LOCUS       Exported               10241 bp ds-DNA     linear   SYN 11-JUL-2025
DEFINITION  pAT122 
            AAVS1-PuroR-9xTet0-pEF-IGKleader-Cas9site-hIgG1_FC-Myc-PDGFRB-T2A-Ci
            trine-24xMS2-PolyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10241)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 11, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10241
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
       61 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
      121 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
      181 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
      241 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
      301 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
      361 aacaatttca cacaggaaac agctatgacc atgattacgc caagctcaga attaaccctc
      421 actaaaggga ctagtcctgc aggtttaaac caattggccc tttgctttct ctgaccagca
      481 ttctctcccc tgggcctgtg ccgctttctg tctgcagctt gtggcctggg tcacctctac
      541 ggctggccca gatccttccc tgccgcctcc ttcaggttcc gtcttcctcc actccctctt
      601 ccccttgctc tctgctgtgt tgctgcccaa ggatgctctt tccggagcac ttccttctcg
      661 gcgctgcacc acgtgatgtc ctctgagcgg atcctccccg tgtctgggtc ctctccgggc
      721 atctctcctc cctcacccaa ccccatgccg tcttcactcg ctgggttccc ttttccttct
      781 ccttctgggg cctgtgccat ctctcgtttc ttaggatggc cttctccgac ggatgtctcc
      841 cttgcgtccc gcctcccctt cttgtaggcc tgcatcatca ccgtttttct ggacaacccc
      901 aaagtacccc gtctccctgg ctttagccac ctctccatcc tcttgctttc tttgcctgga
      961 caccccgttc tcctgtggat tcgggtcacc tctcactcct ttcatttggg cagctcccct
     1021 acccccctta cctctctagt ctgtgctagc tcttccagcc ccctgtcatg gcatcttcca
     1081 ggggtccgag agctcagcta gtcttcttcc tccaacccgg gcccctatgt ccacttcagg
     1141 acagcatgtt tgctgcctcc agggatcctg tgtccccgag ctgggaccac cttatattcc
     1201 cagggccggt taatgtggct ctggttctgg gtacttttat ctgtcccctc caccccacag
     1261 tggggcaagc ttctgacctc ttctcttcct cccacagggc ctcgagagat caggcagcgg
     1321 agagggcaga ggaagtcttc taacatgcgg tgacgtggag gagaatcccg gccctaggct
     1381 cgagatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     1441 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     1501 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     1561 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     1621 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     1681 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     1741 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     1801 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     1861 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     1921 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     1981 ctgatctaga gggcccgttt aaacccgctg atcagcctcg actgtgcctt ctagttgcca
     2041 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     2101 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     2161 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     2221 tgctggggat gcggtgggct ctatggcttc tgaggcggaa gaaccagctg ggggctagca
     2281 aagcctagga acagctgcat atagcactgc agtttactcc ctatcagtga tagagagtca
     2341 aaaggccatg ccctccctat cagtgataga gagagcagac tcaaggtggt ccctatcagt
     2401 gatagagaga ttgagcaaga gggcctccct atcagtgata gagaatagca ctgcagttta
     2461 ctccctatca gtgatagaga gtcaaaaggc catgccctcc ctatcagtga tagagagagc
     2521 agactcaagg tggtccctat cagtgataga gagattgagc aagagggcct ccctatcagt
     2581 gatagagatt gatccagtca gggcctccct atcagtgata gagaagctat gtgcaggtag
     2641 ggctgtacag tgggaatgca tgagcgcaca tcgcccacag tccccgagaa gttgggggga
     2701 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     2761 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     2821 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
     2881 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
     2941 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
     3001 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
     3061 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     3121 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     3181 gatagtcttg taaatgcggg ccaacatgtg cacactggta tttcggtttt tggggccgcg
     3241 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     3301 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     3361 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     3421 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     3481 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     3541 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     3601 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     3661 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     3721 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     3781 gttcaaagtt tttttcttcc atttcaggtg tcgtgagcta gcgattacgc caagctcgaa
     3841 attaaccctc actaaaggga acaaaagctg gagctcccac cgcggtgggc caccatggag
     3901 acagacacac tcctgctatg ggtactgctg ctctgggttc caggttccac tggtgacgct
     3961 agcgagacgg gcggatcagg cggtcgtctc ggatccgaca aaactcacac atgcccaccg
     4021 tgcccagcac ctgaactcct ggggggaccg tcagtcttcc tcttcccccc aaaacccaag
     4081 gacaccctca tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac
     4141 gaagaccctg aggtcaagtt caactggtac gtggacggcg tggaggtgca taatgccaag
     4201 acaaagccgc gggaggagca gtacaacagc acgtaccgtg tggtcagcgt cctcaccgtc
     4261 ctgcaccagg actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc
     4321 ccagccccca tcgagaaaac catctccaaa gccaaagggc agccccgaga accacaggtc
     4381 tacaccctgc ccccatcccg ggaggagatg accaagaacc aggtcagcct gacctgcctg
     4441 gtcaaaggct tctatcccag cgacatcgcc gtggagtggg agagcaatgg gcagccggag
     4501 aacaactaca agaccacgcc tcccgtgctg gactccgacg gctccttctt cctctacagc
     4561 aagctcaccg tggacaagag caggtggcag caggggaacg tcttctcatg ctccgtgatg
     4621 cacgaggctc tgcacaacca ctacacgcag aagagcctct ccctgtctcc gggtaaagga
     4681 ggaggtggaa gcggaggagg aggaagcgga ggaggaggta gcggagaaca aaaactcatc
     4741 tcagaagagg atctgaatgc tgtgggccag gacacgcagg aggtcatcgt ggtgccacac
     4801 tccttgccct ttaaggtggt ggtgatctca gccatcctgg ccctggtggt gctcaccatc
     4861 atctccctta tcatcctcat catgctttgg cagaagaagc cacgttgtac aggcagtgga
     4921 gagggcagag gaagtctgct aacatgcggt gacgtcgagg agaatcctgg cccaccggtc
     4981 gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     5041 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     5101 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     5161 cccaccctcg tgaccacctt cggctacggc ctgatgtgct tcgcccgcta ccccgaccac
     5221 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     5281 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     5341 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     5401 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     5461 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     5521 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     5581 aaccactacc tgagctacca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     5641 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     5701 aagccaaaaa agaagagaaa ggtatagttg cggccgcgaa ttcgcccttc gcgggatcct
     5761 acggtactta ttgccaagaa agcacgagca tcagccgtgc ctccaggtcg aatcttcaaa
     5821 cgacgacgat cacgcgtcgc tccagtattc cagggttcat cagatcctac ggtacttatt
     5881 gccaagaaag cacgagcatc agccgtgcct ccaggtcgaa tcttcaaacg acgacgatca
     5941 cgcgtcgctc cagtattcca gggttcatca gatcctacgg tacttattgc caagaaagca
     6001 cgagcatcag ccgtgcctcc aggtcgaatc ttcaaacgac gacgatcacg cgtcgctcca
     6061 gtattccagg gttcatcaga tcctacggta cttattgcca agaaagcacg agcatcagcc
     6121 gtgcctccag gtcgaatctt caaacgacga cgatcacgcg tcgctccagt attccagggt
     6181 tcatcagatc ctacggtact tattgccaag aaagcacgag catcagccgt gcctccaggt
     6241 cgaatcttca aacgacgacg atcacgcgtc gctccagtat tccagggttc atcagatcct
     6301 acggtactta ttgccaagaa agcacgagca tcagccgtgc ctccaggtcg aatcttcaaa
     6361 cgacgacgat cacgcgtcgc tccagtattc cagggttcat cagatcctac ggtacttatt
     6421 gccaagaaag cacgagcatc agccgtgcct ccaggtcgaa tcttcaaacg acgacgatca
     6481 cgcgtcgctc cagtattcca gggttcatca gatcctacgg tacttattgc caagaaagca
     6541 cgagcatcag ccgtgcctcc aggtcgaatc ttcaaacgac gacgatcacg cgtcgctcca
     6601 gtattccagg gttcatcaga tcctacggta cttattgcca agaaagcacg agcatcagcc
     6661 gtgcctccag gtcgaatctt caaacgacga cgatcacgcg tcgctccagt attccagggt
     6721 tcatcagatc ctacggtact tattgccaag aaagcacgag catcagccgt gcctccaggt
     6781 cgaatcttca aacgacgacg atcacgcgtc gctccagtat tccagggttc atcagatcct
     6841 acggtactta ttgccaagaa agcacgagca tcagccgtgc ctccaggtcg aatcttcaaa
     6901 cgacgacgat cacgcgtcgc tccagtattc cagggttcat cagatcctac ggtacttatt
     6961 gccaagaaag cacgagcatc agccgtgcct ccaggtcgaa tcttcaaacg acgacgatca
     7021 cgcgtcgctc cagtattcca gggttcatca gatctaacta aacttgttta ttgcagctta
     7081 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7141 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct gcatatgtaa
     7201 attgtaagcg tcatatgtaa attgtaagcg tctcggcctc tagagtcgag atgacattga
     7261 ttattgaact agggacagga ttggtgacag aaaagcccca tccttaggcc tcctccttcc
     7321 tagtctcctg atattgggtc taacccccac ctcctgttag gcagattcct tatctggtga
     7381 cacaccccca tttcctggag ccatctctct ccttgccaga acctctaagg tttgcttacg
     7441 atggagccag agaggatcct gggagggaga gcttggcagg gggtgggagg gaaggggggg
     7501 atgcgtgacc tgcccggttc tcagtggcca ccctgcgcta ccctctccca gaacctgagc
     7561 tgctctgacg cggctgtctg gtgcgtttca ctgatcctgg tgctgcagct tccttacact
     7621 tcccaagagg agaagcagtt tggaaaaaca aaatcagaat aagttggtcc tgagttctaa
     7681 ctttggctct tcacctttct agtccccaat ttatattgtt cctccgtgcg tcagttttac
     7741 ctgtgagata aggccagtag ccagccccgt cctggcaggg ctgtggtgag gaggggggtg
     7801 tccgtgtgga aaactccctt tgtgagaatg gtgcgtccta ggtgttcacc aggtcgtggc
     7861 cgcctctact ccctttctct ttctccatcc ttctttcctt aaagagtccc cagtgctatc
     7921 tgggacatat tcctccgccc agagcagggt cccgcttccc taaggccctg ctctgggctt
     7981 ctgggtttga gtccttggca agcccaggag aggcgctcag gcttccctgt cccccttcct
     8041 cgtccaccat ctcatgcccc tggctctcct gccccttccc tacaggggtt cctggctctg
     8101 ctcttcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt
     8161 attttctcct tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga
     8221 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     8281 atgagattat caaaaaggat cttcacctct tgacgagttc ttctgaatta ttaacgctta
     8341 caatttcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatcag
     8401 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     8461 caaatatgta tccgctcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     8521 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     8581 aatgtagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     8641 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     8701 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     8761 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     8821 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     8881 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     8941 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     9001 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     9061 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     9121 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     9181 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     9241 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     9301 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     9361 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     9421 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     9481 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     9541 aagcatttat cagggttatt gtctcatgac caaaatccct taacgtgagt tttcgttcca
     9601 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     9661 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     9721 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     9781 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     9841 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     9901 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     9961 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    10021 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
    10081 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    10141 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    10201 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac g
//
