LOCUS       pAAV-eGFP-ct3-NWS-c1                 5571 bp    DNA     circular SYN 10-JUN-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     misc_feature    3042..3630
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      complement(525..953)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(3673..3794)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(369..473)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(join(5080..5571,1..368))
                     /note="pLannotate"
                     /label="AmpR (fragment)"
                     /database="snapgene"
                     /identity="99.1"
                     /match_length="100.1"
                     /fragment="True"
                     /other="CDS"
     CDS             2162..2879
                     /note="pLannotate"
                     /label="EGFP (fragment)"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="99.7"
                     /fragment="True"
                     /other="CDS"
     rep_origin      complement(4322..4909)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        1465..1739
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="98.9"
                     /match_length="99.6"
                     /fragment="False"
                     /other="promoter"
     enhancer        1215..1463
                     /note="pLannotate"
                     /label="CMV enhancer (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="81.9"
                     /fragment="True"
                     /other="enhancer"
     repeat_region   3956..4089
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="95.0"
                     /fragment="False"
                     /other="repeat_region"
     repeat_region   complement(1028..1154)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.1"
                     /fragment="True"
                     /other="repeat_region"
     intron          1743..1878
                     /note="pLannotate"
                     /label="hybrid intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="59.6"
                     /fragment="True"
                     /other="intron"
     CDS             complement(958..1023)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="37.9"
                     /fragment="True"
                     /other="CDS"
     intron          1966..2096
                     /note="pLannotate"
                     /label="chimeric intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.9"
                     /fragment="True"
                     /other="intron"
     misc_feature    3018..3042
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="23.1"
                     /fragment="True"
                     /other="misc_feature"
     CDS             2094..2156
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="85.7"
                     /match_length="14.3"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(4083..4139)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.3"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3817..3885)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="98.6"
                     /match_length="3.2"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
       61 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
      121 gtcaatacgt gatataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
      181 cgttcttccg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
      241 acccactcgt gcacccaact gatcttcagc atctttactt tcaccagcgt ttctgggtga
      301 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgatacg gaaatgttga
      361 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      421 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgacatttc
      481 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
      541 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
      601 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
      661 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
      721 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
      781 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
      841 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
      901 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
      961 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     1021 ccaactacgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac
     1081 ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat
     1141 cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg
     1201 ctctaggaag agtaccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1261 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1321 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     1381 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     1441 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
     1501 tctccccccc tccccacccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     1561 cgatgggggc gggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
     1621 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
     1681 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggg
     1741 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
     1801 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     1861 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
     1921 aaagccttga ggggctccgg gagggccctt tgtgcggggg agcggctcgg ggctgtccgc
     1981 ggggggacgg ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga
     2041 ccggcggctc tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc
     2101 tgggcaacgt gctggttatt gtgctgtctc atcattttgg caaagaattg gatccgccac
     2161 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     2221 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     2281 cggcaagctg accctgaagt tcatttgcac caccggcaag ctgcccgtgc cctggcccac
     2341 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     2401 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccgaggag cgcaccatct
     2461 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2521 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2581 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     2641 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     2701 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2761 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     2821 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     2881 ggggatctgg cggttctaat tggtcacacc cccagttcga gaaaggtggt tccggcggta
     2941 gcaactggag ccatcctcaa ttcgaaaaag ggggaagcgg gggcagcaat tggagccacc
     3001 cacaatttga aaaataagaa ttcgatatca agcttatcga taatcaacct ctggattaca
     3061 aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat
     3121 acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct
     3181 ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac
     3241 gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca
     3301 cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca
     3361 tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg
     3421 tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga
     3481 ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt
     3541 cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga
     3601 gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacccggg cggccgcttc
     3661 gagcagacat gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa
     3721 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
     3781 gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga
     3841 tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaaaatc gataaggatc
     3901 ttcctagagc atggctacgt agataagtag catggcgggt taatcattaa ctacaaggaa
     3961 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     4021 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     4081 cgcagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     4141 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4201 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4261 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4321 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4381 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4441 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4501 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4561 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4621 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     4681 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     4741 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     4801 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     4861 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa ggatctcaag aagatccttt
     4921 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4981 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5041 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5101 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5161 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5221 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5281 gcgcagtagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5341 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5401 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5461 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5521 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg c
//
