LOCUS       p150_pAAV-eGFP-ct3-MYC-c3                 5584 bp    DNA     circular SYN 19-JUL-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             complement(2385..3101)
                     /note="pLannotate"
                     /label="EGFP"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    complement(1625..2213)
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      4312..4740
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    1461..1582
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        4793..4897
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             join(4898..5584,1..175)
                     /note="pLannotate"
                     /label="AmpR (fragment)"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.1"
                     /fragment="True"
                     /other="CDS"
     rep_origin      346..933
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        complement(3522..3800)
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="100.7"
                     /fragment="False"
                     /other="promoter"
     enhancer        complement(3802..4050)
                     /note="pLannotate"
                     /label="CMV enhancer (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="81.9"
                     /fragment="True"
                     /other="enhancer"
     repeat_region   complement(1166..1299)
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="95.0"
                     /fragment="False"
                     /other="repeat_region"
     repeat_region   4111..4243
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="94.3"
                     /fragment="True"
                     /other="repeat_region"
     intron          complement(3386..3521)
                     /note="pLannotate"
                     /label="hybrid intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="59.6"
                     /fragment="True"
                     /other="intron"
     CDS             4238..4307
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="40.2"
                     /fragment="True"
                     /other="CDS"
     intron          complement(3167..3297)
                     /note="pLannotate"
                     /label="chimeric intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.9"
                     /fragment="True"
                     /other="intron"
     misc_feature    complement(2213..2237)
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="23.1"
                     /fragment="True"
                     /other="misc_feature"
     CDS             complement(3107..3169)
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="85.7"
                     /match_length="14.3"
                     /fragment="True"
                     /other="CDS"
     CDS             1116..1172
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.3"
                     /fragment="True"
                     /other="CDS"
     CDS             1370..1438
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="98.6"
                     /match_length="3.2"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
       61 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
      121 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
      181 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
      241 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
      301 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
      361 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
      421 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
      481 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
      541 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
      601 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
      661 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
      721 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
      781 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
      841 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
      901 gtgatgctcg tcagggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
      961 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     1021 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     1081 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     1141 cccgcgcgtt ggccgattca ttaatgcagc tgcgcgctcg ctcgctcact gaggccgccc
     1201 gggcaaagcc cgggcgtcgg gcgacctttg gtcgcccggc ctcagtgagc gagcgagcgc
     1261 gcagagaggg agtggccaac tccatcacta ggggttcctt gtagttaatg attaacccgc
     1321 catgctactt atctacgtag ccatgctcta ggaagatcct tatcgatttt accacatttg
     1381 tagaggtttt acttgcttta aaaaacctcc cacatctccc cctgaacctg aaacataaaa
     1441 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
     1501 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     1561 ccaaactcat caatgtatct tatcatgtct gctcgaagcg gccgcccggg tcgacggtat
     1621 cgatgcgggg aggcggccca aagggagatc cgactcgtct gagggcgaag gcgaagacgc
     1681 ggaagaggcc gcagagccgg cagcaggccg cgggaaggaa ggtccgctgg attgagggcc
     1741 gaagggacgt agcagaagga cgtcccgcgc agaatccagg tggcaacaca ggcgagcagc
     1801 caaggaaagg acgatgattt ccccgacaac accacggaat tgtcagtgcc caacagccga
     1861 gcccctgtcc agcagcgggc aaggcaggcg gcgatgagtt ccgccgtggc aatagggagg
     1921 gggaaagcga aagtcccgga aaggagctga caggtggtgg caatgcccca accagtgggg
     1981 gttgcgtcag caaacacagt gcacaccacg ccacgttgcc tgacaacggg ccacaactcc
     2041 tcataaagag acagcaacca ggatttatac aaggaggaga aaatgaaagc catacgggaa
     2101 gcaatagcat gatacaaagg cattaaagca gcgtatccac atagcgtaaa aggagcaaca
     2161 tagttaagaa taccagtcaa tctttcacaa attttgtaat ccagaggttg attatcgata
     2221 agcttgatat cgaattctta caggtcctct tcggagataa gtttttgttc tgatccaccg
     2281 gaacccccca agtcttcttc gcttatcaat ttctgttcgc tacccccact gccgcccagg
     2341 tcttcctctg aaataagttt ctgctcgcta ccgcctgagc cgcccttgta cagctcgtcc
     2401 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
     2461 tcgttggggt ctttgctcag ggcggactgg gtgctcaggt agtggttgtc gggcagcagc
     2521 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
     2581 tcctcgatgt tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc
     2641 atgatataga cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg
     2701 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
     2761 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
     2821 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
     2881 cggctgaagc actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc
     2941 agcttgccgg tggtgcaaat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
     3001 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
     3061 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca tggtggcgga tccaattctt
     3121 tgccaaaatg atgagacagc acaataacca gcacgttgcc caggagctgt aggaaaaaga
     3181 agaaggcatg aacatggtta gcagaggctc tagagccgcc ggtcacacgc cagaagccga
     3241 accccgccct gccccgtccc ccccgaaggc agccgtcccc ccgcggacag ccccgagccg
     3301 ctccccccgc acaaagggcc ctcccggagc ccctcaaggc tttcacgcag ccacagaaaa
     3361 gaaacaagcc gtcattaaac caagcgctaa ttacagcccg gaggagaagg gccgtcccgc
     3421 ccgctcacct gtgggagtaa cgcggtcagt cagagccggg gcgggcggcg cgaggcggcg
     3481 gcggagcggg gcacggggcg aaggcagcgc gcagcgactc ccgcccgccg cgcgcttcgc
     3541 tttttatagg gccgccgccg ccgccgcctc gccataaaag gaaactttcg gagcgcgccg
     3601 ctctgattgg ctgccgccgc acctctccgc ctcgccccgc cccgcccctc gccccgcccc
     3661 gccccgcctg gcgcgcgccc cccccccccc ccgcccccat cgctgcacaa aataattaaa
     3721 aaataaataa atacaaaatt gggggtgggg aggggggggg gagatgggga gagtgaagca
     3781 gaacgtgggg ctcacctcga ccatggtaat agcgatgact aatacgtaga tgtactgcca
     3841 agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc atttaccgtc
     3901 attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg ccaagtgggc
     3961 agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg gcgttactat
     4021 gggaacatac gtcattattg acgtcaatgg tactcttcct agagcatggc tacgtagata
     4081 agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc
     4141 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     4201 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctggcgtaat agcgaagagg
     4261 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
     4321 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
     4381 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
     4441 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
     4501 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
     4561 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
     4621 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
     4681 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
     4741 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
     4801 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     4861 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     4921 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     4981 ggtgaaagta aaagatgctg aagatcagtt gggtgcactg agtgggttac atcgaactgg
     5041 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
     5101 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc
     5161 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
     5221 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
     5281 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
     5341 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
     5401 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
     5461 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
     5521 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
     5581 ttat
//
