LOCUS       Exported                6163 bp ds-DNA     circular SYN 10-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 10, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6163
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             2..925
                     /codon_start=1
                     /label=Strep tag-MYCÆCTD,ÆMB0-1-His tag
                     /translation="MWSHPQFEKMPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTAD
                     QLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAAR
                     KDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSA
                     FSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPG
                     KRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVL
                     RQISNNRKCTSPRSSDTEENHHHHHH"
     CDS             928..967
                     /codon_start=1
                     /label=His & S-tags [Split]
                     /note="His "
                     /translation="SSTTTTTTEIRLL"
     misc_feature    961..1089
                     /label=T7 Terminator
     misc_structure  2560..3233
                     /label=pUC origin
                     /note="pUC origin"
     promoter        6077..6094
                     /note="T7 promoter"
     misc_feature    6089
                     /label=T7 transcription initiation site
                     /note="T7 transcription initiation site"
     RBS             6150..6156
                     /note="RBS"
ORIGIN
        1 tatgtggtcc caccctcagt tcgagaagat gccgtcgcgt cgttccggac tttgtagtcc
       61 ttcctatgtc gccgtaaccc cgttttcctt gcgcggtgac aacgacggcg ggggaggtag
      121 cttttctact gcggatcaac ttgaaatggt cacggaactg ctggggggag atatggtaaa
      181 ccagtccttc atctgcgacc ctgatgacga gacatttatt aagaacatta tcatccagga
      241 ttgcatgtgg tcaggctttt cggcagccgc caaactggtc agcgagaaat tggcatctta
      301 tcaggcagca cgtaaggatt ctggaagccc caatcctgcg cgtgggcact cagtctgttc
      361 gacgtccagc ttgtacttgc aagatctgag cgccgctgct agcgaatgta ttgacccaag
      421 cgttgtattc ccttatccac tgaacgatag ttctagcccc aaatcttgcg cctcccaaga
      481 ttcatcagcc ttctcgccga gtagcgatag ccttttaagc tctacagagt cgagtccgca
      541 gggatcaccg gaacctttag tattacatga ggaaaccccg cctactacct catctgattc
      601 ggaggaggag caggaggatg aggaagaaat tgacgtagtc tctgtcgaaa aacgccaagc
      661 tccgggcaag cgcagtgagt cagggagccc ctcggcaggt gggcatagta agccgccaca
      721 tagtccactt gtgctgaagc gctgccacgt ctccacccat cagcataatt atgccgcccc
      781 tcccagcacc cgtaaggatt atccagcagc gaaacgcgtc aaattagact cggtacgcgt
      841 gctgcgccag atcagcaaca atcgcaagtg tacttcgcca cgctcgtcag atacggaaga
      901 gaaccaccac catcatcacc actaacctcg agcaccacca ccaccaccac tgagatccgg
      961 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag caataactag
     1021 cataacccct tggggcctct aaacgggtct tgaggggttt tttgctgaaa ggaggaacta
     1081 tatccggatt ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     1141 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     1201 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     1261 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     1321 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     1381 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     1441 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     1501 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta caatttcagg
     1561 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     1621 aaatatgtat ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact
     1681 gcaatttatt catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg
     1741 aaggagaaaa ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga
     1801 ttccgactcg tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat
     1861 caagtgagaa atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca
     1921 tttctttcca gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat
     1981 caaccaaacc gttattcatt cgtgattgcg cctgagcgag acgaaatacg cgatcgctgt
     2041 taaaaggaca attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat
     2101 caacaatatt ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg
     2161 ggatcgcagt ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg
     2221 gaagaggcat aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg
     2281 caacgctacc tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc
     2341 gatagattgt cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat
     2401 cagcatccat gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc
     2461 tcataacacc ccttgtatta ctgtttatgt aagcagacag ttttattgtt catgaccaaa
     2521 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     2581 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     2641 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     2701 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     2761 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     2821 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     2881 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     2941 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3001 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3061 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3121 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3181 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     3241 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     3301 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     3361 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat atatggtgca
     3421 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatacactc cgctatcgct
     3481 acgtgactgg gtcatggctg cgccccgaca cccgccaaca cccgctgacg cgccctgacg
     3541 ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg ggagctgcat
     3601 gtgtcagagg ttttcaccgt catcaccgaa acgcgcgagg cagctgcggt aaagctcatc
     3661 agcgtggtcg tgaagcgatt cacagatgtc tgcctgttca tccgcgtcca gctcgttgag
     3721 tttctccaga agcgttaatg tctggcttct gataaagcgg gccatgttaa gggcggtttt
     3781 ttcctgtttg gtcactgatg cctccgtgta agggggattt ctgttcatgg gggtaatgat
     3841 accgatgaaa cgagagagga tgctcacgat acgggttact gatgatgaac atgcccggtt
     3901 actggaacgt tgtgagggta aacaactggc ggtatggatg cggcgggacc agagaaaaat
     3961 cactcagggt caatgccagc gcttcgttaa tacagatgta ggtgttccac agggtagcca
     4021 gcagcatcct gcgatgcaga tccggaacat aatggtgcag ggcgctgact tccgcgtttc
     4081 cagactttac gaaacacgga aaccgaagac cattcatgtt gttgctcagg tcgcagacgt
     4141 tttgcagcag cagtcgcttc acgttcgctc gcgtatcggt gattcattct gctaaccagt
     4201 aaggcaaccc cgccagccta gccgggtcct caacgacagg agcacgatca tgcgcacccg
     4261 tggggccgcc atgccggcga taatggcctg cttctcgccg aaacgtttgg tggcgggacc
     4321 agtgacgaag gcttgagcga gggcgtgcaa gattccgaat accgcaagcg acaggccgat
     4381 catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg acccagagcg ctgccggcac
     4441 ctgtcctacg agttgcatga taaagaagac agtcataagt gcggcgacga tagtcatgcc
     4501 ccgcgcccac cggaaggagc tgactgggtt gaaggctctc aagggcatcg gtcgagatcc
     4561 cggtgcctaa tgagtgagct aacttacatt aattgcgttg cgctcactgc ccgctttcca
     4621 gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg
     4681 tttgcgtatt gggcgccagg gtggtttttc ttttcaccag tgagacgggc aacagctgat
     4741 tgcccttcac cgcctggccc tgagagagtt gcagcaagcg gtccacgctg gtttgcccca
     4801 gcaggcgaaa atcctgtttg atggtggtta acggcgggat ataacatgag ctgtcttcgg
     4861 tatcgtcgta tcccactacc gagatgtccg caccaacgcg cagcccggac tcggtaatgg
     4921 cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag catcgcagtg ggaacgatgc
     4981 cctcattcag catttgcatg gtttgttgaa aaccggacat ggcactccag tcgccttccc
     5041 gttccgctat cggctgaatt tgattgcgag tgagatattt atgccagcca gccagacgca
     5101 gacgcgccga gacagaactt aatgggcccg ctaacagcgc gatttgctgg tgacccaatg
     5161 cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg ggagaaaata atactgttga
     5221 tgggtgtctg gtcagagaca tcaagaaata acgccggaac attagtgcag gcagcttcca
     5281 cagcaatggc atcctggtca tccagcggat agttaatgat cagcccactg acgcgttgcg
     5341 cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc gcttcgttct accatcgaca
     5401 ccaccacgct ggcacccagt tgatcggcgc gagatttaat cgccgcgaca atttgcgacg
     5461 gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag caacgactgt ttgcccgcca
     5521 gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc catcgccgct tccacttttt
     5581 cccgcgtttt cgcagaaacg tggctggcct ggttcaccac gcgggaaacg gtctgataag
     5641 agacaccggc atactctgcg acatcgtata acgttactgg tttcacattc accaccctga
     5701 attgactctc ttccgggcgc tatcatgcca taccgcgaaa ggttttgcgc cattcgatgg
     5761 tgtccgggat ctcgacgctc tcccttatgc gactcctgca ttaggaagca gcccagtagt
     5821 aggttgaggc cgttgagcac cgccgccgca aggaatggtg catgcaagga gatggcgccc
     5881 aacagtcccc cggccacggg gcctgccacc atacccacgc cgaaacaagc gctcatgagc
     5941 ccgaagtggc gagcccgatc ttccccatcg gtgatgtcgg cgatataggc gccagcaacc
     6001 gcacctgtgg cgccggtgat gccggccacg atgcgtccgg cgtagaggat cgagatcgat
     6061 ctcgatcccg cgaaattaat acgactcact ataggggaat tgtgagcgga taacaattcc
     6121 cctctagaaa taattttgtt taactttaag aaggagatat aca
//
