LOCUS       Exported                9285 bp ds-DNA     circular SYN 04-AUG-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        complement(260..364)
                     /label=AmpR promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     enhancer        395..412
                     /label=CMV enhancer (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=4.8"
                     /note="/other=enhancer"
     promoter        413..4382
                     /label=Mef2C AHF Promoter
     protein_bind    complement(2424..2431)
                     /label=Tbx5 Binding Site (ECR Browser)
     primer_bind     4104..4123
                     /label=Mef2c-AHF-Pro-Fwd
     misc_feature    4310..4332
                     /label=JDW DRE FOR 1
     intron          4398..4438
                     /label=beta-globin intron (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=97.6"
                     /note="/match_length=7.2"
                     /note="/other=intron"
     intron          4439..4491
                     /label=rabbit B-globin intron
     protein_bind    4505..4529
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      4542..4551
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     regulatory      4542..4551
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             4548..5591
                     /codon_start=1
                     /label=DRE recombinase
                     /translation="MGASELIISGSSGGFLRNIGKEYQEAAENFMRFMNDQGAYAPNTL
                     RDLRLVFHSWARWCHARQLAWFPISPEMAREYFLQLHDADLASTTIDKHYAMLNMLLSH
                     CGLPPLSDDKSVSLAMRRIRREAATEKGERTGQAIPLRWDDLKLLDVLLSRSERLVDLR
                     NRAFLFVAYNTLMRMSEISRIRVGDLDQTGDTVTLHISHTKTITTAAGLDKVLSRRTTA
                     VLNDWLDVSGLREHPDAVLFPPIHRSNKARITTTPLTAPAMEKIFSDAWVLLNKRDATP
                     NKGRYRTWTGHSARVGAAIDMAEKQVSMVEIMQEGTWKKPETLMRYLRRGGVSVGANSR
                     LMDSASGA"
     CDS             5619..6554
                     /codon_start=1
                     /product="mutated ligand-binding domain of the human 
                     estrogen receptor (Feil et al., 1997)"
                     /label=ERT2
                     /translation="AGDMRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPIL
                     YSEYDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEI
                     LMIGLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEF
                     VCLKSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRL
                     AQLLLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEET
                     DQSHLATAGSTSSHSLQKYYITGEAEGFPAT"
     CDS             5628..6557
                     /codon_start=1
                     /label=ERT2mut (G400V/L543A/L544A/M258A/L259A)
                     /translation="MRAANLWPSPLMIKRSKKNSLALSLTADQMVSALLDAEPPILYSE
                     YDPTRPFSEASMMGLLTNLADRELVHMINWAKRVPGFVDLTLHDQVHLLECAWLEILMI
                     GLVWRSMEHPVKLLFAPNLLLDRNQGKCVEGMVEIFDMLLATSSRFRMMNLQGEEFVCL
                     KSIILLNSGVYTFLSSTLKSLEEKDHIHRVLDKITDTLIHLMAKAGLTLQQQHQRLAQL
                     LLILSHIRHMSNKGMEHLYSMKCKNVVPLYDLLLEAADAHRLHAPTSRGGASVEETDQS
                     HLATAGSTSSHSLQKYYITGEAEGFPATA"
     protein_bind    complement(6581..6605)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6620..6691
                     /label=HBB (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=95.8"
                     /note="/match_length=66.7"
                     /note="/other=CDS"
     polyA_signal    6762..6817
                     /label=beta-globin poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     primer_bind     complement(7178..7194)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    complement(7202..7218)
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        complement(7226..7256)
                     /label=lac promoter
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=promoter"
     protein_bind    complement(7271..7292)
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     CDS             complement(7305..7344)
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
     promoter        7351..7546
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        7351..7373
                     /label=SV40 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=7.7"
                     /note="/other=promoter"
     rep_origin      7397..7532
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    7552..7686
                     /label=SV40 poly(A) signal
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=polyA_signal"
     misc_feature    complement(7589..7608)
                     /label=SV40pA REVSeq
     CDS             complement(7688..7742)
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     rep_origin      complement(7925..8513)
                     /direction=LEFT
                     /label=ori
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=rep_origin"
     CDS             complement(join(8684..9285,1..259))
                     /label=AmpR
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.8"
                     /note="/match_length=100.0"
                     /note="/other=CDS"
ORIGIN
        1 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
       61 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
      121 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
      181 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
      241 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
      301 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
      361 cgcgcacatt tccccgaaaa gtgccacctg ggtcgacatt gattattgac tacgagagga
      421 aagccaccag tttctttgtc ttgaagtatt tatttaaaat gttggcatgt gtgacagtga
      481 tacagttaaa tggccatgct cttctttatg atatgtgcag tgctgtggca tggtggttgc
      541 tcagagctcc taaaaccttc caagggccac ctttgcaagt gcctactaca attaagctga
      601 ttcctaggtg gccatgtaca tcatcatagg acaaactaca aagaggttaa aatatctttt
      661 acattgtgga atttgaatct taccatgttt ctcggatttt tatttttcta aattttcatg
      721 ttcaattata tttatgatga aagtattctg gctgaagaac aaatactata cacttgaata
      781 atcttgaggg gtagattaat ctatatcctt tcttcatctc agggtaggtt gctgtggcaa
      841 ccgccccagc tcactttaaa ataatttttt aaagctcact tcaagagcta cacccagcgt
      901 gggccaggct taaaagcagg cagacccagt gcatagggct ttcgtagact gctgctttaa
      961 tgtagggagg cagaagaaag ggagctgaag taagatggct caaaacagaa attggaatgt
     1021 tagctttgag ctgaaacaat caatcaccat gctctctgta acttcaggtg tctaaatgca
     1081 ttgtgaaata aatccttgga tgagataact aatgtttatt tatatttaaa attaagcact
     1141 tataataatt aatgaaagga atatttgtaa ttacacacat tcagagagag aaggggagag
     1201 ggggagaagg agagagagga agtgagtgag tgattgagag agagagagag agagagaggg
     1261 agggagggag agggaggtaa ggagaaaggt ggtttaatgg caggtcaatt ctagccatca
     1321 cttcatcttc tttattaaag gaaagtttcc ttttattaaa tccttagctt cactccccac
     1381 tgatataaaa gatataataa ttatgattat tttcaagcat agaacaaaat tggagagctc
     1441 atgcaaacca ggaaaccgag gcctagaaag gatttatcta ttaggaagac acttttagtg
     1501 ttttcaatgt aaactatcaa accataagta aaaaagttag tagtgctctg ccacagagtc
     1561 acatctacaa ccacaaatcc caaactggat agagcattct aagtctctca acatctagaa
     1621 caaaatactt cctacacaag aaaaatgaat accattaggt agcaaggatc accagtttag
     1681 gtaaataaaa gtaaagagat aatgtgtgtc tggcattact taaagacaaa tataaaacac
     1741 actctggatt aacaatctca tggattacta ctaaattatt tacttattta tttacttata
     1801 tatatattta tttattttcc acttcagcta caaggtttgt gaggaaaact aacactaatc
     1861 ttaactttgc tcacacatca ttaagaagct aaatatacaa agggggaaaa tcttatcttt
     1921 tcaaaggcca gctgtaatac attccagacc caaagtttca aatcatcctc acctggactg
     1981 gacatacttt taaatgttta caatattgtt gataaatacg ggtttgttac aatacttatt
     2041 actaaacaat tctttgacac ctggtgggga aaaataaaag tttccttttg aaggaaaata
     2101 aatcaaaact gaagtgtgct gtggtaagta gaaactttgt ctataattaa ggagaagagc
     2161 atgattgaat tcatgtattg gctatatcaa ctctgtcatg taacataggc cattatagtt
     2221 cattatacta atttaaagca ctatgagaat aagttctctt ggtccttctt atagtttgct
     2281 ctgtgaggat ctttgtaaac cttaaaaata atctaaattt ttatttatat caccatatct
     2341 aggtatgcct tagcatgcag catgtctaag ctcagagctt gtacctctaa acatgccgtt
     2401 ctgtttatat aaactcatgg tagtcacact tgctatgtga aagaggagca aatacaatat
     2461 agcatagata ccattttcaa aagcagaaca aacaaaaaac ccaccatgtc acaaagcttg
     2521 tgctctgtga cagcttgtca tgctttcctg taataaaatc gcagtactaa ttaatagtct
     2581 cagtggtaag gagccagagg tttgaagatt gttcccatgt agtttaactt gcctgtaaaa
     2641 tcaactttct gctttcaaat gaagccttcc ttcggagggt gcttaaatgt tgagtccact
     2701 gctctgatag aagctactaa cttcaaacag cacaatgagg caggaggctt ttttgatgca
     2761 caatgcccct ctgcattaaa atagtactct gcaggcctac tggagagctt cccgggaaat
     2821 tcagccagtt actgggttaa acatatactt tcgaaaatat ttttaattag ctagatattt
     2881 ctatgggcta ctattgtcta ctcttgtatc ttctacatta tagtactctg tgaactttct
     2941 cttcctctaa tcatagtatt aaaaattaaa tgttcatatt ttacacaagc tacagtactc
     3001 ctggcatgca atctttcact attttgagaa tgcatagggt tgacttgtat atagttgata
     3061 gatttaatct ttagcttttt aggtgtggtc gggatatcta gtaccttttt tttttctaag
     3121 tatcaattct ttgttaacct atatttttac tatggaaaat tattcgacag cttaaacctt
     3181 aacctaagag ttcttatcag tgtctgctcc tgcttcctgt gcagctgaaa ctcttcagga
     3241 taagtgaagt cacccgctat ctatcggtca ggggagccta atgcatttgg gaagttgttt
     3301 ttggtttact tgctaatgac ctggataaag tccagtaagg tgtggaggga aggagggggc
     3361 attctccgag gaagctgtcc tctcagtaaa tttgtccaga ttgattcgaa agttaatggc
     3421 ccggggataa agtcgagatt tcaaaaactt gtaattaatt tattactaac attggaggat
     3481 cctttcggct cactttctag cctagccctc ctgactctca aggccaaaag ctttatacat
     3541 tctgggcggg caggaagcgc acgctgggat cgaggcttgc agacatcctg gatcgtggct
     3601 tttacgcgtg agtaacggag gcgcgcgcgg accggagcaa ggatgaagtg gctactgaga
     3661 atctaaaggg ggcggggggg ggggggggag tcactagagc agctcttagg gtcgcgctcc
     3721 tattggtcag aaggaagatc tcgacttcag aaggactttc ctgctagctg gcttgcagcc
     3781 tccccggggg agcgagcgcg caggagacta gcgtcctctg tagcggcggg gagagaggca
     3841 gggagccgcc gctccccgtg ttgtcttccc tttcatgcaa taaagaattg tcattaggtt
     3901 tgcgtatggc atgtgccatt accccaccgt aaaactaaaa ttagcaaaat gtcaggaatg
     3961 gaaagattga ttcaccaaga tgcaattatc atttaaaagt gcttgattga ggtactgatg
     4021 ttcagtgatt tattctgcat accatataca taattaaagt agtgtagtgg agtaatttat
     4081 caatctagtt gagactggag gggtgaggag ggagctgcag tatgtttgtt ttattaaaat
     4141 gttgcgaggt tctagtcccg cccccctttt gcaagagtga aactgatgat ttccccagct
     4201 cgggaggaga ggcaacggtt tgggctggtt gggatagaga aacggaaagg aaaaggcagc
     4261 gccaggcaaa ggcaagaata aaatgaattg ggggacgggg gtagcaccgg agcgtacgtg
     4321 ctgcttagaa ccccagcccc gtgtgctccg tcttgcttgc cgcccccgcg tcccctccca
     4381 gtcgaggtcg actagagcct ctgctaacca tgttcatgcc ttcttctttt tcctacagct
     4441 cctgggcaac gtgctggtta ttgtgctgtc tcatcatttt ggcaaagaat tcctcgagga
     4501 tatcacaagt ttgtacaaaa aagcaggctg aattcgtcga cgccaccatg ggtgctagcg
     4561 agctgatcat ctctggctcc tctggaggat tcctgaggaa catcggcaag gagtaccagg
     4621 aggctgctga gaacttcatg agattcatga atgaccaggg agcctacgcc cctaacaccc
     4681 tgagagacct gaggctggtg ttccactcct gggctagatg gtgccacgct agacagctgg
     4741 cctggttccc tatctctcct gagatggcta gggagtactt ccttcagctg cacgatgctg
     4801 acctggcctc taccaccatc gacaagcact acgccatgct gaacatgctg ctgtcccact
     4861 gtggcctgcc tcctctgtct gatgacaagt ctgtgagcct ggccatgagg agaatccgga
     4921 gagaggctgc caccgagaag ggagagagaa ccggccaggc catccctctg agatgggatg
     4981 acctgaagct gctggatgtg ctgctgtcta gatctgagag actggtggac ctgaggaata
     5041 gggccttcct gtttgtggcc tacaacaccc tgatgaggat gtctgagatc tctaggatca
     5101 gagtgggaga cctggaccag accggagaca ccgtgaccct gcacatctcc cacaccaaga
     5161 ccatcaccac cgctgctggc ctggacaaag tgctgtctag gaggaccacc gctgtgctga
     5221 atgactggct ggatgtgtct ggcctgagag agcaccctga cgctgtgctg ttccctccta
     5281 tccaccggag caacaaggct aggatcacca ccacccctct gaccgcccct gccatggaga
     5341 agatttttag cgatgcctgg gtgctgctga acaagaggga tgccacccct aacaagggcc
     5401 gctaccggac ctggaccggc cactctgcta gagtgggagc tgccatcgac atggctgaga
     5461 agcaagtgtc catggtggag atcatgcagg agggcacctg gaaaaagcct gagacactga
     5521 tgagatacct gaggagggga ggagtgtctg tgggagccaa ctctaggctg atggactccg
     5581 ctagcggcgc cagacgcata tgcgtacgcg gatcctctgc tggagacatg agagctgcca
     5641 acctttggcc aagcccgctc atgatcaaac gctctaagaa gaacagcctg gccttgtccc
     5701 tgacggccga ccagatggtc agtgccttgt tggatgctga gccccccata ctctattccg
     5761 agtatgatcc taccagaccc ttcagtgaag cttcgatgat gggcttactg accaacctgg
     5821 cagacaggga gctggttcac atgatcaact gggcgaagag ggtgccaggc tttgtggatt
     5881 tgaccctcca tgatcaggtc caccttctag aatgtgcctg gctagagatc ctgatgattg
     5941 gtctcgtctg gcgctccatg gagcacccag tgaagctact gtttgctcct aacttgctct
     6001 tggacaggaa ccagggaaaa tgtgtagagg gcatggtgga gatcttcgac atgctgctgg
     6061 ctacatcatc tcggttccgc atgatgaatc tgcagggaga ggagtttgtg tgcctcaaat
     6121 ctattatttt gcttaattct ggagtgtaca catttctgtc cagcaccctg aagtctctgg
     6181 aagagaagga ccatatccac cgagtcctgg acaagatcac agacactttg atccacctga
     6241 tggccaaggc aggcctgacc ctgcagcagc agcaccagcg gctggcccag ctcctcctca
     6301 tcctctccca catcaggcac atgagtaaca aaggcatgga gcatctgtac agcatgaagt
     6361 gcaagaacgt ggtgcccctc tatgacctgc tgctggaggc ggcggacgcc caccgcctac
     6421 atgcgcccac tagccgtgga ggggcatccg tggaggagac ggaccaaagc cacttggcca
     6481 ctgcgggctc tacttcatcg cattccttgc aaaagtatta catcacgggg gaggcagagg
     6541 gtttccctgc cacagcttga gcggccgcct cgaggaattc acccagcttt cttgtacaaa
     6601 gtggtgatat cgcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg
     6661 tgtggccaat gccccggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta
     6721 tggggacatc atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt
     6781 cattgcaata gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc
     6841 aaatcattta aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat
     6901 gctggctgcc atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc
     6961 tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt
     7021 ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc
     7081 cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag
     7141 atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     7201 attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     7261 ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     7321 agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa ccatagtccc
     7381 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     7441 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     7501 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc taacttgttt
     7561 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     7621 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     7681 tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     7741 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     7801 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     7861 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     7921 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     7981 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     8041 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     8101 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     8161 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     8221 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     8281 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     8341 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     8401 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     8461 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8521 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8581 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8641 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8701 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8761 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     8821 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     8881 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     8941 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9001 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9061 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9121 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9181 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9241 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgct
//
