LOCUS       Exported               11030 bp ds-DNA     circular SYN 10-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11030)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 10, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11030
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1
     source          1
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1647..1652
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label= [Split]
                     /note=" [Split]"
     source          1913..2028
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label= [Split]
                     /note=" [Split]"
     source          2029..11030
     source          2029..11030
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     stem_loop       1..1777
                     /note="8851 to 9101 of Multi-mini-sh:Multi-sh1 PCR 1937 
                     plus 252 to 135 of of pCF806 M67 shMYC2005:2046"
                     /note="/date=21-Mar-2024"
                     /note="/left_cutter=BbsI (4432)"
                     /note="/left_end=5AATT"
                     /note="/left_join=keep end"
                     /note="/right_cutter=EcoRI (135)"
                     /note="/right_end=5AATT"
                     /note="/right_join=keep end"
     stem_loop       1..1777
                     /label=252 to 135 of of pCF806 M67 shMYC2005:2046
                     /note="252 to 135 of of pCF806 M67 shMYC2005:2046"
                     /note="/date=21-Mar-2024"
                     /note="/left_cutter=MluI (251)"
                     /note="/left_end=5CGCG"
                     /note="/right_cutter=EcoRI (135)"
                     /note="/right_end=5AATT"
     stem_loop       1..1642
     stem_loop       1..1642
                     /label=5311 to 5194 of pCF806 M67 shMYC2005
                     /note="5311 to 5194 of pCF806 M67 shMYC2005"
                     /note="/date=21-Mar-2024"
                     /note="/left_cutter=MluI (5310)"
                     /note="/left_end=5CGCG"
                     /note="/right_cutter=EcoRI (5194)"
                     /note="/right_end=5AATT"
     CDS             1
                     /codon_start=1
                     /translation=""
     CDS             1
                     /codon_start=1
                     /translation=""
     stem_loop       1
                     /label=4431 to 4418 of pCF806 Vector
                     /note="4431 to 4418 of pCF806 Vector"
                     /note="/date=19-Jul-2023"
                     /note="/left_cutter=EcoRI (4430)"
                     /note="/left_end=5AATT"
                     /note="/right_cutter=XhoI (4418)"
                     /note="/right_end=5TCGA"
     CDS             10..1380
                     /codon_start=1
                     /label=V5-MYC Mut MBIV
                     /translation="MGKPIPNPLLGLDSTGSMPLNVSFTNRNYDLDYDSVQPYFYCDEE
                     ENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDND
                     GGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVS
                     EKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSP
                     KSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDV
                     VSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYEDGGSTAASYPAAK
                     RVKLDSVRVLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIP
                     ELENNEKAPKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     stem_loop       1551..1611
                     /label=MYC miR 2005
     stem_loop       1653..1777
     stem_loop       1686..1746
                     /label=MYC miR 2046
     stem_loop       1821..1881
                     /label=MYC miR 1937
     stem_loop       1913..2028
     CDS             2029..11030
                     /codon_start=1
                     /translation="RVI*FY*VLKGVGIGSGARQWAERTSPTVPEKLGGGVGN*SGA*R
                     RWRGVNWESDVVYWLRLFPEGGGEPYISAVVAVNVLFRNGFAARTQDRL*SAATMTEYK
                     PTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKV
                     WVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAW
                     FLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADV
                     EVPEGPRTWCMTRKPGA*TPPSIRSAPLPPPPLTLLAEAAWNKAGVRLSICYFPPYCRL
                     LAM*GPGNLALSS*RAFLGVFPLSPKECKVC*MS*RKQFLWKLLEDKQRL*RPFAGSGT
                     PHLATGASAAKSHVYKIHLQRRHNPSATL*VG*LWKESNGSPQAYSTRG*RMPRRYPIV
                     WDLIWGLGAHALHVFSRG*KTSRPPEPRGRGFPLKNTMIIWPQP*ADWTRAKS*TLLWN
                     YSMESVSKA*RQGNSLKSWELSSLPCTGT*RTSGPCSMPCQSRCWTGIIPTPAPWKASH
                     GKTFCGTTPSHTAVLSSHIATGLKCISAPAQQRNSTKPWKISSRSCVSKASPWRTHCTL
                     CPPWATLHWAAYWRNRSIK*QKRKERHLPPILCPHF*NKQLSCSTGREPNLPSFSAWN*
                     SYVAWRNS*SAKAAGRPTPLTILT*TCSQPMPLTTLTLICCLLTLLTILTLTCSPGKL*
                     S*VDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRMAFIFSSLYKSWLLS
                     LYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARC
                     WTGARLLGTDNSVVLSGKSSSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPA
                     LRPLPRLRLRPQTSRISLWAASPRRL*DQ*LTRQL*ILATF*KKRGDWKG*FTPNEDKI
                     CFLLVLGLSG*TRSEPGSSLAN*GTHCLSLNKACLECFK*CVPVCCVTLVTRDPSDPFS
                     QCGKSLAGPV*TR*SASTVPSSCQPSVVCPSPVPSLTLEGATPTVLS**NEEIASHCLS
                     RCHSILGGGVGQDSKGEDWEDNSRHAGDAVGSMASEAERTSH**KEPAINESANARGEA
                     VCVLGALPLPRSLTRCARSFGCGERYQLTQRR*YGYPQNQGITQERTCEQKASKRPGTV
                     KRPRCWRFSIGSAPLTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDT
                     CPPFSLREAWRFLIAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*DT
                     TYRHWQQPLVTGLAERGM*AVLQSS*SGGLTTATLEEQYLVSALC*SQLPSEKELVALD
                     PANKPPLVAVVFLFASSRLRAEKKDLKKIL*SFLRGLTLSGTKTHVKGFWS*DYQKGSS
                     PRSF*IKNEVLNQSKVYMSKLGLTVTNA*SVRHLSQRSVYFVHP*LPDSPSCR*LRYGR
                     AYHLAPVLQ*YRETHAHRLQIYQQ*TSQPEGPSAEVVLQLYPPPSSLLIVAGKLE*VVR
                     QLIVCATLLPLLQASWCHARRLVWLHSAPVPNDQGELHDPPCCAKKRLAPSVLRSLSEV
                     SWPQCYHSWLWQHCIILLLSCHP*DAFL*LVSTQPSHSENSVCGDRVALARRQYGIIPR
                     HIAEL*KCSSLENVLRGENSQGSYRC*DPVRCNPLVHPTDLQHLLLSPAFLGEQKQEGK
                     MPQKRE*GRHGNVEYSYSSFFNIIEAFIRVIVS*ADTYLNVFRKINK*GFRAHFPEKCH
                     LTSTDREISRSPMVHSQYNLL*CRIVKPVSAPCLCVGGR*VVREQNLSYNKARLDRQLH
                     EESA*G*AFCAASRCTGQILTLIID*LLIVINYGVISS*PIYGVPRYITYGKWPAWLTA
                     QRPPPIDVNNDVCSHSNANRDFPLTSMGGVFTVNCPLGSTSSVSYAKYAPY*RQ*R*MA
                     RLALCPVHDLMGLSYLAVHLRISHRYYHGDAVLAVHQWAWIAV*LTGISKSPPH*RQWE
                     FVLAPKSTGLSKMS*QLRPIDANGR*ACTVGGLYKQRVLPVLGLSG*TRSEPGSSLAN*
                     GTHCLSLNKACLECFK*CVPVCCVTLVTRDPSDPFSQCGKSLAVAPEQGLESERETRGA
                     LSTQDSAC*SAHGKRRGAATGEYAKNFD*RRLEGERWVRERQY*AGEN*IAMGKNSVKA
                     RGKEKI*IKTYSMGKQGARTIRS*SWPVRNIRRL*TNTGTATTIPSDRIRRT*III*YS
                     SNPLLCASKDRDKRHQGSFRQDRGRAKQK*DHRTASGR*SSDLEEEI*GTIGEVNYINI
                     K**KLNH*E*HPPRQREEWCREKKEQWE*ELCSLGSWEQQEALWAQRQ*R*RYRPDNYC
                     LV*CSSRTIC*GLLRRNSICCNSQSGASSSSRQESWLWKDT*RINSSWGFGVALENSFA
                     PLLCLGMLVGVINLWNRFGITRPGWSGTEKLTITQA*YTP*LKNRKTSKKRMNKNYWN*
                     INGQVCGIGLT*QIGCGI*NYS****EAW*V*E*FLLYFL**IELGRDIHHYRFRPTSQ
                     PRGDPTGPKE*KKKVERETETDPFD**TDRHCVRQFCRQMAVFIHNFKRKGGIGGYSAG
                     ERIVDIIATDIQTKELQKQITKIQNFRVYYRDSRDPVWLIKVPGGGPCSYANDNPRQKN
                     LSSLPFLFSVPQLGGLTRPLPETPESTSSQIIVHAAMTHPWPASVELTPGVQESSLLKV
                     VPKGY*FLSSQLPLPQRLEDSTPSHRRGEEVGGVRFWRQPLRLPSRKATAEETPVTVLA
                     PPPPSLPLGTTGRHAANGKCRGRRRLRPPPRAPIPSGRGARPRAQLPAPSPFGCGLSCR
                     RGG*PRFEFTPYQ**RTYEEFTPYQ**RTYADFTPYQ**RTYKEFTPYQ**RTYDQFTP
                     YQ**RTYLQFTPYQ**RTYIQFTPYQ**RTYVEVGVYGGRL*KQSSFSEPSDRLEQFHN
                     TFVLY"
     CDS             2029..11030
                     /codon_start=1
                     /translation="RVI*FY*VLKGVGIGSGARQWAERTSPTVPEKLGGGVGN*SGA*R
                     RWRGVNWESDVVYWLRLFPEGGGEPYISAVVAVNVLFRNGFAARTQDRL*SAATMTEYK
                     PTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERVTELQELFLTRVGLDIGKV
                     WVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAAQQQMEGLLAPHRPKEPAW
                     FLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSAPRNLPFYERLGFTVTADV
                     EVPEGPRTWCMTRKPGA*TPPSIRSAPLPPPPLTLLAEAAWNKAGVRLSICYFPPYCRL
                     LAM*GPGNLALSS*RAFLGVFPLSPKECKVC*MS*RKQFLWKLLEDKQRL*RPFAGSGT
                     PHLATGASAAKSHVYKIHLQRRHNPSATL*VG*LWKESNGSPQAYSTRG*RMPRRYPIV
                     WDLIWGLGAHALHVFSRG*KTSRPPEPRGRGFPLKNTMIIWPQP*ADWTRAKS*TLLWN
                     YSMESVSKA*RQGNSLKSWELSSLPCTGT*RTSGPCSMPCQSRCWTGIIPTPAPWKASH
                     GKTFCGTTPSHTAVLSSHIATGLKCISAPAQQRNSTKPWKISSRSCVSKASPWRTHCTL
                     CPPWATLHWAAYWRNRSIK*QKRKERHLPPILCPHF*NKQLSCSTGREPNLPSFSAWN*
                     SYVAWRNS*SAKAAGRPTPLTILT*TCSQPMPLTTLTLICCLLTLLTILTLTCSPGKL*
                     S*VDNQPLDYKICERLTGILNYVAPFTLCGYAALMPLYHAIASRMAFIFSSLYKSWLLS
                     LYEELWPVVRQRGVVCTVFADATPTGWGIATTCQLLSGTFAFPLPIATAELIAACLARC
                     WTGARLLGTDNSVVLSGKSSSFPWLLACVATWILRGTSFCYVPSALNPADLPSRGLLPA
                     LRPLPRLRLRPQTSRISLWAASPRRL*DQ*LTRQL*ILATF*KKRGDWKG*FTPNEDKI
                     CFLLVLGLSG*TRSEPGSSLAN*GTHCLSLNKACLECFK*CVPVCCVTLVTRDPSDPFS
                     QCGKSLAGPV*TR*SASTVPSSCQPSVVCPSPVPSLTLEGATPTVLS**NEEIASHCLS
                     RCHSILGGGVGQDSKGEDWEDNSRHAGDAVGSMASEAERTSH**KEPAINESANARGEA
                     VCVLGALPLPRSLTRCARSFGCGERYQLTQRR*YGYPQNQGITQERTCEQKASKRPGTV
                     KRPRCWRFSIGSAPLTSITKIDAQVRGGETRQDYKDTRRFPLEAPSCALLFRPCRLPDT
                     CPPFSLREAWRFLIAHAVGISVRCRSFAPSWAVCTNPPFSPTAAPYPVTIVLSPTR*DT
                     TYRHWQQPLVTGLAERGM*AVLQSS*SGGLTTATLEEQYLVSALC*SQLPSEKELVALD
                     PANKPPLVAVVFLFASSRLRAEKKDLKKIL*SFLRGLTLSGTKTHVKGFWS*DYQKGSS
                     PRSF*IKNEVLNQSKVYMSKLGLTVTNA*SVRHLSQRSVYFVHP*LPDSPSCR*LRYGR
                     AYHLAPVLQ*YRETHAHRLQIYQQ*TSQPEGPSAEVVLQLYPPPSSLLIVAGKLE*VVR
                     QLIVCATLLPLLQASWCHARRLVWLHSAPVPNDQGELHDPPCCAKKRLAPSVLRSLSEV
                     SWPQCYHSWLWQHCIILLLSCHP*DAFL*LVSTQPSHSENSVCGDRVALARRQYGIIPR
                     HIAEL*KCSSLENVLRGENSQGSYRC*DPVRCNPLVHPTDLQHLLLSPAFLGEQKQEGK
                     MPQKRE*GRHGNVEYSYSSFFNIIEAFIRVIVS*ADTYLNVFRKINK*GFRAHFPEKCH
                     LTSTDREISRSPMVHSQYNLL*CRIVKPVSAPCLCVGGR*VVREQNLSYNKARLDRQLH
                     EESA*G*AFCAASRCTGQILTLIID*LLIVINYGVISS*PIYGVPRYITYGKWPAWLTA
                     QRPPPIDVNNDVCSHSNANRDFPLTSMGGVFTVNCPLGSTSSVSYAKYAPY*RQ*R*MA
                     RLALCPVHDLMGLSYLAVHLRISHRYYHGDAVLAVHQWAWIAV*LTGISKSPPH*RQWE
                     FVLAPKSTGLSKMS*QLRPIDANGR*ACTVGGLYKQRVLPVLGLSG*TRSEPGSSLAN*
                     GTHCLSLNKACLECFK*CVPVCCVTLVTRDPSDPFSQCGKSLAVAPEQGLESERETRGA
                     LSTQDSAC*SAHGKRRGAATGEYAKNFD*RRLEGERWVRERQY*AGEN*IAMGKNSVKA
                     RGKEKI*IKTYSMGKQGARTIRS*SWPVRNIRRL*TNTGTATTIPSDRIRRT*III*YS
                     SNPLLCASKDRDKRHQGSFRQDRGRAKQK*DHRTASGR*SSDLEEEI*GTIGEVNYINI
                     K**KLNH*E*HPPRQREEWCREKKEQWE*ELCSLGSWEQQEALWAQRQ*R*RYRPDNYC
                     LV*CSSRTIC*GLLRRNSICCNSQSGASSSSRQESWLWKDT*RINSSWGFGVALENSFA
                     PLLCLGMLVGVINLWNRFGITRPGWSGTEKLTITQA*YTP*LKNRKTSKKRMNKNYWN*
                     INGQVCGIGLT*QIGCGI*NYS****EAW*V*E*FLLYFL**IELGRDIHHYRFRPTSQ
                     PRGDPTGPKE*KKKVERETETDPFD**TDRHCVRQFCRQMAVFIHNFKRKGGIGGYSAG
                     ERIVDIIATDIQTKELQKQITKIQNFRVYYRDSRDPVWLIKVPGGGPCSYANDNPRQKN
                     LSSLPFLFSVPQLGGLTRPLPETPESTSSQIIVHAAMTHPWPASVELTPGVQESSLLKV
                     VPKGY*FLSSQLPLPQRLEDSTPSHRRGEEVGGVRFWRQPLRLPSRKATAEETPVTVLA
                     PPPPSLPLGTTGRHAANGKCRGRRRLRPPPRAPIPSGRGARPRAQLPAPSPFGCGLSCR
                     RGG*PRFEFTPYQ**RTYEEFTPYQ**RTYADFTPYQ**RTYKEFTPYQ**RTYDQFTP
                     YQ**RTYLQFTPYQ**RTYIQFTPYQ**RTYVEVGVYGGRL*KQSSFSEPSDRLEQFHN
                     TFVLY"
     stem_loop       2029..11030
     misc_feature    2090..2301
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor  
                     EF-1-alpha"
     CDS             2326..2925
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2326..2345)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also  
                     called puro-variant-R"
     primer_bind     2822..2842
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2948..3533
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the  
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3115..3132)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer.  
                     Also called pCDH-rev"
     primer_bind     3342..3361
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3534..4280
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to  
                     promoters containing the tet operator in the presence of  
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4300..4888
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional  
                     regulatory element"
     primer_bind     complement(4353..4373)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4771..4782)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4960..5193
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5219..5236)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer.  
                     Also called BGH reverse"
     misc_feature    5225..5449
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5555..5572)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_structure  5667..6340
                     /label=pUC origin
                     /note="pUC origin"
     rep_origin      5726..6314
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
     primer_bind     complement(5806..5825)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     gene            6485..7345
                     /label=Ampicillin Resistance
                     /note="Ampicillin Resistance"
     CDS             complement(6485..7345)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7108..7127
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_feature    complement(7346..7450)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7525..7544)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable  
                     marker"
     misc_feature    7710..8089
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    8091..8289
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
     primer_bind     8244..8264
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8307..8487
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8534..8659
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus  
                     type 1"
     misc_feature    9152..9385
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for  
                     Rev-dependent mRNA export from the nucleus to the  
                     cytoplasm."
     CDS             9570..9614
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids  
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik  
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9912..10029
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination  
                     sequence of HIV-1"
     misc_feature    10681..11030
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be  
                     activated by binding of Tet-On(R) 3G, optimized for  
                     retroviral and lentiviral vectors"
     protein_bind    10689..10707
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10725..10743
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10761..10779
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10797..10815
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10833..10851
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10869..10887
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10905..10923
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10969..10993
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggatccacca tgggaaaacc tatcccaaat cccctgttgg gcctggactc tacaggctca
       61 atgcccttga atgtgagctt cacaaacagg aattacgatc tcgattatga ctccgtacag
      121 ccctactttt attgtgacga agaagagaat ttctatcagc agcagcagca gagcgaactc
      181 cagccccccg ctccttcaga agacatctgg aaaaaattcg aactcctgcc taccccaccc
      241 ctgtccccat cacgccgctc cgggttgtgc tccccatctt acgtggctgt tactccgttc
      301 agcttaagag gcgataatga tggcgggggg gggagtttta gtaccgccga ccagttggaa
      361 atggtcacag aactgctggg cggggacatg gtgaaccagt cattcatctg cgacccagac
      421 gacgagactt tcattaagaa tattattatt caagattgca tgtggagtgg attcagtgcc
      481 gcggccaaac ttgtgagcga gaagctggcc tcttaccaag ccgccagaaa ggacagcggg
      541 tctcctaacc cagcacgagg acacagcgtt tgtagcacct cctctctcta tctgcaggat
      601 ctctctgcag cagcctccga atgtattgat ccttccgtcg tctttcccta cccacttaac
      661 gacagttcct cccctaagag ctgtgccagt caggatagct ccgcatttag ccctagtagc
      721 gatagccttc tgtcctcaac tgagtcttca cctcagggta gtcccgaacc tttggtactg
      781 cacgaagaga caccccccac gactagtagt gattctgagg aggagcagga ggacgaggaa
      841 gaaatagatg tagtgtccgt ggagaagcgc caggcccctg gcaagcggag tgagagtggc
      901 agtccgtcag ccggcggcca ttccaaaccg ccccacagcc ccttggtact gaagcgctgt
      961 cacgtgagta ctcaccagca caactacgaa gatggcggca gtacagccgc gagctaccct
     1021 gcagccaaga gagtgaagct tgacagtgta agggtattga gacagatttc caataacagg
     1081 aagtgcacta gccccaggtc ctcagacacc gaggagaacg tcaaacgacg aacacacaac
     1141 gtcctggaac ggcagaggcg gaacgaactt aagagatcct tcttcgcact gagggatcag
     1201 attccagaac tggaaaacaa tgagaaagct cccaaggttg tgatactcaa gaaagccacg
     1261 gcatatatcc tgagcgttca ggcagaggag caaaaactga tcagcgagga ggacctcttg
     1321 cgcaagagac gcgagcagct gaaacacaaa cttgagcaac tgcggaatag ctgtgcatag
     1381 tgacgtacgg cggccgcctc gactagggat aacagggtaa ttgtttgaat gaggcttcag
     1441 tactttacag aatcgttgcc tgcacatctt ggaaacactt gctgggatta cttcgacttc
     1501 ttaacccaac agaaggctcg agaaggtata ttgctgttga cagtgagcga aaagatttag
     1561 ccataatgta atagtgaagc cacagatgta ttacattatg gctaaatctt tctgcctact
     1621 gcctcggact tcaaggggct agaattgaag gctcgagaag gtatattgct gttgacagtg
     1681 agcgcgggca taaaagaact tttttatagt gaagccacag atgtataaaa aagttctttt
     1741 atgcccatgc ctactgcctc ggacttcaag gggctagaat tgaaggctcg agaaggtata
     1801 ttgctgttga cagtgagcgc cacctatgaa cttgtttcaa atagtgaagc cacagatgta
     1861 tttgaaacaa gttcataggt gatgcctact gcctcggact tcaaggggct agaattcgag
     1921 caattatctt gtttactaaa actgaatacc ttgctatctc tttgatacat ttttacaaag
     1981 ctgaattaaa atggtataaa ttaaatcact tttttcaatt gacacgtacg cgtgatctaa
     2041 ttctactagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc
     2101 acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgatc cggtgcctag
     2161 agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg cctttttccc
     2221 gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct ttttcgcaac
     2281 gggtttgccg ccagaacaca ggaccggttg tagagcgctg ccaccatgac cgagtacaag
     2341 cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc
     2401 gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg
     2461 gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg
     2521 gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg
     2581 gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg
     2641 cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg
     2701 gccaccgtcg gagtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc
     2761 cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc
     2821 cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc
     2881 gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgaacgcc gccatcgata
     2941 aggagcgccc ctctccctcc ccccccccta acgttactgg ccgaagccgc ttggaataag
     3001 gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt ggcaatgtga
     3061 gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt tcccctctcg
     3121 ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt
     3181 gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca cctggcgaca
     3241 ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc
     3301 agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc tcaagcgtat
     3361 tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct gatctggggc
     3421 ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa
     3481 ccacggggac gtggttttcc tttgaaaaac acgatgataa tatggccaca accatgagca
     3541 gactggacaa gagcaaagtc ataaactctg ctctggaatt actcaatgga gtcggtatcg
     3601 aaggcctgac gacaaggaaa ctcgctcaaa agctgggagt tgagcagcct accctgtact
     3661 ggcacgtgaa gaacaagcgg gccctgctcg atgccctgcc aatcgagatg ctggacaggc
     3721 atcataccca ctcctgcccc ctggaaggcg agtcatggca agactttctg cggaacaacg
     3781 ccaagtcata ccgctgtgct ctcctctcac atcgcgacgg ggctaaagtg catctcggca
     3841 cccgcccaac agagaaacag tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc
     3901 aaggcttctc cctggagaac gcactgtacg ctctgtccgc cgtgggccac tttacactgg
     3961 gctgcgtatt ggaggaacag gagcatcaag tagcaaaaga ggaaagagag acacctacca
     4021 ccgattctat gcccccactt ctgaaacaag caattgagct gttcgaccgg cagggagccg
     4081 aacctgcctt ccttttcggc ctggaactaa tcatatgtgg cctggagaaa cagctaaagt
     4141 gcgaaagcgg cgggccgacc gacgcccttg acgattttga cttagacatg ctcccagccg
     4201 atgcccttga cgactttgac cttgatatgc tgcctgctga cgctcttgac gattttgacc
     4261 ttgacatgct ccccgggtaa gctctagagt taagtcgaca atcaacctct ggattacaaa
     4321 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4381 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4441 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4501 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4561 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4621 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4681 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     4741 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4801 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     4861 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     4921 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     4981 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     5041 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5101 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5161 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc
     5221 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg
     5281 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat
     5341 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag
     5401 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg
     5461 gaaagaacca gccattaatg aaaagaacca gccattaatg aatcggccaa cgcgcgggga
     5521 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     5581 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     5641 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     5701 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     5761 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     5821 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     5881 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     5941 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     6001 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     6061 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     6121 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
     6181 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     6241 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     6301 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     6361 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     6421 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     6481 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     6541 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     6601 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     6661 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     6721 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     6781 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     6841 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     6901 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     6961 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     7021 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     7081 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     7141 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     7201 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     7261 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     7321 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     7381 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     7441 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc
     7501 cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
     7561 tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac
     7621 aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta ggcgttttgc
     7681 gctgcttcgc gatgtacggg ccagatattg acattgatta ttgactagtt attaatagta
     7741 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
     7801 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
     7861 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
     7921 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
     7981 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
     8041 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
     8101 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
     8161 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
     8221 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
     8281 tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     8341 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     8401 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     8461 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     8521 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     8581 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     8641 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     8701 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     8761 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     8821 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     8881 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     8941 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     9001 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     9061 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     9121 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     9181 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     9241 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     9301 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     9361 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     9421 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     9481 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     9541 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     9601 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     9661 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     9721 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     9781 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     9841 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
     9901 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     9961 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
    10021 caaaattttc gggtttatta cagggacagc agagatccag tttggttaat taaggtaccg
    10081 ggaggtggtc cctgcagtta cgccaatgat aacccccgcc agaaaaatct tagtagcctt
    10141 ccctttttgt tttccgtgcc ccaactcggc ggattgactc ggccccttcc ggaaacaccc
    10201 gaatcaactt ctagtcaaat tattgttcac gccgcaatga cccacccctg gcccgcgtct
    10261 gtggaactga cccctggtgt acaggagagt tcgctgctga aagtggtccc aaaggggtac
    10321 tagtttttaa gctcccaact ccccctcccc cagcgtctgg aggattccac accctcgcac
    10381 cgcaggggcg aggaagtggg cggagtccgg ttttggcgcc agccgctgag gctgccaagc
    10441 agaaaagcca ccgctgagga gactccggtc actgtcctcg ccccgcctcc cccttccctc
    10501 cccttgggga ccaccgggcg ccacgccgcg aacggtaagt gccgcggtcg tcggcgcctc
    10561 cgccctcccc ctagggcccc aattcccagc gggcgcggcg cgcggccccg cgcgcagctc
    10621 ccggctccct cccccttcgg atgtggcttg agctgtaggc gcggaggcta gcctagattc
    10681 gagtttactc cctatcagtg atagagaacg tatgaagagt ttactcccta tcagtgatag
    10741 agaacgtatg cagactttac tccctatcag tgatagagaa cgtataagga gtttactccc
    10801 tatcagtgat agagaacgta tgaccagttt actccctatc agtgatagag aacgtatcta
    10861 cagtttactc cctatcagtg atagagaacg tatatccagt ttactcccta tcagtgatag
    10921 agaacgtatg tcgaggtagg cgtgtacggt gggcgcctat aaaagcagag ctcgtttagt
    10981 gaaccgtcag atcgcctgga gcaattccac aacacttttg tcttatactt
//
