LOCUS       pLIX-Bahcc1-HA         15443 bp    DNA     circular SYN 13-JUL-2025
DEFINITION  1236.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15443)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15443
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    41..158
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     promoter        212..526
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     protein_bind    220..238
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    256..274
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    291..309
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    327..345
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    363..381
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    398..416
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     protein_bind    434..452
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
                     /note="color: #31849b"
     misc_feature    543..8309
                     /label=Bahcc1
                     /note="color: #ff8040; direction: RIGHT"
     misc_feature    6657..6676
                     /label=P1 primer
                     /note="color: #a6acb3"
     misc_feature    7393..8309
                     /label=BAH domain
                     /note="color: #a6acb3"
     CDS             8316..8342
                     /label=HA tag
                     /note="color: #008000; direction: RIGHT"
     CDS             8358..8375
                     /label=6His
                     /note="color: #008000; direction: RIGHT"
     promoter        8385..8895
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
                     /note="color: #ffffff; direction: RIGHT"
     misc_feature    8655..8674
                     /label=P2 primer
                     /note="color: #a6acb3"
     CDS             8905..9501
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /note="color: #ccffcc"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     CDS             9502..9555
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /note="color: #cc99b2
                     Cleavage site after base 9552"
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             join(9556..10173,10174..10179,10180..10215,10216..10218,
                     10219..10254,10255..10257,10258..10293,10294..10302)
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /note="This feature has 8 segments:
                        1:  9556 .. 10173 / #993366 / rTetR
                        2: 10174 .. 10179 / #cc99b2
                        3: 10180 .. 10215 / #993366 / VP16 minimal transcript...
                        4: 10216 .. 10218 / #cc99b2
                        5: 10219 .. 10254 / #993366 / VP16 minimal transcript...
                        6: 10255 .. 10257 / #cc99b2
                        7: 10258 .. 10293 / #993366 / VP16 minimal transcript...
                        8: 10294 .. 10302 / #cc99b2"
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(10760..10771)
                     /label=FactorXa site
                     /note="color: #008000; direction: LEFT"
     LTR             10976..11209
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    10976..11028
                     /label=delta U3
                     /note="color: #4682b4; direction: RIGHT"
     LTR             11029..11209
                     /label=HIV-1 5 LTR
                     /note="color: #ffa500; direction: RIGHT"
     polyA_signal    11281..11402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      11442..11577
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     misc_feature    11442..11519
                     /label=SV40 ORI
                     /note="color: #4682b4; direction: RIGHT"
     promoter        11577..11648
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: RIGHT"
     promoter        11579..11607
                     /label=Amp prom
                     /note="color: #4682b4; direction: RIGHT"
     CDS             join(11649..11717,11718..12509)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 11649 .. 11717 / #ccffcc / signal sequence
                        2: 11718 .. 12509 / #ccffcc
                     Cleavage site after base 11717"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     CDS             11847..12506
                     /label=AmpR
                     /note="color: #8fbc8f; direction: RIGHT"
     rep_origin      12658..13286
                     /direction=RIGHT
                     /label=ColE1 origin
                     /note="color: #4682b4"
     rep_origin      12680..13268
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     protein_bind    13556..13577
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     promoter        join(13592..13597,13598..13615,13616..13622)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This forward directional feature has 3 segments:
                        1: 13592 .. 13597 / #ffffff / -35
                        2: 13598 .. 13615 / #ffffff
                        3: 13616 .. 13622 / #ffffff / -10"
     promoter        13592..13621
                     /label=lac
                     /note="color: #4682b4; direction: RIGHT"
     misc_binding    13626..13648
                     /label=LacO
                     /note="color: #6495ed; direction: RIGHT"
     protein_bind    13630..13646
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     primer_bind     13654..13674
                     /label=M13-rev
                     /note="color: #008b8b; direction: RIGHT"
     primer_bind     13654..13670
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: RIGHT"
     promoter        13691..13709
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     13692..13711
                     /label=T3
                     /note="color: #008b8b; direction: RIGHT"
     promoter        13737..13963
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
                     /note="color: #ffffff; direction: RIGHT"
     LTR             13964..14144
                     /label=HIV-1 5 LTR
                     /note="color: #ffa500; direction: RIGHT"
     misc_feature    14191..14316
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    14255..14299
                     /label=HIV-1 psi pack
                     /note="color: #ffa500; direction: RIGHT"
     misc_feature    14809..15042
                     /label=RRE
                     /note="color: #4682b4; direction: RIGHT"
     CDS             15227..15271
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /note="color: #cc99b2"
                     /translation="KNEQELLELDKWASL"
ORIGIN
        1 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
       61 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
      121 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
      181 gagatccact ttggccgcga atcgatatgt cgagtttact ccctatcagt gatagagaac
      241 gtatgtcgag tttactccct atcagtgata gagaacgatg tcgagtttac tccctatcag
      301 tgatagagaa cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt
      361 actccctatc agtgatagag aacgtatgtc gagtttatcc ctatcagtga tagagaacgt
      421 atgtcgagtt tactccctat cagtgataga gaacgtatgt cgaggtaggc gtgtacggtg
      481 ggaggcctat ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gaattggcta
      541 gcatggcttc gcacacagcc agcagccgtc tgatgggaaa ccccccagcc tcctctttta
      601 tgggcagctt cctcaccagc agcctgggct cagcagcgtc ggcacacccc agtggcccca
      661 catcctctcc ctctgagccg gcctaccgag gatcccaccc tgccacctct cagatctggt
      721 tctcccactc ccatgaagct cctgcgtacc ccagattttc ggggagtctg gcatctacct
      781 tcctacccgt gagccacttg gatcaccatg gaaacagcaa tgttctctat gggcaacatc
      841 gtttctatgg aacccaaaaa gataacttct acctgcgcaa cctgccccca cagcctacca
      901 tactacccgc caaccacaac ttccccggag tgccccgagc cacccctgcc catcccatcg
      961 ggtcctgcag ccgggaccgc atcgaggctg cctcactgca gaagggtcct aaggagtttg
     1021 accgcttcct catgggtaaa gaggtgggca aagagaaagt tagcaaggga gcagagggca
     1081 gggagcggcc agctgtggag gaagacagtg gtaaagatcg acagaagctg gtgccgccca
     1141 tgcctgccga ggggccctgc aaggaggcag gccctgcacc ccgggggtcc tgtgagggcc
     1201 gccccaaaca cctcacctct tgcctgctca acaccaaggt actcaatgga gacatgggca
     1261 aggcctccct ggccagctgt gcaggaggca tgctgggcag gcctgggaca ggtgtggcag
     1321 cacccggacg ctgtgccaag gaggtggcag gccccgtgga gcctgggcca gccttcagtg
     1381 agtgcctgga gcggcggcag atgctgcatc atgctgtatc ctacacagta ccctctggcc
     1441 tgcctaccgg gccacccccg cccctcagca caggcccagc gggctctttc ccctgcctgc
     1501 agctgcatgc aggcccagat gggctctgcc ctctgcagga caaagtctcc cgggacctaa
     1561 aggccagcgg gcccaccttt gtgccttctg tgggacacct ggctgacaag agccgctctt
     1621 tccaagtagc agaggcctgt gccgtggcag gtgatggaaa ggaccggcac ctggatgcgg
     1681 ccatggccac cgaccatggt gcaccctatg gagtctccta cgcccaccta aaagctgagg
     1741 gcaagggcga gcggcgaccc gggggctttg aggcggccct ccacccccgg ctgaaaggcc
     1801 tggagtatct cagttcaggc cccgaggccc ccttccctgg acttcccaaa ggcggtctgg
     1861 acaaaagtgg ctactttgag ttgcccactt cacaggactg tgcccggtcc aatcaccaag
     1921 acccactggg tgggaaggcc acccaggcct gctgcacttt agacaaagtg gccaacaaag
     1981 aggcccctgc tggccctcca gtgggacaga aggtagccag gatccggcac cagcagcact
     2041 tggtggctcc tgaggtagaa tcgggaggca gcggggccga gacgaaacgc aagtccgtgg
     2101 agctggcttc tctgggttat agcggccacc acatgcctcc gtggggtgta cagacgggcc
     2161 acgacacctc catggccatc atcgaggaac gaaagggcag tgcctacctc gacccttttg
     2221 gaagtggctt acagcaggca gccctcctgt cccaggagct gcccactccg ccagatgagg
     2281 tctcggccat gaagaacctg ctgaaataca gcaaccaagc cctagtcgtg ggccagaagg
     2341 ctccgttcgt gggcctgggc agcctcaaag ccagctgcgt ccagcaggaa gccaagtttc
     2401 cagccactaa gggcccaggc ccagtggaaa gacccgactg tgcccgcagc agggagcatg
     2461 aggccccaca tggagacggg gaggtgaggc agccacccgt gggcatcgca gtggccttgg
     2521 ccaggcagaa ggacacggtc ggcaggcccg atacagccta caataccaac agtgggcggc
     2581 agggcagggc cgcccccacc ttcaaaggtg ctggaggacc acgtgccagc catgcgctag
     2641 acctggagag cgaagaggag aggtcacggg cgtgcgagga gcgcctgggt ctgcctggac
     2701 gtgagctgct gttacaagac aacaaagacc tcgtggaatt tgcccggatc cacccttcaa
     2761 gcagctgccc tggagacctg cccccccacc tcatgatgca aggcggggac ccagcccccc
     2821 acccccatcc tgcccatccc cactggctgc cccgcacccg cagtccctcc ttgtggatgg
     2881 ggggacattc ctatggtctc ggacaccccg ccctgcacca gaacctaccc cctggcttcc
     2941 ccgcatctgt gcccggctcc atgccctccg tgttccccct cccccaagat gcagccacgc
     3001 agttggtcat cttgccctcg gaacccacac cccacaccac ccctcacaca ctcgctgaag
     3061 ttatggacca ggcctccctg tggcccccca tgtatggggc ccggggccct gcctctcaca
     3121 tgcagcaccc gggccagctc cctgtgtatt ctcggtccca gctgcttcgg cagcaagagc
     3181 tctatgcact gcagcagcag cagcaacaac agcagcagca gcaacaacag cagcaacagc
     3241 agcagcaaca acaacagcag cagcagcaac agcagcagca gcaacatcgg gccacacagg
     3301 ccctggagct acagcgagtt gcccagttcc agcgcaagcc tgaggatcgc cacatggagc
     3361 tggaggaagc tgcccaggag aagaccccaa agtccaccca caagccagtt gccttaaccc
     3421 ccatggccaa gggcaccccc tcctccgcca ctgcaggcct ggtcaagcta tcaccctgct
     3481 gtcagtcgcc cactttgaag acccctgcga gttgccccac accaccacct cggcccagcg
     3541 ccccctgcac tttacccatc tgccctactg gcagcccagg gcctggctcc aaggtgccta
     3601 gtaccatgga caagagtgag gagggccagc gggctggaac caacctgacc acgctggaac
     3661 cagacctgac tccaggattg aaccccacag ctggcctgga tctctccctc ccttcagacg
     3721 tgcactcctc tgacctccaa gaccccaaaa ctatgcaaac cactacccca gggactcggc
     3781 ctgagccccc aaggacgttc ctccctgggg agccaccccc atgcagcccc aggaacttag
     3841 aagagcctgg gctgctctca agggccaggg atgccaccca ggaccttgcc aatctacccc
     3901 ctcctgttga gggaggactc ccaccaggga aggcagaaga ccccagccca ctcgaggggt
     3961 tacaagcact gaaatttgga gacctcctcg agggaggggg aactgaggct actggccaga
     4021 cgaattctac tcagggaggg atgcaaaatg agaggactgt agatcagggg gcaccacagc
     4081 cccctttggg ggctacccct caagcactag aacaggaagc agggagccca gctgccctgg
     4141 acaagcgtga aggtccacag aaggtccctg atgtggccca gctacaggag gaagaaaccc
     4201 agcttgagga gagcgggggg gactcggagg tggactgggg gactcccaac catagccacc
     4261 cacccaaagc gctgccaggc ttggatgcct tggtggctgc tactgtggac ctaggagacc
     4321 tgcctgacat cagcctgaca gaccctcaga ccccagcagc ctccgtgccc cttagcacgg
     4381 ctcctctgcc gcatagctca gggattcatg ggattgccct gctcagtgag ctggctgacc
     4441 tggagaccca gcggcagaag agtgagctgt ccatgcaaga agatgaggat gtgctggcct
     4501 tcaacctgca gcacctggcc acactggcca cagcctggtc cctggtggag gctgctaacc
     4561 tggacagccc agtcacttca ctgcaggccc cagctgctga cccagacaga ggccccaggc
     4621 tcacccccag aatgcaaatc ctgcaacgca aggacacctg ggcccctaaa acaaagcctg
     4681 tatgtcccct gaaggctgcc atcgaccggc tggacacaca ggaagtggag atgcgcatgc
     4741 agctagcaga gctgcagagg cgctacaagg agaagcaacg ggagctggct cgcctgcagc
     4801 gcaggcatga ccacgagaga gaggagagct cacggagccc tgcacggcgg gggcctggca
     4861 ggccaagaaa gcgcaaacac tcaagctcac tgcctgccct gcgtcccggg ggccagcttg
     4921 ccagaagtga cagcaagaaa gccaaggctg ttcgtgcaag cctaagtctg ctgtgtgctg
     4981 agctacgagg tgatgagccc ccaaggaagc gaagcaaact gggaaagagt ccttacactg
     5041 gcctgcagtc cgtctcctcg gagaaggtgc ggtgcaagaa gagctgtggc caggccgagt
     5101 tgccatcctc ggtggcccac aaggtggccc agctgaagcc aaaggtcaag agcaaggggc
     5161 tgcctgccgg cctcggtgcc ttccagcgca aagaggctgc cccaggtggg cgcatccaga
     5221 agaagctatc tagagccaag agcgtcacgg cgtcaggggc ggcacggcat ccacaccccg
     5281 atggggacag tggcagggag atgcacaaat tccaagccca acccgcagtg gctgtggcac
     5341 atgaggcagg cagcggctat gacagtgagg actgccaggc actcctgggg acagaggcag
     5401 ctcccagaga gcctgggctg gtgttgcacc cagggtctgg tgtggcagtg ctgggaccct
     5461 caccttcctc cgtggtcaag atggaagcca atcagaaggc caagaagaaa aaggagaggc
     5521 agggcttgct aggggcctgc cgcctgtcca gccccgaggg cgaggttaag atcaagagac
     5581 gaacagtaaa gaccaaggtg ggagccaagc tggagcgggc gccagggcgg cggcccccgg
     5641 gtgcaccagg caagaagaag gccaagggca aggtgaaaac tggcctgcgt acagagccag
     5701 gaaccgccac cagcagagac acccttttca gtcccacccg gacttttgcc tgccgagagg
     5761 agggcagcaa acttgccagt gagcgcctca agagagccac ccgcaagagc gccatgttgc
     5821 agccagtcct gaggcggaag aatggggcct tgtccatcgc tctgtcagcc cgcaacgcca
     5881 aggccatcct gggaaagagc aggaagctga cgaaggtgaa gagagaggct gtcagcaagc
     5941 agggccaggg gcgggcggtg agccggctgc tggagagctt tgctgtggag gatgactttg
     6001 agtttgacga ggatgacacc agcttctcgg atgaggaaga agaagaggaa gaagccggtg
     6061 tacagctcag cgcagagcag agcgccgccc tggcacggtc ctgcaccatc cacaaggaag
     6121 acctgcagga cggactgccc gtcctgatcc cgaaggagga cagcctgctg tatgcgggca
     6181 gtgtcaggac cctgcaaccc cccgacatct acagcatcgt cattgagggg gagagaggca
     6241 accgtcaacg tatctactcg ctggaacagc tgctgcagga ggcggttctt gatgtccagc
     6301 cacagtccag tcggtacctt ccacctggta cccgggtctg tgcctactgg agtcagaagt
     6361 cccggtgtct atatccaggc aatgtggttc gaggtgcttc aagcgatgaa gaagacctgg
     6421 actcagtgtt ggtagagttc gatgacggag acacaggcca catagctgtc tctaacatca
     6481 ggctgctgcc tccggacttc aagatccagt gtacagagcc ctcgccagcc ctgttggtgt
     6541 ccagcagctg ccggaggacc aaaaaagcag ccaatgaggg ccacccgccc agcgaagccc
     6601 ccacacccag cctgtcccct aaagtgccgg atggccctga aacttctaag acccctggga
     6661 agaaatctgg cagcaaagac aaagctggca aagtcgacct cctaacctca ggtgccaagt
     6721 cccccacggg ggcctcagac cacttcctag gccgcagagg cagccccctg ctgagctggt
     6781 cagcagtggc tcagaccaag cggaaagcgg tggctgcggc agcggcggcg gcaggtggca
     6841 aagggccggg ggtactacag aacctcttcc agctcaacgg aagcaccaag aagctgcggg
     6901 cccgggacac cctgtttccc atgcatagca tggctacccc cgtgtttggt aacagcttcc
     6961 gagctgactc cttcagcagc ctggcgagtt cctacacacc cttccttgga ggggctgggg
     7021 caggccttcc cggaggagcc cacaagctgc ttagggccaa gaaggctgaa cgggctgaag
     7081 cagaaaaggc cgggaggcgg cgggcaggcg gcgagtttct ggttaagctg gaccatgagg
     7141 gtgtgacctc ccccaaaaac aaaaactgca aggccctgct catgagcgac aaggacttcg
     7201 gacccaagct ggggcgacct cttagcaacc ccagttacgc acacccagcc ctcattggca
     7261 aggacaagaa gggtcgggcg cctgtgcatc cgctacccat ggggctggct ctgcgcaagt
     7321 acccactgcc ctgtgatagt gactgcccca gctcctactc cgatgaggac gaggatgggc
     7381 cggggctggc caccggtgtt ccctcccgat tcctcacccg cctatccatg tcgtcgtcct
     7441 cctctggctc gtccacgtct tcttcctcag gctccgtgtc cacgtccagc ctctgttcct
     7501 cagacaatga ggactcatct tatagctcag atgacgagga ccctgccctg ctgctgcaga
     7561 cctgtctcac ccgccctgtg cccgccctcc tggccccacc cgaagccctg cgctctaagg
     7621 gtagcagccc ccacgcgcac acccacgccc agcgctgctt cctgtccagg gctggggtgg
     7681 ctggtgcagg tgcaggggcc agccccagtg gtagcaaatc caagttcaag cgcaaggagg
     7741 ccctgagctt ctccaaagcc aaagagcttt ctcggaggca acggctgccc tccgtagaaa
     7801 accggccaaa gatctcagcc ttcctgcctg cccggcagct ctggaagtgg tcaggaaacc
     7861 ccacgcagag acgaggcatg aaggggaaag ccaggaaact attctacaag gccattgtcc
     7921 gaggcaagga gacactgcgc atcggggact gtgcggtctt cctttcggcc ggccggccca
     7981 acctgcccta cataggccgc atcgagagct tgtgggagtc ctggggcagc aacatggtgg
     8041 tgaaggtcaa atggttctac cacccagagg agaccaagct ggggaagcgg cagagtgacg
     8101 ggaagaacgc actgtaccag tcctgccacg aagatgagaa tgacgtgcag accatctccc
     8161 acaaatgtca ggtggtgggc cgagagcagt atgaacagat gatgcggggc cgcaagtacc
     8221 aggaccagca ggacctgtac tacttggcag gcacctatga tcctactact gggcgcctgg
     8281 tgacagctga cggcgtgccc gtcctgtgca ccggttatcc gtatgatgtg ccggattatg
     8341 cgtaaacgcg tccggtccac caccaccacc accactaagg atccggggtt ggggttgcgc
     8401 cttttccaag gcagccctgg gtttgcgcag ggacgcggct gctctgggcg tggttccggg
     8461 aaacgcagcg gcgccgaccc tgggtctcgc acattcttca cgtccgttcg cagcgtcacc
     8521 cggatcttcg ccgctaccct tgtgggcccc ccggcgacgc ttcctgctcc gcccctaagt
     8581 cgggaaggtt ccttgcggtt cgcggcgtgc cggacgtgac aaacggaagc cgcacgtctc
     8641 actagtaccc tcgcagacgg acagcgccag ggagcaatgg cagcgcgccg accgcgatgg
     8701 gctgtggcca atagcggctg ctcagcaggg cgcgccgaga gcagcggccg ggaaggggcg
     8761 gtgcgggagg cggggtgtgg ggcggtagtg tgggccctgt tcctgcccgc gcggtgttcc
     8821 gcattctgca agcctccgga gcgcacgtcg gcagtcggct ccctcgttga ccgaatcacc
     8881 gacctctctc cccagcaatt caccatgacc gagtacaagc ccacggtgcg cctcgccacc
     8941 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     9001 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     9061 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     9121 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     9181 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     9241 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     9301 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     9361 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     9421 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     9481 atgacccgca agcccggtgc cgaaggtaga ggttctctcc tcacttgtgg tgatgttgaa
     9541 gaaaaccctg gtccaatgtc tagactggac aagagcaaag tcataaacgg agctctggaa
     9601 ttactcaatg gtgtcggtat cgaaggcctg acgacaagga aactcgctca aaagctggga
     9661 gttgagcagc ctaccctgta ctggcacgtg aagaacaagc gggccctgct cgatgccctg
     9721 ccaatcgaga tgctggacag gcatcatacc cacttctgcc ccctggaagg cgagtcatgg
     9781 caagactttc tgcggaacaa cgccaagtca taccgctgtg ctctcctctc acatcgcgac
     9841 ggggctaaag tgcatctcgg cacccgccca acagagaaac agtacgaaac cctggaaaat
     9901 cagctcgcgt tcctgtgtca gcaaggcttc tccctggaga acgcactgta cgctctgtcc
     9961 gccgtgggcc actttacact gggctgcgta ttggaggaac aggagcatca agtagcaaaa
    10021 gaggaaagag agacacctac caccgattct atgcccccac ttctgagaca agcaattgag
    10081 ctgttcgacc ggcagggagc cgaacctgcc ttccttttcg gcctggaact aatcatatgt
    10141 ggcctggaga aacagctaaa gtgcgaaagc ggcgggccga ccgacgccct tgacgatttt
    10201 gacttagaca tgctcccagc cgatgccctt gacgactttg accttgatat gctgcctgct
    10261 gacgctcttg acgattttga ccttgacatg ctccccgggt aactaagtaa ggatcgatcc
    10321 aagatatcgt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
    10381 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
    10441 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
    10501 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
    10561 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
    10621 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
    10681 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
    10741 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
    10801 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
    10861 ttgggccgcc tccccgcctg tttcgcctcg gcgtccggac tagaggtacc tttaagacca
    10921 atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa
    10981 gggctaattc actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg
    11041 ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct
    11101 caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt
    11161 aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag tagtagttca
    11221 tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg
    11281 aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca
    11341 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
    11401 tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc
    11461 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
    11521 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggccttt
    11581 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
    11641 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
    11701 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
    11761 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
    11821 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
    11881 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
    11941 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
    12001 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
    12061 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
    12121 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
    12181 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
    12241 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
    12301 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
    12361 gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
    12421 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
    12481 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
    12541 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    12601 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    12661 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    12721 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    12781 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
    12841 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    12901 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    12961 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    13021 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    13081 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    13141 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    13201 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    13261 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
    13321 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    13381 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    13441 aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
    13501 gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
    13561 tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
    13621 tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
    13681 ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa gcttaatgta
    13741 gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc aacatgcctt
    13801 acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt acgatcgtgc
    13861 cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg aattgccgca
    13921 ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc tctggttaga
    13981 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
    14041 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
    14101 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg
    14161 gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa
    14221 gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc
    14281 ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga
    14341 tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat
    14401 atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca
    14461 tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa
    14521 gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag
    14581 ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc
    14641 accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga gggacaattg
    14701 gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac
    14761 caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt
    14821 ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt
    14881 acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat
    14941 tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag
    15001 aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc
    15061 tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct
    15121 ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac
    15181 aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga
    15241 attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct
    15301 gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt
    15361 tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat cgtttcagac
    15421 ccacctccca accccgaggg gac
//
