LOCUS       FUGW_mPkm2             11534 bp    DNA     circular SYN 10-MAR-2023
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 11534)
  AUTHORS   Hart Lab
  TITLE     Direct Submission
  JOURNAL   Exported Jul 14, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..11534
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     promoter        1..1212
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     1049..1068
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
                     /note="color: black; sequence: TGAAGCTCCGGTTTTGAACT"
     primer_bind     complement(1218..1235)
                     /label=APP_FUGW_R
                     /note="color: black; sequence: agtcgacctgcagcccaa; added: 
                     2022-08-25"
     primer_bind     complement(1236..1254)
                     /label=UbiCpro_R
                     /note="color: black; sequence: gtacccggggatcctctag; added: 
                     2019-06-19"
     regulatory      1260..1269
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /note="color: #a6acb3"
                     /regulatory_class="other"
     CDS             join(1266..1268,1269..1271,1272..1982)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /note="This feature has 3 segments:
                        1: 1266 .. 1268 / #05fd14
                        2: 1269 .. 1271 / #05fd14 / 1a
                        3: 1272 .. 1982 / #05fd14"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1311..1332)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
                     /note="color: black; sequence: CGTCGCCGTCCAGCTCGACCAG"
     primer_bind     complement(1572..1591)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
                     /note="color: black; sequence: GTCTTGTAGTTGCCGTCGTC"
     primer_bind     1919..1940
                     /label=EGFP-C
                     /note="EGFP, forward primer"
                     /note="color: black; sequence: CATGGTCCTGCTGGAGTTCGTG"
     primer_bind     1963..2001
                     /label=mPkm2_Nfuse_F
                     /note="color: black; sequence: 
                     gcatggacgagctgtacaagatgccgaagccacacagtg; added: 2022-12-20"
     primer_bind     complement(1964..1982)
                     /label=FUGW_Nfuse_R
                     /note="color: black; sequence: cttgtacagctcgtccatg; added: 
                     2022-12-20"
     CDS             1983..3578
                     /codon_start=1
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /product="pyruvate kinase PKM isoform M2"
                     /EC_number="2.7.1.40; 2.7.11.1; 2.7.10.2"
                     /label=Pkm
                     /note="isoform M2 is encoded by transcript variant 2;
                     pyruvate kinase isozymes M1/M2; pyruvate kinase muscle 
                     isozyme; pyruvate kinase 3; tyrosine-protein kinase PKM2; 
                     threonine-protein kinase PKM2; pyruvate kinase PKM"
                     /note="color: #993366"
                     /db_xref="CCDS:CCDS40659.1"
                     /db_xref="GeneID:18746"
                     /db_xref="MGI:MGI:97591"
                     /protein_id="NP_035229.2"
                     /translation="MPKPHSEAGTAFIQTQQLHAAMADTFLEHMCRLDIDSAPITARNT
                     GIICTIGPASRSVEMLKEMIKSGMNVARLNFSHGTHEYHAETIKNVREATESFASDPIL
                     YRPVAVALDTKGPEIRTGLIKGSGTAEVELKKGATLKITLDNAYMEKCDENILWLDYKN
                     ICKVVEVGSKIYVDDGLISLQVKEKGADFLVTEVENGGSLGSKKGVNLPGAAVDLPAVS
                     EKDIQDLKFGVEQDVDMVFASFIRKAADVHEVRKVLGEKGKNIKIISKIENHEGVRRFD
                     EILEASDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNRAGKPVICATQMLESMIKKPRP
                     TRAEGSDVANAVLDGADCIMLSGETAKGDYPLEAVRMQHLIAREAEAAIYHLQLFEELR
                     RLAPITSDPTEAAAVGAVEASFKCCSGAIIVLTKSGRSAHQVARYRPRAPIIAVTRNPQ
                     TARQAHLYRGIFPVLCKDAVLNAWAEDVDLRVNLAMDVGKARGFFKKGDVVIVLTGWRP
                     GSGFTNTMRVVPVP"
     gene            1983..3578
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=Pkm
                     /note="pyruvate kinase, muscle"
                     /note="color: #a6acb3; direction: RIGHT"
                     /db_xref="GeneID:18746"
                     /db_xref="MGI:MGI:97591"
     exon            1983..2136
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     primer_bind     1983..2000
                     /label=mPkm_CDS_F
                     /note="color: black; sequence: atgccgaagccacacagt; added: 
                     2022-12-14"
     misc_feature    1989..1991
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="N6,N6,N6-trimethyllysine. 
                     /evidence=ECO:0000250|UniProtKB:P14618; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); methylation site"
                     /note="color: #a6acb3"
     misc_feature    2091..2093
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P14618; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    2103..2105
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:P14618; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    2106..3572
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="Pyruvate kinase (PK): Large allosteric enzyme that 
                     regulates glycolysis through binding of the substrate, 
                     phosphoenolpyruvate, and one or more allosteric effectors. 
                     Like other allosteric enzymes, PK has a high substrate 
                     affinity R state and a low...; Region: Pyruvate_Kinase; 
                     cd00288"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    2112..2117
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     exon            2137..2228
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    2166..2168
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0007744|PubMed:23806337; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2199..2201
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=active site
                     /note="active site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    2205..2207
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=active site
                     /note="active site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     exon            2229..2360
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    2247..2249
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P14618; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2271..2273
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P11980; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    2280..2282
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P11980; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    2295..2297
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0007744|PubMed:18034455; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    2301..2303
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    2307..2309
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    2319..2321
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=active site
                     /note="active site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    2331..2342
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     exon            2361..2547
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    2361..2363
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P14618; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    2424..2426
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0007744|PubMed:18034455; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    2478..2480
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0007744|PubMed:23806337; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2505..2507
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="Phosphotyrosine.
                     /evidence=ECO:0000250|UniProtKB:P14618; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); phosphorylation site"
                     /note="color: #a6acb3"
     exon            2548..2818
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    2565..2567
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="Phosphothreonine.
                     /evidence=ECO:0000250|UniProtKB:P14618; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); phosphorylation site"
                     /note="color: #a6acb3"
     misc_feature    2607..2612
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    2628..2630
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    2637..2651
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    2694..2696
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    2712..2714
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=active site
                     /note="active site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    2718..2720
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     primer_bind     2734..2753
                     /label=mPkmSeq_F
                     /note="color: black; sequence: tgcatgaagtcaggaaggtg"
     misc_feature    2739..2741
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    2772..2774
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    2778..2780
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0000250|UniProtKB:P14618; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2790..2792
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="Transition state stabilizer. 
                     /evidence=ECO:0000250|UniProtKB:P00549; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); other site"
                     /note="color: #a6acb3"
     misc_feature    2790..2792
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0007744|PubMed:23806337; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2790..2792
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=active site
                     /note="active site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    2796..2798
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=active site
                     /note="active site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     exon            2819..2969
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    2868..2870
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=active site
                     /note="active site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    2880..2882
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    2895..2897
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P14618; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2901..3575
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="propagated from UniProtKB/Swiss-Prot (P52480.4);
                     Region: Interaction with POU5F1. 
                     /evidence=ECO:0000250|UniProtKB:P14618"
                     /note="color: #a6acb3"
     misc_feature    2934..2939
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     primer_bind     complement(2936..2953)
                     /label=mPkm2_T328_R
                     /note="color: black; sequence: ACAGGCTTCCCAGCTCGG; added: 
                     2022-12-14"
     misc_feature    2946..2948
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="N6-acetyllysine, alternate. 
                     /evidence=ECO:0007744|PubMed:23806337; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    2949..2951
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     primer_bind     2954..2984
                     /label=mPkm2_T328E_F
                     /note="color: black; sequence: 
                     CATCTGTGCCgagCAGATGCTGGAGAGCATG; added: 2022-12-14"
     primer_bind     2954..2978
                     /label=mPkm2_T328A_F
                     /note="color: black; sequence: CATCTGTGCCgccCAGATGCTGGAG; 
                     added: 2022-12-14"
     misc_feature    2964..2966
                     /label=T328
                     /note="color: #ff0000"
     misc_feature    2964..2966
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=active site
                     /note="active site"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     exon            2970..3122
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3045..3047
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    3051..3053
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     exon            3123..3289
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3138..3140
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    3144..3149
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    3147..3281
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="propagated from UniProtKB/Swiss-Prot (P52480.4);
                     Region: Intersubunit contact"
                     /note="color: #a6acb3"
     misc_feature    3189..3191
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="4-hydroxyproline.
                     /evidence=ECO:0000250|UniProtKB:P14618; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); hydroxylation site"
                     /note="color: #a6acb3"
     misc_feature    3204..3206
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="4-hydroxyproline.
                     /evidence=ECO:0000250|UniProtKB:P14618; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); hydroxylation site"
                     /note="color: #a6acb3"
     misc_feature    3279..3281
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0000250|UniProtKB:P14618; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); acetylation site"
                     /note="color: #a6acb3"
     misc_feature    3279..3281
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="Crucial for phosphotyrosine binding. 
                     /evidence=ECO:0000250|UniProtKB:P14618; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); other site"
                     /note="color: #a6acb3"
     exon            3290..3471
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     misc_feature    3309..3311
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    3315..3317
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    3321..3323
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    3354..3356
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    3363..3368
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    3372..3374
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    3378..3386
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /label=domain interfaces [active]
                     /note="domain interfaces [active]"
                     /note="color: #a6acb3"
                     /db_xref="CDD:238178"
     misc_feature    3405..3407
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="N6-acetyllysine.
                     /evidence=ECO:0007744|PubMed:23806337; propagated from 
                     UniProtKB/Swiss-Prot (P52480.4); acetylation site"
                     /note="color: #a6acb3"
     exon            3472..3578
                     /gene="Pkm"
                     /gene_synonym="Pk-2; Pk-3; Pk3; Pkm2"
                     /note="color: #708090"
                     /inference="alignment:same species:2.1.0"
     primer_bind     complement(3558..3578)
                     /label=mPkm_CDS_R
                     /note="color: black; sequence: tcaaggtacaggcactacacg; 
                     added: 2022-12-14"
     primer_bind     complement(3559..3598)
                     /label=mPkm2_Nfuse_R
                     /note="color: black; sequence: 
                     aagcttgatatcgaattctatcaaggtacaggcactacac; added: 
                     2022-12-20"
     primer_bind     3579..3602
                     /label=FUGW_Nfuse_F
                     /note="color: black; sequence: tagaattcgatatcaagcttatcg; 
                     added: 2022-12-20"
     misc_feature    3605..4193
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
                     /note="color: #ffffff"
     primer_bind     complement(3658..3678)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
                     /note="color: black; sequence: CATAGCGTAAAAGGAGCAACA"
     CDS             complement(4076..4087)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /note="color: #cc99b2
                     Cleavage site after base 4075"
                     /translation="IEGR"
     primer_bind     complement(4196..4212)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(4197..4213)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
                     /note="color: black; sequence: TCGAGGTCGACGGTATC"
     LTR             4718..4898
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     complement(4924..4941)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
                     /note="color: black; sequence: TAGAAGGCACAGTCGAGG"
     polyA_signal    4930..5154
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
                     /note="color: #a6acb3"
     rep_origin      5200..5628
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
                     /note="color: #ffff00"
     primer_bind     complement(5287..5306)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
                     /note="color: black; sequence: AGGGAAGAAAGCGAAAGGAG"
     primer_bind     5497..5518
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
                     /note="color: black; sequence: GTGGACTCTTGTTCCAAACTGG"
     primer_bind     complement(5637..5657)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
                     /note="color: black; sequence: ACCCTAACTGACACACATTCC"
     promoter        5642..5971
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
                     /note="color: #ffffff; direction: RIGHT"
     rep_origin      5822..5957
                     /label=SV40 ori
                     /note="SV40 origin of replication"
                     /note="color: #ffff00"
     primer_bind     5884..5903
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
                     /note="color: black; sequence: TATTTATGCAGAGGCCGAGG"
     promoter        6019..6066
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
                     /note="color: #ffffff; direction: RIGHT"
     CDS             6085..6459
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /note="color: #ccffcc"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    6589..6710
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
                     /note="color: #a6acb3"
     primer_bind     complement(6626..6645)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
                     /note="color: black; sequence: GAAATTTGTGATGCTATTGC"
     primer_bind     6680..6699
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
                     /note="color: black; sequence: GTGGTTTGTCCAAACTCATC"
     primer_bind     complement(6759..6775)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
                     /note="color: #a020f0; direction: LEFT"
     primer_bind     complement(6759..6775)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
                     /note="color: black; sequence: CAGGAAACAGCTATGAC"
     primer_bind     complement(6772..6794)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
                     /note="color: black; sequence: AGCGGATAACAATTTCACACAGG"
     protein_bind    6783..6799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
                     /note="color: #31849b"
     promoter        complement(join(6807..6813,6814..6831,6832..6837))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
                     /note="This reverse directional feature has 3 segments:
                        1: 6807 .. 6813 / #ffffff / -10
                        2: 6814 .. 6831 / #ffffff
                        3: 6832 .. 6837 / #ffffff / -35"
     protein_bind    6852..6873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
                     /note="color: #31849b"
     primer_bind     complement(6990..7007)
                     /label=L4440
                     /note="L4440 vector, forward primer"
                     /note="color: black; sequence: AGCGAGTCAGTGAGCGAG"
     rep_origin      complement(7161..7749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     primer_bind     complement(7241..7260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
                     /note="color: black; sequence: GGGAAACGCCTGGTATCTTT"
     CDS             complement(join(7920..8711,8712..8780))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="This feature has 2 segments:
                        1: 7920 .. 8711 / #ccffcc
                        2: 8712 .. 8780 / #ccffcc / signal sequence
                     Cleavage site after base 8711"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8543..8562
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
                     /note="color: black; sequence: ATAATACCGCGCCACATAGC"
     promoter        complement(8781..8885)
                     /gene="bla"
                     /label=AmpR promoter
                     /note="color: #ffffff; direction: LEFT"
     primer_bind     complement(8960..8979)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
                     /note="color: black; sequence: CGGCATCAGAGCAGATTGTA"
     enhancer        9151..9530
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
                     /note="color: #ffffff"
     promoter        9532..9730
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
                     /note="color: #ffffff; direction: RIGHT"
     primer_bind     9685..9705
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
                     /note="color: black; sequence: CGCAAATGGGCGGTAGGCGTG"
     LTR             9748..9928
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
                     /note="color: #ffe4c4"
     misc_feature    9975..10100
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
                     /note="color: #ffe4c4"
     misc_feature    10593..10826
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
                     /note="color: #ffe4c4"
     misc_feature    11353..11470
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
                     /note="color: #ffe4c4"
     primer_bind     11507..11525
                     /label=UbiCpro_F
                     /note="color: black; sequence: ttaattaacccgtgtcggc; added: 
                     2019-06-19"
ORIGIN
        1 ggcctccgcg ccgggttttg gcgcctcccg cgggcgcccc cctcctcacg gcgagcgctg
       61 ccacgtcaga cgaagggcgc agcgagcgtc ctgatccttc cgcccggacg ctcaggacag
      121 cggcccgctg ctcataagac tcggccttag aaccccagta tcagcagaag gacattttag
      181 gacgggactt gggtgactct agggcactgg ttttctttcc agagagcgga acaggcgagg
      241 aaaagtagtc ccttctcggc gattctgcgg agggatctcc gtggggcggt gaacgccgat
      301 gattatataa ggacgcgccg ggtgtggcac agctagttcc gtcgcagccg ggatttgggt
      361 cgcggttctt gtttgtggat cgctgtgatc gtcacttggt gagtagcggg ctgctgggct
      421 ggccggggct ttcgtggccg ccgggccgct cggtgggacg gaagcgtgtg gagagaccgc
      481 caagggctgt agtctgggtc cgcgagcaag gttgccctga actgggggtt ggggggagcg
      541 cagcaaaatg gcggctgttc ccgagtcttg aatggaagac gcttgtgagg cgggctgtga
      601 ggtcgttgaa acaaggtggg gggcatggtg ggcggcaaga acccaaggtc ttgaggcctt
      661 cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc tggggaccct
      721 gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg gggcggcagt
      781 tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc tcgtcgtgtc
      841 gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc ggtaggtgtg
      901 cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc tctcctgaat
      961 cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt tctttggtcg
     1021 gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat gcgctcgggg
     1081 ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc atttgggtca
     1141 atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc gtttttggct
     1201 tttttgttag acgaagcttg ggctgcaggt cgactctaga ggatccccgg gtaccggtcg
     1261 ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc
     1321 tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca
     1381 cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc
     1441 ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac cccgaccaca
     1501 tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca
     1561 tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca
     1621 ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg
     1681 ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc gacaagcaga
     1741 agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc agcgtgcagc
     1801 tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca
     1861 accactacct gagcacccag tccgccctga gcaaagaccc caacgagaag cgcgatcaca
     1921 tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca
     1981 agatgccgaa gccacacagt gaagcaggga ctgccttcat tcagacccag cagctccatg
     2041 cagccatggc tgacaccttc ctggaacaca tgtgccgcct ggacattgac tctgccccca
     2101 tcacggcccg caacactggc atcatttgta ccattgggcc tgcttcccga tctgtggaga
     2161 tgctgaagga gatgattaag tctggaatga atgtggctcg gctgaatttc tctcatggaa
     2221 cccatgagta ccatgcagag accatcaaga atgtccgtga agccacagaa agctttgcat
     2281 ctgatcccat tctctaccgt cctgttgcgg tggctctgga tacaaaggga cctgagatcc
     2341 ggactggact catcaagggc agcggcaccg ctgaggtgga gctgaagaag ggagccactc
     2401 tgaagatcac cctggacaac gcttacatgg agaagtgtga cgagaacatc ctgtggctgg
     2461 actacaagaa catctgcaag gtggtggagg tgggcagcaa gatctacgtg gacgatgggc
     2521 tcatctcact gcaggtgaag gagaaaggcg ctgacttcct ggtgacggag gtggagaatg
     2581 gtggctcctt gggcagcaag aagggcgtga acctgccggg cgctgctgtg gatctccccg
     2641 ctgtgtcgga aaaggacatc caggacctga agtttggggt ggagcaggat gtggacatgg
     2701 tgtttgcatc tttcatccgc aaggcagccg acgtgcatga agtcaggaag gtgctgggag
     2761 agaagggcaa gaacatcaag atcatcagca aaatcgagaa ccatgaaggc gtccgcaggt
     2821 ttgatgagat cttggaggcc agtgatggga tcatggtggc tcgtggtgac ctgggcattg
     2881 agattcctgc agagaaggtc ttcctggctc agaagatgat gatcgggcga tgcaaccgag
     2941 ctgggaagcc tgtcatctgt gccacacaga tgctggagag catgatcaag aagccacgcc
     3001 ccacccgtgc tgaaggcagt gatgtggcca atgcagtcct ggatggagca gactgcatca
     3061 tgctgtctgg agaaacagcc aagggggact accctctgga ggctgttcgc atgcagcacc
     3121 tgattgcccg agaggcagag gctgccatct accacttgca gctattcgag gaactccgcc
     3181 gcctggcgcc cattaccagc gaccccacag aagctgccgc cgtgggtgcc gtggaggcct
     3241 ccttcaagtg ctgcagtggg gccattatcg tgctcaccaa gtctggcagg agtgctcacc
     3301 aagtggccag gtaccgccct cgggctccta tcattgccgt gactcgaaat ccccagactg
     3361 ctcgccaggc ccatctgtac cgtggcatct tccctgtgct gtgtaaggat gccgtgctga
     3421 atgcctgggc tgaggatgtc gaccttcgtg taaacttggc catggatgtt ggcaaggccc
     3481 gaggcttctt caagaaggga gatgtggtca ttgtgctgac cgggtggcgc cctggctctg
     3541 gattcaccaa caccatgcgt gtagtgcctg taccttgata gaattcgata tcaagcttat
     3601 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     3661 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     3721 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     3781 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     3841 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     3901 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3961 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     4021 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     4081 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     4141 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     4201 ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata cagcagctac
     4261 caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt ttccagtcac
     4321 acctcaggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     4381 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag atatccttga
     4441 tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca caccagggcc
     4501 agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag ttgagcaaga
     4561 gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg tgagcctgca
     4621 tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc gcctagcatt
     4681 tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt tagaccagat
     4741 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     4801 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     4861 cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa acccgctgat
     4921 cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
     4981 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
     5041 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
     5101 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg
     5161 aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat
     5221 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     5281 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     5341 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     5401 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     5461 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     5521 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     5581 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa
     5641 tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     5701 catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag
     5761 aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc
     5821 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     5881 ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg
     5941 aggctttttt ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt
     6001 cggatctgat cagcacgtgt tgacaattaa tcatcggcat agtatatcgg catagtataa
     6061 tacgacaagg tgaggaacta aaccatggcc aagttgacca gtgccgttcc ggtgctcacc
     6121 gcgcgcgacg tcgccggagc ggtcgagttc tggaccgacc ggctcgggtt ctcccgggac
     6181 ttcgtggagg acgacttcgc cggtgtggtc cgggacgacg tgaccctgtt catcagcgcg
     6241 gtccaggacc aggtggtgcc ggacaacacc ctggcctggg tgtgggtgcg cggcctggac
     6301 gagctgtacg ccgagtggtc ggaggtcgtg tccacgaact tccgggacgc ctccgggccg
     6361 gccatgaccg agatcggcga gcagccgtgg gggcgggagt tcgccctgcg cgacccggcc
     6421 ggcaactgcg tgcacttcgt ggccgaggag caggactgac acgtgctacg agatttcgat
     6481 tccaccgccg ccttctatga aaggttgggc ttcggaatcg ttttccggga cgccggctgg
     6541 atgatcctcc agcgcgggga tctcatgctg gagttcttcg cccaccccaa cttgtttatt
     6601 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     6661 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgt
     6721 ataccgtcga cctctagcta gagcttggcg taatcatggt catagctgtt tcctgtgtga
     6781 aattgttatc cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc
     6841 tggggtgcct aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc
     6901 cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc
     6961 ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt
     7021 cggctgcggc gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca
     7081 ggggataacg caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa
     7141 aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat
     7201 cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc
     7261 cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc
     7321 gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt
     7381 tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac
     7441 cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg
     7501 ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca
     7561 gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc
     7621 gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa
     7681 accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa
     7741 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
     7801 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
     7861 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
     7921 taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata
     7981 gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc
     8041 agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac
     8101 cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag
     8161 tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac
     8221 gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc
     8281 agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg
     8341 gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc
     8401 atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct
     8461 gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc
     8521 tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc
     8581 atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc
     8641 agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc
     8701 gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca
     8761 cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt
     8821 tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt
     8881 ccgcgcacat ttccccgaaa agtgccacct gacgtcgacg gatcgggaga tctcccgatc
     8941 ccctatggtg cactctcagt acaatctgct ctgatgccgc atagttaagc cagtatctgc
     9001 tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag caaaatttaa gctacaacaa
     9061 ggcaaggctt gaccgacaat tgcatgaaga atctgcttag ggttaggcgt tttgcgctgc
     9121 ttcgcgatgt acgggccaga tatacgcgtt gacattgatt attgactagt tattaatagt
     9181 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
     9241 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
     9301 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
     9361 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
     9421 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
     9481 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtgatgcggt
     9541 tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt ccaagtctcc
     9601 accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat
     9661 gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct
     9721 atataagcag cgcgttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     9781 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg
     9841 cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc
     9901 ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag
     9961 ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag
    10021 gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc tagaaggaga
    10081 gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga tgggaaaaaa
    10141 ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta tgggcaagca
    10201 gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa ggctgtagac
    10261 aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt agatcattat
    10321 ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa gacaccaagg
    10381 aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg
    10441 ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag tgaattatat
    10501 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
    10561 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
    10621 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
    10681 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
    10741 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
    10801 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
    10861 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
    10921 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
    10981 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
    11041 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
    11101 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
    11161 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
    11221 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
    11281 tccattcgat tagtgaacgg atcggcactg cgtgcgccaa ttctgcagac aaatggcagt
    11341 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
    11401 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
    11461 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggttaa ttaacccgtg
    11521 tcggctccag atct
//
