LOCUS       Exported                8996 bp ds-DNA     linear   SYN 11-JUL-2025
DEFINITION  pAT209 
            AAVS1-PuroR-9xTet0-pEF-MS2x7_equi-IGKleader-Cas9site-hIgG1_FC-Myc-PD
            GFRB-T2A-Citrine-PolyA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8996)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 11, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..8996
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctca gaattaaccc
     1021 tcactaaagg gactagtcct gcaggtttaa accaattggc cctttgcttt ctctgaccag
     1081 cattctctcc cctgggcctg tgccgctttc tgtctgcagc ttgtggcctg ggtcacctct
     1141 acggctggcc cagatccttc cctgccgcct ccttcaggtt ccgtcttcct ccactccctc
     1201 ttccccttgc tctctgctgt gttgctgccc aaggatgctc tttccggagc acttccttct
     1261 cggcgctgca ccacgtgatg tcctctgagc ggatcctccc cgtgtctggg tcctctccgg
     1321 gcatctctcc tccctcaccc aaccccatgc cgtcttcact cgctgggttc ccttttcctt
     1381 ctccttctgg ggcctgtgcc atctctcgtt tcttaggatg gccttctccg acggatgtct
     1441 cccttgcgtc ccgcctcccc ttcttgtagg cctgcatcat caccgttttt ctggacaacc
     1501 ccaaagtacc ccgtctccct ggctttagcc acctctccat cctcttgctt tctttgcctg
     1561 gacaccccgt tctcctgtgg attcgggtca cctctcactc ctttcatttg ggcagctccc
     1621 ctacccccct tacctctcta gtctgtgcta gctcttccag ccccctgtca tggcatcttc
     1681 caggggtccg agagctcagc tagtcttctt cctccaaccc gggcccctat gtccacttca
     1741 ggacagcatg tttgctgcct ccagggatcc tgtgtccccg agctgggacc accttatatt
     1801 cccagggccg gttaatgtgg ctctggttct gggtactttt atctgtcccc tccaccccac
     1861 agtggggcaa gcttctgacc tcttctcttc ctcccacagg gcctcgagag atcaggcagc
     1921 ggagagggca gaggaagtct tctaacatgc ggtgacgtgg aggagaatcc cggccctagg
     1981 ctcgagatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga cgtccccagg
     2041 gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat
     2101 ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg
     2161 ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt ctggaccacg
     2221 ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg
     2281 agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc gcaccggccc
     2341 aaggagcccg cgtggttcct ggccaccgtc ggcgtctcgc ccgaccacca gggcaagggt
     2401 ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc
     2461 ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg cttcaccgtc
     2521 accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg caagcccggt
     2581 gcctgatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
     2641 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
     2701 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
     2761 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
     2821 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctgggggcta
     2881 gcaaagccta ggaacagctg catatagcac tgcagtttac tccctatcag tgatagagag
     2941 tcaaaaggcc atgccctccc tatcagtgat agagagagca gactcaaggt ggtccctatc
     3001 agtgatagag agattgagca agagggcctc cctatcagtg atagagaata gcactgcagt
     3061 ttactcccta tcagtgatag agagtcaaaa ggccatgccc tccctatcag tgatagagag
     3121 agcagactca aggtggtccc tatcagtgat agagagattg agcaagaggg cctccctatc
     3181 agtgatagag attgatccag tcagggcctc cctatcagtg atagagaagc tatgtgcagg
     3241 tagggctgta cagtgggaat gcatgagcgc acatcgccca cagtccccga gaagttgggg
     3301 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     3361 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     3421 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     3481 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     3541 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     3601 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     3661 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     3721 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     3781 caagatagtc ttgtaaatgc gggccaacat gtgcacactg gtatttcggt ttttggggcc
     3841 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3901 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3961 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     4021 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     4081 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     4141 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     4201 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     4261 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     4321 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     4381 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag ctagcgatta cgccaagctc
     4441 gaaattaacc ctcactaaag ggaacaaaag ctggagctcc caccgcggtg ggctccgttt
     4501 atgctttcca gctcggtatc agttacgtag acgctgcacg agcatcagcc gtgccttaac
     4561 aaggctaaca aacgactcgc acgagcatca gccgtgcctg agcccttact ccgtggtctc
     4621 gcacgagcat cagccgtgcc tatcatacaa ttccactgta tttgcacgag catcagccgt
     4681 gcctggccat taacacccaa acagtggcac gagcatcagc cgtgcctcag gcctatgttc
     4741 cagacggatg cacgagcatc agccgtgcct agaggcgagt cctgcgcgaa tcgcacgagc
     4801 atcagccgtg cctgaaccga agtccttgga gtttagagcg agggccgctc gactagctac
     4861 gtagatctgc caccatggag acagacacac tcctgctatg ggtactgctg ctctgggttc
     4921 caggttccac tggtgacgct agcgagacgg gcggatcagg cggtcgtctc ggatccgaca
     4981 aaactcacac atgcccaccg tgcccagcac ctgaactcct ggggggaccg tcagtcttcc
     5041 tcttcccccc aaaacccaag gacaccctca tgatctcccg gacccctgag gtcacatgcg
     5101 tggtggtgga cgtgagccac gaagaccctg aggtcaagtt caactggtac gtggacggcg
     5161 tggaggtgca taatgccaag acaaagccgc gggaggagca gtacaacagc acgtaccgtg
     5221 tggtcagcgt cctcaccgtc ctgcaccagg actggctgaa tggcaaggag tacaagtgca
     5281 aggtctccaa caaagccctc ccagccccca tcgagaaaac catctccaaa gccaaagggc
     5341 agccccgaga accacaggtc tacaccctgc ccccatcccg ggaggagatg accaagaacc
     5401 aggtcagcct gacctgcctg gtcaaaggct tctatcccag cgacatcgcc gtggagtggg
     5461 agagcaatgg gcagccggag aacaactaca agaccacgcc tcccgtgctg gactccgacg
     5521 gctccttctt cctctacagc aagctcaccg tggacaagag caggtggcag caggggaacg
     5581 tcttctcatg ctccgtgatg cacgaggctc tgcacaacca ctacacgcag aagagcctct
     5641 ccctgtctcc gggtaaagga ggaggtggaa gcggaggagg aggaagcgga ggaggaggta
     5701 gcggagaaca aaaactcatc tcagaagagg atctgaatgc tgtgggccag gacacgcagg
     5761 aggtcatcgt ggtgccacac tccttgccct ttaaggtggt ggtgatctca gccatcctgg
     5821 ccctggtggt gctcaccatc atctccctta tcatcctcat catgctttgg cagaagaagc
     5881 cacgttgtac aggcagtgga gagggcagag gaagtctgct aacatgcggt gacgtcgagg
     5941 agaatcctgg cccaccggtc gccaccatgg tgagcaaggg cgaggagctg ttcaccgggg
     6001 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     6061 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     6121 gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc ctgatgtgct
     6181 tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     6241 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     6301 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     6361 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     6421 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     6481 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     6541 gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg agcaaagacc
     6601 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
     6661 tcggcatgga cgagctgtac aagccaaaaa agaagagaaa ggtatagtta actaaacttg
     6721 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     6781 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     6841 gtctgcatat gtaaattgta agcgtctcgg cctctagagt cgagatgaca ttgattattg
     6901 aactagggac aggattggtg acagaaaagc cccatcctta ggcctcctcc ttcctagtct
     6961 cctgatattg ggtctaaccc ccacctcctg ttaggcagat tccttatctg gtgacacacc
     7021 cccatttcct ggagccatct ctctccttgc cagaacctct aaggtttgct tacgatggag
     7081 ccagagagga tcctgggagg gagagcttgg cagggggtgg gagggaaggg ggggatgcgt
     7141 gacctgcccg gttctcagtg gccaccctgc gctaccctct cccagaacct gagctgctct
     7201 gacgcggctg tctggtgcgt ttcactgatc ctggtgctgc agcttcctta cacttcccaa
     7261 gaggagaagc agtttggaaa aacaaaatca gaataagttg gtcctgagtt ctaactttgg
     7321 ctcttcacct ttctagtccc caatttatat tgttcctccg tgcgtcagtt ttacctgtga
     7381 gataaggcca gtagccagcc ccgtcctggc agggctgtgg tgaggagggg ggtgtccgtg
     7441 tggaaaactc cctttgtgag aatggtgcgt cctaggtgtt caccaggtcg tggccgcctc
     7501 tactcccttt ctctttctcc atccttcttt ccttaaagag tccccagtgc tatctgggac
     7561 atattcctcc gcccagagca gggtcccgct tccctaaggc cctgctctgg gcttctgggt
     7621 ttgagtcctt ggcaagccca ggagaggcgc tcaggcttcc ctgtccccct tcctcgtcca
     7681 ccatctcatg cccctggctc tcctgcccct tccctacagg ggttcctggc tctgctcttc
     7741 gccttcttga cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc
     7801 tccttacgca tctgtgcggt atttcacacc gcatcaggtg gcacttttcg gggaaatgtg
     7861 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     7921 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
     7981 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     8041 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     8101 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     8161 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     8221 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     8281 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     8341 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     8401 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     8461 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     8521 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgaatactc aaccaagtca
     8581 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     8641 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     8701 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     8761 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     8821 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     8881 ctttttcaat attattgaag catttatcag ggttattgtc tcatgaccaa aatcccttaa
     8941 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
