LOCUS       Exported                9169 bp ds-DNA     linear   SYN 11-JUL-2025
DEFINITION  pAT031 - lenti 
            pEF-MCP-MCP-3xFLAG-LibClone-site-T2A-mtagBFP2-P2A-BSD-WPRE-.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9169)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 11, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..9169
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
       61 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      121 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      181 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      241 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      301 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      361 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      421 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      481 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      541 agtaagacca ccgcacagca agcggccggc cgcgctgatc ttcagacctg gaggaggaga
      601 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
      661 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
      721 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
      781 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
      841 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
      901 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
      961 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1021 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     1081 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     1141 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     1201 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     1261 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     1321 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     1381 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     1441 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     1501 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     1561 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     1621 gcagagatcc agtttggtta gtaccgggcc cgctctagag atcccatttg actcaggcgg
     1681 ccgcgtggat aaccgtatta ccgccatgca tgtgcccgtc agtgggcaga gcgcacatcg
     1741 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     1801 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     1861 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     1921 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
     1981 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
     2041 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
     2101 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
     2161 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     2221 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
     2281 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     2341 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     2401 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     2461 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     2521 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     2581 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     2641 cgggcgccgt ccaggcacct cgattagttc tcgcgctttt ggagtacgtc gtctttaggt
     2701 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     2761 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     2821 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     2881 tgagctagcc gttaaaatgg cttctaactt tactcagttc gttctcgtcg acaatggcgg
     2941 aactggcgac gtgactgtcg ccccaagcaa cttcgctaac gggatcgctg aatggatcag
     3001 ctctaactcg cgttcacagg cttacaaagt aacctgtagc gttcgtcaga gctctgcgca
     3061 gaatcgcaaa tacaccatca aagtcgaggt gcctaaaggc gcctggcgtt cgtacttaaa
     3121 tatggaacta accattccaa ttttcgccac gaattccgac tgcgagctta ttgttaaggc
     3181 aatgcaaggt ctcctaaaag atggaaaccc gattccctca gcaatcgcag caaactccgg
     3241 catctacgcg atctacgcca tggcttctat ggcttctaac tttactcagt tcgttctcgt
     3301 cgacaatggc ggaactggcg acgtgactgt cgccccaagc aacttcgcta acgggatcgc
     3361 tgaatggatc agctctaact cgcgttcaca ggcttacaaa gtaacctgta gcgttcgtca
     3421 gagctctgcg cagaatcgca aatacaccat caaagtcgag gtgcctaaag gcgcctggcg
     3481 ttcgtactta aatatggaac taaccattcc aattttcgcc acgaattccg actgcgagct
     3541 tattgttaag gcaatgcaag gtctcctaaa agatggaaac ccgattccct cagcaatcgc
     3601 agcaaactcc ggcatctacg cgatcgacta caaagaccat gacggtgatt ataaagatca
     3661 tgacatcgac tacaaggatg acgatgacaa gggtggatcc ggatcaggct ccggagacgg
     3721 gtcaagcgtc tctggatctg gagagggaag gggaagcctc ctaacttgcg gagatgtcga
     3781 ggagaatcct ggcccaatgg ttagtaaggg agaggagttg ataaaagaga atatgcacat
     3841 gaaactgtat atggaaggga cagtggacaa ccatcacttc aagtgcacct ctgagggaga
     3901 aggaaagcca tacgaaggca cccagactat gcgaattaag gtcgtggagg gagggcctct
     3961 ccccttcgcc ttcgatattc ttgcaacatc tttcctctac gggagcaaaa ccttcatcaa
     4021 tcacacacaa ggcatacctg acttttttaa gcagagtttt ccagagggct ttacctggga
     4081 gcgtgtgact acatacgaag atgggggtgt gctgacggct acacaggata cttcacttca
     4141 ggacggttgt ctgatctaca acgtcaagat caggggggtc aatttcacct cgaatggacc
     4201 agtaatgcaa aaaaagaccc tgggatggga agcttttact gaaactctct atcccgccga
     4261 cggcggcctc gaaggcagaa acgatatggc tttgaagctg gttggcgggt cccatctaat
     4321 tgccaatgcc aaaaccacat ataggagcaa aaagcctgca aagaacctga agatgccggg
     4381 tgtgtactat gttgactata gattagagcg gatcaaagag gcaaataacg aaacgtacgt
     4441 ggagcagcac gaggtagccg tggcgcgcta ctgcgattta ccctccaaac tgggccataa
     4501 actgaactgt acaggaagcg gagctactaa cttcagcctg ctgaagcagg ctggcgacgt
     4561 ggaagaaaac ccaggaccta tggccaagcc tttgtctcaa gaagaatcca ccctcattga
     4621 aagagcaacg gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc
     4681 agctctctct agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg
     4741 accttgtgca gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac
     4801 ttgtatcgtc gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg
     4861 acaggtgctt ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca
     4921 gccgacggca gttgggattc gtgaattgct gccctctggt tatgtgtggg agggctaaga
     4981 attcgtcgag ggacctaata acttcgtata gcatacatta tacgaagtta tacatgttta
     5041 agggttccgg ttccactagg tacaattcga tatcaagctt atcgataatc aacctctgga
     5101 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     5161 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     5221 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     5281 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     5341 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     5401 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     5461 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     5521 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     5581 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     5641 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgatcgag
     5701 acctagaaaa acatggagca atcacaagta gcaatacagc agctaccaat gctgattgtg
     5761 cctggctaga agcacaagag gaggaggagg tgggttttcc agtcacacct caggtacctt
     5821 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     5881 gactggaagg gctaattcac tcccaacgaa gacaagatat ccttgatctg tggatctacc
     5941 acacacaagg ctacttccct gattggcaga actacacacc agggccaggg atcagatatc
     6001 cactgacctt tggatggtgc tacaagctag taccagttga gcaagagaag gtagaagaag
     6061 ccaatgaagg agagaacacc cgcttgttac accctgtgag cctgcatggg atggatgacc
     6121 cggagagaga agtattagag tggaggtttg acagccgcct agcatttcat cacatggccc
     6181 gagagctgca tccggactgt actgggtctc tctggttaga ccagatctga gcctgggagc
     6241 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     6301 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     6361 agtcagtgtg gaaaatctct agcagcatgt gagcaaaagg ccagcaaaag gccaggaacc
     6421 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     6481 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     6541 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     6601 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     6661 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6721 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6781 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6841 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6901 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6961 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     7021 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     7081 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     7141 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     7201 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     7261 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     7321 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     7381 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     7441 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     7501 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     7561 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     7621 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     7681 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7741 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7801 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7861 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7921 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7981 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     8041 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     8101 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     8161 gggttccgcg cacatttccc cgaaaagtgc cacctgacgc ttaagcggtc gacggatcgg
     8221 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     8281 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     8341 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     8401 gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt gattattgac
     8461 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     8521 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     8581 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     8641 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     8701 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     8761 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     8821 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     8881 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     8941 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     9001 acggtgggag gtctatataa gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc
     9061 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     9121 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactc
//
