LOCUS       Exported               11319 bp ds-DNA     circular SYN 10-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11319)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 10, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11319
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1
     source          1
                     /label=4188 to 3322 of pCF806-origin reset
                     /note="4188 to 3322 of pCF806-origin reset"
                     /note="/date=02-Apr-2024"
                     /note="/left_cutter=XhoI (4187)"
                     /note="/left_end=5TCGA"
                     /note="/right_cutter=BamHI (3322)"
                     /note="/right_end=5GATC"
                     /note="/user=Alain Nepveu"
     source          2190..7767
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label= [Split]
                     /note=" [Split]"
     source          7768..11319
     CDS             1..2189
                     /codon_start=1
                     /note="4188 to 3322 of pCF806-origin reset plus 2 to 2189 
                     of Untitled"
                     /note="/date=02-Apr-2024"
                     /note="/left_cutter=XhoI (4187)"
                     /note="/left_end=5TCGA"
                     /note="/left_join=keep end"
                     /note="/right_cutter=XhoI (2189)"
                     /note="/right_end=5TCGA"
                     /note="/right_join=keep end"
                     /note="/user=Alain Nepveu"
                     /translation="GSTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
                     LTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFF
                     KDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITLGMDELYKGSGMPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQ
                     QSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFST
                     ADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQA
                     ARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDS
                     SAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQA
                     PGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVR
                     VLRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKA
                     PKVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA*RTAAASTRD
                     NRVIV*MRLQYFTESLPAHLGNTCWDYFDFLTQQK"
     CDS             10..2055
                     /codon_start=1
                     /label=EGFP-MYC
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKGSGMPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSE
                     LQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQ
                     LEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARK
                     DSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAF
                     SPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGK
                     RSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLR
                     QISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKV
                     VILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     misc_feature    2379..2590
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor  
                     EF-1-alpha"
     CDS             2615..3214
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2615..2634)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also  
                     called puro-variant-R"
     primer_bind     3111..3131
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3237..3822
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the  
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3404..3421)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer.  
                     Also called pCDH-rev"
     primer_bind     3631..3650
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3823..4569
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to  
                     promoters containing the tet operator in the presence of  
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4589..5177
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional  
                     regulatory element"
     primer_bind     complement(4642..4662)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5060..5071)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5249..5482
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5508..5525)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer.  
                     Also called BGH reverse"
     misc_feature    5514..5738
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5844..5861)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      6015..6603
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
     primer_bind     complement(6095..6114)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6774..7634)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7397..7416
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_feature    complement(7635..7739)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7814..7833)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable  
                     marker"
     misc_feature    7999..8378
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    8380..8578
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
     primer_bind     8533..8553
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8596..8776
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8823..8948
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus  
                     type 1"
     misc_feature    9441..9674
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for  
                     Rev-dependent mRNA export from the nucleus to the  
                     cytoplasm."
     CDS             9859..9903
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids  
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik  
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10201..10318
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination  
                     sequence of HIV-1"
     misc_feature    10970..11319
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be  
                     activated by binding of Tet-On(R) 3G, optimized for  
                     retroviral and lentiviral vectors"
     protein_bind    10978..10996
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11014..11032
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11050..11068
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11086..11104
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11122..11140
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11158..11176
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    11194..11212
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     11258..11282
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggatccacca tggtgtctaa gggggaggag cttttcacgg gcgtcgtgcc catcttggta
       61 gaactggacg gcgacgtgaa tggccataag ttcagtgtgt ctggagaggg cgaaggcgat
      121 gcgacctacg ggaagttgac cctcaagttc atatgtacca caggcaagct gcccgtcccg
      181 tggccaacac tggtgactac cttgacttac ggcgttcaat gcttttctcg ctacccagac
      241 catatgaaac agcacgattt ttttaagtca gctatgcccg aggggtatgt gcaagagcgc
      301 acaatctttt tcaaggatga tgggaactac aagacgcgag cagaagtgaa attcgaaggt
      361 gatactttgg tgaataggat agaactgaag ggcatcgatt tcaaggagga cggaaacatc
      421 cttggccaca agttagagta taattacaat tcccacaacg tatatatcat ggctgacaaa
      481 cagaaaaacg ggatcaaagc aaacttcaaa attcgtcaca acatcgagga cgggagtgtc
      541 cagttagctg atcactatca gcagaacacc cctattggcg acggtcctgt cctgctgcct
      601 gacaaccatt acctgagcac tcagagtgca ctgtctaagg accctaatga aaagcgcgac
      661 catatggtgc tgttggagtt cgtcacagcc gccggaatta ccttgggtat ggatgagttg
      721 tacaagggca gtggaatgcc gctgaatgtt tcatttacta atcgcaatta tgatctggat
      781 tacgatagcg tgcagccgta cttttattgc gatgaggaag aaaacttcta ccagcagcag
      841 cagcagtccg agctccagcc tccagcccca agcgaggaca tctggaagaa gttcgagctg
      901 ttaccaaccc ctcctttgag cccaagtcgc agatccggtc tgtgttcacc ttcttatgtg
      961 gcggtcaccc cattctctct tcggggagac aatgacggag ggggggggtc tttcagtaca
     1021 gcagatcaac tggaaatggt caccgaactg ttgggcgggg acatggtgaa ccagtccttt
     1081 atttgcgatc cggatgacga gaccttcatt aaaaatatca ttattcagga ctgcatgtgg
     1141 tctggctttt ctgcggccgc aaagctggtt tctgagaagt tggcatccta ccaggcagcc
     1201 aggaaggact ccggcagccc taaccccgcc cggggccaca gtgtatgctc tacctctagt
     1261 ctctatctgc aagatctctc cgccgctgct agcgagtgca tcgatccttc cgtagtgttc
     1321 ccatatcctc tcaatgacag ctctagccct aagtcctgtg cctcacagga cagctctgct
     1381 tttagcccgt cttccgattc cctgctgtct tcaaccgaga gtagccccca agggagtcct
     1441 gagcccctcg tcctgcacga agagacacct ccgacgacta gcagcgactc tgaagaagag
     1501 caagaggatg aggaagagat tgacgtggtg agtgtggaga aaaggcaggc gcctggcaag
     1561 cgatctgagt ccggtagtcc atctgcaggc ggacatagca agccacctca ctctccattg
     1621 gttctcaagc ggtgccacgt gtccacgcat caacataatt acgccgcccc accctccacc
     1681 agaaaagact acccagccgc gaaaagagtg aagctggatt ccgtcagagt ccttcgtcaa
     1741 atctccaata accgcaaatg tacatcaccc cgctcctcag acacagaaga aaacgtaaaa
     1801 cggaggacac acaatgtcct tgagcggcag agaaggaacg agctgaagag gagctttttc
     1861 gccctgagag accagatccc tgagcttgag aataacgaga aggccccaaa agtggtcatc
     1921 ttaaagaagg ccacagctta tatcctgtca gtccaagctg aagaacagaa gttgatcagc
     1981 gaggaggacc ttctgcgcaa aagaagggaa cagctgaaac acaaactgga gcagctgaga
     2041 aattcttgtg cctaacgtac ggcggccgcc tcgactaggg ataacagggt aattgtttga
     2101 atgaggcttc agtactttac agaatcgttg cctgcacatc ttggaaacac ttgctgggat
     2161 tacttcgact tcttaaccca acagaaggct cgagcaacca gaattcgagc aattatcttg
     2221 tttactaaaa ctgaatacct tgctatctct ttgatacatt tttacaaagc tgaattaaaa
     2281 tggtataaat taaatcactt ttttcaattg acacgtacgc gtgatctaat tctactaggt
     2341 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     2401 agtccccgag aagttggggg gaggggtcgg caattgatcc ggtgcctaga gaaggtggcg
     2461 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     2521 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     2581 cagaacacag gaccggttgt agagcgctgc caccatgacc gagtacaagc ccacggtgcg
     2641 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     2701 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     2761 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     2821 cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     2881 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     2941 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     3001 agtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     3061 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     3121 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     3181 cacctggtgc atgacccgca agcccggtgc ctgaacgccg ccatcgataa ggagcgcccc
     3241 tctccctccc ccccccctaa cgttactggc cgaagccgct tggaataagg ccggtgtgcg
     3301 tttgtctata tgttattttc caccatattg ccgtcttttg gcaatgtgag ggcccggaaa
     3361 cctggccctg tcttcttgac gagcattcct aggggtcttt cccctctcgc caaaggaatg
     3421 caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg aagcttcttg aagacaaaca
     3481 acgtctgtag cgaccctttg caggcagcgg aaccccccac ctggcgacag gtgcctctgc
     3541 ggccaaaagc cacgtgtata agatacacct gcaaaggcgg cacaacccca gtgccacgtt
     3601 gtgagttgga tagttgtgga aagagtcaaa tggctctcct caagcgtatt caacaagggg
     3661 ctgaaggatg cccagaaggt accccattgt atgggatctg atctggggcc tcggtgcaca
     3721 tgctttacat gtgtttagtc gaggttaaaa aacgtctagg ccccccgaac cacggggacg
     3781 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatgagcag actggacaag
     3841 agcaaagtca taaactctgc tctggaatta ctcaatggag tcggtatcga aggcctgacg
     3901 acaaggaaac tcgctcaaaa gctgggagtt gagcagccta ccctgtactg gcacgtgaag
     3961 aacaagcggg ccctgctcga tgccctgcca atcgagatgc tggacaggca tcatacccac
     4021 tcctgccccc tggaaggcga gtcatggcaa gactttctgc ggaacaacgc caagtcatac
     4081 cgctgtgctc tcctctcaca tcgcgacggg gctaaagtgc atctcggcac ccgcccaaca
     4141 gagaaacagt acgaaaccct ggaaaatcag ctcgcgttcc tgtgtcagca aggcttctcc
     4201 ctggagaacg cactgtacgc tctgtccgcc gtgggccact ttacactggg ctgcgtattg
     4261 gaggaacagg agcatcaagt agcaaaagag gaaagagaga cacctaccac cgattctatg
     4321 cccccacttc tgaaacaagc aattgagctg ttcgaccggc agggagccga acctgccttc
     4381 cttttcggcc tggaactaat catatgtggc ctggagaaac agctaaagtg cgaaagcggc
     4441 gggccgaccg acgcccttga cgattttgac ttagacatgc tcccagccga tgcccttgac
     4501 gactttgacc ttgatatgct gcctgctgac gctcttgacg attttgacct tgacatgctc
     4561 cccgggtaag ctctagagtt aagtcgacaa tcaacctctg gattacaaaa tttgtgaaag
     4621 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     4681 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     4741 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     4801 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     4861 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     4921 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     4981 gaaatcatcg tcctttcctt ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     5041 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     5101 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     5161 ttgggccgcc tccccgcgtc gactttaaga ccaatgactt acaaggcagc tgtagatctt
     5221 agccactttt taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagacaa
     5281 gatctgcttt ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
     5341 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     5401 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     5461 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
     5521 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     5581 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     5641 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     5701 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
     5761 ccattaatga aaagaaccag ccattaatga atcggccaac gcgcggggag aggcggtttg
     5821 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     5881 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     5941 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     6001 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     6061 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     6121 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     6181 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
     6241 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     6301 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     6361 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     6421 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
     6481 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     6541 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     6601 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
     6661 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
     6721 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
     6781 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
     6841 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
     6901 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
     6961 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
     7021 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
     7081 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
     7141 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
     7201 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
     7261 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
     7321 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
     7381 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
     7441 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
     7501 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
     7561 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
     7621 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
     7681 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
     7741 acatttcccc gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat
     7801 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg
     7861 cttgtgtgtt ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag
     7921 gcttgaccga caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg
     7981 atgtacgggc cagatattga cattgattat tgactagtta ttaatagtaa tcaattacgg
     8041 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
     8101 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
     8161 tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta cggtaaactg
     8221 cccacttggc agtacatcaa gtgtatcata tgccaagtac gccccctatt gacgtcaatg
     8281 acggtaaatg gcccgcctgg cattatgccc agtacatgac cttatgggac tttcctactt
     8341 ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt tggcagtaca
     8401 tcaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac cccattgacg
     8461 tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt cgtaacaact
     8521 ccgccccatt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat ataagcagcg
     8581 cgttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8641 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8701 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     8761 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     8821 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     8881 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     8941 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     9001 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     9061 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     9121 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     9181 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     9241 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     9301 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     9361 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     9421 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     9481 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     9541 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     9601 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     9661 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     9721 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     9781 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     9841 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     9901 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     9961 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
    10021 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
    10081 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
    10141 gtgaacggat cggcactgcg tgcgccaatt ctgcagacaa atggcagtat tcatccacaa
    10201 ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag tagacataat
    10261 agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc aaaattttcg
    10321 ggtttattac agggacagca gagatccagt ttggttaatt aaggtaccgg gaggtggtcc
    10381 ctgcagttac gccaatgata acccccgcca gaaaaatctt agtagccttc cctttttgtt
    10441 ttccgtgccc caactcggcg gattgactcg gccccttccg gaaacacccg aatcaacttc
    10501 tagtcaaatt attgttcacg ccgcaatgac ccacccctgg cccgcgtctg tggaactgac
    10561 ccctggtgta caggagagtt cgctgctgaa agtggtccca aaggggtact agtttttaag
    10621 ctcccaactc cccctccccc agcgtctgga ggattccaca ccctcgcacc gcaggggcga
    10681 ggaagtgggc ggagtccggt tttggcgcca gccgctgagg ctgccaagca gaaaagccac
    10741 cgctgaggag actccggtca ctgtcctcgc cccgcctccc ccttccctcc ccttggggac
    10801 caccgggcgc cacgccgcga acggtaagtg ccgcggtcgt cggcgcctcc gccctccccc
    10861 tagggcccca attcccagcg ggcgcggcgc gcggccccgc gcgcagctcc cggctccctc
    10921 ccccttcgga tgtggcttga gctgtaggcg cggaggctag cctagattcg agtttactcc
    10981 ctatcagtga tagagaacgt atgaagagtt tactccctat cagtgataga gaacgtatgc
    11041 agactttact ccctatcagt gatagagaac gtataaggag tttactccct atcagtgata
    11101 gagaacgtat gaccagttta ctccctatca gtgatagaga acgtatctac agtttactcc
    11161 ctatcagtga tagagaacgt atatccagtt tactccctat cagtgataga gaacgtatgt
    11221 cgaggtaggc gtgtacggtg ggcgcctata aaagcagagc tcgtttagtg aaccgtcaga
    11281 tcgcctggag caattccaca acacttttgt cttatactt
//
