LOCUS       Exported               11061 bp ds-DNA     circular SYN 10-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11061)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 10, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11061
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     source          1
     source          1
                     /label=4188 to 3322 of pCF806-origin reset
                     /note="4188 to 3322 of pCF806-origin reset"
                     /note="/date=02-Apr-2024"
                     /note="/left_cutter=XhoI (4187)"
                     /note="/left_end=5TCGA"
                     /note="/right_cutter=BamHI (3322)"
                     /note="/right_end=5GATC"
                     /note="/user=Alain Nepveu"
     source          1932..7509
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /label= [Split]
                     /note=" [Split]"
     source          7510..11061
     CDS             1..1931
                     /codon_start=1
                     /note="4188 to 3322 of pCF806-origin reset plus 2 to 1931 
                     of Untitled"
                     /note="/date=02-Apr-2024"
                     /note="/left_cutter=XhoI (4187)"
                     /note="/left_end=5TCGA"
                     /note="/left_join=keep end"
                     /note="/right_cutter=XhoI (1931)"
                     /note="/right_end=5TCGA"
                     /note="/right_join=keep end"
                     /note="/user=Alain Nepveu"
                     /translation="GSTMVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGK
                     LTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFF
                     KDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKN
                     GIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM
                     VLLEFVTAAGITLGMDELYKGSGMPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQ
                     QSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFST
                     ADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQA
                     ARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDS
                     SAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQA
                     PGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVR
                     VLRQISNNRKCTSPRSSDTEEN*RTAAASTRDNRVIV*MRLQYFTESLPAHLGNTCWDY
                     FDFLTQQK"
     CDS             10..1797
                     /codon_start=1
                     /label=EGFP-MYCÆCTD
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYKGSGMPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSE
                     LQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQ
                     LEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARK
                     DSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAF
                     SPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGK
                     RSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLR
                     QISNNRKCTSPRSSDTEEN"
     misc_feature    2121..2332
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor  
                     EF-1-alpha"
     CDS             2357..2956
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2357..2376)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also  
                     called puro-variant-R"
     primer_bind     2853..2873
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2979..3564
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the  
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(3146..3163)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer.  
                     Also called pCDH-rev"
     primer_bind     3373..3392
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3565..4311
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to  
                     promoters containing the tet operator in the presence of  
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    4331..4919
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional  
                     regulatory element"
     primer_bind     complement(4384..4404)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4802..4813)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4991..5224
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(5250..5267)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer.  
                     Also called BGH reverse"
     misc_feature    5256..5480
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5586..5603)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      5757..6345
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
     primer_bind     complement(5837..5856)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6516..7376)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7139..7158
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_feature    complement(7377..7481)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7556..7575)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable  
                     marker"
     misc_feature    7741..8120
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    8122..8320
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
     primer_bind     8275..8295
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             8338..8518
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8565..8690
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus  
                     type 1"
     misc_feature    9183..9416
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for  
                     Rev-dependent mRNA export from the nucleus to the  
                     cytoplasm."
     CDS             9601..9645
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids  
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik  
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9943..10060
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination  
                     sequence of HIV-1"
     misc_feature    10712..11061
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be  
                     activated by binding of Tet-On(R) 3G, optimized for  
                     retroviral and lentiviral vectors"
     protein_bind    10720..10738
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10756..10774
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10792..10810
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10828..10846
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10864..10882
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10900..10918
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10936..10954
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     11000..11024
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggatccacca tggtttcaaa gggtgaagaa ttattcacag gggtggttcc gattctcgtg
       61 gaactcgacg gtgacgtgaa tggtcacaag ttttctgtct caggggaggg tgagggagat
      121 gccacatatg gcaaactgac cctcaaattc atttgtacca ctggcaaact gcctgtccca
      181 tggccaacat tggtcacaac ccttacttat ggagtgcagt gcttttctcg gtaccccgat
      241 catatgaagc agcacgactt tttcaaatca gccatgcctg aagggtacgt gcaggagagg
      301 acaatcttct ttaaagacga cggcaattat aagaccaggg ccgaagtgaa attcgagggc
      361 gatacattgg tcaaccgcat agagctgaag ggaattgatt ttaaggaaga cggcaacatt
      421 ctcggacaca agttagagta taattacaac agtcacaacg tgtatatcat ggctgacaag
      481 cagaaaaacg ggatcaaggc gaattttaag attagacata acattgaaga cggatcagtc
      541 cagcttgctg atcattatca gcagaatacc cccataggcg atgggccagt tctgttgcct
      601 gataatcact accttagcac acaatcagcc ctcagcaagg accctaacga gaagagagat
      661 catatggttc tgctcgaatt tgtcaccgca gccggtatta ctctgggaat ggatgagctc
      721 tacaaaggct cagggatgcc tctgaatgtg agcttcacaa atcggaatta cgatctggac
      781 tacgattcag ttcagcctta cttttactgt gacgaagagg aaaactttta ccaacaacaa
      841 cagcaaagcg agttgcagcc acctgccccc tcagaagaca tttggaagaa attcgaactg
      901 ctgcctacgc cccccctgag ccctagccgc cggagtggcc tttgtagtcc atcctacgtt
      961 gcagtcaccc ctttttcatt gcgaggcgat aacgatggag ggggagggag tttctctacc
     1021 gcagaccagc ttgagatggt gaccgagctc ctgggagggg acatggttaa tcagtccttc
     1081 atttgtgacc cagatgatga gacatttata aagaacatca tcattcagga ttgtatgtgg
     1141 agtggattct ccgccgccgc caagctcgtg tccgagaaat tggcatccta ccaggctgcc
     1201 cggaaggact ctggcagccc caacccagcc cgtggccata gcgtgtgctc aacctccagc
     1261 ttgtacctgc aggacctcag tgctgcggca agcgaatgca tcgatccatc cgtggtgttt
     1321 ccctaccccc ttaacgatag ctctagtcct aaaagctgcg catctcagga tagcagtgcc
     1381 ttctcaccat cttctgatag ccttctgagt tccactgaga gttcacctca aggctctcca
     1441 gagcctctcg tcttacacga ggaaaccccc cccactacat cctctgactc cgaagaagaa
     1501 caggaagacg aagaagaaat tgatgtagta agcgttgaga aacgacaggc gccggggaaa
     1561 cgatcagaat ctgggtcacc ttctgcaggt ggccactcaa agccacccca ttccccactg
     1621 gtgctcaagc gatgccacgt gagtacgcac cagcataatt atgccgctcc accctcaacc
     1681 cggaaggatt atccagctgc caagcgagtc aagctggact ctgtccgagt tttgaggcaa
     1741 attagcaata atcgcaagtg cactagccct agatcatctg acaccgagga gaattagcgt
     1801 acggcggccg cctcgactag ggataacagg gtaattgttt gaatgaggct tcagtacttt
     1861 acagaatcgt tgcctgcaca tcttggaaac acttgctggg attacttcga cttcttaacc
     1921 caacagaagg ctcgagcaac cagaattcga gcaattatct tgtttactaa aactgaatac
     1981 cttgctatct ctttgataca tttttacaaa gctgaattaa aatggtataa attaaatcac
     2041 ttttttcaat tgacacgtac gcgtgatcta attctactag gtcttgaaag gagtgggaat
     2101 tggctccggt gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg
     2161 gggaggggtc ggcaattgat ccggtgccta gagaaggtgg cgcggggtaa actgggaaag
     2221 tgatgtcgtg tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc
     2281 agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggaccggtt
     2341 gtagagcgct gccaccatga ccgagtacaa gcccacggtg cgcctcgcca cccgcgacga
     2401 cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc gactaccccg ccacgcgcca
     2461 caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag ctgcaagaac tcttcctcac
     2521 gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac gacggcgccg cggtggcggt
     2581 ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc gccgagatcg gcccgcgcat
     2641 ggccgagttg agcggttccc ggctggccgc gcagcaacag atggaaggcc tcctggcgcc
     2701 gcaccggccc aaggagcccg cgtggttcct ggccaccgtc ggagtctcgc ccgaccacca
     2761 gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg gaggcggccg agcgcgccgg
     2821 ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc cccttctacg agcggctcgg
     2881 cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg cgcacctggt gcatgacccg
     2941 caagcccggt gcctgaacgc cgccatcgat aaggagcgcc cctctccctc ccccccccct
     3001 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
     3061 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
     3121 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
     3181 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
     3241 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
     3301 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
     3361 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
     3421 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
     3481 tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa
     3541 cacgatgata atatggccac aaccatgagc agactggaca agagcaaagt cataaactct
     3601 gctctggaat tactcaatgg agtcggtatc gaaggcctga cgacaaggaa actcgctcaa
     3661 aagctgggag ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc
     3721 gatgccctgc caatcgagat gctggacagg catcataccc actcctgccc cctggaaggc
     3781 gagtcatggc aagactttct gcggaacaac gccaagtcat accgctgtgc tctcctctca
     3841 catcgcgacg gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc
     3901 ctggaaaatc agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac
     3961 gctctgtccg ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa
     4021 gtagcaaaag aggaaagaga gacacctacc accgattcta tgcccccact tctgaaacaa
     4081 gcaattgagc tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta
     4141 atcatatgtg gcctggagaa acagctaaag tgcgaaagcg gcgggccgac cgacgccctt
     4201 gacgattttg acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg
     4261 ctgcctgctg acgctcttga cgattttgac cttgacatgc tccccgggta agctctagag
     4321 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     4381 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     4441 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     4501 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     4561 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     4621 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     4681 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     4741 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     4801 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     4861 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     4921 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     4981 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     5041 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     5101 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     5161 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     5221 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     5281 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     5341 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     5401 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     5461 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agccattaat gaaaagaacc
     5521 agccattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
     5581 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
     5641 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
     5701 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
     5761 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
     5821 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
     5881 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
     5941 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
     6001 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
     6061 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
     6121 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
     6181 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
     6241 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
     6301 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
     6361 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
     6421 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
     6481 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
     6541 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
     6601 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
     6661 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
     6721 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
     6781 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
     6841 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
     6901 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
     6961 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
     7021 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
     7081 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat
     7141 aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg
     7201 cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca
     7261 cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga
     7321 aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc
     7381 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata
     7441 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg
     7501 ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa
     7561 tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg
     7621 ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca
     7681 tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatatt
     7741 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     7801 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     7861 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     7921 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7981 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     8041 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     8101 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     8161 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     8221 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     8281 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     8341 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8401 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8461 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8521 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     8581 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     8641 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     8701 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     8761 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     8821 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     8881 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     8941 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     9001 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     9061 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     9121 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     9181 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     9241 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     9301 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     9361 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     9421 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     9481 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     9541 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     9601 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     9661 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     9721 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     9781 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     9841 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     9901 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     9961 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
    10021 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag
    10081 cagagatcca gtttggttaa ttaaggtacc gggaggtggt ccctgcagtt acgccaatga
    10141 taacccccgc cagaaaaatc ttagtagcct tccctttttg ttttccgtgc cccaactcgg
    10201 cggattgact cggccccttc cggaaacacc cgaatcaact tctagtcaaa ttattgttca
    10261 cgccgcaatg acccacccct ggcccgcgtc tgtggaactg acccctggtg tacaggagag
    10321 ttcgctgctg aaagtggtcc caaaggggta ctagttttta agctcccaac tccccctccc
    10381 ccagcgtctg gaggattcca caccctcgca ccgcaggggc gaggaagtgg gcggagtccg
    10441 gttttggcgc cagccgctga ggctgccaag cagaaaagcc accgctgagg agactccggt
    10501 cactgtcctc gccccgcctc ccccttccct ccccttgggg accaccgggc gccacgccgc
    10561 gaacggtaag tgccgcggtc gtcggcgcct ccgccctccc cctagggccc caattcccag
    10621 cgggcgcggc gcgcggcccc gcgcgcagct cccggctccc tcccccttcg gatgtggctt
    10681 gagctgtagg cgcggaggct agcctagatt cgagtttact ccctatcagt gatagagaac
    10741 gtatgaagag tttactccct atcagtgata gagaacgtat gcagacttta ctccctatca
    10801 gtgatagaga acgtataagg agtttactcc ctatcagtga tagagaacgt atgaccagtt
    10861 tactccctat cagtgataga gaacgtatct acagtttact ccctatcagt gatagagaac
    10921 gtatatccag tttactccct atcagtgata gagaacgtat gtcgaggtag gcgtgtacgg
    10981 tgggcgccta taaaagcaga gctcgtttag tgaaccgtca gatcgcctgg agcaattcca
    11041 caacactttt gtcttatact t
//
