LOCUS       Exported                9506 bp ds-DNA     circular SYN 04-APR-2022
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Wythe Lab
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9506
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      4..459
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     enhancer        729..1032
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1035..1310
                     /label=chicken beta-actin promoter
     intron          1311..2319
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     exon            1311..1400
                     /label=chicken B-actin exon
     intron          1401..2319
                     /label=chicken b-actin intron + rabbit b-globin intron
     intron          2320..2372
                     /label=rabbit B-globin intron
     misc_recomb     2380..2411
                     /label=Rox
                     /note="Rox recombination site, Sauer and McDermott 2004"
     regulatory      2426..2435
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             2459..2479
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2483..2503
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2507..2527
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             2543..3238
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             3239..3280
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     polyA_signal    3452..3507
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_recomb     3849..3880
                     /label=Rox
                     /note="Rox recombination site, Sauer and McDermott 2004"
     primer_bind     3896..3924
                     /label=oJDW 2068
     protein_bind    3900..3924
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3932..3941
                     /regulatory_class="other"
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             3938..3961
                     /codon_start=1
                     /label=Myr Tag
                     /note="MARCKS myristoylation peptide (MGCCFSKT)"
                     /translation="MGCCFSKT"
     CDS             3962..4660
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     misc_feature    4661..4726
                     /label=MCS
                     /note="multiple cloning site"
     CDS             4739..4762
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(4774..4803)
                     /label=oJDW 2069
     protein_bind    complement(4774..4798)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    4955..5010
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     misc_recomb     complement(5386..5419)
                     /label=FRT
     polyA_signal    5439..5900
                     /label=PGK poly(A) signal
                     /note="mouse phosphoglycerate kinase 1 polyadenylation 
                     signal"
     CDS             complement(5910..6713)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(6727..7226)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     misc_recomb     complement(7242..7275)
                     /label=FRT
     promoter        complement(7318..7336)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7357..7373)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    7381..7397
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(7405..7435)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7450..7471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7759..8347)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(8354..8363)
                     /label=TCF/LEF DBD
     CDS             complement(8518..9378)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(9379..9483)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctgacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
       61 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
      121 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
      181 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg
      241 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
      301 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt
      361 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
      421 ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccgg
      661 cgcgccacta gttattaata gtaatcaatt acggggtcat tagttcatag cccatatatg
      721 gagttccgcg ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc
      781 cgcccattga cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat
      841 tgacgtcaat gggtggacta tttacggtaa actgcccact tggcagtaca tcaagtgtat
      901 catatgccaa gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat
      961 gcccagtaca tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc
     1021 gctattacca tgggtcgagg tgagccccac gttctgcttc actctcccca tctccccccc
     1081 ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag cgatgggggc
     1141 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
     1201 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
     1261 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg
     1321 cgttgccttc gccccgtgcc ccgctccgcg ccgcctcgcg ccgcccgccc cggctctgac
     1381 tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg ggctgtaatt
     1441 agcgcttggt ttaatgacgg ctcgtttctt ttctgtggct gcgtgaaagc cttaaagggc
     1501 tccgggaggg ccctttgtgc gggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc
     1561 gtggggagcg ccgcgtgcgg cccgcgctgc ccggcggctg tgagcgctgc gggcgcggcg
     1621 cggggctttg tgcgctccgc gtgtgcgcga ggggagcgcg gccgggggcg gtgccccgcg
     1681 gtgcgggggg gctgcgaggg gaacaaaggc tgcgtgcggg gtgtgtgcgt gggggggtga
     1741 gcagggggtg tgggcgcggc ggtcgggctg taaccccccc ctgcaccccc ctccccgagt
     1801 tgctgagcac ggcccggctt cgggtgcggg gctccgtgcg gggcgtggcg cggggctcgc
     1861 cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc ggggcggggc cgcctcgggc
     1921 cggggagggc tcgggggagg ggcgcggcgg ccccggagcg ccggcggctg tcgaggcgcg
     1981 gcgagccgca gccattgcct tttatggtaa tcgtgcgaga gggcgcaggg acttcctttg
     2041 tcccaaatct ggcggagccg aaatctggga ggcgccgccg caccccctct agcgggcgcg
     2101 ggcgaagcgg tgcggcgccg gcaggaagga aatgggcggg gagggccttc gtgcgtcgcc
     2161 gcgccgccgt ccccttctcc atctccagcc tcggggctgc cgcaggggga cggctgcctt
     2221 cgggggggac ggggcagggc ggggttcggc ttctggcgtg tgaccggcgg ctctagagcc
     2281 tctgctaacc atgttcatgc cttcttcttt ttcctacagc tcctgggcaa cgtgctggtt
     2341 attgtgctgt ctcatcattt tggcaaagaa ttcaagcttt aactttaaat aatgccaatt
     2401 atttaaagtt actaggttcc cgcgggccac catggccaca accatggtgc acgtggatcc
     2461 aaaaaagaag agaaaggtag atccaaaaaa gaagagaaag gtagatccaa aaaagaagag
     2521 aaaggtacac gtgtccacgc gtatggtgag cgagctgatt aaggagaaca tgcacatgaa
     2581 gctgtacatg gagggcaccg tgaacaacca ccacttcaag tgcacatccg agggcgaagg
     2641 caagccctac gagggcaccc agaccatgag aatcaaggcg gtcgagggcg gccctctccc
     2701 cttcgccttc gacatcctgg ctaccagctt catgtacggc agcaaaacct tcatcaacca
     2761 cacccagggc atccccgact tctttaagca gtccttcccc gagggcttca catgggagag
     2821 agtcaccaca tacgaagacg ggggcgtgct gaccgctacc caggacacca gcctccagga
     2881 cggctgcctc atctacaacg tcaagatcag aggggtgaac ttcccatcca acggccctgt
     2941 gatgcagaag aaaacactcg gctgggaggc ctccaccgag accctgtacc ccgctgacgg
     3001 cggcctggaa ggcagagccg acatggccct gaagctcgtg ggcgggggcc acctgatctg
     3061 caacttgaag accacataca gatccaagaa acccgctaag aacctcaaga tgcccggcgt
     3121 ctactatgtg gacagaagac tggaaagaat caaggaggcc gacaaagaga cctacgtcga
     3181 gcagcacgag gtggctgtgg ccagatactg cgacctccct agcaaactgg ggcacagagg
     3241 taagcctatc cctaaccctc tcctcggtct cgattctacg tagccgcggg agctaaccta
     3301 ggcggccgca ctcctcaggt gcaggctgcc tatcagaagg tggtggctgg tgtggccaat
     3361 gccctggctc acaaatacca ctgagatctt tttccctctg ccaaaaatta tggggacatc
     3421 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata
     3481 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta
     3541 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgccatatg ctggctgcca
     3601 tgaacaaagg tggctataaa gaggtcatca gtatatgaaa cagccccctg ctgtccattc
     3661 cttattccat agaaaagcct tgacttgagg ttagattttt tttatatttt gttttgtgtt
     3721 atttttttct ttaacatccc taaaattttc cttacatgtt ttactagcca gatttttcct
     3781 cctctcctga ctactcccag tcatagctgt ccctcttctc ttatgaagat ccctcgacct
     3841 gcagctagta actttaaata atgccaatta tttaaagtta ctaggatctc gaggatatca
     3901 caagtttgta caaaaaagca ggctttaaac ggccaccatg ggttgctgtt tctccaagac
     3961 catgagcgag ctgattaagg agaacatgca catgaagctg tacatggagg gcaccgtgga
     4021 caaccatcac ttcaagtgca catccgaggg cgaaggcaag ccctacgagg gcacccagac
     4081 catgagaatc aaggtggtcg agggcggccc tctccccttc gccttcgaca tcctggctac
     4141 tagcttcctc tacggcagca agaccttcat caaccacacc cagggcatcc ccgacttctt
     4201 caagcagtcc ttccctgagg gcttcacatg ggagagagtc accacatacg aagacggggg
     4261 cgtgctgacc gctacccagg acaccagcct ccaggacggc tgcctcatct acaacgtcaa
     4321 gatcagaggg gtgaacttca catccaacgg ccctgtgatg cagaagaaaa cactcggctg
     4381 ggaggccttc accgagacgc tgtaccccgc tgacggcggc ctggaaggca gaaacgacat
     4441 ggccctgaag ctcgtgggcg ggagccatct gatcgcaaac atcaagacca catatagatc
     4501 caagaaaccc gctaagaacc tcaagatgcc tggcgtctac tatgtggact acagactgga
     4561 aagaatcaag gaggccaaca acgagaccta cgtcgagcag cacgaggtgg cagtggccag
     4621 atactgcgac ctccctagca aactgggaca caagcttaat tccggactca gatctcgagc
     4681 tcaagcttcg aattctgcag tcgacggtac cgcgggcccg ggatccaccg gatctagaga
     4741 ctacaaagac gatgacgaca agtagccatc tagacccagc tttcttgtac aaagtggtga
     4801 tatcgcggcc gcactcctca ggtgcaggct gcctatcaga aggtggtggc tggtgtggcc
     4861 aatgccctgg ctcacaaata ccactgagat ctttttccct ctgccaaaaa ttatggggac
     4921 atcatgaagc cccttgagca tctgacttct ggctaataaa ggaaatttat tttcattgca
     4981 atagtgtgtt ggaatttttt gtgtctctca ctcggaagga catatgggag ggcaaatcat
     5041 ttaaaacatc agaatgagta tttggtttag agtttggcaa catatgccat atgctggctg
     5101 ccatgaacaa aggtggctat aaagaggtca tcagtatatg aaacagcccc ctgctgtcca
     5161 ttccttattc catagaaaag ccttgacttg aggttagatt ttttttatat tttgttttgt
     5221 gttatttttt tctttaacat ccctaaaatt ttccttacat gttttactag ccagattttt
     5281 cctcctctcc tgactactcc cagtcatagc tgtccctctt ctcttatgaa gatccctcga
     5341 cctgcagccc aagcttatcc tagattttcc accgcggtgg cggccgaagt tcctatactt
     5401 tctagagaat aggaacttcc tcgacggtat cgataagctt ctgatggaat tagaacttgg
     5461 caaaacaata ctgagaatga agtgtatgtg gaacagaggc tgctgatctc gttcttcagg
     5521 ctatgaaact gacacatttg gaaaccacag tacttagaac cacaaagtag gaatcaagag
     5581 aaaaacaatg atcccacgag agatctatag atctatagat catgagtggg aggaatgagc
     5641 tggcccttaa tttggttttg cttgtttaaa ttatgatatc caactatgaa acattatcat
     5701 aaagcaatag taaagagcct tcagtaaaga gcaggcattt atctaatccc accccacccc
     5761 cacccccgta gctccaatcc ttccattcaa aatgtaggta ctctgttctc acccttctta
     5821 acaaagtatg acaggaaaaa cttccatttt agtggacatc tttattgttt aatagatcat
     5881 caatttctgc agacttacag cggatcccct cagaagaact cgtcaagaag gcgatagaag
     5941 gcgatgcgct gcgaatcggg agcggcgata ccgtaaagca cgaggaagcg gtcagcccat
     6001 tcgccgccaa gctcttcagc aatatcacgg gtagccaacg ctatgtcctg atagcggtcc
     6061 gccacaccca gccggccaca gtcgatgaat ccagaaaagc ggccattttc caccatgata
     6121 ttcggcaagc aggcatcgcc atgggtcacg acgagatcat cgccgtcggg catgcgcgcc
     6181 ttgagcctgg cgaacagttc ggctggcgcg agcccctgat gctcttcgtc cagatcatcc
     6241 tgatcgacaa gaccggcttc catccgagta cgtgctcgct cgatgcgatg tttcgcttgg
     6301 tggtcgaatg ggcaggtagc cggatcaagc gtatgcagcc gccgcattgc atcagccatg
     6361 atggatactt tctcggcagg agcaaggtga gatgacagga gatcctgccc cggcacttcg
     6421 cccaatagca gccagtccct tcccgcttca gtgacaacgt cgagcacagc tgcgcaagga
     6481 acgcccgtcg tggccagcca cgatagccgc gctgcctcgt cctgcagttc attcagggca
     6541 ccggacaggt cggtcttgac aaaaagaacc gggcgcccct gcgctgacag ccggaacacg
     6601 gcggcatcag agcagccgat tgtctgttgt gcccagtcat agccgaatag cctctccacc
     6661 caagcggccg gagaacctgc gtgcaatcca tcttgttcaa tggccgatcc catattggct
     6721 gcaggtcgaa aggcccggag atgaggaaga ggagaacagc gcggcagacg tgcgcttttg
     6781 aagcgtgcag aatgccgggc ctccggagga ccttcgggcg cccgccccgc ccctgagccc
     6841 gcccctgagc ccgcccccgg acccacccct tcccagcctc tgagcccaga aagcgaagga
     6901 gcaaagctgc tattggccgc tgccccaaag gcctacccgc ttccattgct cagcggtgct
     6961 gtccatctgc acgagactag tgagacgtgc tacttccatt tgtcacgtcc tgcacgacgc
     7021 gagctgcggg gcggggggga acttcctgac taggggagga gtagaaggtg gcgcgaaggg
     7081 gccaccaaag aacggagccg gttggcgcct accggtggat gtggaatgtg tgcgaggcca
     7141 gaggccactt gtgtagcgcc aagtgcccag cggggctgct aaagcgcatg ctccagactg
     7201 ccttgggaaa agcgcctccc ctacccggta gaattggccg cgaagttcct atactttcta
     7261 gagaatagga acttcgtcga aaaatcccgg gttaattaag agctccagct tttgttccct
     7321 ttagtgaggg ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa
     7381 ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg
     7441 gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca
     7501 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     7561 tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     7621 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     7681 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     7741 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     7801 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     7861 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     7921 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     7981 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8041 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     8101 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     8161 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     8221 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     8281 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     8341 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     8401 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa
     8461 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta
     8521 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt
     8581 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag
     8641 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca
     8701 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc
     8761 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt
     8821 tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag
     8881 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt
     8941 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat
     9001 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt
     9061 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc
     9121 ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat
     9181 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag
     9241 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt
     9301 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg
     9361 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta
     9421 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc
     9481 gcgcacattt ccccgaaaag tgccac
//
