LOCUS       pCMV-pLenti-ACTA2-L180V__(LLH669)        9106 bp    DNA     circular UNK 05-OCT-2025
DEFINITION  .
ACCESSION   <unknown
KEYWORDS    .
SOURCE      .
  ORGANISM  .
FEATURES             Location/Qualifiers
     rep_origin      1..589
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="ori"
     CDS             771..864
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="8.679593721144968"
                     /fragment="True"
                     /other="CDS"
                     /label="lacI"
     protein_bind    complement(877..898)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
                     /label="CAP binding site"
     promoter        913..943
                     /note="pLannotate"
                     /database="addgene"
                     /identity="96.875"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="lac promoter"
     protein_bind    complement(947..969)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="95.83333333333334"
                     /fragment="False"
                     /other="protein bind"
                     /label="lac operator"
     promoter        1012..1030
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="T3 promoter"
     promoter        1058..1284
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="RSV promoter"
     LTR             1285..1465
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
                     /label="5' LTR (truncated)"
     misc_feature    1512..1637
                     /note="pLannotate"
                     /database="addgene"
                     /identity="98.438"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
                     /label="HIV-1 Psi"
     CDS             1621..1984
                     /note="pLannotate"
                     /database="addgene"
                     /identity="97.802"
                     /match_length="24.266666666666666"
                     /fragment="True"
                     /other="CDS"
                     /label="HIV-1 gag"
     ncRNA           2079..2414
                     /note="pLannotate"
                     /database="infernal"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="ncRNA"
                     /label="RRE"
     misc_feature    2859..2976
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
                     /label="cPPT/CTS"
     enhancer        2990..3302
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="82.58575197889182"
                     /fragment="True"
                     /other="enhancer"
                     /label="CMV enhancer"
     promoter        3303..3506
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="CMV promoter"
     protein_bind    3623..3647
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
                     /label="attB1"
     CDS             3647..4777
                     /created_by="ben"
                     /modified_by="ben"
                     /label="ACTA2-L180V cDNA"
     misc_difference 4184..4186
                     /created_by="ben"
                     /label="L180V"
     CDS             4778..4780
                     /Color="#000000"
                     /created_by="ben"
                     /label
     protein_bind    complement(4779..4803)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein bind"
                     /label="attB2"
     CDS             4856..4897
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="V5 tag"
     misc_feature    4921..5509
                     /note="pLannotate"
                     /database="addgene"
                     /identity="99.831"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc feature"
                     /label="WPRE"
     promoter        5524..5840
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="SV40 promoter"
     CDS             5891..6610
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="EmGFP"
     misc_feature    6661..6680
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="17.094017094017094"
                     /fragment="True"
                     /other="misc feature"
                     /label="cPPT/CTS"
     LTR             6682..6915
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="LTR"
                     /label="3' LTR (Delta-U3)"
     polyA_signal    complement(6987..7118)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="98.507"
                     /match_length="97.8102189781022"
                     /fragment="False"
                     /other="polyA signal"
                     /label="SV40 poly(A) signal"
     promoter        7128..7287
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="81.63265306122449"
                     /fragment="True"
                     /other="promoter"
                     /label="SV40 promoter"
     promoter        complement(7304..7322)
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="T7 promoter"
     CDS             7330..7488
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="5.170731707317073"
                     /fragment="True"
                     /other="CDS"
                     /label="lacZ"
     rep_origin      7490..7945
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
                     /label="f1 ori"
     promoter        7971..8075
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
                     /label="AmpR promoter"
     CDS             8076..8936
                     /note="pLannotate"
                     /database="addgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
                     /label="AmpR"
ORIGIN      
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
     1021 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
     1081 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
     1141 cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
     1201 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
     1261 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
     1321 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     1381 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     1441 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
     1501 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
     1561 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
     1621 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
     1681 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
     1741 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1801 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1861 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1921 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1981 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     2041 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     2101 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     2161 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     2221 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     2281 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     2341 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     2401 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     2461 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     2521 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     2581 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     2641 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     2701 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     2761 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2821 attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa aggggggatt
     2881 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
     2941 gaattacaaa aacaaattac aaaaattcaa aattttatcg ataagcttgg gagttccgcg
     3001 ttacataact tacggtaaat ggcccgcctg gctgaccgcc caacgacccc cgcccattga
     3061 cgtcaataat gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat
     3121 gggtggagta tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa
     3181 gtacgccccc tattgacgtc aatgacggta aatggcccgc ctggcattat gcccagtaca
     3241 tgaccttatg ggactttcct acttggcagt acatctacgt attagtcatc gctattacca
     3301 tggtgatgcg gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat
     3361 ttccaagtct ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg
     3421 actttccaaa atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac
     3481 ggtgggaggt ctatataagc agagctcgtt tagtgaaccg tcagatcgcc tggagacgcc
     3541 atccacgctg ttttgacctc catagaagac accgactcta gaggatccac tagtccagtg
     3601 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gttggcatgt gtgaagaaga
     3661 ggacagcact gccttggtgt gtgacaatgg ctctgggctc tgtaaggccg gctttgctgg
     3721 ggacgatgct cccagggctg ttttcccatc cattgtggga cgtcccagac atcagggggt
     3781 gatggtggga atgggacaaa aagacagcta cgtgggtgac gaagcacaga gcaaaagagg
     3841 aatcctgacc ctgaagtacc cgatagaaca tggcatcatc accaactggg acgacatgga
     3901 aaagatctgg caccactctt tctacaatga gcttcgtgtt gcccctgaag agcatcccac
     3961 cctgctcacg gaggcacccc tgaaccccaa ggccaaccgg gagaaaatga ctcaaattat
     4021 gtttgagact ttcaatgtcc cagccatgta tgtggctatc caggcggtgc tgtctctcta
     4081 tgcctctgga cgcacaactg gcatcgtgct ggactctgga gatggtgtca cccacaatgt
     4141 ccccatctat gagggctatg ccttgcccca tgccatcatg cgtgtggatc tggctggccg
     4201 agatctcact gactacctca tgaagatcct gactgagcgt ggctattcct tcgttactac
     4261 tgctgagcgt gagattgtcc gggacatcaa ggagaaactg tgttatgtag ctctggactt
     4321 tgaaaatgag atggccactg ccgcatcctc atcctccctt gagaagagtt acgagttgcc
     4381 tgatgggcaa gtgatcacca tcggaaatga acgtttccgc tgcccagaga ccctgttcca
     4441 gccatccttc atcgggatgg agtctgctgg catccatgaa accacctaca acagcatcat
     4501 gaagtgtgat attgacatca ggaaggacct ctatgctaac aatgtcctat cagggggcac
     4561 cactatgtac cctggcattg ccgaccgaat gcagaaggag atcacggccc tagcacccag
     4621 caccatgaag atcaagatca ttgcccctcc ggagcgcaaa tactctgtct ggatcggtgg
     4681 ctccatcctg gcctctctgt ccaccttcca gcagatgtgg atcagcaaac aggaatacga
     4741 tgaagccggg ccttccattg tccaccgcaa atgcttctaa ccaactttct tgtacaaagt
     4801 ggttgatatc cagcacagtg gcggccgctc gagtctagag ggcccgcggt tcgaaggtaa
     4861 gcctatccct aaccctctcc tcggtctcga ttctacgcgt accggttagt aatgatcgac
     4921 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     4981 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     5041 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     5101 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     5161 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     5221 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     5281 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     5341 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     5401 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     5461 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggcgatggt
     5521 accggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca
     5581 attagtcagc aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa
     5641 gcatgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5701 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5761 cagaggccga ggccgcctct gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5821 gaggcctagg cttttgcaaa aagctcccgg gagcttgtat atccattttc ggatctgatc
     5881 agcacctagc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     5941 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     6001 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     6061 ctggcccacc ctcgtgacca ccttcaccta cggcgtgcag tgcttcgccc gctaccccga
     6121 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     6181 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     6241 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     6301 cctggggcac aagctggagt acaactacaa cagccacaag gtctatatca ccgccgacaa
     6361 gcagaagaac ggcatcaagg tgaacttcaa gacccgccac aacatcgagg acggcagcgt
     6421 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     6481 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     6541 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct
     6601 gtacaagtaa ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     6661 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     6721 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     6781 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     6841 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     6901 ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa
     6961 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     7021 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     7081 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     7141 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     7201 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     7261 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     7321 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     7381 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     7441 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     7501 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     7561 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     7621 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     7681 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     7741 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     7801 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     7861 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     7921 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     7981 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     8041 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     8101 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     8161 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     8221 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     8281 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     8341 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     8401 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     8461 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     8521 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     8581 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     8641 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     8701 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     8761 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     8821 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     8881 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     8941 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     9001 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     9061 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttc
//
