LOCUS       Exported                5885 bp ds-DNA     circular SYN 10-JUL-2024
DEFINITION  pCMVInt.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5885)
  AUTHORS   UNKNOWN
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 16, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..5885
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      19..325
                     /label=f1_origin
     gene            745..1605
                     /gene="Ampicillin"
                     /label=Ampicillin
     CDS             745..1605
                     /label=ORF frame 1
     misc_feature    complement(1707..1740)
                     /label=loxP
     rep_origin      1802..2421
                     /label=pBR322_origin
     promoter        complement(2693..2721)
                     /label=AmpR_promoter
     promoter        2868..2892
                     /label=lac_promoter
     misc_feature    2957..3244
                     /label=CAG_enhancer
     promoter        2962..3454
                     /label=CMV_immearly_promoter
     promoter        3412..3481
                     /label=CMV_promoter
     promoter        3424..3543
                     /label=CMV2_promoter
     promoter        3576..3597
                     /label=SP6 promoter
     CDS             3657..5156
                     /label=huOpt Bxb1
     promoter        complement(5223..5241)
                     /label=T7_promoter
     misc_feature    5330..5345
                     /label=SV40_int
     terminator      5631..5762
                     /label=SV40_PA_terminator
ORIGIN
        1 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
       61 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      121 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      181 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
      241 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
      301 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
      361 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
      421 aacgcgaatt ttaacaaaat attaacgttt acaatttcag gtggcacttt tcggggaaat
      481 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      541 ccaggtcttg gactggtgag aacggcttgc tcggcagctt cgatgtgtgc tggagggaga
      601 ataaaggtct aagatgtgcg atagagggaa gtcgcattga attatgtgct gtgtagggat
      661 cgctggtatc aaatatgtgt gcccacccct ggcatgagac aataaccctg ataaatgctt
      721 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
      781 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
      841 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
      901 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
      961 ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc
     1021 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     1081 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     1141 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     1201 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     1261 aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta
     1321 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     1381 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     1441 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     1501 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     1561 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     1621 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     1681 aagatccttt ttgataatct catgccataa cttcgtataa tgtatgctat acgaagttat
     1741 ggcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     1801 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     1861 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     1921 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg
     1981 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     2041 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     2101 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     2161 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagca ttgagaaagc
     2221 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     2281 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     2341 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     2401 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     2461 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     2521 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     2581 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     2641 agagcttgca attcgcgcgt ttttcaatat tattgaagca tttatcaggg ttattgtctc
     2701 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     2761 tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac attaacctat
     2821 aaaaataggc gtatacgagg ccctttcact cattaggcac cccaggcttt acactttata
     2881 gcttccggct cgtataatgt gtggaattgt gagcggataa caatttcaca caggaaacag
     2941 catcgtgcag gtcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
     3001 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
     3061 ccattgacgt caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt
     3121 gtatcatatg ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca
     3181 ttatgcccag tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt
     3241 catcgctatt accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt
     3301 tgactcacgg ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca
     3361 ccaaaatcaa cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg
     3421 cggtaggcgt gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat
     3481 cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag
     3541 cctccggact ctagcctagg cttttgcaaa aagctattta ggtgacacta tagaaggtac
     3601 gcctgcaggt accggtccgg aattcccggg tcgacgagct cactagtgcc gccaccatgc
     3661 gagccctcgt agtcatacgc ctttctcgcg taaccgatgc gacgactagt cccgagagac
     3721 aattggagtc ttgtcaacaa ttgtgtgcgc agcgaggctg ggacgtcgta ggagtcgcag
     3781 aggaccttga tgtctcagga gccgttgatc cttttgacag gaaaaggcga ccaaatcttg
     3841 cgagatggct tgcttttgag gagcagccat ttgatgttat agtagcatat agggtcgaca
     3901 gattgacgcg gtctatacga catctccagc agctggtcca ttgggccgaa gaccacaaga
     3961 aactggttgt gagcgcaacg gaggcacact ttgatacaac tactcctttc gcggctgttg
     4021 tgatcgcgct catgggtacc gtggctcaaa tggagcttga ggcaatcaaa gaacggaatc
     4081 gatcagctgc ccattttaat ataagagctg gtaaatatcg cggctctctg cctccctggg
     4141 ggtatcttcc gacaagagta gacggcgaat ggcgcctcgt tccggacccg gtacaacggg
     4201 agaggatact ggaagtatat caccgagtgg tagacaatca cgagccgctt cacttggtcg
     4261 cgcacgattt gaatagacgg ggcgtacttt ctcctaaaga ttatttcgcg caactgcaag
     4321 ggcgcgaacc gcaaggtaga gagtggagtg caacagccct caagagatca atgatctcag
     4381 aggctatgct ggggtacgcg acgctcaatg gcaaaacagt gcgagacgat gatggtgccc
     4441 ccctcgttag agccgaacct atcctgactc gggaacaact cgaggccttg cgggccgagc
     4501 tggtgaaaac ttcacgcgcc aaaccagctg tttcaacacc ctcacttctt ctgcgagttc
     4561 tgttctgtgc agtctgcgga gagccggcgt ataaatttgc aggtggtggg agaaagcatc
     4621 cgaggtatcg atgccgaagc atggggtttc ccaaacattg cggcaatggc accgtcgcta
     4681 tggccgagtg ggatgcattt tgtgaggaac aggtattgga ccttttgggg gacgcagaga
     4741 ggttggagaa ggtttgggtc gcaggatccg attctgcggt ggagcttgcc gaagtcaacg
     4801 ctgagctcgt ggacctcacg tccctcattg gtagcccagc gtaccgcgcg ggttcccctc
     4861 agagagaggc gcttgatgcc cgcatcgccg ctcttgcggc cagacaagag gagcttgagg
     4921 gactggaggc gaggccaagc ggttgggagt ggagggaaac gggccagcga ttcggcgatt
     4981 ggtggcgaga gcaggacaca gctgcgaaga acacttggct tagatccatg aatgttcggt
     5041 tgacctttga tgtgaggggc ggtctgacgc gaacgatcga cttcggcgac cttcaagagt
     5101 acgaacagca tcttaggctg gggtcagtag tcgagcgctt gcacaccggg atgtcgtagc
     5161 gagacacccg gatccctcga ggggcccaag cttacgcgtg catgcgacgt catagctctc
     5221 tccctatagt gagtcgtatt ataagctagc ttgggatctt tgtgaaggaa ccttacttct
     5281 gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag gtaaatataa
     5341 aatttttaag tgtataatgt gttaaactag ctgcatatgc ttgctgcttg agagttttgc
     5401 ttactgagta tgatttatga aaatattata cacaggagct agtgattcta attgtttgtg
     5461 tattttagat tcacagtccc aaggctcatt tcaggcccct cagtcctcac agtctgttca
     5521 tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     5581 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     5641 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5701 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     5761 tcgatcctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattggctgg
     5821 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     5881 gaatg
//
