LOCUS       Exported               10379 bp ds-DNA     circular SYN 10-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10379)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 10, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1..866,1378..10379)
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          996..1001
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label= [Split]
                     /note=" [Split]"
     source          1262..1377
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label= [Split]
                     /note=" [Split]"
     stem_loop       1..1126
                     /note="8851 to 9101 of Multi-mini-sh:Multi-sh1 PCR 1937 
                     plus 252 to 135 of pCF806 shMYC2005:2046"
                     /note="/date=21-Mar-2024"
                     /note="/left_cutter=BbsI (4432)"
                     /note="/left_end=5AATT"
                     /note="/left_join=keep end"
                     /note="/right_cutter=EcoRI (135)"
                     /note="/right_end=5AATT"
                     /note="/right_join=keep end"
     stem_loop       1..1126
                     /label=252 to 135 of pCF806 shMYC2005:2046
                     /note="252 to 135 of pCF806 shMYC2005:2046"
                     /note="/date=21-Mar-2024"
                     /note="/left_cutter=MluI (251)"
                     /note="/left_end=5CGCG"
                     /note="/right_cutter=EcoRI (135)"
                     /note="/right_end=5AATT"
     stem_loop       1..991
     stem_loop       1..991
                     /label=4660 to 4543 of pCF806 shMYC2005
                     /note="4660 to 4543 of pCF806 shMYC2005"
                     /note="/date=28-Feb-2024"
                     /note="/left_cutter=MluI (4659)"
                     /note="/left_end=5CGCG"
                     /note="/right_cutter=EcoRI (4543)"
                     /note="/right_end=5AATT"
     stem_loop       1..991
                     /note="4431 to 4418 of pCF806 Vector plus 6 to 130 of 
                     shMYC2005 PCR"
                     /note="/date=20-Jul-2023"
                     /note="/left_cutter=EcoRI (4430)"
                     /note="/left_end=5AATT"
                     /note="/left_join=keep end"
                     /note="/right_cutter=EcoRI (130)"
                     /note="/right_end=5AATT"
                     /note="/right_join=keep end"
     stem_loop       1..866
                     /label=4431 to 4418 of pCF806 Vector
                     /note="4431 to 4418 of pCF806 Vector"
                     /note="/date=19-Jul-2023"
                     /note="/left_cutter=EcoRI (4430)"
                     /note="/left_end=5AATT"
                     /note="/right_cutter=XhoI (4418)"
                     /note="/right_end=5TCGA"
     CDS             10..729
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(55..76)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(316..335)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     663..684
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     stem_loop       900..960
                     /label=MYC miR 2005
     stem_loop       1002..1126
     stem_loop       1035..1095
                     /label=MYC miR 2046
     stem_loop       1170..1230
                     /label=MYC miR 1937
     stem_loop       1262..1377
     stem_loop       1378..10379
     misc_feature    1439..1650
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor  
                     EF-1-alpha"
     CDS             1675..2274
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1675..1694)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also  
                     called puro-variant-R"
     primer_bind     2171..2191
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2297..2882
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the  
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2464..2481)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer.  
                     Also called pCDH-rev"
     primer_bind     2691..2710
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2883..3629
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to  
                     promoters containing the tet operator in the presence of  
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3649..4237
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional  
                     regulatory element"
     primer_bind     complement(3702..3722)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4120..4131)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4309..4542
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4568..4585)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer.  
                     Also called BGH reverse"
     misc_feature    4574..4798
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4904..4921)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      5075..5663
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
     primer_bind     complement(5155..5174)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5834..6694)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6457..6476
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_feature    complement(6695..6799)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6874..6893)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable  
                     marker"
     misc_feature    7059..7438
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    7440..7638
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
     primer_bind     7593..7613
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7656..7836
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7883..8008
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus  
                     type 1"
     misc_feature    8501..8734
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for  
                     Rev-dependent mRNA export from the nucleus to the  
                     cytoplasm."
     CDS             8919..8963
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids  
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik  
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9261..9378
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination  
                     sequence of HIV-1"
     misc_feature    10030..10379
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be  
                     activated by binding of Tet-On(R) 3G, optimized for  
                     retroviral and lentiviral vectors"
     protein_bind    10038..10056
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10074..10092
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10110..10128
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10146..10164
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10182..10200
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10218..10236
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10254..10272
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10318..10342
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggatccacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc
       61 gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ctggcgaggg cgagggcgat
      121 gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc
      181 tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac
      241 cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc
      301 accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc
      361 gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc
      421 ctggggcaca agctggagta caactacaac agccacaacg tctatatcat ggccgacaag
      481 cagaagaacg gcatcaaggc gaacttcaag atccgccaca acatcgagga cggcagcgtg
      541 cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc
      601 gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat
      661 cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg
      721 tacaagtaat gacgtacggc ggccgcctcg actagggata acagggtaat tgtttgaatg
      781 aggcttcagt actttacaga atcgttgcct gcacatcttg gaaacacttg ctgggattac
      841 ttcgacttct taacccaaca gaaggctcga gaaggtatat tgctgttgac agtgagcgaa
      901 aagatttagc cataatgtaa tagtgaagcc acagatgtat tacattatgg ctaaatcttt
      961 ctgcctactg cctcggactt caaggggcta gaattgaagg ctcgagaagg tatattgctg
     1021 ttgacagtga gcgcgggcat aaaagaactt ttttatagtg aagccacaga tgtataaaaa
     1081 agttctttta tgcccatgcc tactgcctcg gacttcaagg ggctagaatt gaaggctcga
     1141 gaaggtatat tgctgttgac agtgagcgcc acctatgaac ttgtttcaaa tagtgaagcc
     1201 acagatgtat ttgaaacaag ttcataggtg atgcctactg cctcggactt caaggggcta
     1261 gaattcgagc aattatcttg tttactaaaa ctgaatacct tgctatctct ttgatacatt
     1321 tttacaaagc tgaattaaaa tggtataaat taaatcactt ttttcaattg acacgtacgc
     1381 gtgatctaat tctactaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     1441 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgatcc
     1501 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     1561 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     1621 tttcgcaacg ggtttgccgc cagaacacag gaccggttgt agagcgctgc caccatgacc
     1681 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     1741 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     1801 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     1861 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     1921 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     1981 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     2041 tggttcctgg ccaccgtcgg agtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     2101 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     2161 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     2221 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgaacgccg
     2281 ccatcgataa ggagcgcccc tctccctccc ccccccctaa cgttactggc cgaagccgct
     2341 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
     2401 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
     2461 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
     2521 aagcttcttg aagacaaaca acgtctgtag cgaccctttg caggcagcgg aaccccccac
     2581 ctggcgacag gtgcctctgc ggccaaaagc cacgtgtata agatacacct gcaaaggcgg
     2641 cacaacccca gtgccacgtt gtgagttgga tagttgtgga aagagtcaaa tggctctcct
     2701 caagcgtatt caacaagggg ctgaaggatg cccagaaggt accccattgt atgggatctg
     2761 atctggggcc tcggtgcaca tgctttacat gtgtttagtc gaggttaaaa aacgtctagg
     2821 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacaa
     2881 ccatgagcag actggacaag agcaaagtca taaactctgc tctggaatta ctcaatggag
     2941 tcggtatcga aggcctgacg acaaggaaac tcgctcaaaa gctgggagtt gagcagccta
     3001 ccctgtactg gcacgtgaag aacaagcggg ccctgctcga tgccctgcca atcgagatgc
     3061 tggacaggca tcatacccac tcctgccccc tggaaggcga gtcatggcaa gactttctgc
     3121 ggaacaacgc caagtcatac cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc
     3181 atctcggcac ccgcccaaca gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc
     3241 tgtgtcagca aggcttctcc ctggagaacg cactgtacgc tctgtccgcc gtgggccact
     3301 ttacactggg ctgcgtattg gaggaacagg agcatcaagt agcaaaagag gaaagagaga
     3361 cacctaccac cgattctatg cccccacttc tgaaacaagc aattgagctg ttcgaccggc
     3421 agggagccga acctgccttc cttttcggcc tggaactaat catatgtggc ctggagaaac
     3481 agctaaagtg cgaaagcggc gggccgaccg acgcccttga cgattttgac ttagacatgc
     3541 tcccagccga tgcccttgac gactttgacc ttgatatgct gcctgctgac gctcttgacg
     3601 attttgacct tgacatgctc cccgggtaag ctctagagtt aagtcgacaa tcaacctctg
     3661 gattacaaaa tttgtgaaag attgactggt attcttaact atgttgctcc ttttacgcta
     3721 tgtggatacg ctgctttaat gcctttgtat catgctattg cttcccgtat ggctttcatt
     3781 ttctcctcct tgtataaatc ctggttgctg tctctttatg aggagttgtg gcccgttgtc
     3841 aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa cccccactgg ttggggcatt
     3901 gccaccacct gtcagctcct ttccgggact ttcgctttcc ccctccctat tgccacggcg
     3961 gaactcatcg ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac
     4021 aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc
     4081 acctggattc tgcgcgggac gtccttctgc tacgtccctt cggccctcaa tccagcggac
     4141 cttccttccc gcggcctgct gccggctctg cggcctcttc cgcgtcttcg ccttcgccct
     4201 cagacgagtc ggatctccct ttgggccgcc tccccgcgtc gactttaaga ccaatgactt
     4261 acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg gaagggctaa
     4321 ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc tggttagacc
     4381 agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa
     4441 gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga
     4501 gatccctcag acccttttag tcagtgtgga aaatctctag cagggcccgt ttaaacccgc
     4561 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
     4621 ccttccttga ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt
     4681 gcatcgcatt gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc
     4741 aagggggagg attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct
     4801 tctgaggcgg aaagaaccag ccattaatga aaagaaccag ccattaatga atcggccaac
     4861 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
     4921 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
     4981 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
     5041 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
     5101 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
     5161 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
     5221 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
     5281 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
     5341 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa
     5401 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg
     5461 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag
     5521 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt
     5581 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta
     5641 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     5701 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     5761 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     5821 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     5881 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     5941 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     6001 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     6061 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     6121 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     6181 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     6241 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     6301 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     6361 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     6421 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     6481 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     6541 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     6601 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     6661 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     6721 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     6781 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc gacggatcgg
     6841 gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     6901 aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat
     6961 ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc ttagggttag
     7021 gcgttttgcg ctgcttcgcg atgtacgggc cagatattga cattgattat tgactagtta
     7081 ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac
     7141 ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc
     7201 aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt
     7261 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
     7321 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac
     7381 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
     7441 gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac ggggatttcc
     7501 aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt
     7561 tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg
     7621 ggaggtctat ataagcagcg cgttttgcct gtactgggtc tctctggtta gaccagatct
     7681 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     7741 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     7801 tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa
     7861 agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac
     7921 ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta
     7981 gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg
     8041 ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg
     8101 ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg
     8161 ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag aagaacttag
     8221 atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga
     8281 caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca
     8341 gcaagcggcc gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg
     8401 aattatataa atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa
     8461 agagaagagt ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt
     8521 tcttgggagc agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca
     8581 gacaattatt gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc
     8641 aacagcatct gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg
     8701 ctgtggaaag atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac
     8761 tcatttgcac cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga
     8821 tttggaatca cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa
     8881 tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg
     8941 aattagataa atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata
     9001 taaaattatt cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac
     9061 tttctatagt gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc
     9121 caaccccgag gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca
     9181 gagacagatc cattcgatta gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa
     9241 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     9301 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     9361 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggttaatt
     9421 aaggtaccgg gaggtggtcc ctgcagttac gccaatgata acccccgcca gaaaaatctt
     9481 agtagccttc cctttttgtt ttccgtgccc caactcggcg gattgactcg gccccttccg
     9541 gaaacacccg aatcaacttc tagtcaaatt attgttcacg ccgcaatgac ccacccctgg
     9601 cccgcgtctg tggaactgac ccctggtgta caggagagtt cgctgctgaa agtggtccca
     9661 aaggggtact agtttttaag ctcccaactc cccctccccc agcgtctgga ggattccaca
     9721 ccctcgcacc gcaggggcga ggaagtgggc ggagtccggt tttggcgcca gccgctgagg
     9781 ctgccaagca gaaaagccac cgctgaggag actccggtca ctgtcctcgc cccgcctccc
     9841 ccttccctcc ccttggggac caccgggcgc cacgccgcga acggtaagtg ccgcggtcgt
     9901 cggcgcctcc gccctccccc tagggcccca attcccagcg ggcgcggcgc gcggccccgc
     9961 gcgcagctcc cggctccctc ccccttcgga tgtggcttga gctgtaggcg cggaggctag
    10021 cctagattcg agtttactcc ctatcagtga tagagaacgt atgaagagtt tactccctat
    10081 cagtgataga gaacgtatgc agactttact ccctatcagt gatagagaac gtataaggag
    10141 tttactccct atcagtgata gagaacgtat gaccagttta ctccctatca gtgatagaga
    10201 acgtatctac agtttactcc ctatcagtga tagagaacgt atatccagtt tactccctat
    10261 cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg ggcgcctata aaagcagagc
    10321 tcgtttagtg aaccgtcaga tcgcctggag caattccaca acacttttgt cttatactt
//
