LOCUS       Exported               10696 bp ds-DNA     linear   SYN 11-JUL-2025
DEFINITION  pAT128- lenti 
            pEF-DHFR-MCP-MCP-HALO-RBP-Lib_NANOS1_003-T2A-mtagBFP2-P2A-BSD-WPRE-.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Jul 11, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..10696
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
ORIGIN
        1 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
       61 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      121 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      181 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      241 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      301 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      361 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      421 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      481 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      541 agtaagacca ccgcacagca agcggccggc cgcgctgatc ttcagacctg gaggaggaga
      601 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
      661 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
      721 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
      781 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
      841 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
      901 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
      961 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
     1021 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
     1081 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
     1141 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
     1201 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
     1261 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
     1321 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
     1381 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatcggcact
     1441 gcgtgcgcca attctgcaga caaatggcag tattcatcca caattttaaa agaaaagggg
     1501 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     1561 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     1621 gcagagatcc agtttggtta gtaccgggcc cgctctagag atcccatttg actcaggcgg
     1681 ccgcgtggat aaccgtatta ccgccatgca tgtgcccgtc agtgggcaga gcgcacatcg
     1741 cccacagtcc ccgagaagtt ggggggaggg gtcggcaatt gaaccggtgc ctagagaagg
     1801 tggcgcgggg taaactggga aagtgatgtc gtgtactggc tccgcctttt tcccgagggt
     1861 gggggagaac cgtatataag tgcagtagtc gccgtgaacg ttctttttcg caacgggttt
     1921 gccgccagaa cacaggtaag tgccgtgtgt ggttcccgcg ggcctggcct ctttacgggt
     1981 tatggccctt gcgtgccttg aattacttcc acctggctgc agtacgtgat tcttgatccc
     2041 gagcttcggg ttggaagtgg gtgggagagt tcgaggcctt gcgcttaagg agccccttcg
     2101 cctcgtgctt gagttgaggc ctggcctggg cgctggggcc gccgcgtgcg aatctggtgg
     2161 caccttcgcg cctgtctcgc tgctttcgat aagtctctag ccatttaaaa tttttgatga
     2221 cctgctgcga cgcttttttt ctggcaagat agtcttgtaa atgcgggcca agatctgcac
     2281 actggtattt cggtttttgg ggccgcgggc ggcgacgggg cccgtgcgtc ccagcgcaca
     2341 tgttcggcga ggcggggcct gcgagcgcgg ccaccgagaa tcggacgggg gtagtctcaa
     2401 gctggccggc ctgctctggt gcctggcctc gcgccgccgt gtatcgcccc gccctgggcg
     2461 gcaaggctgg cccggtcggc accagttgcg tgagcggaaa gatggccgct tcccggccct
     2521 gctgcaggga gctcaaaatg gaggacgcgg cgctcgggag agcgggcggg tgagtcaccc
     2581 acacaaagga aaagggcctt tccgtcctca gccgtcgctt catgtgactc cacggagtac
     2641 cgggcgccgt ccaggcacct cgattagttc tcgcgctttt ggagtacgtc gtctttaggt
     2701 tggggggagg ggttttatgc gatggagttt ccccacactg agtgggtgga gactgaagtt
     2761 aggccagctt ggcacttgat gtaattctcc ttggaatttg ccctttttga gtttggatct
     2821 tggttcattc tcaagcctca gacagtggtt caaagttttt ttcttccatt tcaggtgtcg
     2881 tgagctagcc accatgatct cacttattgc tgcactcgca gtcgactacg ttatcgggat
     2941 ggagaacgct atgccatgga atctgcccgc cgacctcgcg tggttcaagc gcaacacatt
     3001 gaacaagccc gttatcatgg gccgacacac ttgggaatcc attggcagac cgctccctgg
     3061 tagaaaaaat atcatactct cctcacagcc ctctactgat gatagagtta catgggtgaa
     3121 atcagtagat gaggcgattg cggcttgtgg cgatgtcccg gaaattatgg ttataggggg
     3181 tgggcgggta attgagcaat ttctccctaa ggcgcagaag ctctacctga cgcacatcga
     3241 cgctgaggta gagggggaca cgcacttccc tgactacgaa cctgacgact gggagtccgt
     3301 gttttctgaa ttccacgacg cggacgcaca gaattcccat tcctactgtt ttgaaatact
     3361 tgaacggcta gccgttaaaa tggcttctaa ctttactcag ttcgttctcg tcgacaatgg
     3421 cggaactggc gacgtgactg tcgccccaag caacttcgct aacgggatcg ctgaatggat
     3481 cagctctaac tcgcgttcac aggcttacaa agtaacctgt agcgttcgtc agagctctgc
     3541 gcagaatcgc aaatacacca tcaaagtcga ggtgcctaaa ggcgcctggc gttcgtactt
     3601 aaatatggaa ctaaccattc caattttcgc cacgaattcc gactgcgagc ttattgttaa
     3661 ggcaatgcaa ggtctcctaa aagatggaaa cccgattccc tcagcaatcg cagcaaactc
     3721 cggcatctac gcgatctacg ccatggcttc tatggcttct aactttactc agttcgttct
     3781 cgtcgacaat ggcggaactg gcgacgtgac tgtcgcccca agcaacttcg ctaacgggat
     3841 cgctgaatgg atcagctcta actcgcgttc acaggcttac aaagtaacct gtagcgttcg
     3901 tcagagctct gcgcagaatc gcaaatacac catcaaagtc gaggtgccta aaggcgcctg
     3961 gcgttcgtac ttaaatatgg aactaaccat tccaattttc gccacgaatt ccgactgcga
     4021 gcttattgtt aaggcaatgc aaggtctcct aaaagatgga aacccgattc cctcagcaat
     4081 cgcagcaaac tccggcatct acgcgatcat ggcagaaatc ggtactggct ttccattcga
     4141 cccccattat gtggaagtcc tgggcgagcg catgcactac gtcgatgttg gtccgcgcga
     4201 tggcacccct gtgctgttcc tgcacggtaa cccgacctcc tcctacgtgt ggcgcaacat
     4261 catcccgcat gttgcaccga cccatcgctg cattgctcca gacctgatcg gtatgggcaa
     4321 atccgacaaa ccagacctgg gttatttctt cgacgaccac gtccgcttca tggatgcctt
     4381 catcgaagcc ctgggtctgg aagaggtcgt cctggtcatt cacgactggg gctccgctct
     4441 gggtttccac tgggccaagc gcaatccaga gcgcgtcaaa ggtattgcat ttatggagtt
     4501 catccgccct atcccgacct gggacgaatg gccagaattt gcccgcgaga ccttccaggc
     4561 cttccgcacc accgacgtcg gccgcaagct gatcatcgat cagaacgttt ttatcgaggg
     4621 tacgctgccg atgggtgtcg tccgcccgct gactgaagtc gagatggacc attaccgcga
     4681 gccgttcctg aatcctgttg accgcgagcc actgtggcgc ttcccaaacg agctgccaat
     4741 cgccggtgag ccagcgaaca tcgtcgcgct ggtcgaagaa tacatggact ggctgcacca
     4801 gtcccctgtc ccgaagctgc tgttctgggg caccccaggc gttctgatcc caccggccga
     4861 agccgctcgc ctggccaaaa gcctgcctaa ctgcaaggct gtggacatcg gcccgggtct
     4921 gaatctgctg caagaagaca acccggacct gatcggcagc gagatcgcgc gctggctgtc
     4981 gacgctcgag atttccggcg gtggatccgg atcaggctcc gctctggtgc ctagtgcaag
     5041 atacgtgtct gcccctggcc ccgctcatcc ccagccattc tccagctgga acgattatct
     5101 cgggctggcg accctgatca caaaggccgt cgacggcgaa ccaaggtttg gctgcgcacg
     5161 gggcggcaat ggaggtgggg gatcaccccc atcctcttcc agcagcagtt gttgctcacc
     5221 tcacactggg gctggtcccg gggccttggg accggccctt ggatctggag agggaagggg
     5281 aagcctccta acttgcggag atgtcgagga gaatcctggc ccaatggtta gtaagggaga
     5341 ggagttgata aaagagaata tgcacatgaa actgtatatg gaagggacag tggacaacca
     5401 tcacttcaag tgcacctctg agggagaagg aaagccatac gaaggcaccc agactatgcg
     5461 aattaaggtc gtggagggag ggcctctccc cttcgccttc gatattcttg caacatcttt
     5521 cctctacggg agcaaaacct tcatcaatca cacacaaggc atacctgact tttttaagca
     5581 gagttttcca gagggcttta cctgggagcg tgtgactaca tacgaagatg ggggtgtgct
     5641 gacggctaca caggatactt cacttcagga cggttgtctg atctacaacg tcaagatcag
     5701 gggggtcaat ttcacctcga atggaccagt aatgcaaaaa aagaccctgg gatgggaagc
     5761 ttttactgaa actctctatc ccgccgacgg cggcctcgaa ggcagaaacg atatggcttt
     5821 gaagctggtt ggcgggtccc atctaattgc caatgccaaa accacatata ggagcaaaaa
     5881 gcctgcaaag aacctgaaga tgccgggtgt gtactatgtt gactatagat tagagcggat
     5941 caaagaggca aataacgaaa cgtacgtgga gcagcacgag gtagccgtgg cgcgctactg
     6001 cgatttaccc tccaaactgg gccataaact gaactgtaca ggaagcggag ctactaactt
     6061 cagcctgctg aagcaggctg gcgacgtgga agaaaaccca ggacctatgg ccaagccttt
     6121 gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca gcatccccat
     6181 ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca tcttcactgg
     6241 tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc tgggcactgc
     6301 tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg agaacagggg
     6361 catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc ctgggatcaa
     6421 agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg aattgctgcc
     6481 ctctggttat gtgtgggagg gctaagaatt cgtcgaggga cctaataact tcgtatagca
     6541 tacattatac gaagttatac atgtttaagg gttccggttc cactaggtac aattcgatat
     6601 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6661 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     6721 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     6781 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     6841 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     6901 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     6961 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     7021 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7081 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7141 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7201 gcatcgatac cgtcgacctc gatcgagacc tagaaaaaca tggagcaatc acaagtagca
     7261 atacagcagc taccaatgct gattgtgcct ggctagaagc acaagaggag gaggaggtgg
     7321 gttttccagt cacacctcag gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7381 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     7441 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tggcagaact
     7501 acacaccagg gccagggatc agatatccac tgacctttgg atggtgctac aagctagtac
     7561 cagttgagca agagaaggta gaagaagcca atgaaggaga gaacacccgc ttgttacacc
     7621 ctgtgagcct gcatgggatg gatgacccgg agagagaagt attagagtgg aggtttgaca
     7681 gccgcctagc atttcatcac atggcccgag agctgcatcc ggactgtact gggtctctct
     7741 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7801 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7861 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatgtgag
     7921 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata
     7981 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc
     8041 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg
     8101 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc
     8161 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg
     8221 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc
     8281 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga
     8341 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg
     8401 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa
     8461 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg
     8521 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt
     8581 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat
     8641 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct
     8701 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta
     8761 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa
     8821 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac
     8881 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa
     8941 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag
     9001 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg
     9061 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag
     9121 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg
     9181 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc
     9241 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat
     9301 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata
     9361 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa
     9421 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca
     9481 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc
     9541 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc
     9601 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg
     9661 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac
     9721 ctgacgctta agcggtcgac ggatcgggag atctcccgat cccctatggt gcactctcag
     9781 tacaatctgc tctgatgccg catagttaag ccagtatctg ctccctgctt gtgtgttgga
     9841 ggtcgctgag tagtgcgcga gcaaaattta agctacaaca aggcaaggct tgaccgacaa
     9901 ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg tacgggccag
     9961 atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta cggggtcatt
    10021 agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg gcccgcctgg
    10081 ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc ccatagtaac
    10141 gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt
    10201 ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca atgacggtaa
    10261 atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta cttggcagta
    10321 catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt acatcaatgg
    10381 gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg acgtcaatgg
    10441 gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca actccgcccc
    10501 attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca gcgcgttttg
    10561 cctgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
    10621 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
    10681 gtctgttgtg tgactc
//
