LOCUS       pFS267_pFS550_26        8247 bp    DNA     circular SYN 27-NOV-2024
DEFINITION  pFS267_pFS550_26.
ACCESSION   pFS267_pFS550_26
VERSION     pFS267_pFS550_26
KEYWORDS    .
SOURCE      
  ORGANISM  .
            .
COMMENT     Annotated with pLannotate v1.2.2
FEATURES             Location/Qualifiers
     rep_origin      5633..6061
                     /note="pLannotate"
                     /label="f1 ori"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     enhancer        complement(3865..4244)
                     /note="pLannotate"
                     /label="CMV enhancer"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="enhancer"
     promoter        complement(3661..3864)
                     /note="pLannotate"
                     /label="CMV promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     CDS             6405..7196
                     /note="pLannotate"
                     /label="neo"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     terminator      2516..2713
                     /note="pLannotate"
                     /label="TEF terminator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="terminator"
     gene            1138..2713
                     /note="pLannotate"
                     /label="hphMX6"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="gene"
     CDS             complement(2939..3559)
                     /note="pLannotate"
                     /label="tetR"
                     /database="swissprot"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             7217..8077
                     /note="pLannotate"
                     /label="AmpR"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     CDS             4637..5350
                     /note="pLannotate"
                     /label="10B"
                     /database="fpbase"
                     /identity="98.3"
                     /match_length="100.0"
                     /fragment="False"
                     /other="CDS"
     rep_origin      1..589
                     /note="pLannotate"
                     /label="ori"
                     /database="snapgene"
                     /identity="99.8"
                     /match_length="100.0"
                     /fragment="False"
                     /other="rep_origin"
     promoter        913..943
                     /note="pLannotate"
                     /label="lac promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    877..898
                     /note="pLannotate"
                     /label="CAP binding site"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     promoter        complement(5451..5469)
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     promoter        2908..2926
                     /note="pLannotate"
                     /label="T7 promoter"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="promoter"
     protein_bind    951..967
                     /note="pLannotate"
                     /label="lac operator"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="100.0"
                     /fragment="False"
                     /other="protein_bind"
     CDS             5472..5629
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="90.8"
                     /fragment="True"
                     /other="CDS"
     CDS             complement(2745..2900)
                     /note="pLannotate"
                     /label="lacZα (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="89.7"
                     /fragment="True"
                     /other="CDS"
     CDS             6074..6373
                     /note="pLannotate"
                     /label="tnpA (fragment)"
                     /database="swissprot"
                     /identity="99.0"
                     /match_length="21.0"
                     /fragment="True"
                     /other="CDS"
     CDS             772..864
                     /note="pLannotate"
                     /label="lacI (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="8.6"
                     /fragment="True"
                     /other="CDS"
     promoter        4552..4573
                     /note="pLannotate"
                     /label="H1-2O2 promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="23.2"
                     /fragment="True"
                     /other="promoter"
     promoter        7200..7216
                     /note="pLannotate"
                     /label="AmpR promoter (fragment)"
                     /database="snapgene"
                     /identity="100.0"
                     /match_length="26.6"
                     /fragment="True"
                     /other="promoter"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg gtaccgagct
     1021 cggatccact agtaacggcc gccagtgtgc tggaattcgc ccttcggatc cccgggttaa
     1081 ttaaggcgcg ccagatctgt ttagcttgcc tcgtccccgc cgggtcaccc ggccagcgac
     1141 atggaggccc agaataccct ccttgacagt cttgacgtgc gcagctcagg ggcatgatgt
     1201 gactgtcgcc cgtacattta gcccatacat ccccatgtat aatcatttgc atccatacat
     1261 tttgatggcc gcacggcgcg aagcaaaaat tacggctcct cgctgcggac ctgcgagcag
     1321 ggaaacgctc ccctcacaga cgcgttgaat tgtccccacg ccgcgcccct gtagagaaat
     1381 ataaaaggtt aggatttgcc actgaggttc ttctttcata tacttccttt taaaatcttg
     1441 ctaggataca gttctcacat cacatccgaa cataaacaac catgggtaaa aagcctgaac
     1501 tcaccgcgac gtctgtcgag aagtttctga tcgaaaagtt cgacagcgtc tccgacctga
     1561 tgcagctctc ggagggcgaa gaatctcgtg ctttcagctt cgatgtagga gggcgtggat
     1621 atgtcctgcg ggtaaatagc tgcgccgatg gtttctacaa agatcgttat gtttatcggc
     1681 actttgcatc ggccgcgctc ccgattccgg aagtgcttga cattggggaa ttcagcgaga
     1741 gcctgaccta ttgcatctcc cgccgtgcac agggtgtcac gttgcaagac ctgcctgaaa
     1801 ccgaactgcc cgctgttctg cagccggtcg cggaggccat ggatgcgatc gctgcggccg
     1861 atcttagcca gacgagcggg ttcggcccat tcggaccgca aggaatcggt caatacacta
     1921 catggcgtga tttcatatgc gcgattgctg atccccatgt gtatcactgg caaactgtga
     1981 tggacgacac cgtcagtgcg tccgtcgcgc aggctctcga tgagctgatg ctttgggccg
     2041 aggactgccc cgaagtccgg cacctcatgc acgcggattt cggctccaac aatgtcctga
     2101 cggacaatgg ccgcataaca gcggtcattg actggagcgg ggcgatgttc ggggattccc
     2161 aatacgaggt cgccaacatc ttcttctgga ggccgtggtt ggcttgtatg gagcagcaga
     2221 cgcgctactt cgagcggagg catccggagc ttgcaggatc gccgcggctc cgggcgtata
     2281 tgctccgcat tggtcttgac caactctatc agagcttggt tgacggcaat ttcgatgatg
     2341 cagcttgggc gcagggtcga tgcgacgcaa tcgtccgatc cggagccggg actgtcgggc
     2401 gtacacaaat cgcccgcaga agcgcggccg tctggaccga tggctgtgta gaagtactcg
     2461 ccgatagtgg aaaccgacgc cccagcactc gtccgagggc aaaggaataa tcagtactga
     2521 caataaaaag attcttgttt tcaagaactt gtcatttgta tagttttttt atattgtagt
     2581 tgttctattt taatcaaatg ttagcgtgat ttatattttt tttcgcctcg acatcatctg
     2641 cccagatgcg aagttaagtg cgcagaaagt aatatcatgc gtcaatcgta tgtgaatgct
     2701 ggtcgctata ctgctgtcga ttcgatacta acgccgccat cccattcgcc attcaggctg
     2761 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa
     2821 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt
     2881 tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac tatagggcga attggttaag
     2941 atccactttc acatttaagt tgtttttcta atccgcatat gatcaattca aggccgaata
     3001 agaaggctgg ctctgcacct tggtgatcaa ataattcgat agcttgtcgt aataatggcg
     3061 gcatactatc agtagtaggt gtttcccttt cttctttagc gacttgatgc tcttgatctt
     3121 ccaatacgca acctaaagta aaatgcccca cagcgctgag tgcatataat gcattctcta
     3181 gtgaaaaacc ttgttggcat aaaaaggcta attgattttc gagagtttca tactgttttt
     3241 ctgtaggccg tgtacctaaa tgtacttttg ctccatcgcg atgacttagt aaagcacatc
     3301 taaaactttt agcgttatta cgtaaaaaat cttgccagct ttccccttct aaagggcaaa
     3361 agtgagtatg gtgcctatct aacatctcaa tggctaaggc gtcgagcaaa gcccgcttat
     3421 tttttacatg ccaatacaat gtaggctgct ctacacctag cttctgggcg agtttacggg
     3481 ttgttaaacc ttcgattccg acctcattaa gcagctctaa tgcgctgtta atcactttac
     3541 ttttatctaa tctagacata tgaattcggg gccgcggagg ctggatcggt cccggtgtct
     3601 tctatggagg tcaaaacagc gtggatggcg tctccaggcg atctgacggt tcactaaacg
     3661 agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg tcaatggggc
     3721 ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca aactcccatt
     3781 gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc cacgcccatt
     3841 gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt agatgtactg
     3901 ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc
     3961 gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg
     4021 ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac
     4081 tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg
     4141 cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga
     4201 ccccgtaatt gattactatt aataactagt caataatcaa tgtcaacatg gcggtaatgt
     4261 tggacatgag ccaatataaa tgtacatatt atgatatgga tacaacgtat gcaatgggcc
     4321 aagctcctcg accccccggg ccccatctct tgccccttct aagctcgctg gactcgcaca
     4381 ttggctacgg tggctcgcgt cgctcgcttg gtcgctcctt caacgtaccc taacgttccg
     4441 gttaacgatt cttaaagaag cgaccaattt gttccaccgg cttctgtggg ctcaggggca
     4501 tgtcatggaa gattgagagg atcggcgctt cctccaggta tatatagagt gtccctatca
     4561 gtgatagaga cttgtttcag taagaatcaa ttagtattct acagtaaaca tcgcgggccg
     4621 ctcgggcccg gctagcatga gtaaaggaga agaacttttc actggagttg tcccaattct
     4681 tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag agggtgaagg
     4741 tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa aactacctgt
     4801 tccatggcca acacttgtca ctactttggg ttatggtcta atgtgctttg ctagataccc
     4861 agatcatatg aaacggcatg actttttcaa gagtgccatg cccgaaggtt atgtacagga
     4921 aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag tcaagtttga
     4981 aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag aagatggaaa
     5041 cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca tcatggcaga
     5101 caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg aagatggaag
     5161 cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc ctgtcctttt
     5221 accagacaac cattacctgt cctatcaatc taagctttcg aaagatccca acgaaaagag
     5281 agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg gcatggatga
     5341 actatacaaa agatcttaca gtttaaacga gctcgaattc aagggcgaat tctgcagata
     5401 tccatcacac tggcggccgc tcgagcatgc atctagaggg cccaattcgc cctatagtga
     5461 gtcgtattac aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     5521 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     5581 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggacgcgccc
     5641 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     5701 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     5761 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     5821 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     5881 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     5941 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     6001 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     6061 tttaacaaaa ttcagggcgc aagggctgct aaaggaagcg gaacacgtag aaagccagtc
     6121 cgcagaaacg gtgctgaccc cggatgaatg tcagctactg ggctatctgg acaagggaaa
     6181 acgcaagcgc aaagagaaag caggtagctt gcagtgggct tacatggcga tagctagact
     6241 gggcggtttt atggacagca agcgaaccgg aattgccagc tggggcgccc tctggtaagg
     6301 ttgggaagcc ctgcaaagta aactggatgg ctttcttgcc gccaaggatc tgatggcgca
     6361 ggggatcaag atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     6421 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     6481 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     6541 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
     6601 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     6661 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatccc
     6721 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     6781 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     6841 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     6901 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
     6961 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     7021 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     7081 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     7141 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgaa
     7201 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     7261 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     7321 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     7381 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     7441 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     7501 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     7561 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     7621 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     7681 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     7741 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     7801 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     7861 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     7921 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     7981 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     8041 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     8101 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     8161 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     8221 ccccgtagaa aagatcaaag gatcttc
//
