LOCUS       Exported               10611 bp ds-DNA     circular SYN 10-JUL-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Jul 10, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          join(1,773..1376,1482..10611)
                     /organism="synthetic DNA construct"
     CDS             1..1376
                     /codon_start=1
                     /note="9880 to 9131 of pCF806 M80 (mCherry No EcRI) Vector 
                     plus 2937 to 604 of pTwistAmp M75"
                     /note="/date=03-Oct-2024"
                     /note="/left_cutter=SceI (9879)"
                     /note="/left_end=3ATAA"
                     /note="/left_join=keep end"
                     /note="/right_cutter=SceI (604)"
                     /note="/right_end=3ATAA"
                     /note="/right_join=keep end"
                     /translation="GSTMGKPIPNPLLGLDSTGSGKKKKSGSMPLNVSFTNRNYDLDYD
                     SVQPYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVA
                     VTPFSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMW
                     SGFSAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVV
                     FPYPLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSDSEEEQEDEEEIDVVSVEKRQAP
                     GKRSESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRV
                     LRQISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAP
                     KVVILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA**RTAAASTRD
                     "
     CDS             1
                     /codon_start=1
                     /note="9880 to 9131 of pCF806 M80 (mCherry No EcRI) Vector"
                     /note="/date=03-Oct-2024"
                     /note="/left_cutter=SceI (9879)"
                     /note="/left_end=3ATAA"
                     /note="/right_cutter=BamHI (9131)"
                     /note="/right_end=5GATC"
                     /translation=""
     CDS             1
                     /codon_start=1
                     /translation=""
     CDS             1
                     /codon_start=1
                     /label=4419 to 3553 of pCF806 Vector
                     /note="4419 to 3553 of pCF806 Vector"
                     /note="/date=29-Apr-2024"
                     /note="/left_cutter=XhoI (4418)"
                     /note="/left_end=5TCGA"
                     /note="/right_cutter=BamHI (3553)"
                     /note="/right_end=5GATC"
                     /translation=""
     CDS             10..1344
                     /codon_start=1
                     /label=V5-KKKK-MYC
                     /translation="MGKPIPNPLLGLDSTGSGKKKKSGSMPLNVSFTNRNYDLDYDSVQ
                     PYFYCDEEENFYQQQQQSELQPPAPSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTP
                     FSLRGDNDGGGGSFSTADQLEMVTELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGF
                     SAAAKLVSEKLASYQAARKDSGSPNPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPY
                     PLNDSSSPKSCASQDSSAFSPSSDSLLSSTESSDSEEEQEDEEEIDVVSVEKRQAPGKR
                     SESGSPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQ
                     ISNNRKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVV
                     ILKKATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     CDS             1482..10611
                     /codon_start=1
                     /translation="SSNQNSSNYLVY*N*IPCYLFDTFLQS*IKMV*IKSLFSIDTYA*
                     SNSTRS*KEWELAPVPVSGQSAHRPQSPRSWGEGSAIDPVPREGGAG*TGKVMSCTGSA
                     FFPRVGENRI*VQ*SP*TFFFATGLPPEHRTGCRALPP*PSTSPRCASPPATTSPGPYA
                     PSPPRSPTTPPRATPSIRTATSSGSPSCKNSSSRASGSTSARCGSRTTAPRWRSGPRRR
                     ASKRGRCSPRSARAWPS*AVPGWPRSNRWKASWRRTGPRSPRGSWPPSESRPTTRARVW
                     AAPSCSPEWRRPSAPGCPPSWRPPRPATSPSTSGSASPSPPTSRCPKDRAPGA*PASPV
                     PERRHR*GAPLSLPPP*RYWPKPLGIRPVCVCLYVIFHHIAVFWQCEGPETWPCLLDEH
                     S*GSFPSRQRNARSVECREGSSSSGSFLKTNNVCSDPLQAAEPPTWRQVPLRPKATCIR
                     YTCKGGTTPVPRCELDSCGKSQMALLKRIQQGAEGCPEGTPLYGI*SGASVHMLYMCLV
                     EVKKRLGPPNHGDVVFL*KTR**YGHNHEQTGQEQSHKLCSGITQWSRYRRPDDKETRS
                     KAGS*AAYPVLAREEQAGPARCPANRDAGQASYPLLPPGRRVMARLSAEQRQVIPLCSP
                     LTSRRG*SASRHPPNRETVRNPGKSARVPVSARLLPGERTVRSVRRGPLYTGLRIGGTG
                     ASSSKRGKRDTYHRFYAPTSETSN*AVRPAGSRTCLPFRPGTNHMWPGETAKVRKRRAD
                     RRP*RF*LRHAPSRCP*RL*P*YAAC*RS*RF*P*HAPRVSSRVKSTINLWITKFVKD*
                     LVFLTMLLLLRYVDTLL*CLCIMLLLPVWLSFSPPCINPGCCLFMRSCGPLSGNVAWCA
                     LCLLTQPPLVGALPPPVSSFPGLSLSPSLLPRRNSSPPALPAAGQGLGCWALTIPWCCR
                     GNHRPFLGCSPVLPPGFCAGRPSATSLRPSIQRTFLPAACCRLCGLFRVFAFALRRVGS
                     PFGPPPRVDFKTNDLQGSCRS*PLFKRKGGTGRANSLPTKTRSAFCLYWVSLVRPDLSL
                     GALWLTREPTA*ASIKLALSASSSVCPSVV*LW*LEIPQTLLVSVENL*QGPFKPADQP
                     RLCLLVASHLLFAPPPCLP*PWKVPLPLSFPNKMRKLHRIV*VGVILFWGVGWGRTARG
                     RIGKTIAGMLGMRWALWLLRRKEPAINEKNQPLMNRPTRGERRFAYWALFRFLAH*LAA
                     LGRSAAASGISSLKGGNTVIHRIRG*RRKEHVSKRPAKGQEP*KGRVAGVFP*APPP*R
                     ASQKSTLKSEVAKPDRTIKIPGVSPWKLPRALSCSDPAAYRIPVRLSPFGKRGAFS*LT
                     L*VSQFGVGRSLQAGLCARTPRSARPLRLIR*LSS*VQPGKTRLIATGSSHW*QD*QSE
                     VCRRCYRVLEVVA*LRLH*KNSIWYLRSAEASYLRKKSW*LLIRQTNHRW*RWFFCLQA
                     ADYAQKKRISRRSFDLFYGV*RSVERKLTLRDFGHEIIKKDLHLDPFKLKMKF*INLKY
                     I*VNLV*QLPMLNQ*GTYLSDLSISFIHSCLTPRRVDNYDTGGLTIWPQCCNDTARPTL
                     TGSRFISNKPASRKGRAQKWSCNFIRLHPVY*LLPGS*SK*FAS**FAQRCCHCYRHRG
                     VTLVVWYGFIQLRFPTIKASYMIPHVVQKSG*LLRSSDRCQK*VGRSVITHGYGSTA*F
                     SYCHAIRKMLFCDW*VLNQVILRIVYAATELLLPGVNTG*YRAT*QNFKSAHHWKTFFG
                     AKTLKDLTAVEIQFDVTHSCTQLIFSIFYFHQRFWVSKNRKAKCRKKGNKGDTEMLNTH
                     TLPFSILLKHLSGLLSHERIHI*MYLEK*TNRGSAHISPKSAT*RRRIGRSPDPLWCTL
                     STICSDAA*LSQYLLPACVLEVAE*CASKI*ATTRQGLTDNCMKNLLRVRRFALLRDVR
                     ARY*H*LLTSY***SITGSLVHSPYMEFRVT*LTVNGPPG*PPNDPRPLTSIMTYVPIV
                     TPIGTFH*RQWVEYLR*TAHLAVHQVYHMPSTPPIDVNDGKWPAWHYAQYMTLWDFPTW
                     QYIYVLVIAITMVMRFWQYINGRG*RFDSRGFPSLHPIDVNGSLFWHQNQRDFPKCRNN
                     SAPLTQMGGRRVRWEVYISSAFCLYWVSLVRPDLSLGALWLTREPTA*ASIKLALSASS
                     SVCPSVV*LW*LEIPQTLLVSVENL*QWRPNRDLKAKGKPEELSRRRTRLAEARTARGE
                     GRRLVSTPKILTSGG*KERDGCESVSIKRGRIRSRWEKIRLRPGGKKKYKLKHIVWASR
                     ELERFAVNPGLLETSEGCRQILGQLQPSLQTGSEELRSLYNTVATLYCVHQRIEIKDTK
                     EALDKIEEEQNKSKTTAQQAAADLQTWRRRYEGQLEK*II*I*SSKN*TIRSSTHQGKE
                     KSGAERKKSSGNRSFVPWVLGSSRKHYGRSVNDADGTGQTIIVWYSAAAEQFAEGY*GA
                     TASVATHSLGHQAAPGKNPGCGKIPKGSTAPGDLGLLWKTHLHHCCALEC*LE**ISGT
                     DLESHDLDGVGQRN*QLHKLNTLLN*RIAKPARKE*TRIIGIR*MGKFVELV*HNKLAV
                     VYKIIHNDSRRLGRFKNSFCCTFYSE*S*AGIFTIIVSDPPPNPEGTRQARRNRRRRWR
                     ERQRQIHSISERIGTACANSADKWQYSSTILKEKGGLGGTVQGKE**T**QQTYKLKNY
                     KNKLQKFKIFGFITGTAEIQFG*LRYREVVPAVTPMITPARKILVAFPFCFPCPNSAD*
                     LGPFRKHPNQLLVKLLFTPQ*PTPGPRLWN*PLVYRRVRC*KWSQRGTSF*APNSPSPS
                     VWRIPHPRTAGARKWAESGFGASR*GCQAEKPPLRRLRSLSSPRLPLPSPWGPPGATPR
                     TVSAAVVGASALPLGPQFPAGAARGPARSSRLPPPSDVA*AVGAEASLDSSLLPISDRE
                     RMKSLLPISDRERMQTLLPISDRERIRSLLPISDRERMTSLLPISDRERIYSLLPISDR
                     ERISSLLPISDRERMSR*ACTVGAYKSRARLVNRQIAWSNSTTLLSYT"
     misc_feature    1671..1882
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor  
                     EF-1-alpha"
     CDS             1907..2506
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(1907..1926)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also  
                     called puro-variant-R"
     primer_bind     2403..2423
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2529..3114
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the  
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2696..2713)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer.  
                     Also called pCDH-rev"
     primer_bind     2923..2942
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3115..3861
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to  
                     promoters containing the tet operator in the presence of  
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     misc_feature    3881..4469
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional  
                     regulatory element"
     primer_bind     complement(3934..3954)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4352..4363)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4541..4774
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(4800..4817)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer.  
                     Also called BGH reverse"
     misc_feature    4806..5030
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(5136..5153)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      5307..5895
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of  
                     replication"
     primer_bind     complement(5387..5406)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(6066..6926)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6689..6708
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     misc_feature    complement(6927..7031)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7106..7125)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable  
                     marker"
     misc_feature    7291..7670
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     misc_feature    7672..7870
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early  
                     promoter"
     primer_bind     7825..7845
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             7888..8068
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8115..8240
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus  
                     type 1"
     misc_feature    8733..8966
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for  
                     Rev-dependent mRNA export from the nucleus to the  
                     cytoplasm."
     CDS             9151..9195
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids  
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik  
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9493..9610
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination  
                     sequence of HIV-1"
     misc_feature    10262..10611
                     /label=TRE3GV promoter
                     /note="3rd-generation Tet-responsive promoter that can be  
                     activated by binding of Tet-On(R) 3G, optimized for  
                     retroviral and lentiviral vectors"
     protein_bind    10270..10288
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10306..10324
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10342..10360
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10378..10396
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10414..10432
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10450..10468
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10486..10504
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10550..10574
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 ggatccacca tggggaagcc tatcccaaat ccactgttgg gcttggacag taccggcagc
       61 ggaaaaaaaa aaaaatctgg atcaatgccg cttaacgtgt ctttcactaa ccgaaattac
      121 gatcttgact acgattccgt ccagccttat ttctattgtg acgaagagga aaacttttac
      181 cagcagcagc agcagagcga gcttcagcct ccagccccct ccgaagatat ctggaagaag
      241 ttcgagttgc tgcctacccc cccccttagt ccttctcgac ggtctggtct ctgttcccca
      301 tcctatgttg cagtgactcc attttcactc cgcggggaca atgacggtgg aggtggcagc
      361 ttctcaacgg cggatcagtt agagatggtc actgaactgc ttgggggcga tatggtgaac
      421 cagagtttca tttgcgaccc cgacgacgag acttttatca aaaatatcat tattcaggat
      481 tgcatgtggt ccggcttttc cgcagctgcg aagctcgtgt cagagaagtt agcttcttac
      541 caggccgcac ggaaggatag cggatctccg aacccggcta ggggtcactc tgtgtgttct
      601 accagctcat tgtaccttca ggaccttagc gctgccgcta gcgagtgcat tgacccttcc
      661 gtggtgtttc cataccccct taacgactca agttcaccca aaagttgcgc ctcccaggac
      721 tctagtgctt ttagtcctag tagtgacagt ttactctcct ccactgagag ctcagactca
      781 gaagaggaac aagaagacga ggaggagatc gatgttgtct ccgtcgagaa gcgccaagcg
      841 cccggcaaac gatccgaatc agggagccct agtgcaggtg gtcacagtaa accgcctcat
      901 tcacctctcg tcctgaaacg atgccatgta agtacacatc agcacaacta tgcggcaccg
      961 ccaagtacca gaaaggatta tcctgctgca aaaagagtca aactggattc agtccgcgtt
     1021 cttaggcaaa tctctaataa tcgcaagtgt acctcacccc ggtctagtga cacagaggag
     1081 aatgtcaaga gaaggactca caatgtgttg gaacggcagc gaagaaacga gcttaaacgc
     1141 agcttcttcg cattgcgaga tcaaattcct gagcttgaga acaacgagaa ggcaccgaaa
     1201 gtcgtaatat tgaaaaaagc aacagcttac atattgtcag tccaggcaga ggagcaaaag
     1261 cttatctctg aagaggacct ccttcgaaaa agacgggaac aactcaagca caagcttgag
     1321 cagttgagga actcttgtgc atagtgacgt acggcggccg cctcgactag ggataacagg
     1381 gtaattgttt gaatgaggct tcagtacttt acagaatcgt tgcctgcaca tcttggaaac
     1441 acttgctggg attacttcga cttcttaacc caacagaagg ctcgagcaac cagaattcga
     1501 gcaattatct tgtttactaa aactgaatac cttgctatct ctttgataca tttttacaaa
     1561 gctgaattaa aatggtataa attaaatcac ttttttcaat tgacacgtac gcgtgatcta
     1621 attctactag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg
     1681 cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgat ccggtgccta
     1741 gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc
     1801 cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa
     1861 cgggtttgcc gccagaacac aggaccggtt gtagagcgct gccaccatga ccgagtacaa
     1921 gcccacggtg cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc
     1981 cgcgttcgcc gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg
     2041 ggtcaccgag ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg
     2101 ggtcgcggac gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg
     2161 ggcggtgttc gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc
     2221 gcagcaacag atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct
     2281 ggccaccgtc ggagtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct
     2341 ccccggagtg gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc
     2401 ccgcaacctc cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc
     2461 cgaaggaccg cgcacctggt gcatgacccg caagcccggt gcctgaacgc cgccatcgat
     2521 aaggagcgcc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     2581 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     2641 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     2701 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     2761 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     2821 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     2881 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     2941 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     3001 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaacgtcta ggccccccga
     3061 accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac aaccatgagc
     3121 agactggaca agagcaaagt cataaactct gctctggaat tactcaatgg agtcggtatc
     3181 gaaggcctga cgacaaggaa actcgctcaa aagctgggag ttgagcagcc taccctgtac
     3241 tggcacgtga agaacaagcg ggccctgctc gatgccctgc caatcgagat gctggacagg
     3301 catcataccc actcctgccc cctggaaggc gagtcatggc aagactttct gcggaacaac
     3361 gccaagtcat accgctgtgc tctcctctca catcgcgacg gggctaaagt gcatctcggc
     3421 acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc agctcgcgtt cctgtgtcag
     3481 caaggcttct ccctggagaa cgcactgtac gctctgtccg ccgtgggcca ctttacactg
     3541 ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag aggaaagaga gacacctacc
     3601 accgattcta tgcccccact tctgaaacaa gcaattgagc tgttcgaccg gcagggagcc
     3661 gaacctgcct tccttttcgg cctggaacta atcatatgtg gcctggagaa acagctaaag
     3721 tgcgaaagcg gcgggccgac cgacgccctt gacgattttg acttagacat gctcccagcc
     3781 gatgcccttg acgactttga ccttgatatg ctgcctgctg acgctcttga cgattttgac
     3841 cttgacatgc tccccgggta agctctagag ttaagtcgac aatcaacctc tggattacaa
     3901 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     3961 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     4021 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     4081 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     4141 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     4201 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     4261 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
     4321 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     4381 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
     4441 tcggatctcc ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca
     4501 gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc
     4561 caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga
     4621 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     4681 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     4741 agaccctttt agtcagtgtg gaaaatctct agcagggccc gtttaaaccc gctgatcagc
     4801 ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt
     4861 gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca
     4921 ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga
     4981 ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc
     5041 ggaaagaacc agccattaat gaaaagaacc agccattaat gaatcggcca acgcgcgggg
     5101 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     5161 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     5221 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     5281 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     5341 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     5401 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     5461 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     5521 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     5581 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     5641 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     5701 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     5761 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     5821 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     5881 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     5941 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     6001 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     6061 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     6121 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     6181 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     6241 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     6301 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     6361 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     6421 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     6481 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     6541 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     6601 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     6661 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     6721 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     6781 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     6841 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     6901 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     6961 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     7021 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
     7081 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
     7141 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
     7201 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
     7261 cgctgcttcg cgatgtacgg gccagatatt gacattgatt attgactagt tattaatagt
     7321 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     7381 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     7441 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     7501 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     7561 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     7621 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     7681 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     7741 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     7801 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     7861 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     7921 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     7981 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     8041 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     8101 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
     8161 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
     8221 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
     8281 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
     8341 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
     8401 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
     8461 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
     8521 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
     8581 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
     8641 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     8701 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     8761 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     8821 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     8881 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     8941 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     9001 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     9061 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     9121 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     9181 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     9241 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     9301 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     9361 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
     9421 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
     9481 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
     9541 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
     9601 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa ttaaggtacc
     9661 gggaggtggt ccctgcagtt acgccaatga taacccccgc cagaaaaatc ttagtagcct
     9721 tccctttttg ttttccgtgc cccaactcgg cggattgact cggccccttc cggaaacacc
     9781 cgaatcaact tctagtcaaa ttattgttca cgccgcaatg acccacccct ggcccgcgtc
     9841 tgtggaactg acccctggtg tacaggagag ttcgctgctg aaagtggtcc caaaggggta
     9901 ctagttttta agctcccaac tccccctccc ccagcgtctg gaggattcca caccctcgca
     9961 ccgcaggggc gaggaagtgg gcggagtccg gttttggcgc cagccgctga ggctgccaag
    10021 cagaaaagcc accgctgagg agactccggt cactgtcctc gccccgcctc ccccttccct
    10081 ccccttgggg accaccgggc gccacgccgc gaacggtaag tgccgcggtc gtcggcgcct
    10141 ccgccctccc cctagggccc caattcccag cgggcgcggc gcgcggcccc gcgcgcagct
    10201 cccggctccc tcccccttcg gatgtggctt gagctgtagg cgcggaggct agcctagatt
    10261 cgagtttact ccctatcagt gatagagaac gtatgaagag tttactccct atcagtgata
    10321 gagaacgtat gcagacttta ctccctatca gtgatagaga acgtataagg agtttactcc
    10381 ctatcagtga tagagaacgt atgaccagtt tactccctat cagtgataga gaacgtatct
    10441 acagtttact ccctatcagt gatagagaac gtatatccag tttactccct atcagtgata
    10501 gagaacgtat gtcgaggtag gcgtgtacgg tgggcgccta taaaagcaga gctcgtttag
    10561 tgaaccgtca gatcgcctgg agcaattcca caacactttt gtcttatact t
//
