     AAAATATCCCAAGCTTGAATCTGAATTTGTTTACGGAGACTATAAAGTGTACGATGTT
                     AGGAAAATGATCGCAAAGTCTGAGCAGGAAATAGGCAAGGCCACCGCTAAGTACTTCT
                     TTTACAGCAATATTATGAATTTTTTCAAGACCGAGATTACACTGGCCAATGGAGAGAT
                     TCGGAAGCGACCACTTATCGAAACAAACGGAGAAACAGGAGAAATCGTGTGGGACAAG
                     GGTAGGGATTTCGCGACAGTCCGGAAGGTCCTGTCCATGCCGCAGGTGAACATCGTTA
                     AAAAGACCGAAGTACAGACCGGAGGCTTCTCCAAGGAAAGTATCCTCCCGAAAAGGAA
                     CAGCGACAAGCTGATCGCACGCAAAAAAGATTGGGACCCCAAGAAATACGGCGGATTC
                     GATTCTCCTACAGTCGCTTACAGTGTACTGGTTGTGGCCAAAGTGGAGAAAGGGAAGT
                     CTAAAAAACTCAAAAGCGTCAAGGAACTGCTGGGCATCACAATCATGGAGCGATCAAG
                     CTTCGAAAAAAACCCCATCGACTTTCTCGAGGCGAAAGGATATAAAGAGGTCAAAAAA
                     GACCTCATCATTAAGCTTCCCAAGTACTCTCTCTTTGAGCTTGAAAACGGCCGGAAAC
                     GAATGCTCGCTAGTGCGGGCGAGCTGCAGAAAGGTAACGAGCTGGCACTGCCCTCTAA
                     ATACGTTAATTTCTTGTATCTGGCCAGCCACTATGAAAAGCTCAAAGGGTCTCCCGAA
                     GATAATGAGCAGAAGCAGCTGTTCGTGGAACAACACAAACACTACCTTGATGAGATCA
                     TCGAGCAAATAAGCGAATTCTCCAAAAGAGTGATCCTCGCCGACGCTAACCTCGATAA
                     GGTGCTTTCTGCTTACAATAAGCACAGGGATAAGCCCATCAGGGAGCAGGCAGAAAAC
                     ATTATCCACTTGTTTACTCTGACCAACTTGGGCGCGCCTGCAGCCTTCAAGTACTTCG
                     ACACCACCATAGACAGAAAGCGGTACACCTCTACAAAGGAGGTCCTGGACGCCACACT
                     GATTCATCAGTCAATTACGGGGCTCTATGAAACAAGAATCGACCTCTCTCAGCTCGGT
                     GGAGACAGCAGGGCTGACCCCAAGAAGAAGAGGAAGGTGAGTGGTGGAGGAAGTGGCG
                     GGTCAGGGTCGATGGACGCGAAATCACTTACGGCATGGTCGAGAACACTGGTTACGTT
                     CAAGGACGTGTTTGTGGACTTTACACGTGAGGAGTGGAAATTGCTGGATACTGCGCAA
                     CAAATTGTGTATCGAAATGTCATGCTTGAGAATTACAAGAACCTCGTCAGTCTCGGAT
                     ACCAGTTGACGAAACCGGATGTGATCCTTAGGCTCGAAAAGGGGGAAGAACCTTGGCT
                     GGTATCGGGAGGTGGTTCGGGTGGCTCTGGATCAAGCCCAAAGAAGAAACGGAAGGTG
                     GAAGCCTCAGTGCAGGTGAAAAGGGTGCTGGAAAAATCCCCCGGCAAACTCCTCGTGA
                     AGATGCCCTTCCAGGCTTCCCCTGGCGGAAAAGGTGAAGGGGGTGGCGCAACCACATC
                     TGCCCAGGTCATGGTCATCAAGCGACCTGGAAGGAAAAGAAAGGCCGAGGCTGACCCT
                     CAGGCCATTCCAAAGAAACGGGGACGCAAGCCAGGGTCCGTGGTCGCAGCTGCAGCAG
                     CTGAGGCTAAGAAAAAGGCAGTGAAGGAAAGCTCCATCCGCAGTGTGCAGGAGACTGT
                     CCTGCCCATCAAGAAGAGGAAGACTAGGGAGACCGTGTCCATCGAGGTCAAAGAAGTG
                     GTCAAGCCCCTGCTCGTGTCCACCCTGGGCGAAAAATCTGGAAAGGGGCTCAAAACAT
                     GCAAGTCACCTGGACGGAAAAGCAAGGAGTCTAGTCCAAAGGGGCGCTCAAGCTCCGC
                     TTCTAGTCCCCCTAAAAAGGAACACCATCACCATCACCATCACGCCGAGTCTCCTAAG
                     GCTCCTATGCCACTGCTCCCACCACCTCCACCACCTGAGCCACAGTCAAGCGAAGACC
                     CCATCAGCCCACCCGAGCCTCAGGATCTGTCCTCTAGTATTTGCAAAGAGGAAAAGAT
                     GCCCAGAGCAGGCAGCCTGGAGAGTGATGGCTGTCCAAAAGAACCCGCCAAGACCCAG
                     CCTATGGTGGCAGCCGCTGCAACTACCACCACAACCACAACTACCACAGTGGCCGAAA
                     AATACAAGCATCGCGGCGAGGGCGAACGAAAGGACATTGTGTCAAGCTCCATGCCCAG
                     ACCTAACCGGGAGGAACCAGTCGATAGTAGGACACCCGTGACTGAGAGAGTCTCATAG
                     "
                     /label="GATATGGACAAGAAGTACTCCATTGGGCTCGCTATCGGCACAAACAGCGT
                     CGGCTGGGCCGTCATTACGGACGAGTACAAGGTGCCGAGCAAAAAATTCAAAGTTCTG
                     GGCAATACCGATCGCCACAGCATAAAGAAGAACCTCATTGGCGCCCTCCTGTTCGACT
                     CCGGGGAGACGGCCGAAGCCACGCGGCTCAAAAGAACAGCACGGCGCAGATATACCCG
                     CAGAAAGAATCGGATCTGCTACCTGCAGGAGATCTTTAGTAATGAGATGGCTAAGGTG
                     GATGACTCTTTCTTCCATAGGCTGGAGGAGTCCTTTTTGGTGGAGGAGGATAAAAAGC
                     ACGAGCGCCACCCAATCTTTGGCAATATCGTGGACGAGGTGGCGTACCATGAAAAGTA
                     CCCAACCATATATCATCTGAGGAAGAAGCTTGTAGACAGTACTGATAAGGCTGACTTG
                     CGGTTGATCTATCTCGCGCTGGCGCATATGATCAAATTTCGGGGACACTTCCTCATCG
                     AGGGGGACCTGAACCCAGACAACAGCGATGTCGATAAACTCTTTATCCAACTGGTTCA
                     GACTTACAATCAGCTTTTCGAAGAGAACCCGATCAACGCATCCGGAGTTGACGCCAAA
                     GCAATCCTGAGCGCTAGGCTGTCCAAATCCCGGCGGCTCGAAAACCTCATCGCACAGC
                     TCCCTGGGGAGAAGAAGAACGGCCTGTTTGGTAATCTTATCGCCCTGTCACTCGGGCT
                     GACCCCCAACTTTAAATCTAACTTCGACCTGGCCGAAGATGCCAAGCTTCAACTGAGC
                     AAAGACACCTACGATGATGATCTCGACAATCTGCTGGCCCAGATCGGCGACCAGTACG
                     CAGACCTTTTTTTGGCGGCAAAGAACCTGTCAGACGCCATTCTGCTGAGTGATATTCT
                     GCGAGTGAACACGGAGATCACCAAAGCTCCGCTGAGCGCTAGTATGATCAAGCGCTAT
                     GATGAGCACCACCAAGACTTGACTTTGCTGAAGGCCCTTGTCAGACAGCAACTGCCTG
                     AGAAGTACAAGGAAATTTTCTTCGATCAGTCTAAAAATGGCTACGCCGGATACATTGA
                     CGGCGGAGCAAGCCAGGAGGAATTTTACAAATTTATTAAGCCCATCTTGGAAAAAATG
                     GACGGCACCGAGGAGCTGCTGGTAAAGCTTAACAGAGAAGATCTGTTGCGCAAACAGC
                     GCACTTTCGACAATGGAAGCATCCCCCACCAGATTCACCTGGGCGAACTGCACGCTAT
                     CCTCAGGCGGCAAGAGGATTTCTACCCCTTTTTGAAAGATAACAGGGAAAAGATTGAG
                     AAAATCCTCACATTTCGGATACCCTACTATGTAGGCCCCCTCGCCCGGGGAAATTCCA
                     GATTCGCGTGGATGACTCGCAAATCAGAAGAGACCATCACTCCCTGGAACTTCGAGGA
                     AGTCGTGGATAAGGGGGCCTCTGCCCAGTCCTTCATCGAAAGGATGACTAACTTTGAT
                     AAAAATCTGCCTAACGAAAAGGTGCTTCCTAAACACTCTCTGCTGTACGAGTACTTCA
                     CAGTTTATAACGAGCTCACCAAGGTCAAATACGTCACAGAAGGGATGAGAAAGCCAGC
                     ATTCCTGTCTGGAGAGCAGAAGAAAGCTATCGTGGACCTCCTCTTCAAGACGAACCGG
                     AAAGTTACCGTGAAACAGCTCAAAGAAGACTATTTCAAAAAGATTGAATGTTTCGACT
                     CTGTTGAAATCAGCGGAGTGGAGGATCGCTTCAACGCATCCCTGGGAACGTATCACGA
                     TCTCCTGAAAATCATTAAAGACAAGGACTTCCTGGACAATGAGGAGAACGAGGACATT
                     CTTGAGGACATTGTCCTCACCCTTACGTTGTTTGAAGATAGGGAGATGATTGAAGAAC
                     GCTTGAAAACTTACGCTCATCTCTTCGACGACAAAGTCATGAAACAGCTCAAGAGGCG
                     CCGATATACAGGATGGGGGCGGCTGTCAAGAAAACTGATCAATGGGATCCGAGACAAG
                     CAGAGTGGAAAGACAATCCTGGATTTTCTTAAGTCCGATGGATTTGCCAACCGGAACT
                     TCATGCAGTTGATCCATGATGACTCTCTCACCTTTAAGGAGGACATCCAGAAAGCACA
                     AGTTTCTGGCCAGGGGGACAGTCTTCACGAGCACATCGCTAATCTTGCAGGTAGCCCA
                     GCTATCAAAAAGGGAATACTGCAGACCGTTAAGGTCGTGGATGAACTCGTCAAAGTAA
                     TGGGAAGGCATAAGCCCGAGAATATCGTTATCGAGATGGCCCGAGAGAACCAAACTAC
                     CCAGAAGGGACAGAAGAACAGTAGGGAAAGGATGAAGAGGATTGAAGAGGGTATAAAA
                     GAACTGGGGTCCCAAATCCTTAAGGAACACCCAGTTGAAAACACCCAGCTTCAGAATG
                     AGAAGCTCTACCTGTACTACCTGCAGAACGGCAGGGACATGTACGTGGATCAGGAACT
                     GGACATCAATCGGCTCTCCGACTACGACGTGGCTGCTATCGTGCCCCAGTCTTTTCTC
                     AAAGATGATTCTATTGATAATAAAGTGTTGACAAGATCCGATAAAGCTAGAGGGAAGA
                     GTGATAACGTCCCCTCAGAAGAAGTTGTCAAGAAAATGAAAAATTATTGGCGGCAGCT
                     GCTGAACGCCAAACTGATCACACAACGGAAGTTCGATAATCTGACTAAGGCTGAACGA
                     GGTGGCCTGTCTGAGTTGGATAAAGCCGGCTTCATCAAAAGGCAGCTTGTTGAGACAC
                     GCCAGATCACCAAGCACGTGGCCCAAATTCTCGATTCACGCATGAACACCAAGTACGA
                     TGAAAATGACAAACTGATTCGAGAGGTGAAAGTTATTACTCTGAAGTCTAAGCTGGTC
                     TCAGATTTCAGAAAGGACTTTCAGTTTTATAAGGTGAGAGAGATCAACAATTACCACC
                     ATGCGCATGATGCCTACCTGAATGCAGTGGTAGGCACTGCACTTATCAAAAAATATCC
                     CAAGCTTGAATCTGAATTTGTTTACGGAGACTATAAAGTGTACGATGTTAGGAAAATG
                     ATCGCAAAGTCTGAGCAGGAAATAGGCAAGGCCACCGCTAAGTACTTCTTTTACAGCA
                     ATATTATGAATTTTTTCAAGACCGAGATTACACTGGCCAATGGAGAGATTCGGAAGCG
                     ACCACTTATCGAAACAAACGGAGAAACAGGAGAAATCGTGTGGGACAAGGGTAGGGAT
                     TTCGCGACAGTCCGGAAGGTCCTGTCCATGCCGCAGGTGAACATCGTTAAAAAGACCG
                     AAGTACAGACCGGAGGCTTCTCCAAGGAAAGTATCCTCCCGAAAAGGAACAGCGACAA
                     GCTGATCGCACGCAAAAAAGATTGGGACCCCAAGAAATACGGCGGATTCGATTCTCCT
                     ACAGTCGCTTACAGTGTACTGGTTGTGGCCAAAGTGGAGAAAGGGAAGTCTAAAAAAC
                     TCAAAAGCGTCAAGGAACTGCTGGGCATCACAATCATGGAGCGATCAAGCTTCGAAAA
                     AAACCCCATCGACTTTCTCGAGGCGAAAGGATATAAAGAGGTCAAAAAAGACCTCATC
                     ATTAAGCTTCCCAAGTACTCTCTCTTTGAGCTTGAAAACGGCCGGAAACGAATGCTCG
                     CTAGTGCGGGCGAGCTGCAGAAAGGTAACGAGCTGGCACTGCCCTCTAAATACGTTAA
                     TTTCTTGTATCTGGCCAGCCACTATGAAAAGCTCAAAGGGTCTCCCGAAGATAATGAG
                     CAGAAGCAGCTGTTCGTGGAACAACACAAACACTACCTTGATGAGATCATCGAGCAAA
                     TAAGCGAATTCTCCAAAAGAGTGATCCTCGCCGACGCTAACCTCGATAAGGTGCTTTC
                     TGCTTACAATAAGCACAGGGATAAGCCCATCAGGGAGCAGGCAGAAAACATTATCCAC
                     TTGTTTACTCTGACCAACTTGGGCGCGCCTGCAGCCTTCAAGTACTTCGACACCACCA
                     TAGACAGAAAGCGGTACACCTCTACAAAGGAGGTCCTGGACGCCACACTGATTCATCA
                     GTCAATTACGGGGCTCTATGAAACAAGAATCGACCTCTCTCAGCTCGGTGGAGACAGC
                     AGGGCTGACCCCAAGAAGAAGAGGAAGGTGAGTGGTGGAGGAAGTGGCGGGTCAGGGT
                     CGATGGACGCGAAATCACTTACGGCATGGTCGAGAACACTGGTTACGTTCAAGGACGT
                     GTTTGTGGACTTTACACGTGAGGAGTGGAAATTGCTGGATACTGCGCAACAAATTGTG
                     TATCGAAATGTCATGCTTGAGAATTACAAGAACCTCGTCAGTCTCGGATACCAGTTGA
                     CGAAACCGGATGTGATCCTTAGGCTCGAAAAGGGGGAAGAACCTTGGCTGGTATCGGG
                     AGGTGGTTCGGGTGGCTCTGGATCAAGCCCAAAGAAGAAACGGAAGGTGGAAGCCTCA
                     GTGCAGGTGAAAAGGGTGCTGGAAAAATCCCCCGGCAAACTCCTCGTGAAGATGCCCT
                     TCCAGGCTTCCCCTGGCGGAAAAGGTGAAGGGGGTGGCGCAACCACATCTGCCCAGGT
                     CATGGTCATCAAGCGACCTGGAAGGAAAAGAAAGGCCGAGGCTGACCCTCAGGCCATT
                     CCAAAGAAACGGGGACGCAAGCCAGGGTCCGTGGTCGCAGCTGCAGCAGCTGAGGCTA
                     AGAAAAAGGCAGTGAAGGAAAGCTCCATCCGCAGTGTGCAGGAGACTGTCCTGCCCAT
                     CAAGAAGAGGAAGACTAGGGAGACCGTGTCCATCGAGGTCAAAGAAGTGGTCAAGCCC
                     CTGCTCGTGTCCACCCTGGGCGAAAAATCTGGAAAGGGGCTCAAAACATGCAAGTCAC
                     CTGGACGGAAAAGCAAGGAGTCTAGTCCAAAGGGGCGCTCAAGCTCCGCTTCTAGTCC
                     CCCTAAAAAGGAACACCATCACCATCACCATCACGCCGAGTCTCCTAAGGCTCCTATG
                     CCACTGCTCCCACCACCTCCACCACCTGAGCCACAGTCAAGCGAAGACCCCATCAGCC
                     CACCCGAGCCTCAGGATCTGTCCTCTAGTATTTGCAAAGAGGAAAAGATGCCCAGAGC
                     AGGCAGCCTGGAGAGTGATGGCTGTCCAAAAGAACCCGCCAAGACCCAGCCTATGGTG
                     GCAGCCGCTGCAACTACCACCACAACCACAACTACCACAGTGGCCGAAAAATACAAGC
                     ATCGCGGCGAGGGCGAACGAAAGGACATTGTGTCAAGCTCCATGCCCAGACCTAACCG
                     GGAGGAACCAGTCGATAGTAGGACACCCGTGACTGAGAGAGTCTCATAG"
                     /note="Geneious type: Editing History Replacement"
     CDS             319..717
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFT
                     GVNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHP
                     GIKAIVKDSDGQPTAVGIRELLPSGYVWEG"
                     /label="BSD"
     primer_bind     320..337
                     /Mismatches=0
                     /%GC="55.6"
                     /Hairpin_Tm="50.7"
                     /Self_Dimer_Tm="0.6"
                     /Tm="57.5"
                     /annotation_group="3299492127870085053877-16f8-47e6-b79a-5
                     90b5f5aad2f"
                     /Sequence="TGGCCAAGCCTTTGTCTC"
                     /created_by="primer3"
                     /label="OPS0038"
     primer_bind     394..413
                     /Mismatches=0
                     /%GC="55.0"
                     /Hairpin_Tm="38.9"
                     /Self_Dimer_Tm="9.8"
                     /Tm="59.8"
                     /annotation_group="32994109742400b37f884b-63b8-4ffa-b792-5
                     1561d1cc6b6"
                     /Sequence="TCTGAAGACTACAGCGTCGC"
                     /created_by="primer3"
                     /label="PCR_0164"
     primer_bind     595..614
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="40.5"
                     /Self_Dimer_Tm="None"
                     /Tm="63.9"
                     /annotation_group="32993992064500f73770dc-658f-45d4-a51f-1
                     aa25ecdfa5d"
                     /Sequence="TGCCGACAGGTGCTTCTCGA"
                     /created_by="primer3"
                     /label="PCR_0042"
     primer_bind     595..614
                     /modified_by="User"
                     /Mismatches=0
                     /%GC="60.0"
                     /Hairpin_Tm="40.5"
                     /Self_Dimer_Tm="None"
                     /Tm="63.9"
                     /annotation_group="329949776298009b3ae493-ffdc-4e06-a0e1-2
                     34b62e2fa29"
                     /Sequence="TGCCGACAGGTGCTTCTCGA"
                     /created_by="primer3"
                     /label="cseq_Blast_out_for"
     primer_bind     600..619
                     /Mismatches=0
                     /%GC="55.0"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.1"
                     /annotation_group="329945766798996f9299f1-0658-49f7-8847-f
                     4f9d4d397dd"
                     /Sequence="ACAGGTGCTTCTCGATCTGC"
                     /created_by="primer3"
                     /label="PCR_0342"
     primer_bind     648..666
                     /Mismatches=0
                     /%GC="63.2"
                     /Hairpin_Tm="36.8"
                     /Self_Dimer_Tm="None"
                     /Tm="60.8"
                     /annotation_group="32994633603800578d72e7-dea2-48d3-bb72-4
                     64c9dc90441"
                     /Sequence="CAGTGATGGACAGCCGACG"
                     /created_by="primer3"
                     /label="PCR_0344"
     primer_bind     736..754
                     /Mismatches=0
                     /%GC="63.2"
                     /Hairpin_Tm="58.1"
                     /Self_Dimer_Tm="7.9"
                     /Tm="62.4"
                     /annotation_group="3299486380790039b44be1-288a-4191-ade5-c
                     5588b4b128d"
                     /Sequence="GCTTGCCTTCTGGCCATGC"
                     /created_by="primer3"
                     /label="prEP0001"
     misc_feature    737^738
                     /Original_Bases="ATCGCTGCCTTCTGCGGGGC"
                     /label="ATCGCTGCCTTCTGCGGGGC"
                     /note="Geneious type: Editing History Deletion"
     cds             <738..824
                     /created_by="User"
                     /modified_by="User"
                     /X-Transferred_From="101362 - pIVTRup Copy; dCas9-VP64"
                     /X-Transferred_Similarity="100.00%"
                     /Transferred_From="101362 - pIVTRup Copy, 101362 - pIVTRup
                     PCR DraI, Digestion of 101362 - pIVTRup PCR DraI -
                     Fragment 3, dCas9-VP64"
                     /Transferred_Similarity="100.00%"
                     /label="5'UTR"
     3'UTR           825..879
                     /created_by="User"
                     /label="polyA(55)"
     misc_feature    complement(881..886)
                     /Recognition_pattern="CGTCTC(1/5)"
                     /annotation_group="CGTCTC"
                     /label="BsmBI"
                     /note="Geneious type: restriction site"
     protein_bind    1025..1041
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /label="lac operator"
     primer_bind     1040..1061
                     /Mismatches=0
                     /%GC="45.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="59.3"
                     /annotation_group="329935097814996a342cae-b747-490a-9717-5
                     05e0310924c"
                     /Sequence="AATTCCACACAACATACGAGCC"
                     /created_by="primer3"
                     /label="PCR_0451"
     primer_bind     1043..1063
                     /Mismatches=0
                     /%GC="57.1"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="62.7"
                     /annotation_group="32994691186199d0561f21-5ce6-452b-be71-2
                     eb01caba03e"
                     /Sequence="TCCACACAACATACGAGCCGG"
                     /created_by="primer3"
                     /label="PCR_0227"
     primer_bind     1044..1061
                     /Mismatches=0
                     /Extension="ACTGGCGAACTACTTACTCTAGCTTCCCGG"
                     /%GC="55.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="55.8"
                     /annotation_group="32993581362800ac1baf9f-8b0e-47bf-8f06-8
                     d5052abcf33"
                     /Sequence="CCACACAACATACGAGCC"
                     /created_by="primer3"
                     /label="PCR_0096"
     primer_bind     1044..1061
                     /Mismatches=0
                     /Extension="TGTGGTGATGTTGAAGAAAACCCTGGTCCA"
                     /%GC="55.6"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="55.8"
                     /annotation_group="32993643118800b12e2fd4-8867-4b30-a097-c
                     71117710ed6"
                     /Sequence="CCACACAACATACGAGCC"
                     /created_by="primer3"
                     /label="PCR_0099"
     promoter        complement(1049..1079)
                     /note="promoter for the E. coli lac operon"
                     /label="lac promoter"
     protein_bind    1094..1115
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence
                      of cAMP."
                     /label="CAP binding site"
     rep_origin      complement(1403..1991)
                     /direction="LEFT"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /label="ori"
     primer_bind     2315..2335
                     /Mismatches=0
                     /%GC="52.4"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="60.2"
                     /annotation_group="329938764343001cebd425-fd9f-4463-8d99-5
                     e473b68f58f"
                     /Sequence="ACCGGCTCCAGATTTATCAGC"
                     /created_by="primer3"
                     /label="qPCR_002"
     primer_bind     2388..2442
                     /Sequence="TCCGCCTCCATCCAGTCTATTAATTGTTGCCGGGAAGCTAGAGTAAG
                     TAGTTCGC"
                     /Mismatches=0
                     /annotation_group="48e043b6-5141-4253-8a86-d2f77acb7e3d"
                     /label="LCR_0007"
     primer_bind     2407..2434
                     /Mismatches=0
                     /%GC="42.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="9.4"
                     /Tm="63.3"
                     /annotation_group="32994171663200f1ecfd36-d54e-46ad-8498-c
                     73b7ca56ea5"
                     /Sequence="TTAATTGTTGCCGGGAAGCTAGAGTAAG"
                     /created_by="primer3"
                     /label="LU-p0024"
     primer_bind     2407..2434
                     /Mismatches=0
                     /%GC="42.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="9.4"
                     /Tm="63.3"
                     /annotation_group="3299423111719977e883ce-0ef0-4434-a252-4
                     9df78bef627"
                     /Sequence="TTAATTGTTGCCGGGAAGCTAGAGTAAG"
                     /created_by="primer3"
                     /label="LU-p0048"
     primer_bind     2407..2434
                     /Mismatches=0
                     /%GC="42.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="9.4"
                     /Tm="63.3"
                     /annotation_group="3299428803000046adcfad-9436-47b2-ad7a-0
                     41279a2dbae"
                     /Sequence="TTAATTGTTGCCGGGAAGCTAGAGTAAG"
                     /created_by="primer3"
                     /label="LU-p0060"
     primer_bind     2407..2440
                     /Mismatches=0
                     /%GC="41.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="9.4"
                     /Tm="66.2"
                     /annotation_group="329943452603996be5728a-8f46-4723-bedf-6
                     9108adf2aaf"
                     /Sequence="TTAATTGTTGCCGGGAAGCTAGAGTAAGTAGTTC"
                     /created_by="primer3"
                     /label="LU-p0073"
     primer_bind     2407..2434
                     /Mismatches=0
                     /%GC="42.9"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="9.4"
                     /Tm="63.3"
                     /annotation_group="32994402928199ee63de49-b6b2-4955-8a72-7
                     1a0180bb41e"
                     /Sequence="TTAATTGTTGCCGGGAAGCTAGAGTAAG"
                     /created_by="primer3"
                     /label="LU-p0076"
     primer_bind     2417..2445
                     /Mismatches=0
                     /%GC="55.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="67.7"
                     /annotation_group="3299381903570032ebfb0a-5869-4ee4-82de-7
                     91c32505a8d"
                     /Sequence="CCGGGAAGCTAGAGTAAGTAGTTCGCCAG"
                     /created_by="primer3"
                     /label="PCR_0067"
     primer_bind     2417..2445
                     /Mismatches=0
                     /%GC="55.2"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="67.7"
                     /annotation_group="32993935700299477fbe74-d4ad-4709-b02a-4
                     0a1facd7ecf"
                     /Sequence="CCGGGAAGCTAGAGTAAGTAGTTCGCCAG"
                     /created_by="primer3"
                     /label="PCR_0015"
     primer_bind     2875..2896
                     /Mismatches=0
                     /%GC="45.5"
                     /Hairpin_Tm="None"
                     /Self_Dimer_Tm="None"
                     /Tm="57.6"
                     /annotation_group="32994807759500e1d49e65-cbc9-46f2-b4b7-d
                     2aa10691f49"
                     /Sequence="TGAGATCCAGTTCGATGTAACC"
                     /created_by="primer3"
                     /label="PCR_0433"
     rep_origin      complement(4178..1)
                     /direction="LEFT"
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /label="f1 ori"
     primer_bind     4535..4553
                     /modified_by="User"
                     /Mismatches=0
                     /%GC="57.9"
                     /Hairpin_Tm="36.2"
                     /Self_Dimer_Tm="None"
                     /Tm="60.3"
                     /annotation_group="32994049305900e324851b-c9ee-4a40-a57b-f
                     25e15ace0ed"
                     /Sequence="TGGCAAGTGTAGCGGTCAC"
                     /created_by="primer3"
                     /label="PCR_0114"
ORIGIN      
        1 tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
       61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
      121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
      181 agggcgaatt gggccctcta gatgcatgct cgagcggccg ccagtgtgat ggatttggac
      241 cctcgtacag aagctaatac gactcactat agggaaataa gagagaaaag aagagtaaga
      301 agaaatataa gagccaccat ggccaagcct ttgtctcaag aagaatccac cctcattgaa
      361 agagcaacgg ctacaatcaa cagcatcccc atctctgaag actacagcgt cgccagcgca
      421 gctctctcta gcgacggccg catcttcact ggtgtcaatg tatatcattt tactggggga
      481 ccttgtgcag aactcgtggt gctgggcact gctgctgctg cggcagctgg caacctgact
      541 tgtatcgtcg cgatcggaaa tgagaacagg ggcatcttga gcccctgcgg acggtgccga
      601 caggtgcttc tcgatctgca tcctgggatc aaagccatag tgaaggacag tgatggacag
      661 ccgacggcag ttgggattcg tgaattgctg ccctctggtt atgtgtggga gggctaaatc
      721 gctgccttct gcggggcttg ccttctggcc atgcccttct tctctccctt gcacctgtac
      781 ctcttggtct ttgaataaag cctgagtagg aagtgagggt ctagaaaaaa aaaaaaaaaa
      841 aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaat gagacgcgat caataaccga
      901 cactcctgcg tcgcctacgc gctacgatct gcagaattcc agcacactgg cggccgttac
      961 tagtggatcc gagctcggta ccaagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     1021 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     1081 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     1141 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     1201 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     1261 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     1321 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     1381 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     1441 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     1501 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     1561 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     1621 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     1681 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     1741 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     1801 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     1861 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     1921 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     1981 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     2041 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     2101 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     2161 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     2221 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     2281 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     2341 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     2401 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     2461 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     2521 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     2581 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     2641 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     2701 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
     2761 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
     2821 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     2881 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     2941 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     3001 cacggaaatg ttgaatactc atactcttcc tttttcaatt cagaagaact cgtcaagaag
     3061 gcgatagaag gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg
     3121 gtcagcccat tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg
     3181 atagcggtcc gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc
     3241 caccatgata ttcggcaagc aggcatcgcc atgggtcacg acgagatcct cgccgtcggg
     3301 catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc
     3361 cagatcatcc tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg
     3421 tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc
     3481 atcagccatg atggatactt tctcggcagg agcaaggtgg gatgacagga gatcctgccc
     3541 cggcacttcg cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc
     3601 tgcgcaagga acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc
     3661 attcagggca ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag
     3721 ccggaacacg gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag
     3781 cctctccacc caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tcatgcgaaa
     3841 cgatcctcat cctgtctctt gatcagatct tgatcccctg cgccatcaga tccttggcgg
     3901 caagaaagcc atccagttta ctttgcaggg cttcccaacc ttaccagagg gcgccccagc
     3961 tggcaattcc ggttcgcttg ctgtccataa aaccgcccag tctagctatc gccatgtaag
     4021 cccactgcaa gctacctgct ttctctttgc gcttgcgttt tcccttgtcc agatagccca
     4081 gtagctgaca ttcatccggg gtcagcaccg tttctgcgga ctggctttct acgtgttccg
     4141 cttcctttag cagcccttgc gccctgaatt ttgttaaaat tcgcgttaaa tttttgttaa
     4201 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     4261 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     4321 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     4381 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     4441 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     4501 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     4561 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcg
//
