LOCUS       FUGW_mHif1a            12449 bp    DNA     circular SYN 08-MAR-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 12449)
  AUTHORS   Hart Lab
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..12449
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
                     /note="color: black; sequence: TGAAGCTCCGGTTTTGAACT"
     primer_bind     complement(1218..1235)
                     /label=APP_FUGW_R
                     /note="color: black; sequence: agtcgacctgcagcccaa; added: 
                     2022-08-25"
     primer_bind     complement(1236..1254)
                     /label=UbiCpro_R
                     /note="color: black; sequence: gtacccggggatcctctag; added: 
                     2019-06-19"
     regulatory      1260..1269
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             join(1266..1268,1269..1271,1272..1982)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 1266 .. 1268 / #05fd14
                        2: 1269 .. 1271 / #05fd14 / 1a
                        3: 1272 .. 1982 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1311..1332)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
                     /note="color: black; sequence: CGTCGCCGTCCAGCTCGACCAG"
     primer_bind     complement(1572..1591)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
                     /note="color: black; sequence: GTCTTGTAGTTGCCGTCGTC"
     primer_bind     1919..1940
                     /label=EGFP-C
                     /note="EGFP, forward primer"
                     /note="color: black; sequence: CATGGTCCTGCTGGAGTTCGTG"
     primer_bind     1963..2000
                     /label=mHif1a_Nfuse_F
                     /note="color: black; sequence: 
                     gcatggacgagctgtacaagatggagggcgccggcggc; added: 2022-12-20"
     primer_bind     complement(1964..1982)
                     /label=FUGW_Nfuse_R
                     /note="color: black; sequence: cttgtacagctcgtccatg; added: 
                     2022-12-20"
     gene            1983..4493
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /label=Hif1a
                     /note="hypoxia inducible factor 1, alpha subunit"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:15251"
                     /db_xref="MGI:MGI:106918"
     CDS             1983..4493
                     /codon_start=1
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /product="hypoxia-inducible factor 1-alpha isoform 2"
                     /label=Hif1a
                     /note="isoform 2 is encoded by transcript variant 2; 
                     hypoxia-inducible factor 1-alpha; ARNT-interacting protein"
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS25977.1"
                     /db_xref="GeneID:15251"
                     /db_xref="MGI:MGI:106918"
                     /protein_id="NP_034561.2"
                     /translation="MEGAGGENEKKKMSSERRKEKSRDAARSRRSKESEVFYELAHQLP
                     LPHNVSSHLDKASVMRLTISYLRVRKLLDAGGLDSEDEMKAQMDCFYLKALDGFVMVLT
                     DDGDMVYISDNVNKYMGLTQFELTGHSVFDFTHPCDHEEMREMLTHRNGPVRKGKELNT
                     QRSFFLRMKCTLTSRGRTMNIKSATWKVLHCTGHIHVYDTNSNQPQCGYKKPPMTCLVL
                     ICEPIPHPSNIEIPLDSKTFLSRHSLDMKFSYCDERITELMGYEPEELLGRSIYEYYHA
                     LDSDHLTKTHHDMFTKGQVTTGQYRMLAKRGGYVWVETQATVIYNTKNSQPQCIVCVNY
                     VVSGIIQHDLIFSLQQTESVLKPVESSDMKMTQLFTKVESEDTSCLFDKLKKEPDALTL
                     LAPAAGDTIISLDFGSDDTETEDQQLEDVPLYNDVMFPSSNEKLNINLAMSPLPSSETP
                     KPLRSSADPALNQEVALKLESSPESLGLSFTMPQIQDQPASPSDGSTRQSSPERLLQEN
                     VNTPNFSQPNSPSEYCFDVDSDMVNVFKLELVEKLFAEDTEAKNPFSTQDTDLDLEMLA
                     PYIPMDDDFQLRSFDQLSPLESNSPSPPSMSTVTGFQQTQLQKPTITATATTTATTDES
                     KTETKDNKEDIKILIASPSSTQVPQETTTAKASAYSGTHSRTASPDRAGKRVIEQTDKA
                     HPRSLNLSATLNQRNTVPEEELNPKTIASQNAQRKRKMEHDGSLFQAAGIGTLLQQPGD
                     CAPTMSLSWKRVKGFISSEQNGTEQKTIILIPSDLACRLLGQSMDESGLPQLTSYDCEV
                     NAPIQGSRNLLQGEELLRALDQVN"
     misc_feature    1983..3185
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61221.3);
                     Region: Interaction with TSGA10. 
                     /evidence=ECO:0000269|PubMed:16777103"
                     /note="color: #a6acb3"
     misc_feature    1983..2072
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61221.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="color: #a6acb3"
     exon            1983..2017
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     primer_bind     1983..1995
                     /label=mHif1a_CDS_F
                     /note="color: black; sequence: ATGGAGGGCGCCG; added: 
                     2022-12-14"
     primer_bind     complement(1992..2001)
                     /label=mHif1a_Nfuse_F
                     /note="color: black; sequence: 
                     gcatggacgagctgtacaagatggagggcgccggcggc; added: 2022-12-20"
     exon            2018..2208
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    2019..2231
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="basic helix-loop-helix-Per-ARNT-Sim (bHLH-PAS)
                     domain found in hypoxia-inducible factor 1-alpha (HIF1a) 
                     and similar proteins; Region: bHLH-PAS_HIF1a_PASD8; 
                     cd19727"
                     /note="color: #a6acb3"
                     /db_xref="CDD:381570"
     misc_feature    2034..2039
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /label=DNA binding site [nucleotide binding]
                     /note="DNA binding site [nucleotide binding]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:381570"
     misc_feature    2043..2072
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61221.3);
                     Region: DNA-binding. /evidence=ECO:0000269|PubMed:26245371"
                     /note="color: #a6acb3"
     misc_feature    2043..2051
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /label=DNA binding site [nucleotide binding]
                     /note="DNA binding site [nucleotide binding]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:381570"
     misc_feature    2058..2063
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /label=DNA binding site [nucleotide binding]
                     /note="DNA binding site [nucleotide binding]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:381570"
     misc_feature    2067..2072
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /label=DNA binding site [nucleotide binding]
                     /note="DNA binding site [nucleotide binding]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:381570"
     misc_feature    2076..2081
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="dimer interface [polypeptide binding]; other site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:381570"
     misc_feature    2085..2090
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="dimer interface [polypeptide binding]; other site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:381570"
     misc_feature    2097..2102
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="dimer interface [polypeptide binding]; other site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:381570"
     misc_feature    2109..2114
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="dimer interface [polypeptide binding]; other site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:381570"
     misc_feature    2145..2153
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="dimer interface [polypeptide binding]; other site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:381570"
     misc_feature    2145..2150
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /label=DNA binding site [nucleotide binding]
                     /note="DNA binding site [nucleotide binding]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:381570"
     misc_feature    2160..2165
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="dimer interface [polypeptide binding]; other site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:381570"
     misc_feature    2172..2174
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="dimer interface [polypeptide binding]; other site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:381570"
     misc_feature    2181..2186
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="dimer interface [polypeptide binding]; other site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:381570"
     misc_feature    2193..2195
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="dimer interface [polypeptide binding]; other site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:381570"
     exon            2209..2354
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     primer_bind     2228..2247
                     /label=mHif1aSeq_F
                     /note="color: black; sequence: tgagatgaaggcacagatgg; added:
                     2022-12-13"
     misc_feature    2259..2426
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="PAS domain; Region: PAS; smart00091"
                     /note="color: #a6acb3"
                     /db_xref="CDD:214512"
     exon            2355..2439
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     exon            2440..2552
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(2441..2458)
                     /label=mHif1a_T163_R
                     /note="color: black; sequence: TTCCCTTTTCTCACTGGG; added: 
                     2022-12-14"
     primer_bind     2459..2487
                     /label=mHif1a_T163E_F
                     /note="color: black; sequence: 
                     AGAACTAAACgagCAGCGGAGCTTTTTTC; added: 2022-12-14"
     primer_bind     2459..2481
                     /label=mHif1a_T163A_F
                     /note="color: black; sequence: AGAACTAAACgccCAGCGGAGCT; 
                     added: 2022-12-14"
     misc_feature    2469..2471
                     /label=T163
                     /note="color: #ff0000"
     misc_feature    2490..2555
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61221.3);
                     Region: Required for heterodimer formation with ARNT. 
                     /evidence=ECO:0000269|PubMed:26245371"
                     /note="color: #a6acb3"
     exon            2553..2755
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    2721..2723
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="Phosphoserine, by CK1. 
                     /evidence=ECO:0000250|UniProtKB:Q16665; propagated from 
                     UniProtKB/Swiss-Prot (Q61221.3); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    2742..2999
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="PAS fold; Region: PAS_3; pfam08447"
                     /note="color: #a6acb3"
                     /db_xref="CDD:430001"
     misc_feature    2745..2747
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /label=putative active site [active]
                     /note="putative active site [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238075"
     exon            2756..2862
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    2757..2759
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /label=putative active site [active]
                     /note="putative active site [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238075"
     misc_feature    2775..2777
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /label=putative active site [active]
                     /note="putative active site [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238075"
     misc_feature    2805..2807
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="heme pocket [chemical binding]; other site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238075"
     misc_feature    2814..2825
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /label=putative active site [active]
                     /note="putative active site [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238075"
     misc_feature    2817..2819
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="heme pocket [chemical binding]; other site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238075"
     misc_feature    2841..2843
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="heme pocket [chemical binding]; other site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238075"
     misc_feature    2850..2855
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="heme pocket [chemical binding]; other site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238075"
     exon            2863..3010
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    2901..2903
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /label=putative active site [active]
                     /note="putative active site [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238075"
     misc_feature    2916..2918
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /label=putative active site [active]
                     /note="putative active site [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238075"
     misc_feature    2937..2939
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="heme pocket [chemical binding]; other site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238075"
     misc_feature    2943..2945
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="heme pocket [chemical binding]; other site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238075"
     exon            3011..3231
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3120..3233
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61221.3);
                     Region: N-terminal VHL recognition site"
                     /note="color: #a6acb3"
     primer_bind     3170..3189
                     /label=mHif1a_mid_F
                     /note="color: black; sequence: tctcactctgctggctccag; added:
                     2022-12-08"
     primer_bind     complement(3170..3189)
                     /label=mHif1a_mid_R
                     /note="color: black; sequence: ctggagccagcagagtgaga; added:
                     2022-12-08"
     misc_feature    3183..3821
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61221.3);
                     Region: ODD"
                     /note="color: #a6acb3"
     misc_feature    3186..3188
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="4-hydroxyproline.
                     /evidence=ECO:0000250|UniProtKB:Q16665; propagated from 
                     UniProtKB/Swiss-Prot (Q61221.3); hydroxylation site"
                     /note="color: #a6acb3"
     exon            3232..3515
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3456..3515
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61221.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="color: #a6acb3"
     exon            3516..3680
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3612..3746
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61221.3);
                     Region: NTAD"
                     /note="color: #a6acb3"
     misc_feature    3615..3617
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:Q16665; propagated from 
                     UniProtKB/Swiss-Prot (Q61221.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    3672..3761
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="Hypoxia-inducible factor-1; Region: HIF-1;
                     pfam11413"
                     /note="color: #a6acb3"
                     /db_xref="CDD:431874"
     misc_feature    3672..3674
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="Phosphoserine, by GSK3-beta. 
                     /evidence=ECO:0000250|UniProtKB:Q16665; propagated from 
                     UniProtKB/Swiss-Prot (Q61221.3); phosphorylation site"
                     /note="color: #a6acb3"
     exon            3681..4105
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3684..3686
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="Phosphothreonine, by GSK3-beta. 
                     /evidence=ECO:0000250|UniProtKB:Q16665; propagated from 
                     UniProtKB/Swiss-Prot (Q61221.3); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    3687..3737
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61221.3);
                     Region: C-terminal VHL recognition site"
                     /note="color: #a6acb3"
     misc_feature    3711..3713
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="4-hydroxyproline.
                     /evidence=ECO:0000250|UniProtKB:Q16665; propagated from 
                     UniProtKB/Swiss-Prot (Q61221.3); hydroxylation site"
                     /note="color: #a6acb3"
     misc_feature    3747..4367
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61221.3);
                     Region: ID"
                     /note="color: #a6acb3"
     misc_feature    3747..3749
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="Phosphoserine, by PLK3. 
                     /evidence=ECO:0000250|UniProtKB:Q16665; propagated from 
                     UniProtKB/Swiss-Prot (Q61221.3); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    3759..4034
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61221.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="color: #a6acb3"
     misc_feature    3786..3788
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="Phosphoserine, by GSK3-beta. 
                     /evidence=ECO:0000250|UniProtKB:Q16665; propagated from 
                     UniProtKB/Swiss-Prot (Q61221.3); phosphorylation site"
                     /note="color: #a6acb3"
     primer_bind     complement(3963..3982)
                     /label=mHif1aSeq_R
                     /note="color: black; sequence: gccttagcagtggtcgtttc; added:
                     2022-12-13"
     misc_feature    3984..3986
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="Phosphoserine, by PLK3. 
                     /evidence=ECO:0000250|UniProtKB:Q16665; propagated from 
                     UniProtKB/Swiss-Prot (Q61221.3); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    4101..4184
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61221.3);
                     Region: Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="color: #a6acb3"
     exon            4106..4214
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    4137..4139
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:Q16665; propagated from 
                     UniProtKB/Swiss-Prot (Q61221.3); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    4164..4175
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61221.3);
                     Region: Nuclear localization signal. /evidence=ECO:0000255"
                     /note="color: #a6acb3"
     exon            4215..4341
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     exon            4342..4493
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    4368..4490
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="propagated from UniProtKB/Swiss-Prot (Q61221.3);
                     Region: CTAD"
                     /note="color: #a6acb3"
     misc_feature    4377..4487
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="HIF-1 alpha C terminal transactivation domain;
                     Region: HIF-1a_CTAD; pfam08778"
                     /note="color: #a6acb3"
                     /db_xref="CDD:430212"
     misc_feature    4419..4421
                     /gene="Hif1a"
                     /gene_synonym="bHLHe78; HIF-1-alpha; HIF1-alpha;
                     HIF1alpha; MOP1"
                     /note="(3S)-3-hydroxyasparagine. 
                     /evidence=ECO:0000250|UniProtKB:Q16665; propagated from 
                     UniProtKB/Swiss-Prot (Q61221.3); hydroxylation site"
                     /note="color: #a6acb3"
     primer_bind     complement(4441..4513)
                     /label=mHif1a_Nfuse_R
                     /note="color: black; sequence: 
                     aagcttgatatcgaattctatcagttaacttgatccaaagctctgagtaattcttcacc
                     ctgcagtaggtttc; added: 2022-12-20"
     primer_bind     complement(4471..4493)
                     /label=mHif1a_CDS_R
                     /note="color: black; sequence: TCAGTTAACTTGATCCAAAGCTC; 
                     added: 2022-12-14"
     primer_bind     4494..4517
                     /label=FUGW_Nfuse_F
                     /note="color: black; sequence: tagaattcgatatcaagcttatcg; 
                     added: 2022-12-20"
     misc_feature    4520..5108
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(4573..4593)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     CDS             complement(4991..5002)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 4990"
                     /translation="IEGR"
     primer_bind     complement(5111..5127)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(5112..5128)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
                     /note="color: black; sequence: TCGAGGTCGACGGTATC"
     LTR             5633..5813
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     complement(5839..5856)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
                     /note="color: black; sequence: TAGAAGGCACAGTCGAGG"
     polyA_signal    5845..6069
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      6115..6543
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(6202..6221)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="color: black; sequence: AGGGAAGAAAGCGAAAGGAG"
     primer_bind     6412..6433
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="color: black; sequence: GTGGACTCTTGTTCCAAACTGG"
     primer_bind     complement(6552..6572)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /note="color: black; sequence: ACCCTAACTGACACACATTCC"
     promoter        6557..6886
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      6737..6872
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     6799..6818
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     promoter        6934..6981
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             7000..7374
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7504..7625
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(7541..7560)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     7595..7614
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     primer_bind     complement(7674..7690)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(7674..7690)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     complement(7687..7709)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     protein_bind    7698..7714
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(join(7722..7728,7729..7746,7747..7752))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 7722 .. 7728 / #ffffff / -10
                        2: 7729 .. 7746 / #ffffff
                        3: 7747 .. 7752 / #ffffff / -35"
     protein_bind    7767..7788
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(7905..7922)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     rep_origin      complement(8076..8664)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(8156..8175)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     CDS             complement(join(8835..9626,9627..9695))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 8835 .. 9626 / #ccffcc
                        2: 9627 .. 9695 / #ccffcc / signal sequence
                     Cleavage site after base 9626"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9458..9477
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     promoter        complement(9696..9800)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(9875..9894)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /note="color: black; sequence: CGGCATCAGAGCAGATTGTA"
     enhancer        10066..10445
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        10447..10645
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     10600..10620
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
                     /note="color: black; sequence: CGCAAATGGGCGGTAGGCGTG"
     LTR             10663..10843
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    10890..11015
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    11508..11741
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    12268..12385
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     12422..12440
                     /label=UbiCpro_F
                     /note="color: black; sequence: ttaattaacccgtgtcggc; added: 
                     2019-06-19"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactctaga ggatccccgg gtaccggtcg
     1261 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     1321 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     1381 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     1441 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     1501 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     1561 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1621 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1681 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     1741 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     1801 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1861 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     1921 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1981 agatggaggg cgccggcggc gagaacgaga agaaaaagat gagttctgaa cgtcgaaaag
     2041 aaaagtctag agatgcagca agatctcggc gaagcaaaga gtctgaagtt ttttatgagc
     2101 ttgctcatca gttgccactt ccccacaatg tgagctcaca tcttgataaa gcttctgtta
     2161 tgaggctcac catcagttat ttacgtgtga gaaaacttct ggatgccggt ggtctagaca
     2221 gtgaagatga gatgaaggca cagatggact gtttttatct gaaagcccta gatggctttg
     2281 tgatggtgct aacagatgac ggcgacatgg tttacatttc tgataacgtg aacaaataca
     2341 tggggttaac tcagtttgaa ctaactggac acagtgtgtt tgattttact catccatgtg
     2401 accatgagga aatgagagaa atgcttacac acagaaatgg cccagtgaga aaagggaaag
     2461 aactaaacac acagcggagc ttttttctca gaatgaagtg caccctaaca agccggggga
     2521 ggacgatgaa catcaagtca gcaacgtgga aggtgcttca ctgcacgggc catattcatg
     2581 tctatgatac caacagtaac caacctcagt gtgggtacaa gaaaccaccc atgacgtgct
     2641 tggtgctgat ttgtgaaccc attcctcatc cgtcaaatat tgaaattcct ttagatagca
     2701 agacatttct cagtcgacac agcctcgata tgaaattttc ttactgtgat gaaagaatta
     2761 ctgagttgat gggttatgag ccggaagaac ttttgggccg ctcaatttat gaatattatc
     2821 atgctttgga ttctgatcat ctgaccaaaa ctcaccatga tatgtttact aaaggacaag
     2881 tcaccacagg acagtacagg atgcttgcca aaagaggtgg atatgtctgg gttgaaactc
     2941 aagcaactgt catatataat acgaagaact cccagccaca gtgcattgtg tgtgtgaatt
     3001 atgttgtaag tggtattatt cagcacgact tgattttctc ccttcaacaa acagaatctg
     3061 tgctcaaacc agttgaatct tcagatatga agatgactca gctgttcacc aaagttgaat
     3121 cagaggatac aagctgcctt tttgataagc ttaagaagga gcctgatgct ctcactctgc
     3181 tggctccagc tgccggcgac accatcatct ctctggattt tggcagcgat gacacagaaa
     3241 ctgaagatca acaacttgaa gatgttccat tatataatga tgtaatgttt ccctcttcta
     3301 atgaaaaatt aaatataaac ctggcaatgt ctcctttacc ttcatcggaa actccaaagc
     3361 cacttcgaag tagtgctgat cctgcactga atcaagaggt tgcattaaaa ttagaatcaa
     3421 gtccagagtc actgggactt tcttttacca tgccccagat tcaagatcag ccagcaagtc
     3481 cttctgatgg aagcactaga caaagttcac ctgagagact tcttcaggaa aacgtaaaca
     3541 ctcctaactt ttcccagcct aacagtccca gtgaatattg ctttgatgtg gatagcgata
     3601 tggtcaatgt attcaagttg gaactggtgg aaaaactgtt tgctgaagac acagaggcaa
     3661 agaatccatt ttcaactcag gacactgatt tagatttgga gatgctggct ccctatatcc
     3721 caatggatga tgatttccag ttacgttcct ttgatcagtt gtcaccatta gagagcaatt
     3781 ctccaagccc tccaagtatg agcacagtta ctgggttcca gcagacccag ttacagaaac
     3841 ctaccatcac tgccactgcc accacaactg ccaccactga tgaatcaaaa acagagacga
     3901 aggacaataa agaagatatt aaaatactga ttgcatctcc atcttctacc caagtacctc
     3961 aagaaacgac cactgctaag gcatcagcat acagtggcac tcacagtcgg acagcctcac
     4021 cagacagagc aggaaagaga gtcatagaac agacagacaa agctcatcca aggagcctta
     4081 acctgtctgc cactttgaat caaagaaata ctgttcctga ggaagaatta aacccaaaga
     4141 caatagcttc gcagaatgct cagaggaagc gaaaaatgga acatgatggc tccctttttc
     4201 aagcagcagg aattggaaca ttattgcagc aaccaggtga ctgtgcacct actatgtcac
     4261 tttcctggaa acgagtgaaa ggattcatat ctagtgaaca gaatggaacg gagcaaaaga
     4321 ctattatttt aataccctcc gatttagcat gcagactgct ggggcagtca atggatgaga
     4381 gtggattacc acagctgacc agttacgatt gtgaagttaa tgctcccata caaggcagca
     4441 gaaacctact gcagggtgaa gaattactca gagctttgga tcaagttaac tgatagaatt
     4501 cgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     4561 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     4621 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     4681 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     4741 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     4801 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     4861 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     4921 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     4981 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     5041 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     5101 ctccccgcat cgataccgtc gacctcgaga cctagaaaaa catggagcaa tcacaagtag
     5161 caatacagca gctaccaatg ctgattgtgc ctggctagaa gcacaagagg aggaggaggt
     5221 gggttttcca gtcacacctc aggtaccttt aagaccaatg acttacaagg cagctgtaga
     5281 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5341 acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg attggcagaa
     5401 ctacacacca gggccaggga tcagatatcc actgaccttt ggatggtgct acaagctagt
     5461 accagttgag caagagaagg tagaagaagc caatgaagga gagaacaccc gcttgttaca
     5521 ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtattagagt ggaggtttga
     5581 cagccgccta gcatttcatc acatggcccg agagctgcat ccggactgta ctgggtctct
     5641 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     5701 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     5761 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagggcccg
     5821 tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct gttgtttgcc
     5881 cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa
     5941 atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg
     6001 ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg gatgcggtgg
     6061 gctctatggc ttctgaggcg gaaagaacca gctggggctc tagggggtat ccccacgcgc
     6121 cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac
     6181 ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg
     6241 ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt
     6301 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
     6361 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
     6421 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
     6481 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
     6541 attaattctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     6601 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6661 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6721 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6781 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctctgcct ctgagctatt
     6841 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tcccgggagc
     6901 ttgtatatcc attttcggat ctgatcagca cgtgttgaca attaatcatc ggcatagtat
     6961 atcggcatag tataatacga caaggtgagg aactaaacca tggccaagtt gaccagtgcc
     7021 gttccggtgc tcaccgcgcg cgacgtcgcc ggagcggtcg agttctggac cgaccggctc
     7081 gggttctccc gggacttcgt ggaggacgac ttcgccggtg tggtccggga cgacgtgacc
     7141 ctgttcatca gcgcggtcca ggaccaggtg gtgccggaca acaccctggc ctgggtgtgg
     7201 gtgcgcggcc tggacgagct gtacgccgag tggtcggagg tcgtgtccac gaacttccgg
     7261 gacgcctccg ggccggccat gaccgagatc ggcgagcagc cgtgggggcg ggagttcgcc
     7321 ctgcgcgacc cggccggcaa ctgcgtgcac ttcgtggccg aggagcagga ctgacacgtg
     7381 ctacgagatt tcgattccac cgccgccttc tatgaaaggt tgggcttcgg aatcgttttc
     7441 cgggacgccg gctggatgat cctccagcgc ggggatctca tgctggagtt cttcgcccac
     7501 cccaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     7561 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     7621 tcttatcatg tctgtatacc gtcgacctct agctagagct tggcgtaatc atggtcatag
     7681 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     7741 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     7801 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
     7861 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
     7921 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     7981 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     8041 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     8101 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     8161 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     8221 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     8281 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     8341 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     8401 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     8461 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
     8521 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     8581 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     8641 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     8701 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
     8761 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
     8821 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
     8881 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
     8941 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
     9001 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
     9061 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
     9121 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
     9181 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
     9241 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
     9301 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
     9361 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
     9421 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
     9481 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
     9541 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
     9601 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
     9661 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
     9721 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
     9781 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
     9841 ggagatctcc cgatccccta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     9901 taagccagta tctgctccct gcttgtgtgt tggaggtcgc tgagtagtgc gcgagcaaaa
     9961 tttaagctac aacaaggcaa ggcttgaccg acaattgcat gaagaatctg cttagggtta
    10021 ggcgttttgc gctgcttcgc gatgtacggg ccagatatac gcgttgacat tgattattga
    10081 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
    10141 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
    10201 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
    10261 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
    10321 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
    10381 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
    10441 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
    10501 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
    10561 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
    10621 tacggtggga ggtctatata agcagcgcgt tttgcctgta ctgggtctct ctggttagac
    10681 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
    10741 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
    10801 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
    10861 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
    10921 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
    10981 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
    11041 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
    11101 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
    11161 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
    11221 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
    11281 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
    11341 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
    11401 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
    11461 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
    11521 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
    11581 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
    11641 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
    11701 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
    11761 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
    11821 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
    11881 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
    11941 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
    12001 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
    12061 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
    12121 cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga aggtggagag
    12181 agagacagag acagatccat tcgattagtg aacggatcgg cactgcgtgc gccaattctg
    12241 cagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
    12301 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
    12361 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg
    12421 gttaattaac ccgtgtcggc tccagatct
//
