LOCUS       Exported                2376 bp ds-DNA     circular SYN 14-JUL-2025
DEFINITION  pFYF1320 withBsmBI spacer insertion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   UNKNOWN
  TITLE     Direct Submission
  JOURNAL   Exported Wednesday, Jul 16, 2025 from SnapGene 5.0.8
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     promoter        67..330
                     /standard_name="hU6"
                     /label=hU6 promoter
     misc_feature    331..523
                     /label=pegRNA ROSA spacer4a Bxb1 attB38
     misc_feature    331..350
                     /label=spacer Rosa4a
     misc_feature    351..436
                     /label=cr722 scaffold
     misc_feature    437..465
                     /label=RT template
     misc_feature    446..447
                     /label=GT core
     misc_feature    466..478
                     /label=PBS
     misc_feature    479..486
                     /label=linker
     misc_feature    487..523
                     /label=tevopreQ1
     terminator      524..530
                     /standard_name="U6 terminator"
                     /label=U6 terminator
     misc_feature    complement(612..1231)
                     /standard_name="pBR322 origin"
                     /label=pBR322 origin
                     /note="Geneious type: origin of replication"
     CDS             complement(1386..2246)
                     /standard_name="amp marker"
                     /label=amp marker
     promoter        complement(2288..2316)
                     /standard_name="amp prom"
                     /label=amp prom promoter
ORIGIN
        1 gacgtcgcta gctgtacaaa aaagcaggct ttaaaggaac caattcagtc gactggatcc
       61 ggtaccaagg tcgggcagga agagggccta tttcccatga ttccttcata tttgcatata
      121 cgatacaagg ctgttagaga gataattaga attaatttga ctgtaaacac aaagatatta
      181 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
      241 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
      301 ttatatatct tgtggaaagg acgaaacacc atgcctggta gggatgcaaa gtttaagagc
      361 taagctggaa acagcatagc aagtttaaat aaggctagtc cgttatcaac tcgaaagagt
      421 ggcaccgagt cggtgcacga cggcggtctc cgtcgtcagg atcatgcatc cctaccagaa
      481 cttcgtcgcg gttctatcta gttacgcgtt aaaccaacta gaattttttt aagcttgggc
      541 cgctcgaggt acctctctac atatgacatg tgagcaaaag gccagcaaaa ggccaggaac
      601 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
      661 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
      721 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
      781 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
      841 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
      901 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
      961 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     1021 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     1081 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     1141 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     1201 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     1261 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     1321 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     1381 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     1441 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     1501 ggccccagtg ctgcaatgat accgcgagat ccacgctcac cggctccaga tttatcagca
     1561 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     1621 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     1681 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     1741 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     1801 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     1861 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     1921 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     1981 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     2041 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     2101 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     2161 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     2221 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     2281 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     2341 ggggttccgc gcacatttcc ccgaaaagtg ccacct
//
