LOCUS       Exported                8394 bp ds-DNA     circular SYN 20-MAR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8394)
  AUTHORS   Wythe Lab
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Jul 7, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8394
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      909..1497
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             1680..1734
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=5.1"
                     /note="/other=CDS"
     polyA_signal    1736..1870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        complement(1876..2071)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1890..2025
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2078..2117
                     /label=lacI (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=3.7"
                     /note="/other=CDS"
     protein_bind    2130..2151
                     /label=CAP binding site
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     promoter        2166..2196
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2204..2220
                     /label=lac operator
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=100.0"
                     /note="/match_length=100.0"
                     /note="/other=protein_bind"
     primer_bind     2228..2244
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     polyA_signal    2605..2660
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     CDS             complement(2811..3509)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="MVSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
                     AVEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLT
                     ATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMAL
                     KLVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARY
                     CDLPSKLGHR"
     CDS             complement(3510..3569)
                     /codon_start=1
                     /label=Gap43 (fragment)
                     /note="pLannotate"
                     /note="/database=swissprot"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=8.8"
                     /note="/other=CDS"
                     /translation="MLCCMRRTKQVEKNDEDQKI"
     misc_feature    3570..4156
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     promoter        4168..4185
                     /label=SP6 promoter (fragment)
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=True"
                     /note="/identity=100.0"
                     /note="/match_length=94.7"
                     /note="/other=promoter"
     primer_bind     4194..4262
                     /label=oJDW 2416
     protein_bind    4194..4218
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(4248..4577)
                     /codon_start=1
                     /product="degron consisting of residues 1-110 of human 
                     Geminin (Zielke and Edgar, 2015)"
                     /label=Gem1 (1-110)
                     /translation="MNPSMKQKQEEIKENIKNSSVPRRTLKMIQPSASGSLVGRENELS
                     AGLSKRKHRNDHLTSTTSSPGVIVPESSENKNLGGVTQESFDLMIKENPSSQYWKEVAE
                     KRRKAL"
     CDS             complement(4608..5285)
                     /codon_start=1
                     /product="monomeric Azami-Green fluorescent protein 
                     (Karasawa et al., 2003)"
                     /label=mAzami-Green
                     /translation="MVSVIKPEMKIKLCMRGTVNGHNFVIEGEGKGNPYEGTQILDLNV
                     TEGAPLPFAYDILTTVFQYGNRAFTKYPADIQDYFKQTFPEGYHWERSMTYEDQGICTA
                     TSNISMRGDCFFYDIRFDGTNFPPNGPVMQKKTLKWEPSTEKMYVEDGVLKGDVNMRLL
                     LEGGGHYRCDFKTTYKAKKEVRLPDAHKIDHRIEILKHDKDYNKVKLYENAVARYSMLP
                     SQAK"
     CDS             complement(5292..5345)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(5352..5624)
                     /codon_start=1
                     /product="degron consisting of residues 30-120 of human 
                     Cdt1 (Zielke and Edgar, 2015)"
                     /label=Cdt1 (30-120)
                     /translation="PSPARPALRAPASATSGSRKRARPPAAPGRDQARPPARRRLRLSV
                     DEVSSPSTPEAPDIPACPSPGQKIKKSTPAAGQPPHLTSAQDQDTI"
     CDS             complement(5679..6329)
                     /codon_start=1
                     /product="monomeric Kusabira-Orange 2 fluorescent protein"
                     /label=mKO2
                     /translation="MVSVIKPEMKMRYYMDGSVNGHEFTIEGEGTGRPYEGHQEMTLRV
                     TMAEGGPMPFAFDLVSHVFCYGHRVFTKYPEEIPDYFKQAFPEGLSWERSLEFEDGGSA
                     SVSAHISLRGNTFYHKSKFTGVNFPADGPIMQNQSVDWEPSTEKITASDGVLKGDVTMY
                     LKLEGGGNHKCQMKTTYKAAKEILEMPGDHYIGHRLVRKTEGNITEQVEDAVAH"
     primer_bind     complement(6326..6395)
                     /label=oJDW 2415
     promoter        complement(6350..6355)
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     protein_bind    complement(6371..6395)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     intron          complement(6410..6462)
                     /label=rabbit B-globin intron
     intron          complement(6463..7479)
                     /label=chimeric intron
                     /note="pLannotate"
                     /note="/database=snapgene"
                     /note="/fragment=False"
                     /note="/identity=99.9"
                     /note="/match_length=100.0"
                     /note="/other=intron"
     intron          complement(6463..7386)
                     /label=chicken b-actin intron + rabbit b-globin intron
     promoter        complement(7480..7755)
                     /label=chicken beta-actin promoter
     enhancer        7757..8136
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8167..8271
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8272..8394,1..738)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
       61 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      121 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      181 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      241 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
      301 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
      361 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
      421 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
      481 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
      541 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
      601 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
      661 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
      721 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
      781 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
      841 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
      901 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      961 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1021 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1081 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1141 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1201 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1261 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1321 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1381 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     1441 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     1501 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     1561 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     1621 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     1681 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcggatcc
     1741 agacatgata agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa
     1801 atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     1861 taaacaagtt agctttttgc aaaagcctag gcctccaaaa aagcctcctc actacttctg
     1921 gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa ttagtcagcc
     1981 atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     2041 cgggactatg gttgctgact aattgagatg cggatccgct ggcacgacag gtttcccgac
     2101 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
     2161 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
     2221 tttcacacag gaaacagcta tgaccatgat tacgccaagc ttgggctgca ggtcgaggga
     2281 tctccataag agaagaggga cagctatgac tgggagtagt caggagagga ggaaaaatct
     2341 ggctagtaaa acatgtaagg aaaattttag ggatgttaaa gaaaaaaata acacaaaaca
     2401 aaatataaaa aaaatctaac ctcaagtcaa ggcttttcta tggaataagg aatggacagc
     2461 agggggctgt ttcatatact gatgacctct ttatagccaa cctttgttca tggcagccag
     2521 catatgggca tatgttgcca aactctaaac caaatactca ttctgatgtt ttaaatgatt
     2581 tgccctccca tatgtccttc cgagtgagag acacaaaaaa ttccaacaca ctattgcaat
     2641 gaaaataaat ttcctttatt agccagaagt cagatgctca aggggcttca tgatgtcccc
     2701 ataatttttg gcagagggaa aaagatctca gtggtatttg tgagccgggg cattggccac
     2761 accagccacc accttctgat aggcagcctg cacctgagga gtgcggccgc tcatctgtgc
     2821 cccagtttgc tagggaggtc gcagtatctg gccacagcca cctcgtgctg ctcgacgtag
     2881 gtctctttgt cggcctcctt gattctttcc agtcttctgt ccacatagta gacgccgggc
     2941 atcttgaggt tcttagcggg tttcttggat ctgtatgtgg tcttcaagtt gcagatcagg
     3001 tggcccccgc ccacgagctt cagggccatg tcggctctgc cttccaggcc gccgtcagcg
     3061 gggtacaggg tctcggtgga ggcctcccag ccgagtgttt tcttctgcat cacagggccg
     3121 ttggatggga agttcacccc tctgatcttg acgttgtaga tgaggcagcc gtcctggagg
     3181 ctggtgtcct gggtagcggt cagcacgccc ccgtcttcgt atgtggtgac tctctcccat
     3241 gtgaagccct cggggaagga ctgcttaaag aagtcgggga tgccctgggt gtggttgatg
     3301 aaggttttgc tgccgtacat gaagctggta gccaggatgt cgaaggcgaa ggggagaggg
     3361 ccgccctcga ccgccttgat tctcatggtc tgggtgccct cgtagggctt gccttcgccc
     3421 tcggatgtgc acttgaagtg gtggttgttc acggtgccct ccatgtacag cttcatgtgc
     3481 atgttctcct taatcagctc gctcaccatg atcttttggt cctcatcatt cttttcaacc
     3541 tgtttggttc ttctcataca gcacagcatg gttgtggcca tattatcatc gtgtttttca
     3601 aaggaaaacc acgtccccgt ggttcggggg gcctagacgt ttttttaacc tcgactaaac
     3661 acatgtaaag catgtgcacc gaggccccag atcagatccc atacaatggg gtaccttctg
     3721 ggcatccttc agccccttgt tgaatacgct tgaggagagc catttgactc tttccacaac
     3781 tatccaactc acaacgtggc actggggttg tgccgccttt gcaggtgtat cttatacacg
     3841 tggcttttgg ccgcagaggc acctgtcgcc aggtgggggg ttccgctgcc tgcaaagggt
     3901 cgctacagac gttgtttgtc ttcaagaagc ttccagagga actgcttcct tcacgacatt
     3961 caacagacct tgcattcctt tggcgagagg ggaaagaccc ctaggaatgc tcgtcaagaa
     4021 gacagggcca ggtttccggg ccctcacatt gccaaaagac ggcaatatgg tggaaaataa
     4081 catatagaca aacgcacacc ggccttattc caagcggctt cggccagtaa cgttaggggg
     4141 gggggaggga gagggggggg cggaattatt taggtgacac tataggatat cgaaccactt
     4201 tgtacaagaa agctgggttg actggcggcc gctcagtcag atctctacag ggccttccgc
     4261 cgcttctcgg ccacctcttt ccagtactgg ctgctggggt tttctttgat catcaggtcg
     4321 aagctttctt gggtcacgcc gcccaggttc ttgttctcgc tgctctcggg cacgatcacg
     4381 cctgggctag aggtggtgct ggtcaggtgg tcgttccggt gcttccgctt gctcaggccg
     4441 gcagacagct cattctcccg gcccaccaga gatccgctgg cgctgggctg gatcatcttc
     4501 agggtccgcc tgggcacgct gctgttcttg atgttctctt tgatctcttc ctgcttctgc
     4561 ttcatgctgg ggttcatttc cagaggccgc cagtggctgg ggtagccctt ggcctggctg
     4621 ggcagcatgc tgtacctggc cacggcgttc tcgtacagct tcactttgtt gtagtccttg
     4681 tcgtgcttca ggatctcgat ccggtggtcg atcttgtggg cgtcgggcag ccgcacttct
     4741 ttcttggcct tgtaggtggt cttgaagtcg catctgtagt gtccgccgcc ttccagcagc
     4801 agccgcatgt tcacgtcgcc cttcagcacg ccgtcctcca cgtacatctt ctcggtgctg
     4861 ggctcccact tcagggtctt tttctgcatc acggggccgt tgggggggaa gttggtgccg
     4921 tcgaatctga tgtcgtagaa aaagcagtcg cccctcatgc tgatgttgga ggtggcggtg
     4981 cagatgccct ggtcctcgta ggtcatgctc cgctcccagt ggtagccctc ggggaaggtc
     5041 tgcttgaagt agtcctggat gtcggcgggg tacttggtga aggcccggtt gccgtactgg
     5101 aacacggtgg tcaggatgtc gtaggcgaag ggcagagggg cgccttcggt cacgttcagg
     5161 tccaggatct gggtgccctc gtaggggttt cccttgccct cgccctcgat cacgaagttg
     5221 tggccgttca cggttccccg catgcacagc ttgatcttca tctcgggctt gatcacggac
     5281 accatggatc tggggccggg gttctcctcc acgtcgccgc aggtcagcag gctgcccctg
     5341 ccctcgtcga cgatggtgtc ctggtcctgg gcgctggtca ggtgaggagg ctgtccggcg
     5401 gcaggtgtgc tcttcttgat tttctggcca gggctgggac aggcagggat atcgggggcc
     5461 tcgggggtgc tagggctgga cacctcatcc acggacagcc ggagccgtct gcgggcaggg
     5521 ggcctggcct gatctctgcc aggggcggca ggaggtctgg ctctctttct gctgccggag
     5581 gtggcggagg caggggctct cagggcaggt ctggcaggag agggttccag gggcctccaa
     5641 tggctagggt agccgccgag gctaggcagc atgctgtagt gggccacggc atcctccacc
     5701 tgctcggtga tgttgccctc ggttttccgc accagccggt ggccgatgta gtggtcgccg
     5761 ggcatttcca ggatttcttt ggcggccttg taggtggttt tcatctggca cttgtggttt
     5821 ccgccgcctt ccagcttcag gtacatggtc acgtcgccct tcagcacgcc gtcgctggcg
     5881 gtgatcttct cggtgctggg ctcccagtcc acgctctggt tctgcatgat ggggccgtcg
     5941 gcggggaagt tcacgccggt gaacttgctc ttgtggtaga aggtgttgcc ccgcaggctg
     6001 atgtgggcag acacagaggc gctgccgcca tcctcgaatt ccaggctccg ctcccagctc
     6061 aggccctcgg ggaaggcctg cttgaagtag tcggggattt cctcggggta cttggtgaac
     6121 acccggtggc cgtagcagaa cacgtgggac accaggtcga aggcgaaggg catgggtccg
     6181 ccctcggcca tggtgactct cagggtcatt tcctggtggc cctcgtaagg tctgccggtg
     6241 ccctcgccct cgatggtgaa ctcgtggccg ttcacgctgc cgtccatgta gtaccgcatc
     6301 ttcatctcgg gcttgatcac ggacaccatg gatcccaatt cttaattaat cacgaggtac
     6361 cagtcagacc agcctgcttt tttgtacaaa cttgtttgat atctcgagga attctttgcc
     6421 aaaatgatga gacagcacaa taaccagcac gttgcccagg agctgtagga aaaagaagaa
     6481 ggcatgaaca tggttagcag aggctctaga gccgccggtc acacgccaga agccgaaccc
     6541 cgccctgccc cgtccccccc gaaggcagcc gtccccccgc ggacagcccc gaggctggag
     6601 agggagaagg ggacggcggc gcggcgacgc acgaaggccc tccccgccca tttccttcct
     6661 gccggcgccg caccgcttcg ccccgcgccc gctagagggg gtgcggcggc gcctcccaga
     6721 tttcggctcc gcacagattt gggacaaagg aagtccctgc gccctctcgc acgattacca
     6781 taaaaggcaa tggctgcggc tcgccgcgcc tcgacagccg ccggcgctcc gggggccgcc
     6841 gcgcccctcc cccgagccct ccccggcccg aggcggcccc gccccgcccg gcacccccac
     6901 ctgccgccac cccccgcccg gcacggcgag ccccgcgcca cgccccgtac ggagccccgc
     6961 acccgaagcc gggccgtgct cagcaactcg gggagggggg tgcagggggg gttgcagccc
     7021 gaccgacgcg cccacacccc ctgctcaccc ccccacgcac acaccccgca cgcagccttt
     7081 gttcccctcg cagccccccc cgcaccgcgg ggcaccgccc ccggccgcgc tcccctcgcg
     7141 cacactgcgg agcgcacaaa gccccgcgcc gcgcccgcag cgctcacagc cgccgggcag
     7201 cgcggagccg cacgcggcgc tccccacgca cacacacacg cacgcacccc ccgagccgct
     7261 ccccccgcac aaagggccct cccggagccc ctcaaggctt tcacgcagcc acagaaaaga
     7321 aacaagccgt cattaaacca agcgctaatt acagcccgga ggagaagggc cgtcccgccc
     7381 gctcacctgt gggagtaacg cggtcagtca gagccggggc gggcggcgcg aggcggcggc
     7441 ggagcggggc acggggcgaa ggcagcgcgc agcgactccc gcccgccgcg cgcttcgctt
     7501 tttatagggc cgccgccgcc gccgcctcgc cataaaagga aactttcgga gcgcgccgct
     7561 ctgattggct gccgccgcac ctctccgcct cgccccgccc cgcccctcgc cccgccccgc
     7621 cccgcctggc gcgcgccccc cccccccccc gcccccatcg ctgcacaaaa taattaaaaa
     7681 ataaataaat acaaaattgg gggtggggag ggggggagat ggggagagtg aagcagaacg
     7741 tggggctcac ctcgaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     7801 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     7861 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     7921 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     7981 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     8041 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     8101 ttgattacta ttaataacta gtcaataatc aatgtcgacc caggtggcac ttttcgggga
     8161 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8221 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
     8281 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
     8341 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acga
//
