LOCUS       Exported               10477 bp ds-DNA     linear   SYN 11-JUL-2025
DEFINITION  pAT124- lenti 
            pEF-DHFR-MCP-MCP-HALO-LibClone-site-T2A-mtagBFP2-P2A-BSD-WPRE-.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10477)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 11, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10477
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
       61 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      121 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      181 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      241 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      301 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      361 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      421 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      481 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      541 agtaagacca ccgcacagca agcggccggc cgcgctgatc ttcagacctg gaggaggaga
      601 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
      661 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
      721 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
      781 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
      841 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
      901 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
      961 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1021 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     1081 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     1141 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     1201 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     1261 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     1321 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     1381 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     1441 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     1501 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     1561 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     1621 gcagagatcc agtttggtta gtaccgggcc cgctctagag atcccatttg actcaggcgg
     1681 ccgcgtggat aaccgtatta ccgccatgca tgtgcccgtc agtgggcaga gcgcacatcg
     1741 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     1801 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     1861 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     1921 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
     1981 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
     2041 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
     2101 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
     2161 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     2221 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
     2281 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     2341 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     2401 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     2461 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     2521 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     2581 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     2641 cgggcgccgt ccaggcacct cgattagttc tcgcgctttt ggagtacgtc gtctttaggt
     2701 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     2761 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     2821 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     2881 tgagctagcc accatgatct cacttattgc tgcactcgca gtcgactacg ttatcgggat
     2941 ggagaacgct atgccatgga atctgcccgc cgacctcgcg tggttcaagc gcaacacatt
     3001 gaacaagccc gttatcatgg gccgacacac ttgggaatcc attggcagac cgctccctgg
     3061 tagaaaaaat atcatactct cctcacagcc ctctactgat gatagagtta catgggtgaa
     3121 atcagtagat gaggcgattg cggcttgtgg cgatgtcccg gaaattatgg ttataggggg
     3181 tgggcgggta attgagcaat ttctccctaa ggcgcagaag ctctacctga cgcacatcga
     3241 cgctgaggta gagggggaca cgcacttccc tgactacgaa cctgacgact gggagtccgt
     3301 gttttctgaa ttccacgacg cggacgcaca gaattcccat tcctactgtt ttgaaatact
     3361 tgaacggcta gccgttaaaa tggcttctaa ctttactcag ttcgttctcg tcgacaatgg
     3421 cggaactggc gacgtgactg tcgccccaag caacttcgct aacgggatcg ctgaatggat
     3481 cagctctaac tcgcgttcac aggcttacaa agtaacctgt agcgttcgtc agagctctgc
     3541 gcagaatcgc aaatacacca tcaaagtcga ggtgcctaaa ggcgcctggc gttcgtactt
     3601 aaatatggaa ctaaccattc caattttcgc cacgaattcc gactgcgagc ttattgttaa
     3661 ggcaatgcaa ggtctcctaa aagatggaaa cccgattccc tcagcaatcg cagcaaactc
     3721 cggcatctac gcgatctacg ccatggcttc tatggcttct aactttactc agttcgttct
     3781 cgtcgacaat ggcggaactg gcgacgtgac tgtcgcccca agcaacttcg ctaacgggat
     3841 cgctgaatgg atcagctcta actcgcgttc acaggcttac aaagtaacct gtagcgttcg
     3901 tcagagctct gcgcagaatc gcaaatacac catcaaagtc gaggtgccta aaggcgcctg
     3961 gcgttcgtac ttaaatatgg aactaaccat tccaattttc gccacgaatt ccgactgcga
     4021 gcttattgtt aaggcaatgc aaggtctcct aaaagatgga aacccgattc cctcagcaat
     4081 cgcagcaaac tccggcatct acgcgatcat ggcagaaatc ggtactggct ttccattcga
     4141 cccccattat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg gtccgcgcga
     4201 tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt ggcgcaacat
     4261 catcccgcat gttgcaccga cccatcgctg cattgctcca gacctgatcg gtatgggcaa
     4321 atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca tggatgcctt
     4381 catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg gctccgctct
     4441 gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat ttatggagtt
     4501 catccgccct atcccgacct gggacgaatg gccagaattt gcccgcgaga ccttccaggc
     4561 cttccgcacc accgacgtcg gccgcaagct gatcatcgat cagaacgttt ttatcgaggg
     4621 tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc attaccgcga
     4681 gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg agctgccaat
     4741 cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact ggctgcacca
     4801 gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc caccggccga
     4861 agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg gcccgggtct
     4921 gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc gctggctgtc
     4981 gacgctcgag atttccggcg gtggatccgg atcaggctcc ggagacgggt caagcgtctc
     5041 tggatctgga gagggaaggg gaagcctcct aacttgcgga gatgtcgagg agaatcctgg
     5101 cccaatggtt agtaagggag aggagttgat aaaagagaat atgcacatga aactgtatat
     5161 ggaagggaca gtggacaacc atcacttcaa gtgcacctct gagggagaag gaaagccata
     5221 cgaaggcacc cagactatgc gaattaaggt cgtggaggga gggcctctcc ccttcgcctt
     5281 cgatattctt gcaacatctt tcctctacgg gagcaaaacc ttcatcaatc acacacaagg
     5341 catacctgac ttttttaagc agagttttcc agagggcttt acctgggagc gtgtgactac
     5401 atacgaagat gggggtgtgc tgacggctac acaggatact tcacttcagg acggttgtct
     5461 gatctacaac gtcaagatca ggggggtcaa tttcacctcg aatggaccag taatgcaaaa
     5521 aaagaccctg ggatgggaag cttttactga aactctctat cccgccgacg gcggcctcga
     5581 aggcagaaac gatatggctt tgaagctggt tggcgggtcc catctaattg ccaatgccaa
     5641 aaccacatat aggagcaaaa agcctgcaaa gaacctgaag atgccgggtg tgtactatgt
     5701 tgactataga ttagagcgga tcaaagaggc aaataacgaa acgtacgtgg agcagcacga
     5761 ggtagccgtg gcgcgctact gcgatttacc ctccaaactg ggccataaac tgaactgtac
     5821 aggaagcgga gctactaact tcagcctgct gaagcaggct ggcgacgtgg aagaaaaccc
     5881 aggacctatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc
     5941 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag
     6001 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga
     6061 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc
     6121 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct
     6181 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt
     6241 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagaat tcgtcgaggg
     6301 acctaataac ttcgtatagc atacattata cgaagttata catgtttaag ggttccggtt
     6361 ccactaggta caattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     6421 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     6481 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     6541 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     6601 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     6661 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     6721 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     6781 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     6841 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     6901 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     6961 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgatcgagac ctagaaaaac
     7021 atggagcaat cacaagtagc aatacagcag ctaccaatgc tgattgtgcc tggctagaag
     7081 cacaagagga ggaggaggtg ggttttccag tcacacctca ggtaccttta agaccaatga
     7141 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     7201 taattcactc ccaacgaaga caagatatcc ttgatctgtg gatctaccac acacaaggct
     7261 acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca ctgacctttg
     7321 gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc aatgaaggag
     7381 agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg gagagagaag
     7441 tattagagtg gaggtttgac agccgcctag catttcatca catggcccga gagctgcatc
     7501 cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     7561 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     7621 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     7681 aaatctctag cagcatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     7741 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7801 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7861 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7921 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7981 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     8041 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     8101 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     8161 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     8221 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     8281 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     8341 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     8401 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     8461 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     8521 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     8581 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     8641 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     8701 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     8761 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8821 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8881 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8941 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     9001 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     9061 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     9121 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     9181 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     9241 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     9301 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     9361 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     9421 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     9481 catttccccg aaaagtgcca cctgacgctt aagcggtcga cggatcggga gatctcccga
     9541 tcccctatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa gccagtatct
     9601 gctccctgct tgtgtgttgg aggtcgctga gtagtgcgcg agcaaaattt aagctacaac
     9661 aaggcaaggc ttgaccgaca attgcatgaa gaatctgctt agggttaggc gttttgcgct
     9721 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
     9781 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
     9841 tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga cgtcaataat
     9901 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
     9961 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
    10021 tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca tgaccttatg
    10081 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
    10141 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
    10201 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
    10261 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
    10321 ctatataagc agcgcgtttt gcctgtactg ggtctctctg gttagaccag atctgagcct
    10381 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
    10441 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactc
//
