LOCUS       pAAV-eGFP-ct3-SPOT-c1                 5599 bp    DNA     circular SYN 10-JUN-2022
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.0
FEATURES             Location/Qualifiers
     CDS             2043..2759
                     /note="pLannotate"
                     /label="EGFP"
                     /database="fpbase"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     misc_feature    2949..3537
                     /note="pLannotate"
                     /label="WPRE"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="misc_feature"
     rep_origin      complement(405..833)
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        1345..1622
                     /note="pLannotate"
                     /label="chicken β-actin promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     polyA_signal    complement(3580..3701)
                     /note="pLannotate"
                     /label="SV40 poly(A) signal"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="polyA_signal"
     promoter        complement(248..352)
                     /note="pLannotate"
                     /label="AmpR promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             complement(join(4986..5599,1..247))
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      complement(4228..4815)
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.7"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        1095..1343
                     /note="pLannotate"
                     /label="CMV enhancer (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="81.9"
                     /fragment="True"
                     /other="enhancer"
     repeat_region   3863..3995
                     /note="pLannotate"
                     /label="AAV2 ITR"
                     /database="snapgene"
                     /identity="99.3"
                     /match_length="95.0"
                     /fragment="False"
                     /other="repeat_region"
     repeat_region   complement(902..1034)
                     /note="pLannotate"
                     /label="AAV2 ITR (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="94.3"
                     /fragment="True"
                     /other="repeat_region"
     intron          1623..1758
                     /note="pLannotate"
                     /label="hybrid intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="59.6"
                     /fragment="True"
                     /other="intron"
     CDS             complement(838..907)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="40.2"
                     /fragment="True"
                     /other="CDS"
     intron          1847..1977
                     /note="pLannotate"
                     /label="chimeric intron (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="12.9"
                     /fragment="True"
                     /other="intron"
     misc_feature    2925..2949
                     /note="pLannotate"
                     /label="MCS (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="23.1"
                     /fragment="True"
                     /other="misc_feature"
     CDS             1975..2037
                     /note="pLannotate"
                     /label="HBB (fragment)"
                     /database="swissprot"
                     /identity="85.7"
                     /match_length="14.3"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3989..4045)
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="5.3"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(3724..3792)
                     /note="pLannotate"
                     /label="large T antigen (fragment)"
                     /database="snapgene"
                     /identity="98.6"
                     /match_length="3.2"
                     /fragment="True"
                     /other="CDS"
ORIGIN
        1 caatacggga taataccgcg ccacatagca gaactttaaa agtggtcatc attggaaaac
       61 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
      121 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
      181 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
      241 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
      301 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
      361 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
      421 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
      481 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
      541 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
      601 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
      661 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
      721 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
      781 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
      841 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      901 ccagctgcgc gctcgctcgc tcactgaggc cgcccgggca aagcccgggc gtcgggcgac
      961 ctttggtcgc ccggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat
     1021 cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg
     1081 ctctaggaag agtaccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
     1141 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
     1201 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
     1261 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
     1321 tattagtcat cgctattacc atggtcgagg tgagccccac gttctgcttc actctcccca
     1381 tctccccccc ctccccaccc ccaattttgt atttatttat tttttaatta ttttgtgcag
     1441 cgatgggggc gggggggggg ggggggcgcg cgccaggcgg ggcggggcgg ggcgaggggc
     1501 ggggcggggc gaggcggaga ggtgcggcgg cagccaatca gagcggcgcg ctccgaaagt
     1561 ttccttttat ggcgaggcgg cggcggcggc ggccctataa aaagcgaagc gcgcggcggg
     1621 cgggagtcgc tgcgcgctgc cttcgccccg tgccccgctc cgccgccgcc tcgcgccgcc
     1681 cgccccggct ctgactgacc gcgttactcc cacaggtgag cgggcgggac ggcccttctc
     1741 ctccgggctg taattagcgc ttggtttaat gacggcttgt ttcttttctg tggctgcgtg
     1801 aaagccttga ggggctccgg gagggccctt tgtgcggggg gagcggctcg gggctgtccg
     1861 cggggggacg gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg
     1921 accggcggct ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc
     1981 ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt ggatccgcca
     2041 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
     2101 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
     2161 acggcaagct gaccctgaag ttcatttgca ccaccggcaa gctgcccgtg ccctggccca
     2221 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
     2281 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
     2341 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
     2401 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
     2461 acaagctgga gtacaactac aacagccaca acgtctatat catggccgac aagcagaaga
     2521 acggcatcaa ggtgaacttc aagatccgcc acaacatcga ggacggcagc gtgcagctcg
     2581 ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg cccgacaacc
     2641 actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc gatcacatgg
     2701 tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag ctgtacaagg
     2761 gtggttccgg tggttctcca gacagagtaa gggccgtatc acactggagt agcggcggat
     2821 ctggtggttc acccgacagg gttagggcag tgtcacattg gtcaagcggg ggaagtgggg
     2881 gatctcctga ccgcgtacgc gcagtatccc attggtcatc ataagaattc gatatcaagc
     2941 ttatcgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     3001 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     3061 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     3121 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     3181 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     3241 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     3301 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaaatcatcg tcctttcctt
     3361 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     3421 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     3481 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
     3541 gataccgtcg acccgggcgg ccgcttcgag cagacatgat aagatacatt gatgagtttg
     3601 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     3661 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     3721 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct
     3781 acaaatgtgg taaaatcgat aaggatcttc ctagagcatg gctacgtaga taagtagcat
     3841 ggcgggttaa tcattaacta caaggaaccc ctagtgatgg agttggccac tcctctctgc
     3901 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     3961 gggcggcctc agtgagcgag cgagcgcgca gctgcattaa tgaatcggcc aacgcgcggg
     4021 gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     4081 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     4141 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     4201 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca
     4261 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     4321 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     4381 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     4441 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac ccccgttcag
     4501 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     4561 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     4621 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     4681 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     4741 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     4801 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     4861 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     4921 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     4981 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     5041 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     5101 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     5161 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     5221 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     5281 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     5341 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     5401 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     5461 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     5521 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     5581 agttgctctt gcccggcgt
//
