LOCUS       Exported               10601 bp ds-DNA     circular SYN 17-MAY-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10601)
  AUTHORS   Wythe Lab
  TITLE     Direct Submission
  JOURNAL   Exported Tuesday, Jul 15, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10601
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    229..514
                     /label=tyrosinase
     misc_feature    complement(514..1049)
                     /label=Tol2 3' End
     misc_feature    complement(514..530)
                     /label=3' Tol2 ITR
     primer_bind     1061..1077
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1077..1105
                     /label=oJDW 1597 ccdb attR4 Fwd Cold Fusion
     primer_bind     1078..1105
                     /label=oJDW 1372 attR4 + SpeI
     primer_bind     1078..1105
                     /label=oJDW 2074
     protein_bind    1097..1117
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     misc_feature    1104..1117
                     /label=attL4
     enhancer        1119..2125
                     /label=fli1a intron1 enhancer
     misc_feature    1119..1136
                     /label=primer 2 for
     misc_feature    2103..2125
                     /label=primer 2 rev
     promoter        2126..3105
                     /label=fli1a promoter (-977 from exon start)
                     /label=flia promoter
     misc_feature    2126..2148
                     /label=primer 1
     exon            3105..3162
                     /label=Exon 1
     misc_feature    3141..3162
                     /label=primer 1 REV
     protein_bind    3162..3186
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     3163..3236
                     /label=attB1 zFucci FWD
     CDS             3217..3282
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             3283..4011
                     /codon_start=1
                     /label=Cerulean
                     /translation="LRSTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYG
                     KLTLKFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIF
                     FKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQK
                     NGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDH
                     MVLLEFVTAAGITHGMDELYK"
     CDS             4012..4350
                     /codon_start=1
                     /label=zGem
                     /translation="GYPSHWRPLEMSSIRRPKNAENPSENIKKFLVAPTSGAGMGRRTL
                     QVLQPSAVNKNLGRIIENGKAVAKRKMWSAEQVKGSKRVKAEVAVKSTNAENENQPEGV
                     TQEAYEGSG"
     CDS             4351..4407
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4408..5115
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     CDS             5116..5718
                     /codon_start=1
                     /label=zCdt1
                     /translation="GYPSHWRPLEMAQARVTDYFAQSKKAGVSRSLRSKGQKVSGDVVE
                     SAVINKPRSSSRASGSSRKATHSEITTAEPQKQTQLEFLKVIDEALSTETADTVADSRD
                     VKIEGLTASPRTPKRSSPEFDVCSVLFPSTAELHSSAKKRQRLNAGHNCRSSPEERAGQ
                     KTARKKLYLLASDDKVKTTEPLASSSPQAPQQTARKE"
     primer_bind     complement(5695..5774)
                     /label=attB2 zFucci REV
     protein_bind    complement(5746..5770)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    5771..6357
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5938..5955)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6165..6184
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     6358..6380
                     /label=attB1 zFucci FWD
     CDS             6358..6360
                     /codon_start=1
                     /label=MET
                     /translation="M"
     CDS             6361..6411
                     /codon_start=1
                     /label=3X FLAG
                     /translation="DYKDHDGDYKDHDIDYK"
     CDS             6427..6438
                     /codon_start=1
                     /label=MCS
                     /translation="GSTG"
     CDS             6445..6465
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6469..6489
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             6493..6513
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     6520..6564
                     /label=JDW1221-F-BamHI-SpeI-NheI-Kozak-V5-linker-mTagBFP2
     CDS             6535..7245
                     /codon_start=1
                     /label=mTagBFP2
                     /note="Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 
                     Dec "
                     /translation="MVSKGEELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRND
                     MALKLVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAV
                     ARYCDLPSKLGHKLN"
     primer_bind     6535..6564
                     /label=JDW 1196
     CDS             6553..7245
                     /codon_start=1
                     /label=mTagBFP (aa 3-223)
                     /translation="ELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVVE
                     GGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTATQ
                     DTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKLV
                     GGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCDL
                     PSKLGHKLN"
     primer_bind     6572..6595
                     /label=F-mTagBFP2-1
     primer_bind     complement(6659..6680)
                     /label=REV-mTagBFP2-2
     primer_bind     7161..7183
                     /label=F-mTagBFP2-2
     primer_bind     complement(7201..7241)
                     /label=JDW 1233-REV-mTagBFP2-BglIILinker
     primer_bind     complement(7211..7277)
                     /label=R-mTagBFP2-Long
     primer_bind     complement(7211..7241)
                     /label=mTagBFP2-REV-p2A
     primer_bind     complement(7223..7241)
                     /label=REV-mTagBFP2-1
     misc_feature    7242
                     /label=T>C
                     /note="to destroy HindIII site"
     polyA_signal    7302..7436
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    complement(7498..7518)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(7526..7542)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    complement(7526..7542)
                     /label=M13 For -20
     primer_bind     complement(7533..7560)
                     /label=oJDW 1598 ccdb  attR3 Cold Fusion
     primer_bind     complement(7533..7560)
                     
     primer_bind     complement(7533..7560)
                     /label=oJDW xxxxx
     polyA_signal    7558..7692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    7562..7753
                     /label=SV40 late polyA
     misc_feature    7636..7655
                     /label=SV40pA REVSeq
     primer_bind     complement(7780..7813)
                     /label=oJDW 1993
     misc_feature    complement(7814..8330)
                     /label=Tol2 5' end
     misc_feature    complement(8312..8330)
                     /label=5' Tol2 ITR
     primer_bind     complement(8364..8380)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(8417..8435)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8456..8472)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    8480..8496
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8504..8534)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8549..8570
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(8840..9522)
                     /direction=LEFT
                     /label=ColE1 origin
     primer_bind     complement(8849..8871)
                     /label=975
     CDS             complement(9617..10477)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(10478..10582)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ccacctaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
       61 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
      121 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
      181 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacgc tggctaagaa
      241 ctcatcagcc tccccggtcc atctacccac gtaccaatgc accaattggc cacaatgacg
      301 gctactacat ggtgccattc cttcctcttt ataggaatgg agactacctc ctgtccaaca
      361 aggctcttgg atacgagtac gcctacctgt tggacccagg tcattgcaca acaccagaaa
      421 tgccctctga tctgcaaaag acgtgaatat ctgttcagac acccatatcc actctgttcc
      481 acacaggtca gaggtttgtc caggagttct tgacagaggt gtaaaaagta ctcaaaaatt
      541 ttactcaagt gaaagtacaa gtacttaggg aaaattttac tcaattaaaa gtaaaagtat
      601 ctggctagaa tcttacttga gtaaaagtaa aaaagtactc cattaaaatt gtacttgagt
      661 attaaggaag taaaagtaaa agcaagaaag aaaactagag attcttgttt aagcttttaa
      721 tctcaaaaaa cattaaatga aatgcataca aggttttatc ctgctttaga actgtttgta
      781 tttaattatc aaactataag acagacaatc taatgccagt acacgctact caaagttgta
      841 aaacctcaga tttaacttca gtagaagctg attctcaaaa ttgttagtgt caagcctagc
      901 tcttttgggg ctgaaaagca atcctgcagt gctgaaaagc ctctcacagg cagccgatgc
      961 gggaagaggt gtattagtct tgatagagag gctgcaaata gcaggaaacg tgagcagaga
     1021 ctccctggtg tctgaaacac aggccagatg ggccctcgag caggaaacag ctatgaccat
     1081 gattacgcca agctatcaac tttgtataga aaagttggat ctcatcttga cccataaaca
     1141 tacactaaaa cccaacagtc aactttatca aatgaaatga gtgtaattaa ctcaaaattt
     1201 actgaatgtt gattctactc attcgaaaag agttttaaac tcagtgttga aggtagtgag
     1261 ttcattaaat acctcattac ttcaacttaa ctggagtaag ttcatggtac tcacatagat
     1321 tagtttagct caaatggttt gtagcagtcg gttccctcaa gcagtttgag ttgccttaac
     1381 tttttgggtt ttacagtact cagttggttt gagttcactt cacttattgg gttcaacatg
     1441 tgctcaaatt gcatcgttta atcaaataga ttaagttcac agtactcatt aggattagtt
     1501 tttgaactta aatggtttgt tgcagtcggt ttcctcaaac ggtttgagtt tccataactt
     1561 tatgggtttt tcagtgtagt aaacatatgc atccacagat tataatagta acctgcatgt
     1621 tttttccttc acaatttgac acagatacct cacatgattg acttttaact gtacacttta
     1681 gtcgacaatg agaagggtgt ctcaatctat gggtatttcc tgttccacac aatctggttt
     1741 cctgtcaggt ttccttttcc tcctgaaaca aaagacagga atgccctctg tataaaatag
     1801 cacagcgctt ttaatgggtc tctgtacatc ccgacctcat gacgcatctt ctgcgggaca
     1861 gctcaatcac gatcaagttg gcttatgaaa acagtttttt gtccccctgt taagttaaaa
     1921 gcacggtagc atacatgcat tattcatcat gcagcatatt cagcagtaca aaagatgagc
     1981 tcttgacaca cctcagcgta aaatctctat tccagcgtaa tcctgcggtt ctggatggtt
     2041 aagcgagcct tgcaaacacg gccaatttat agccatgttg aaaatagcag ctttgtgagc
     2101 atttcgacct caatgtacca agaatgcgtg ctcggtaact gaaaaagagg gggtggagat
     2161 tttggattta gagatggaat cacatgcacg ctgtatatgc tatataaaaa tagcaggcat
     2221 ttaatgcatg caaaatatgt ttcgacacta aacactacaa gtcggaaata tatctatttt
     2281 tcttttttta acgaaatgag acatttaatg cgcagtaaat gtagtctaac acgcacaata
     2341 ttcctattat tgtccatgaa aaatgtgagg tctatgctac tgatttaaag aaaatgttga
     2401 acaggcacag cacacaagag gtatcagcct ctctatatgg tccatacagc accaggctcg
     2461 ctgcgttcat gtgcattcag aggcgcgcac acgtgtggag cagacagact cgacgcagcg
     2521 ggctttgctt tttattcatg gaagcccatt ttagaagaag ctcgggcaga ctcgtcacat
     2581 tcttcacttc accataggta gatcaaatca aagttattgg atataacgga acaatattca
     2641 tcatattatg agattattat tagaccgagt gcattagaca tatttgtatt cgatcgttga
     2701 aaaagtatat attttatttt agccttaaac tatggttagc ctatattaat cagcataatc
     2761 tgttatttca gacgttgtat taatgattat ttctagcaca gctttgatgc atcaatgcaa
     2821 tgtttctaca aacccgcctt gtttaatgtc ggaaaaaaag cgcaacaaat gtaaagagtt
     2881 ggcaggcatt ctgtaaatat ttccaggcaa aaacatgcac aaaggaaaga aacaccattg
     2941 ttttggtgtc ttatcttcgc tggacaaata tcctgctttg ggtgccgtaa tgaaggcagg
     3001 gccagtgaca cacacacata cacacacaca catacacgca tacacggctt ccttctcaag
     3061 cgcagtgact tcacttccca aatttagagg aaaaaaaacc atccggcgaa actgtctctg
     3121 tctctccgcc acatatcggg ggctggaatt aattcagacg cgcaagtttg tacaaaaaag
     3181 caggctactg tcaagcttca gtcaggatcc ggtatggact acaaagacca tgacggtgat
     3241 tataaagatc atgacatcga ctacaaggat gacgatgaca agctcagatc caccatggtg
     3301 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     3361 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     3421 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     3481 accaccctga cctggggcgt gcagtgcttc gcccgctacc ccgaccacat gaagcagcac
     3541 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     3601 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     3661 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     3721 gagtacaacg ccatcagcga caacgtctat atcaccgccg acaagcagaa gaacggcatc
     3781 aaggccaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     3841 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     3901 agcacccagt ccaagctgtc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
     3961 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa aggatatcca
     4021 tcacactggc ggccgctcga gatgagttcc atcagaagac caaagaatgc agaaaacccg
     4081 tctgaaaaca tcaagaaatt cctggtagcg ccaacatctg gagcaggaat ggggaggagg
     4141 acgcttcagg tgcttcagcc ctctgctgta aacaaaaacc ttggtcgtat aattgagaat
     4201 ggtaaagcag tggccaaaag gaagatgtgg agcgccgagc aagttaaggg ttcaaagaga
     4261 gtcaaagcag aggtggctgt caaatccaca aatgcagaaa atgaaaacca gcctgagggg
     4321 gtcacacaag aggcctatga gggatctgga gccacgaact tctctctgtt aaagcaagca
     4381 ggagacgtgg aagaaaaccc cggtcctatg gtgagcaagg gcgaggagga taacatggcc
     4441 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
     4501 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg
     4561 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
     4621 tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc
     4681 ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc
     4741 gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc
     4801 accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc
     4861 tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag
     4921 ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc
     4981 gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag
     5041 gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg
     5101 gacgagctgt acaagggata tccatcacac tggcggccgc tcgagatggc tcaagctcgt
     5161 gttactgatt attttgcaca gagtaaaaaa gctggtgttt ctcgatcttt acggtctaag
     5221 ggacaaaagg tgtccgggga tgtagtagaa tcagcggtga tcaataaacc gaggagttcg
     5281 agcagagcgt ccggttcttc acgcaaagcg acgcacagtg agatcaccac agcagagccg
     5341 cagaaacaaa ctcagctgga gtttctcaaa gtcatcgacg aggctttatc gacggagact
     5401 gcagataccg tcgcggattc gcgtgatgtg aagattgagg gcttaacggc gagtcccagg
     5461 acccctaagc ggagttcacc ggagttcgac gtgtgttcgg tgttgtttcc gtccacagcc
     5521 gagctgcata gcagcgccaa gaagcggcag agactcaacg cgggccacaa ctgcaggagc
     5581 tcaccggagg agcgcgctgg acagaaaact gcgaggaaaa aactctatct actggcaagc
     5641 gacgacaaag taaagaccac tgagcccttg gccagcagca gccctcaggc acctcaacaa
     5701 actgcccgca aagagtaaac cggtacctga gtcatcgatc agactaccca gctttcttgt
     5761 acaaagtggc cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat
     5821 aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg
     5881 tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc
     5941 tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt
     6001 cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg
     6061 acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac
     6121 cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg
     6181 tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg
     6241 ggcctcggta cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc
     6301 cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg
     6361 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
     6421 gacaaaggat ccaccggtga attcccgaag aagaaaagaa aagtaggacc caagaagaaa
     6481 cgcaaagtcg gaccgaagaa gaaacgaaaa gtcagatctg gcggtgctgg tggtatggtg
     6541 tctaagggcg aagagctgat taaggagaac atgcacatga agctgtacat ggagggcacc
     6601 gtggacaacc atcacttcaa gtgcacatcc gagggcgaag gcaagcccta cgagggcacc
     6661 cagaccatga gaatcaaggt ggtcgagggc ggccctctcc ccttcgcctt cgacatcctg
     6721 gctactagct tcctctacgg cagcaagacc ttcatcaacc acacccaggg catccccgac
     6781 ttcttcaagc agtccttccc tgagggcttc acatgggaga gagtcaccac atacgaagac
     6841 gggggcgtgc tgaccgctac ccaggacacc agcctccagg acggctgcct catctacaac
     6901 gtcaagatca gaggggtgaa cttcacatcc aacggccctg tgatgcagaa gaaaacactc
     6961 ggctgggagg ccttcaccga gacgctgtac cccgctgacg gcggcctgga aggcagaaac
     7021 gacatggccc tgaagctcgt gggcgggagc catctgatcg caaacgccaa gaccacatat
     7081 agatccaaga aacccgctaa gaacctcaag atgcctggcg tctactatgt ggactacaga
     7141 ctggaaagaa tcaaggaggc caacaacgag acctacgtcg agcagcacga ggtggcagtg
     7201 gccagatact gcgacctccc tagcaaactg gggcacaagc tcaatgctag cggctgatat
     7261 ctataatgag gcgcgccgtc gacacgcgtt aatgatacgt agatccagac atgataagat
     7321 acattgatga gtttggacaa accacaacta gaatgcagtg aaaaaaatgc tttatttgtg
     7381 aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa caagttaaca
     7441 acatctagac attcatttta tgtttcaggt tcagggggag gtgtgggagg ttttttccaa
     7501 ctttattata catagttgat aattcactgg ccgtcgtttt acggtaccat cgatgatgat
     7561 ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat gcagtgaaaa
     7621 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     7681 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggaggtg
     7741 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtatggctga ttatgatcct
     7801 ctagatcaga tctgcgaaga tacggccacg ggtgctcttg atcctgtggc tgattttgga
     7861 ctgtgctgct cgcagctgct gatgaatcac atacttcctc cattttcttc cactgattga
     7921 ctgttataat ttccctaatt tccaggtcaa ggtgctgtgc attgtggtaa tagatgtgac
     7981 atgacgtcac ttccaaagga ccaatgaaca tgtctgacca atttcatata atgtgaaaac
     8041 gattttcata ggcagaataa ataacattta aattaaactg ggcatcagcg caattcaatt
     8101 ggtttggtaa tagcaaggga aaatagaatg aagtgatctc caaaaaataa gtactttttg
     8161 actgtaaata aaattgtaag gagtaaaaag tacttttttt tctaaaaaaa tgtaattaag
     8221 taaaagtaaa agtattgatt tttaattgta ctcaagtaaa gtaaaaatcc ccaaaaataa
     8281 tacttaagta cagtaatcaa gtaaaattac tcaagtactt tacacctctg gttcttgacc
     8341 ccctaccttc agcaagccca gcagatccac tagttctaga gcggccgcca ccgcggtgga
     8401 gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat
     8461 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8521 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8581 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8641 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8701 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8761 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8821 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8881 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8941 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     9001 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     9061 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9121 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9181 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9241 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     9301 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9361 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9421 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9481 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9541 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9601 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9661 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9721 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9781 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9841 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9901 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9961 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    10021 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    10081 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10141 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10201 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10261 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10321 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10381 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10441 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10501 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10561 ataaacaaat aggggttccg cgcacatttc cccgaaaagt g
//
