LOCUS       Exported                3663 bp ds-DNA     circular SYN 11-JUN-2015
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3663)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 10, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3663
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     terminator      268..295
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      387..473
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     536..553
                     /label=M13-fwd
     protein_bind    569..668
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     misc_feature    679..684
                     /label=TOPO Site 5'
     misc_feature    689..697
                     /label=Kozak
     CDS             698..1741
                     /codon_start=1
                     /label=DRE recombinase
                     /translation="MGASELIISGSSGGFLRNIGKEYQEAAENFMRFMNDQGAYAPNTL
                     RDLRLVFHSWARWCHARQLAWFPISPEMAREYFLQLHDADLASTTIDKHYAMLNMLLSH
                     CGLPPLSDDKSVSLAMRRIRREAATEKGERTGQAIPLRWDDLKLLDVLLSRSERLVDLR
                     NRAFLFVAYNTLMRMSEISRIRVGDLDQTGDTVTLHISHTKTITTAAGLDKVLSRRTTA
                     VLNDWLDVSGLREHPDAVLFPPIHRSNKARITTTPLTAPAMEKIFSDAWVLLNKRDATP
                     NKGRYRTWTGHSARVGAAIDMAEKQVSMVEIMQEGTWKKPETLMRYLRRGGVSVGANSR
                     LMDSASGA"
     misc_feature    1742..1768
                     /label=NLS-stop
     CDS             1745..1765
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    1772..1777
                     /label=TOPO SITE 3'
     protein_bind    complement(1788..1887)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(1905..1923)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1928..1944)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             2057..2866
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     rep_origin      3013..3601
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
       61 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
      121 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
      181 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaata cgcgtaccgc
      241 tagccaggaa gagtttgtag aaacgcaaaa aggccatccg tcaggatggc cttctgctta
      301 gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc accctccggg ccgttgcttc
      361 acaacgttca aatccgctcc cggcggattt gtcctactca ggagagcgtt caccgacaaa
      421 caacagataa aacgaaaggc ccagtcttcc gactgagcct ttcgttttat ttgatgcctg
      481 gcagttccct actctcgcgt taacgctagc atggatgttt tcccagtcac gacgttgtaa
      541 aacgacggcc agtcttaagc tcgggcccca aataatgatt ttattttgac tgatagtgac
      601 ctgttcgttg caacaaattg atgagcaatg cttttttata atgccaactt tgtacaaaaa
      661 agcaggctcc gcggccgccc ccttcaccgt cgccaccatg ggtgctagcg agctgatcat
      721 ctctggctcc tctggaggat tcctgaggaa catcggcaag gagtaccagg aggctgctga
      781 gaacttcatg agattcatga atgaccaggg agcctacgcc cctaacaccc tgagagacct
      841 gaggctggtg ttccactcct gggctagatg gtgccacgct agacagctgg cctggttccc
      901 tatctctcct gagatggcta gggagtactt ccttcagctg cacgatgctg acctggcctc
      961 taccaccatc gacaagcact acgccatgct gaacatgctg ctgtcccact gtggcctgcc
     1021 tcctctgtct gatgacaagt ctgtgagcct ggccatgagg agaatccgga gagaggctgc
     1081 caccgagaag ggagagagaa ccggccaggc catccctctg agatgggatg acctgaagct
     1141 gctggatgtg ctgctgtcta gatctgagag actggtggac ctgaggaata gggccttcct
     1201 gtttgtggcc tacaacaccc tgatgaggat gtctgagatc tctaggatca gagtgggaga
     1261 cctggaccag accggagaca ccgtgaccct gcacatctcc cacaccaaga ccatcaccac
     1321 cgctgctggc ctggacaaag tgctgtctag gaggaccacc gctgtgctga atgactggct
     1381 ggatgtgtct ggcctgagag agcaccctga cgctgtgctg ttccctccta tccaccggag
     1441 caacaaggct aggatcacca ccacccctct gaccgcccct gccatggaga agatttttag
     1501 cgatgcctgg gtgctgctga acaagaggga tgccacccct aacaagggcc gctaccggac
     1561 ctggaccggc cactctgcta gagtgggagc tgccatcgac atggctgaga agcaagtgtc
     1621 catggtggag atcatgcagg agggcacctg gaaaaagcct gagacactga tgagatacct
     1681 gaggagggga ggagtgtctg tgggagccaa ctctaggctg atggactccg ctagcggcgc
     1741 cggtcctaag aagaagagga aagtgtgatg aaagggtggg cgcgccgacc cagctttctt
     1801 gtacaaagtt ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac
     1861 tatcagtcaa aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta
     1921 ttacatggtc atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta
     1981 cattgcacaa gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag
     2041 taatacaagg ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc
     2101 caacatggat gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg
     2161 tgcgacaatc tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg
     2221 caaaggtagc gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga
     2281 atttatgcct cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact
     2341 caccactgcg atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg
     2401 tgaaaatatt gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg
     2461 taattgtcct tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa
     2521 taacggtttg gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca
     2581 agtctggaaa gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg
     2641 tgatttctca cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt
     2701 tggacgagtc ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg
     2761 tgagttttct ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga
     2821 tatgaataaa ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa
     2881 ttggttgtaa cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc
     2941 aaaatccctt aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat
     3001 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     3061 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     3121 ggtaactggc ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt
     3181 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     3241 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     3301 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     3361 ggagcgaacg acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac
     3421 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     3481 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     3541 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     3601 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     3661 gtt
//
