LOCUS       plasmid                 5599 bp    DNA     circular SYN 10-AUG-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     CDS             2038..2754
                     /note="pLannotate"
                     /label="EGFP"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    2944..3532
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      complement(402..830)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     polyA_signal    complement(3575..3696)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(245..349)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(join(4983..5599,1..244))
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(4224..4812)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        1342..1617
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     enhancer        1092..1340
                     /note="pLannotate"
                     /label="CMV enhancer (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="81.9"
                     /fragment="True"
                     /other="enhancer"
     repeat_region   3858..3991
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="95.0"
                     /fragment="False"
                     /other="repeat_region"
     repeat_region   complement(899..1031)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="94.3"
                     /fragment="True"
                     /other="repeat_region"
     intron          1618..1753
                     /note="pLannotate"
                     /label="hybrid intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="59.6"
                     /fragment="True"
                     /other="intron"
     CDS             complement(835..904)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="40.2"
                     /fragment="True"
                     /other="CDS"
     intron          1842..1972
                     /note="pLannotate"
                     /label="chimeric intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.9"
                     /fragment="True"
                     /other="intron"
     misc_feature    2920..2944
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="23.1"
                     /fragment="True"
                     /other="misc_feature"
     CDS             1970..2032
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="85.7"
                     /match_length="14.3"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3985..4041)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.3"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3719..3787)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="98.6"
                     /match_length="3.2"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
       61 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
      121 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
      181 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
      241 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
      301 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
      361 gaaaagtgcc acctaaattg taagcgttaa tattttgtta aaattcgcgt taaatttttg
      421 ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt ataaatcaaa
      481 agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc cactattaaa
      541 gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg gcccactacg
      601 tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa
      661 ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg tggcgagaaa
      721 ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct
      781 gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt cccattcgcc
      841 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      901 gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt
      961 tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca actccatcac
     1021 taggggttcc ttgtagttaa tgattaaccc gccatgctac ttatctacgt agccatgctc
     1081 taggaagagt accattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     1141 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     1201 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     1261 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     1321 tagtcatcgc tattaccatg gtcgaggtga gccccacgtt ctgcttcact ctccccatct
     1381 ccccccctcc ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat
     1441 gggggcgggg gggggggggg gcgcgcgcca ggcggggcgg ggcggggcga ggggcggggc
     1501 ggggcgaggc ggagaggtgc ggcggcagcc aatcagagcg gcgcgctccg aaagtttcct
     1561 tttatggcga ggcggcggcg gcggcggccc tataaaaagc gaagcgcgcg gcgggcggga
     1621 gtcgctgcgc gctgccttcg ccccgtgccc cgctccgccg ccgcctcgcg ccgcccgccc
     1681 cggctctgac tgaccgcgtt actcccacag gtgagcgggc gggacggccc ttctcctccg
     1741 ggctgtaatt agcgcttggt ttaatgacgg cttgtttctt ttctgtggct gcgtgaaagc
     1801 cttgaggggc tccgggaggg ccctttgtgc ggggggagcg gctcggggct gtccgcgggg
     1861 ggacggctgc cttcgggggg gacggggcag ggcggggttc ggcttctggc gtgtgaccgg
     1921 cggctctaga gcctctgcta accatgttca tgccttcttc tttttcctac agctcctggg
     1981 caacgtgctg gttattgtgc tgtctcatca ttttggcaaa gaattggatc cgccaccatg
     2041 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
     2101 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
     2161 aagctgaccc tgaagttcat ttgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
     2221 gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag
     2281 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     2341 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     2401 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     2461 ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc
     2521 atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac
     2581 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     2641 ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     2701 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagggtggg
     2761 agcggggggt ccgaggatca agttgaccca agattgattg atggcaaggg cggatcaggt
     2821 gggtcagaag accaggtgga cccacgactc atagacggta aaggtggaag cggagggagc
     2881 gaggaccaag ttgatccacg acttattgac ggaaaataag aattcgatat caagcttatc
     2941 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3001 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3061 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     3121 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     3181 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     3241 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3301 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     3361 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3421 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3481 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     3541 cgtcgacccg ggcggccgct tcgagcagac atgataagat acattgatga gtttggacaa
     3601 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     3661 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     3721 atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     3781 tgtggtaaaa tcgataagga tcttcctaga gcatggctac gtagataagt agcatggcgg
     3841 gttaatcatt aactacaagg aacccctagt gatggagttg gccactccct ctctgcgcgc
     3901 tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc
     3961 ggcctcagtg agcgagcgag cgcgcagctg cattaatgaa tcggccaacg cgcggggaga
     4021 ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     4081 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     4141 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     4201 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     4261 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     4321 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     4381 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     4441 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     4501 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     4561 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     4621 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc
     4681 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     4741 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     4801 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     4861 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     4921 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     4981 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     5041 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     5101 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     5161 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     5221 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     5281 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     5341 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     5401 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     5461 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     5521 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     5581 tgctcttgcc cggcgtcaa
//
