LOCUS       Exported                6619 bp ds-DNA     circular SYN 10-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6619)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 10, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..6619
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     CDS             1..40
                     /codon_start=1
                     /label=His & S-tags [Split]
                     /note="His "
                     /translation="SSTTTTTTEIRLL"
     terminator      34..162
                     /note="T7 Terminator"
     misc_structure  1633..2306
                     /label=pUC origin
                     /note="pUC origin"
     promoter        5150..5167
                     /note="T7 promoter"
     misc_feature    5162
                     /label=T7 transcription initiation site
                     /note="T7 transcription initiation site"
     CDS             5215..6618
                     /codon_start=1
                     /label=MYC MutMBII
                     /translation="MAWSHPQFEKGSDYKDDDDKSGMPLNVSFTNRNYDLDYDSVQPYF
                     YCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSL
                     RGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNASAQDCAWSGASAA
                     AKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLN
                     DSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDE
                     EEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKD
                     YPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFAL
                     RDQIPELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRN
                     SCAHHHHHH"
ORIGIN
        1 tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg
       61 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      121 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc
      181 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
      241 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
      301 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
      361 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
      421 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
      481 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
      541 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
      601 ttttaacaaa atattaacgt ttacaatttc aggtggcact tttcggggaa atgtgcgcgg
      661 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgaattaatt
      721 cttagaaaaa ctcatcgagc atcaaatgaa actgcaattt attcatatca ggattatcaa
      781 taccatattt ttgaaaaagc cgtttctgta atgaaggaga aaactcaccg aggcagttcc
      841 ataggatggc aagatcctgg tatcggtctg cgattccgac tcgtccaaca tcaatacaac
      901 ctattaattt cccctcgtca aaaataaggt tatcaagtga gaaatcacca tgagtgacga
      961 ctgaatccgg tgagaatggc aaaagtttat gcatttcttt ccagacttgt tcaacaggcc
     1021 agccattacg ctcgtcatca aaatcactcg catcaaccaa accgttattc attcgtgatt
     1081 gcgcctgagc gagacgaaat acgcgatcgc tgttaaaagg acaattacaa acaggaatcg
     1141 aatgcaaccg gcgcaggaac actgccagcg catcaacaat attttcacct gaatcaggat
     1201 attcttctaa tacctggaat gctgttttcc cggggatcgc agtggtgagt aaccatgcat
     1261 catcaggagt acggataaaa tgcttgatgg tcggaagagg cataaattcc gtcagccagt
     1321 ttagtctgac catctcatct gtaacatcat tggcaacgct acctttgcca tgtttcagaa
     1381 acaactctgg cgcatcgggc ttcccataca atcgatagat tgtcgcacct gattgcccga
     1441 cattatcgcg agcccattta tacccatata aatcagcatc catgttggaa tttaatcgcg
     1501 gcctagagca agacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
     1561 tgtaagcaga cagttttatt gttcatgacc aaaatccctt aacgtgagtt ttcgttccac
     1621 tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc
     1681 gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat
     1741 caagagctac caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat
     1801 actgtccttc tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct
     1861 acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt
     1921 cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg
     1981 gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta
     2041 cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg
     2101 gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg
     2161 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc
     2221 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg
     2281 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat
     2341 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc
     2401 agcgagtcag tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat
     2461 ctgtgcggta tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg
     2521 catagttaag ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg
     2581 acacccgcca acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta
     2641 cagacaagct gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc
     2701 gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat
     2761 gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct
     2821 tctgataaag cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt
     2881 gtaaggggga tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac
     2941 gatacgggtt actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact
     3001 ggcggtatgg atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt
     3061 taatacagat gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa
     3121 cataatggtg cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa
     3181 gaccattcat gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg
     3241 ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt
     3301 cctcaacgac aggagcacga tcatgcgcac ccgtggggcc gccatgccgg cgataatggc
     3361 ctgcttctcg ccgaaacgtt tggtggcggg accagtgacg aaggcttgag cgagggcgtg
     3421 caagattccg aataccgcaa gcgacaggcc gatcatcgtc gcgctccagc gaaagcggtc
     3481 ctcgccgaaa atgacccaga gcgctgccgg cacctgtcct acgagttgca tgataaagaa
     3541 gacagtcata agtgcggcga cgatagtcat gccccgcgcc caccggaagg agctgactgg
     3601 gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga gctaacttac
     3661 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     3721 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc agggtggttt
     3781 ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg ccctgagaga
     3841 gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt ttgatggtgg
     3901 ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact accgagatgt
     3961 ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc gccatctgat
     4021 cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc atggtttgtt
     4081 gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga atttgattgc
     4141 gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa cttaatgggc
     4201 ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg cccagtcgcg
     4261 taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag acatcaagaa
     4321 ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg tcatccagcg
     4381 gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc gccgctttac
     4441 aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc agttgatcgg
     4501 cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga ctggaggtgg
     4561 caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg ttgggaatgt
     4621 aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa acgtggctgg
     4681 cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct gcgacatcgt
     4741 ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg cgctatcatg
     4801 ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg ctctccctta
     4861 tgcgactcct gcattaggaa gcagcccagt agtaggttga ggccgttgag caccgccgcc
     4921 gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc ccccggccac ggggcctgcc
     4981 accataccca cgccgaaaca agcgctcatg agcccgaagt ggcgagcccg atcttcccca
     5041 tcggtgatgt cggcgatata ggcgccagca accgcacctg tggcgccggt gatgccggcc
     5101 acgatgcgtc cggcgtagag gatcgagatc gatctcgatc ccgcgaaatt aatacgactc
     5161 actatagggg aattgtgagc ggataacaat tcccctctag aaaggagata taccatggct
     5221 tggagtcacc cacaatttga aaaaggtagt gattataagg atgacgatga taaatcaggt
     5281 atgccgctca atgtgtcatt tacaaaccgc aactacgact tggactatga ctctgtgcaa
     5341 ccatatttct actgcgatga agaagagaac ttctaccagc agcagcaaca gagtgagctg
     5401 caaccaccag ccccaagcga ggatatttgg aaaaagtttg aactgttgcc gacaccccca
     5461 ctttccccga gtcggcgcag tggattgtgt tcaccttcct acgtagccgt gacgcctttc
     5521 tccctcaggg gagataacga tgggggtgga ggtagttttt caacagcaga tcaattggag
     5581 atggtgaccg aactcctggg tggagatatg gtcaatcagt cattcatttg tgatcccgac
     5641 gatgagacat tcataaaaaa tgcatcagct caggactgcg cttggagcgg agcaagtgca
     5701 gccgctaaat tggtaagcga aaagctcgcg tcttaccaag ccgctcgcaa agatagcggg
     5761 tctcctaatc ctgcgagagg gcattctgtc tgctctactt catccctcta tttgcaggat
     5821 ctttctgctg cggcgagcga atgcatcgat ccttctgtcg tgtttccgta tccccttaat
     5881 gacagctcta gtccgaagtc ttgtgcgagt caagactctt ctgcatttag cccgtctagt
     5941 gactctttgc ttagttctac cgagtcatct ccacaaggtt ctcccgaacc cctcgtgctt
     6001 cacgaggaga ccccgcctac cacgtctagt gatagtgaag aagaacagga ggacgaagag
     6061 gagatcgacg tcgtcagtgt ggagaagcgc caagcaccgg ggaagagatc cgagagcggg
     6121 tctcccagtg cggggggtca cagtaaaccc cctcattctc cgcttgtcct taaacgatgt
     6181 cacgtgtcta ctcaccagca caattacgct gcgccgcctt ctactcgaaa ggactatcct
     6241 gcagccaaga gggtcaagct cgatagtgtg agggtcttga gacagatttc taacaatcgg
     6301 aaatgcacat ccccccggtc cagtgacacg gaggaaaatg tcaagagacg cacacacaat
     6361 gtactggaaa ggcagaggag gaacgagctg aagagatcat tctttgcctt gcgagaccaa
     6421 atacctgaac tggagaataa tgagaaagcc ccgaaggtag taattttgaa aaaggccacg
     6481 gcctacatac tgtctgtaca agcagaagag cagaaactga ttagtgaaga agacctgttg
     6541 cgaaagcggc gagagcaact caaacataag ttggaacaac tgcgcaattc ttgcgctcac
     6601 catcatcatc atcattagc
//
