LOCUS       Exported                4283 bp ds-DNA     circular SYN 18-APR-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4283)
  AUTHORS   SnapGene License
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 10, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..4283
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     537..553
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             669..687
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="GSTSAS"
     CDS             694..1071
                     /codon_start=1
                     /product="MPEPAKSAPAPKKGSKKAVTKAQKKDGKKRKRSRKESYSIYVYKVLKQV
                     HPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPGELA
                     KHAVSEGTKAVTKYTSAK"
                     /label=HIST1_H2Bj
                     /translation="MPEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKV
                     LKQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLP
                     GELAKHAVSEGTKAITKYTSAK"
     misc_feature    1078..1782
                     /label=mBaoJin
                     /label=mStayGold
                     /note="/vntifkey=21"
     misc_feature    1094..1099
                     /label=SDS
     misc_feature    1795..2383
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     protein_bind    complement(2408..2507)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2548..2564)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2677..3486
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3633..4221
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctgg gatccactag tgctagcgcc accatgccag agccagcgaa gtctgctccc
      721 gccccgaaaa agggctccaa gaaggcggtg actaaggcgc agaagaaagg cggcaagaag
      781 cgcaagcgca gccgcaagga gagctattcc atctatgtgt acaaggttct gaagcaggtc
      841 caccctgaca ccggcatttc gtccaaggcc atgggcatca tgaattcgtt tgtgaacgac
      901 attttcgagc gcatcgcagg tgaggcttcc cgcctggcgc attacaacaa gcgctcgacc
      961 atcacctcca gggagatcca gacggccgtg cgcctgctgc tgcctgggga gttggccaag
     1021 cacgccgtgt ccgagggtac taaggccatc accaagtaca ccagcgctaa gagatctatg
     1081 gtctcaaagg gagaggagga aaacatggct agtacaccat ttaaatttca acttaaagga
     1141 accatcaatg gcaaatcgtt taccgttgaa ggcgaaggtg aagggaactc acatgaaggt
     1201 tctcataaag gaaaatatgt ttgtacaagt ggaaaactac cgatgtcatg ggcagcactt
     1261 gggacaacct ttggttatgg aatgaaatat tataccaaat atcctagtgg actgaagaac
     1321 tggtttcgtg aagtaatgcc cggaggcttt acctacgatc gtcatattca atataaaggc
     1381 gatgggagta tccatgcaaa acaccaacac tttatgaaaa atgggactta tcacaacatt
     1441 gtagaattta ccggtcagga ttttaaagaa aatagtccag tcttaactgg agatatgaat
     1501 gtctcattac cgaatgaagt cccacaaata cccagagatg atggagtaga atgcccagtg
     1561 accttgcttt atcctttatt atcggataaa tcaaaatacg tcgaggctca ccaatataca
     1621 atctgcaagc ctcttcataa tcaaccagca cctgatgtcc catatcactg gattcgtaaa
     1681 caatacacac aaagcaaaga tgatgccgag gaacgcgatc atatctgtca atcagagact
     1741 ctcgaagcac acttaaaggg catggacgag ctgtataagt agaagcttat cgataatcaa
     1801 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     1861 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     1921 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     1981 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     2041 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     2101 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     2161 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     2221 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     2281 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     2341 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata cgcgtgttaa
     2401 ctctagaacc cagctttctt gtacaaagtt ggcattataa gaaagcattg cttatcaatt
     2461 tgttgcaacg aacaggtcac tatcagtcaa aataaaatca ttatttgcca tccagctgat
     2521 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2581 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg aacaataaaa
     2641 ctgtctgctt acataaacag taatacaagg ggtgttatga gccatattca acgggaaacg
     2701 tcgaggccgc gattaaattc caacatggat gctgatttat atgggtataa atgggctcgc
     2761 gataatgtcg ggcaatcagg tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca
     2821 gagttgtttc tgaaacatgg caaaggtagc gttgccaatg atgttacaga tgagatggtc
     2881 agactaaact ggctgacgga atttatgcct cttccgacca tcaagcattt tatccgtact
     2941 cctgatgatg catggttact caccactgcg atccccggaa aaacagcatt ccaggtatta
     3001 gaagaatatc ctgattcagg tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg
     3061 ttgcattcga ttcctgtttg taattgtcct tttaacagcg atcgcgtatt tcgtctcgct
     3121 caggcgcaat cacgaatgaa taacggtttg gttgatgcga gtgattttga tgacgagcgt
     3181 aatggctggc ctgttgaaca agtctggaaa gaaatgcata aacttttgcc attctcaccg
     3241 gattcagtcg tcactcatgg tgatttctca cttgataacc ttatttttga cgaggggaaa
     3301 ttaataggtt gtattgatgt tggacgagtc ggaatcgcag accgatacca ggatcttgcc
     3361 atcctatgga actgcctcgg tgagttttct ccttcattac agaaacggct ttttcaaaaa
     3421 tatggtattg ataatcctga tatgaataaa ttgcagtttc atttgatgct cgatgagttt
     3481 ttctaatcag aattggttaa ttggttgtaa cactggcaga gcattacgct gacttgacgg
     3541 gacggcgcaa gctcatgacc aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg
     3601 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     3661 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     3721 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     3781 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     3841 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     3901 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     3961 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     4021 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     4081 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     4141 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     4201 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     4261 tgctggcctt ttgctcacat gtt
//
