LOCUS       Exported               10410 bp ds-DNA     circular SYN 10-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 10, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1,1281..10410)
                     /organism="synthetic DNA construct"
     CDS             1..1280
                     /codon_start=1
                     /note="1 to 9131 of pCF806 M80 (mCherry No EcRI) Vector 
                     plus 2 to 1280 of pTwistAmp M79"
                     /note="/date=29-Apr-2024"
                     /note="/left_cutter=XhoI (9984)"
                     /note="/left_end=5TCGA"
                     /note="/left_join=keep end"
                     /note="/right_cutter=XhoI (1280)"
                     /note="/right_end=5TCGA"
                     /note="/right_join=keep end"
                     /translation="GSTMGKPIPNPLLGLDSTGSGKKKKSGSMPLNVSFTNRNYDLDYD
                     SVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVA
                     VTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMW
                     SGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVV
                     FPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSE
                     EEQEDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAP
                     PSTRKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEEN*RTAAASTRDNRVIV*MR
                     LQYFTESLPAHLGNTCWDYFDFLTQQK"
     CDS             1
                     /codon_start=1
                     /note="4419 to 3553 of pCF806 Vector plus 2 to 854 of 
                     pTwistAmp M80 mCherry No EcoRI [Split]"
                     /note="/date=29-Apr-2024"
                     /note="/left_cutter=XhoI (4418)"
                     /note="/left_end=5TCGA"
                     /note="/left_join=keep end"
                     /note="/right_cutter=XhoI (854)"
                     /note="/right_end=5TCGA"
                     /note="/right_join=keep end"
                     /translation=""
     CDS             1
                     /codon_start=1
                     /label=1 to 9131 of pCF806 M80 (mCherry No EcRI) Vector
                     /note="1 to 9131 of pCF806 M80 (mCherry No EcRI) Vector"
                     /note="/date=29-Apr-2024"
                     /note="/left_cutter=XhoI (9984)"
                     /note="/left_end=5TCGA"
                     /note="/right_cutter=BamHI (9131)"
                     /note="/right_end=5GATC"
                     /translation=""
     CDS             10..1146
                     /codon_start=1
                     /label=V5-KKKK-MYCÆCTD
                     /translation="MGKPIPNPLLGLDSTGSGKKKKSGSMPLNVSFTNRNYDLDYDSVQ
                     PYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTP
                     FSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGF
                     SAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPY
                     PLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQ
                     EDEEEIDVVSVEKRQAPGKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPST
                     RKDYPAAKRVKLDSVRVLRQISNNRKCTSPRSSDTEEN"
     misc_feature    1470..1681
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor  
                     EF-1-alpha"
     CDS             1706..2305
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1706..1725)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also  
                     called puro-variant-R"
     primer_bind     2202..2222
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2328..2913
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the  
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2495..2512)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer.  
                     Also called pCDH-rev"
     primer_bind     2722..2741
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2914..3660
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to  
                     promoters containing the tet operator in the presence of  
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3680..4268
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional  
                     regulatory element"
     primer_bind     complement(3733..3753)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4151..4162)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4340..4573
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4599..4616)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer.  
                     Also called BGH reverse"
     misc_feature    4605..4829
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(4935..4952)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      5106..5694
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
     primer_bind     complement(5186..5205)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(5865..6725)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6488..6507
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_feature    complement(6726..6830)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(6905..6924)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable  
                     marker"
     misc_feature    7090..7469
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    7471..7669
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
     primer_bind     7624..7644
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7687..7867
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7914..8039
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus  
                     type 1"
     misc_feature    8532..8765
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for  
                     Rev-dependent mRNA export from the nucleus to the  
                     cytoplasm."
     CDS             8950..8994
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids  
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik  
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9292..9409
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination  
                     sequence of HIV-1"
     misc_feature    10061..10410
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be  
                     activated by binding of Tet-On(R) 3G, optimized for  
                     retroviral and lentiviral vectors"
     protein_bind    10069..10087
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10105..10123
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10141..10159
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10177..10195
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10213..10231
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10249..10267
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10285..10303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10349..10373
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggatccacca tggggaagcc tatcccaaat ccactgttgg gcttggacag taccggcagc
       61 ggaaaaaaaa aaaaatctgg atcaatgccg cttaacgtgt ctttcactaa ccgaaattac
      121 gatcttgact acgattccgt ccagccttat ttctattgtg acgaagagga aaacttttac
      181 cagcagcagc agcagagcga gcttcagcct ccagccccct ccgaagatat ctggaagaag
      241 ttcgagttgc tgcctacccc cccccttagt ccttctcgac ggtctggtct ctgttcccca
      301 tcctatgttg cagtgactcc attttcactc cgcggggaca atgacggtgg aggtggcagc
      361 ttctcaacgg cggatcagtt agagatggtc actgaactgc ttgggggcga tatggtgaac
      421 cagagtttca tttgcgaccc cgacgacgag acttttatca aaaatatcat tattcaggat
      481 tgcatgtggt ccggcttttc cgcagctgcg aagctcgtgt cagagaagtt agcttcttac
      541 caggccgcac ggaaggatag cggatctccg aacccggcta ggggtcactc tgtgtgttct
      601 accagctcat tgtaccttca ggaccttagc gctgccgcta gcgagtgcat tgacccttcc
      661 gtggtgtttc cataccccct taacgactca agttcaccca aaagttgcgc ctcccaggac
      721 tctagtgctt ttagtcctag tagtgacagt ttactctcct ccactgagag ctccccccag
      781 gggtctcctg aacccctggt cctgcatgag gagacccccc ctacaacttc ttcagacagc
      841 gaagaagaac aggaagatga agaagagatc gacgtggtgt ctgtggaaaa acgacaagcc
      901 cctgggaaaa gaagcgagtc tgggtccccg agtgccgggg ggcattccaa accaccccat
      961 tcacccctgg tcctcaagcg gtgccacgtg tccactcatc aacacaatta tgcggctccc
     1021 ccaagcacta ggaaggatta tcctgctgcc aaacgggtta agctggactc cgtccgagtc
     1081 ctgcggcaga ttagcaataa caggaaatgc acctctccca gaagctccga tactgaagaa
     1141 aactgacgta cggcggccgc ctcgactagg gataacaggg taattgtttg aatgaggctt
     1201 cagtacttta cagaatcgtt gcctgcacat cttggaaaca cttgctggga ttacttcgac
     1261 ttcttaaccc aacagaaggc tcgagcaacc agaattcgag caattatctt gtttactaaa
     1321 actgaatacc ttgctatctc tttgatacat ttttacaaag ctgaattaaa atggtataaa
     1381 ttaaatcact tttttcaatt gacacgtacg cgtgatctaa ttctactagg tcttgaaagg
     1441 agtgggaatt ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga
     1501 gaagttgggg ggaggggtcg gcaattgatc cggtgcctag agaaggtggc gcggggtaaa
     1561 ctgggaaagt gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta
     1621 tataagtgca gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca
     1681 ggaccggttg tagagcgctg ccaccatgac cgagtacaag cccacggtgc gcctcgccac
     1741 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
     1801 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
     1861 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
     1921 ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
     1981 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
     2041 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gagtctcgcc
     2101 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
     2161 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
     2221 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
     2281 catgacccgc aagcccggtg cctgaacgcc gccatcgata aggagcgccc ctctccctcc
     2341 ccccccccta acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat
     2401 atgttatttt ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct
     2461 gtcttcttga cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg
     2521 ttgaatgtcg tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta
     2581 gcgacccttt gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag
     2641 ccacgtgtat aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg
     2701 atagttgtgg aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat
     2761 gcccagaagg taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca
     2821 tgtgtttagt cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc
     2881 tttgaaaaac acgatgataa tatggccaca accatgagca gactggacaa gagcaaagtc
     2941 ataaactctg ctctggaatt actcaatgga gtcggtatcg aaggcctgac gacaaggaaa
     3001 ctcgctcaaa agctgggagt tgagcagcct accctgtact ggcacgtgaa gaacaagcgg
     3061 gccctgctcg atgccctgcc aatcgagatg ctggacaggc atcataccca ctcctgcccc
     3121 ctggaaggcg agtcatggca agactttctg cggaacaacg ccaagtcata ccgctgtgct
     3181 ctcctctcac atcgcgacgg ggctaaagtg catctcggca cccgcccaac agagaaacag
     3241 tacgaaaccc tggaaaatca gctcgcgttc ctgtgtcagc aaggcttctc cctggagaac
     3301 gcactgtacg ctctgtccgc cgtgggccac tttacactgg gctgcgtatt ggaggaacag
     3361 gagcatcaag tagcaaaaga ggaaagagag acacctacca ccgattctat gcccccactt
     3421 ctgaaacaag caattgagct gttcgaccgg cagggagccg aacctgcctt ccttttcggc
     3481 ctggaactaa tcatatgtgg cctggagaaa cagctaaagt gcgaaagcgg cgggccgacc
     3541 gacgcccttg acgattttga cttagacatg ctcccagccg atgcccttga cgactttgac
     3601 cttgatatgc tgcctgctga cgctcttgac gattttgacc ttgacatgct ccccgggtaa
     3661 gctctagagt taagtcgaca atcaacctct ggattacaaa atttgtgaaa gattgactgg
     3721 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     3781 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     3841 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     3901 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     3961 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4021 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     4081 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4141 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     4201 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     4261 ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct tagccacttt
     4321 ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca agatctgctt
     4381 tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa
     4441 ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt
     4501 gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg
     4561 aaaatctcta gcagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg
     4621 ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc
     4681 cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc
     4741 tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag
     4801 gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gccattaatg
     4861 aaaagaacca gccattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     4921 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     4981 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     5041 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     5101 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     5161 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     5221 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     5281 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     5341 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     5401 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     5461 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     5521 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     5581 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     5641 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     5701 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     5761 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     5821 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     5881 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
     5941 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
     6001 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
     6061 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
     6121 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
     6181 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
     6241 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
     6301 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
     6361 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
     6421 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
     6481 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
     6541 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
     6601 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
     6661 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
     6721 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
     6781 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
     6841 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
     6901 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
     6961 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
     7021 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
     7081 ccagatattg acattgatta ttgactagtt attaatagta atcaattacg gggtcattag
     7141 ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     7201 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     7261 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     7321 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     7381 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     7441 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     7501 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     7561 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     7621 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcagc gcgttttgcc
     7681 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
     7741 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
     7801 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
     7861 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
     7921 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     7981 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     8041 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     8101 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     8161 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     8221 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     8281 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     8341 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     8401 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     8461 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     8521 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     8581 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     8641 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     8701 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     8761 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     8821 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     8881 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     8941 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     9001 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     9061 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     9121 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     9181 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     9241 tcggcactgc gtgcgccaat tctgcagaca aatggcagta ttcatccaca attttaaaag
     9301 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     9361 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     9421 cagggacagc agagatccag tttggttaat taaggtaccg ggaggtggtc cctgcagtta
     9481 cgccaatgat aacccccgcc agaaaaatct tagtagcctt ccctttttgt tttccgtgcc
     9541 ccaactcggc ggattgactc ggccccttcc ggaaacaccc gaatcaactt ctagtcaaat
     9601 tattgttcac gccgcaatga cccacccctg gcccgcgtct gtggaactga cccctggtgt
     9661 acaggagagt tcgctgctga aagtggtccc aaaggggtac tagtttttaa gctcccaact
     9721 ccccctcccc cagcgtctgg aggattccac accctcgcac cgcaggggcg aggaagtggg
     9781 cggagtccgg ttttggcgcc agccgctgag gctgccaagc agaaaagcca ccgctgagga
     9841 gactccggtc actgtcctcg ccccgcctcc cccttccctc cccttgggga ccaccgggcg
     9901 ccacgccgcg aacggtaagt gccgcggtcg tcggcgcctc cgccctcccc ctagggcccc
     9961 aattcccagc gggcgcggcg cgcggccccg cgcgcagctc ccggctccct cccccttcgg
    10021 atgtggcttg agctgtaggc gcggaggcta gcctagattc gagtttactc cctatcagtg
    10081 atagagaacg tatgaagagt ttactcccta tcagtgatag agaacgtatg cagactttac
    10141 tccctatcag tgatagagaa cgtataagga gtttactccc tatcagtgat agagaacgta
    10201 tgaccagttt actccctatc agtgatagag aacgtatcta cagtttactc cctatcagtg
    10261 atagagaacg tatatccagt ttactcccta tcagtgatag agaacgtatg tcgaggtagg
    10321 cgtgtacggt gggcgcctat aaaagcagag ctcgtttagt gaaccgtcag atcgcctgga
    10381 gcaattccac aacacttttg tcttatactt
//
