LOCUS       pAAV-eGFP-ct3-ALFA-c1                 5609 bp    DNA     circular SYN 10-JUN-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     promoter        5382..5486
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             join(5487..5609,1..738)
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             complement(2977..3693)
                     /note="pLannotate"
                     /label="EGFP"
                     /database="fpbase"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      909..1498
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.2"
                     /fragment="False"
                     /other="rep_origin"
     misc_feature    complement(2193..2778)
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="99.5"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      4900..5355
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="99.6"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(4114..4388)
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="98.2"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(4390..4638)
                     /note="pLannotate"
                     /label="CMV enhancer (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="81.9"
                     /fragment="True"
                     /other="enhancer"
     polyA_signal    2026..2157
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="99.2"
                     /match_length="97.8"
                     /fragment="False"
                     /other="polyA_signal"
     repeat_region   complement(1731..1864)
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="95.0"
                     /fragment="False"
                     /other="repeat_region"
     repeat_region   4699..4831
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="94.3"
                     /fragment="True"
                     /other="repeat_region"
     intron          complement(3978..4113)
                     /note="pLannotate"
                     /label="hybrid intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="59.6"
                     /fragment="True"
                     /other="intron"
     CDS             4826..4895
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="40.2"
                     /fragment="True"
                     /other="CDS"
     intron          complement(3759..3889)
                     /note="pLannotate"
                     /label="chimeric intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.9"
                     /fragment="True"
                     /other="intron"
     misc_feature    complement(2778..2802)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="23.1"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(3699..3761)
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="85.7"
                     /match_length="14.3"
                     /fragment="True"
                     /other="CDS"
     CDS             1681..1737
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.3"
                     /fragment="True"
                     /other="CDS"
     CDS             1935..2003
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="98.6"
                     /match_length="3.2"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
       61 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
      121 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
      181 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
      241 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
      301 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
      361 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
      421 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
      481 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
      541 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
      601 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
      661 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
      721 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
      781 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
      841 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
      901 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
      961 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     1021 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
     1081 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     1141 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     1201 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     1261 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     1321 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     1381 acgaggggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     1441 cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     1501 cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     1561 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg agtgagctga
     1621 taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg aagcggaaga
     1681 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctgcgc
     1741 gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac ctttggtcgc
     1801 ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat cactaggggt
     1861 tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg ctctaggaag
     1921 atccttatcg attttaccac atttgtagag gttttacttg ctttaaaaaa cctcccacat
     1981 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     2041 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     2101 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgctc
     2161 gaagcggccg cccgggtcga cggtatcgat gcgggaggcg gcccaaaggg agatccgact
     2221 cgtctgaggg cgaaggcgaa gacgcggaag aggccgcaga gccggcagca ggccgcggga
     2281 aggaaggtcc gctggattga gggccgaagg gacgtagcag aaggacgtcc cgcgcagaat
     2341 ccaggtggca acacaggcga gcagccaagg aaaggacgat gatttccccg acaacaccac
     2401 ggaattgtca gtgcccaaca gccgagcccc tgtccagcag cgggcaaggc aggcggcgat
     2461 gagttccgcc gtggcaatag ggagggggaa agcgaaagtc ccggaaagga gctgacaggt
     2521 ggtggcaatg ccccaaccag tgggggttgc gtcagcaaac acagtgcaca ccacgccacg
     2581 ttgcctgaca acgggccaca actcctcata aagagacagc aaccaggatt tatacaagga
     2641 ggagaaaatg aaagccatac gggaagcaat agcatgatac aaaggcatta aagcagcgta
     2701 tccacatagc gtaaaaggag caacatagtt aagaatacca gtcaatcttt cacaaatttt
     2761 gtaatccaga ggttgattat cgataagctt gatatcgaat tcttattctg taagtcttct
     2821 cctaagctcc tcctcgagtc tagaagaacc tcctgagcct ccttctgtca atcggcgtct
     2881 aagttcctcc tccaggcggg aagatccacc gctaccacct tcagtcaacc tgcgtcggag
     2941 ctcttcttcg agccgtgaag atcctccgct tccacccttg tacagctcgt ccatgccgag
     3001 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
     3061 gtctttgctc agggcggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
     3121 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
     3181 gttgtggcgg atcttgaagt tcaccttgat gccgttcttc tgcttgtcgg ccatgatata
     3241 gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
     3301 gaagtcgatg cccttcagct cgatgcggtt caccggggtg tcgccctcga acttcacctc
     3361 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
     3421 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcggctgaa
     3481 gcactgcacg ccgtaggtca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
     3541 ggtggtgcaa atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
     3601 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
     3661 cccggtgaac agctcctcgc ccttgctcac catggtggcg gatccaattc tttgccaaaa
     3721 tgatgagaca gcacaataac cagcacgttg cccaggagct gtaggaaaaa gaagaaggca
     3781 tgaacatggt tagcagaggc tctagagccg ccggtcacac gccagaagcc gaaccccgcc
     3841 ctgccccgtc ccccccgaag gcagccgtcc ccccgcggac agccccgagc cgctcccccc
     3901 gcacaaaggg ccctcccgga gcccctcaag gctttcacgc agccacagaa aagaaacaag
     3961 ccgtcattaa accaagcgct aattacagcc cggaggagaa gggccgtccc gcccgctcac
     4021 ctgtgggagt aacgcggtca gtcagagccg gggcgggcgg cgcgaggcgg cggcggagcg
     4081 gggcacgggg cgaaggcagc gcgcagcgac tcccgcccgc cgcgcgcttc gctttttata
     4141 gggccgccgc cgccgccgcc tcgccataaa aggaaacttt cggagcgcgc cgctctgatt
     4201 ggctgccgcc gcacctctcc gcctcgcccc gccccgcccc tcgccccgcc ccgccccgca
     4261 cggcgcgcgc cccccccccc ccgcccccat cgctgcacaa aataattaaa aaataaataa
     4321 atacaaaatt gggggtgggg agggggggga gatggggaga gtgaagcaga acgtggggct
     4381 cacctcgacc atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc
     4441 ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat
     4501 agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa
     4561 atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt
     4621 cattattgac gtcaatggta ctcttcctag agcatggcta cgtagataag tagcatggcg
     4681 ggttaatcat taactacaag gaacccctag tgatggagtt ggccactccc tctctgcgcg
     4741 ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg
     4801 cggcctcagt gagcgagcga gcgcgcagct ggcgtaatag cgaagaggcc cgcaccgatc
     4861 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     4921 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     4981 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5041 aagctctaaa tcaggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5101 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5161 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     5221 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     5281 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     5341 taacgcttac aatttaggtg gcacttttcg ggggaaatgt gcgcggaacc cctatttgtt
     5401 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5461 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5521 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5581 aagatgctga agatcagttg ggtgcacga
//
