LOCUS       LZRS-MS-NEO_PIWI       15991 bp    DNA     circular SYN 16-JUL-2025
DEFINITION  LZRS_myc-PIWIL2-FLAG  (16,021 bp).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15991)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 16, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..15991
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             541..1010
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    1075..1432
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
     CDS             1497..1913
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     misc_feature    1923..2296
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     misc_feature    2320..2325
                     /label=EcoRI site
     misc_feature    2344
                     /label=IVS [Split]
                     /note="IVS [Split]"
     misc_feature    2344
                     /label=MCS [Split]
                     /note="MCS [Split]"
     misc_feature    2344
                     /label=AFEI [Split]
                     /note="AFEI [Split]"
     misc_feature    2345..5263
                     /label=PIWIL2 full length
     CDS             4031..4042
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(4947..4958)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    5264..5338
                     /label=3xFLAG tag
     misc_feature    5339
                     /label=EcoRI site
     misc_feature    5340..5396
                     /label=IVS [Split]
                     /note="IVS [Split]"
     misc_feature    join(5424..5998,5999..6001,6002..6010)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     CDS             6033..6836
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     LTR             6932..7525
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     rep_origin      complement(7827..8415)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     polyA_signal    8538..8672
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             complement(8902..9501)
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     promoter        complement(9584..10077)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             10502..12427
                     /codon_start=1
                     /product="Epstein-Barr nuclear antigen 1, also known as 
                     EBNA-1"
                     /label=EBNA1
                     /note="crucial for latent viral infection, and for episomal
                     amplification of vectors containing the oriP origin (Okita 
                     et al., 2013)"
                     /translation="MSDEGPGTGPGNGLGEKGDTSGPEGSGGSGPQRRGGDNHGRGRGR
                     GRGRGGGRPGAPGGSGSGPRHRDGVRRGEKRPSCIGCKGTHGGTGAGAGAGGAGAGGAG
                     AGGGAGAGGGAGGAGGAGGAGAGGGAGAGGGAGGAGGAGAGGGAGAGGGAGGAGAGGGA
                     GGAGGAGAGGGAGAGGGAGGAGAGGGAGGAGGAGAGGGAGAGGAGGAGGAGAGGAGAGG
                     GAGGAGGAGAGGAGAGGAGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGGAGAG
                     GGAGGAGAGGAGGAGAGGAGGAGAGGAGGAGAGGGAGAGGAGAGGGGRGRGGSGGRGRG
                     GSGGRGRGGSGGRRGRGRERARGGSRERARGRGRGRGEKRPRSPSSQSSSSGSPPRRPP
                     PGRRPFFHPVGEADYFEYHQEGGPDGEPDVPPGAIEQGPADDPGEGPSTGPRGQGDGGR
                     RKKGGWFGKHRGQGGSNPKFENIAEGLRALLARSHVERTTDEGTWVAGVFVYGGSKTSL
                     YNLRRGTALAIPQCRLTPLSRLPFGMAPGPGPQPGPLRESIVCYFMVFLQTHIFAEVLK
                     DAIKDLVMTKPAPTCNIRVTVCSFDDGVDLPPWFPPMVEGAAAEGDDGDDGDEGGDGDE
                     GEEGQE"
     rep_origin      12733..14522
                     /label=oriP
                     /note="Epstein-Barr virus oriP replication origin (Yates et
                     al., 2000)"
     CDS             complement(join(15055..15846,15847..15915))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 atcacgaggc cctttcgtct tcaagaacag ctttgctctt aggagtttcc taatacatcc
       61 caaactcaaa tatataaagc atttgacttg ttctatgccc tagggggcgg ggggaagcta
      121 agccagcttt ttttaacatt taaaatgtta attccatttt aaatgcacag atgtttttat
      181 ttcataaggg tttcaatgtg catgaatgct gcaatattcc tgttaccaaa gctagtataa
      241 ataaaaatag ataaacgtgg aaattactta gagtttctgt cattaacgtt tccttcctca
      301 gttgacaaca taaatgcgct gctgagaagc cagtttgcat ctgtcaggat caatttccca
      361 ttatgccagt catattaatt actagtcaat tagttgattt ttatttttga catatacatg
      421 tgaaagaccc cacctgtagg tttggcaagc tagcttaagt aacgccattt tgcaaggcat
      481 ggaaaaatac ataactgaga atagaaaagt tcagatcaag gtcaggaaca gatggaacag
      541 ctgaatatgg gccaaagcgg atatctgtgg taagcagttc ctgccccggc tcagggccaa
      601 gaacagatgg aacagctgaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc
      661 ccggctcagg gccaagaaca gatggtcccc agatgcggtc cagccctcag cagtttctag
      721 agaaccatca gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg
      781 aactaaccaa tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat
      841 aaaagagccc acaacccctc actcggggcg ccagtcctcc gattgactga gtcgcccggg
      901 tacccgtgta tccaataaac cctcttgcag ttgcatccga cttgtggtct cgctgttcct
      961 tgggagggtc tcctctgagt gattgactac ccgtcagcgg gggtctttca tttgggggct
     1021 cgtccgggat cgggagaccc ctgcccaggg accaccgacc caccaccggg aggtaagctg
     1081 gccagcaact tatctgtgtc tgtccgattg tctagtgtct atgactgatt ttatgcgcct
     1141 gcgtcggtac tagttagcta actagctctg tatctggcgg acccgtggtg gaactgacga
     1201 gttcggaaca cccggccgca accctgggag acgtcccagg gacttcgggg gccgtttttg
     1261 tggcccgacc tgagtccaaa aatcccgatc gttttggact ctttggtgca ccccccttag
     1321 aggagggata tgtggttctg gtaggagacg agaacctaaa acagttcccg cctccgtctg
     1381 aatttttgct ttcggtttgg gaccgaagcc gcgccgcgcg tcttgtctgc tgcagcatcg
     1441 ttctgtgttg tctctgtctg actgtgtttc tgtatttgtc tgaaaatatg ggcccgggcc
     1501 agactgttac cactccctta agtttgacct taggtcactg gaaagatgtc gagcggatcg
     1561 ctcacaacca gtcggtagat gtcaagaaga gacgttgggt taccttctgc tctgcagaat
     1621 ggccaacctt taacgtcgga tggccgcgag acggcacctt taaccgagac ctcatcaccc
     1681 aggttaagat caaggtcttt tcacctggcc cgcatggaca cccagaccag gtcccctaca
     1741 tcgtgacctg ggaagccttg gcttttgacc cccctccctg ggtcaagccc tttgtacacc
     1801 ctaagcctcc gcctcctctt cctccatccg ccccgtctct cccccttgaa cctcctcgtt
     1861 cgaccccgcc tcgatcctcc ctttatccag ccctcactcc ttctctaggc gcccccatat
     1921 ggccatatga gatcttatat ggggcacccc cgccccttgt aaacttccct gaccctgaca
     1981 tgacaagagt tactaacagc ccctctctcc aagctcactt acaggctctc tacttagtcc
     2041 agcacgaagt ctggagacct ctggcggcac gtaccaagaa caactggacc gaccggtggt
     2101 acctcaccct taccgagtcg gcgacacagt gtgggtccgc cgacaccaga ctaagaacct
     2161 agaacctcgc tggaaaggac cttacacagt cctgctgacc acccccaccg ccctcaaagt
     2221 agacggcatc gcagcttgga tacacgccgc ccacgtgaag gctgccgacc ccgggggtgg
     2281 accatcctct agactgccgg atcccagtgt ggtggtaggg aattcaaacg ggccctctag
     2341 accaatggat cctttccgac catcgttcag gggccagtct cctatccacc catcccagtg
     2401 ccaggctgta cggatgccag gctgttggcc acaagcttct aaacctttgg acccagctct
     2461 gggcagggga gcacctgcag gcagaggcca tgtatttgga aagccagagg aaccaagcac
     2521 acagaggggg ccagcacaaa gggagtctgt gggtttggtc tccatgttcc gaggcctggg
     2581 cattgaaaca gtttctaaga cccctctgaa acgggaaatg cttccatcag gtagaggcat
     2641 tttaggtcga ggcttgtctg ctaatctggt acgcaaggac agggaggaac tctctcccac
     2701 tttttgggat ccaaaagtgt tggcggctgg ggacagcaag atggcagaga cctccgttgg
     2761 ttggagtagg acgcttggaa gagggagttc agatgcgtct ttattaccac tgggaagagc
     2821 agcaggtggt atcagcagag aagtggacaa gcctccctgt accttcagca caccgtcccg
     2881 gggtcccccg cagctgtcat caccaccagc tctgccccag tctcccctgc actctccaga
     2941 tcgccctctg gtcctgactg tggaacacaa ggaaaaagag cttattgtga agcaaggatc
     3001 aaaaggaaca cctcagtctt tgggactgaa cctcgtcaaa atacagtgtc ataatgaagc
     3061 agtttatcaa tatcatgtga ctttcagccc caatgtggag tgcaaaagca tgaggttcgg
     3121 catgttgaag gaccatcaag ctgtcaccgg caacgtcact gcgtttgatg gatctattct
     3181 ctatctgcct gttaagcttc aacaagttct tgagttaaaa agtcaaagga aaacagacag
     3241 tgctgaaatc agcattaaga ttcagatgac aaagatcctg gagccctgct ctgacctgtg
     3301 cattcccttc tacaatgttg ttttccgtcg ggtaatgaaa cttttagata tgaagcttgt
     3361 ggggagaaac ttttatgacc ctacaagtgc tatggtacta cagcaacaca gattgcagat
     3421 ctggccaggc tatgcagcta gcatccgaag gacagatgga gggctcttcc tgctagctga
     3481 tgtctcccat aaggtcattc ggaatgactg tgtgctggat gtcatgcatg ccatttatca
     3541 gcagaataaa gaacacttcc aggatgagtg tactaagctt ctggttggca atattgttat
     3601 cacccgatat aacaatcgta cctatcgtat tgatgatgtg gattggaata agactccaaa
     3661 ggatagcttc acgatgtctg atgggaaaga gatcacattc ttggaatact acagcaaaaa
     3721 ttatgggatc acagttaagg aagaggacca gccattgctg attcacaggc ccagtgagag
     3781 acaggataat catgggatgc tgctaaaagg ggaaatcctg ctgctgcctg agctttcttt
     3841 tatgaccgga atcccagaga agatgaagaa ggacttcaga gccatgaagg atttggctca
     3901 gcaaatcaat ctgagcccca agcaacacca tagtgctttg gaatgcttgc tgcaaagaat
     3961 tgcaaagaac gaggcagcca ccaatgaact gatgcgttgg gggctccgtc tgcaaaagga
     4021 tgtacataag attgaaggac gtgttctgcc aatggaaaga attaacttaa aaaatacttc
     4081 gtttatcaca tctcaggaac taaactgggt taaggaagta accagagacc cttccatctt
     4141 gactatcccc atgcatttct gggcactttt ttacccaaag agagcaatgg accaggctcg
     4201 agaactggtc aacatgttgg agaagatagc cggccccatt ggcatgcgta tgagcccacc
     4261 ggcctgggtt gaactaaagg atgaccgaat agagacttat gtcagaacca ttcaatccac
     4321 gttaggagct gaggggaaga tacagatggt tgtttgcatc atcatgggcc cacgtgatga
     4381 tctctatggg gccatcaaga agctgtgctg tgtgcagtcc ccagtgccct cccaggttgt
     4441 caatgttcga accattggtc agcccaccag gcttcggagt gtggcccaga agattttact
     4501 tcagattaac tgtaaattgg gtggtgagct ctggggagtg gatattcctc tgaaacagtt
     4561 aatggtgatc gggatggatg tttaccatga ccccagtaga ggcatgcgct ccgtggttgg
     4621 cttcgtggca agcatcaatc tcaccctcac aaaatggtat tcccgggtgg tgttccagat
     4681 gccgcatcag gagattgtgg acagcctgaa gctatgcctc gtgggctcct taaaaaagtt
     4741 ttatgaggtg aaccactgtc taccagagaa gattgtggtg taccgtgatg gagtgtctga
     4801 tggccaactg aagacagttg ccaactatga gattcctcaa ctacagaagt gttttgaagc
     4861 ttttgagaat tatcagccca agatggtggt gtttgtagtt cagaagaaaa tcagtactaa
     4921 tctatatctg gctgctcctc agaactttgt aactcccact cctggaactg tggtagatca
     4981 tacaataaca agctgtgagt gggtggattt ctatcttctt gcccatcatg tacggcaggg
     5041 ctgtggcatt cctacgcatt atgtctgtgt tctcaacacc gcaaacctga gccctgatca
     5101 tatgcagagg ctgactttca aactgtgcca catgtactgg aattggcctg gcaccatcag
     5161 agttccagct ccttgcaagt atgcccacaa gctagctttc ctgtcaggac acatcttgca
     5221 tcatgaacca gccatccagc tgtgcgagaa cctgttcttc ctggattaca aggatgacga
     5281 cgataaggac tataaggacg atgatgacaa ggactacaaa gatgatgacg ataaatgaga
     5341 attctgcaag ccgcgatcgc agcgctggcc ggtccggccg gtccaactgc aggtcgagca
     5401 tgcatctagg gcggccaatt ccgcccctct ccctcccccc cccctaacgt tactggccga
     5461 agccgcttgg aataaggccg gtgtgcgttt gtctatatgt gattttccac catattgccg
     5521 tcttttggca atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg
     5581 ggtctttccc ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt
     5641 cctctggaag cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac
     5701 cccccacctg gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca
     5761 aaggcggcac aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg
     5821 ctctcctcaa gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg
     5881 ggatctgatc tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa
     5941 cgtctaggcc ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct
     6001 tgccacaacc cgggataatt cctgcagcca atatgggatc ggccattgaa caagatggat
     6061 tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac
     6121 agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc
     6181 tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc
     6241 tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag
     6301 cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc
     6361 ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg
     6421 atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc
     6481 ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc
     6541 cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgatgat ctcgtcgtga
     6601 cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca
     6661 tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg
     6721 atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg
     6781 ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagggg
     6841 atcaattctc tagagctcgc tgatcgcggc cgccagcaca gtggtcgacg ataaaataaa
     6901 agattttatt tagtctccag aaaaaggggg gaatgaaaga ccccacctgt aggtttggca
     6961 agctagctta agtaacgcca ttttgcaagg catggaaaaa tacataactg agaatagaga
     7021 agttcagatc aaggtcagga acagatggaa cagctgaata tgggccaaac aggatatctg
     7081 tggtaagcag ttcctgcccc ggctcagggc caagaacaga tggaacagct gaatatgggc
     7141 caaacaggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggtc
     7201 cccagatgcg gtccagccct cagcagtttc tagagaacca tcagatgttt ccagggtgcc
     7261 ccaaggacct gaaatgaccc tgtgccttat ttgaactaac caatcagttc gcttctcgct
     7321 tctgttcgcg cgcttctgct ccccgagctc aataaaagag cccacaaccc ctcactcggg
     7381 gcgccagtcc tccgattgac tgagtcgccc gggtacccgt gtatccaata aaccctcttg
     7441 cagttgcatc cgacttgtgg tctcgctgtt ccttgggagg gtctcctctg agtgattgac
     7501 tacccgtcag cgggggtctt tcattcacat gcagcatgta tcaaaattaa tttggttttt
     7561 tttcttaagt atttacatta aatggccata gttgcattaa tgaatcggcc aacgcgcggg
     7621 gagaggcggt ttgcgtattg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7681 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7741 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7801 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7861 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7921 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7981 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8041 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8101 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8161 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8221 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8281 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8341 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8401 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8461 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8521 cttttgcggc cgaattagat ccagacatga taagatacat tgatgagttt ggacaaacca
     8581 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     8641 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     8701 ttcaggttca gggggaggtg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     8761 gtatggctga ttatgatcct ctagagtcgg tgggcctcgg gggcgggtgc ggggtcggcg
     8821 gggccgcccg ggtcggcttc ggtcggagcc atggggtcgt gcgctccttt cggtcgggcg
     8881 ctgcgggtcg tggggcgggc gtcaggcacc gggcttgcgg gtcatgcacc aggtcgcgcg
     8941 gtccttcggg cactcgacgt cggcggtgac ggtgaagccg agccgctcgt agaaggggag
     9001 gttgcggggc gcggaggtct ccaggaaggc gggcaccccg gcgcgctcgg ccgcctccac
     9061 tccggggagc acgacggcgc tgcccagacc cttgccctgg tggtcgggcg agacgccgac
     9121 ggtggccagg aaccacgcgg gctccttggg ccggtgcggc gccaggaggc cttccatctg
     9181 ttgctgcgcg gccagccggg aaccgctcaa ctcggccatg cgcgggccga tctcggcgaa
     9241 caccgccccc gcttcgacgc tctccggcgt ggtccagacc gccaccgcgg cgccgtcgtc
     9301 cgcgacccac accttgccga tgtcgagccc gacgcgcgtg aggaagagtt cttgcagctc
     9361 ggtgacccgc tcgatgtggc ggtccgggtc gacggtgtgg cgcgtggcgg ggtagtcggc
     9421 gaacgcggcg gcgagggtgc gtacggcccg ggggacgtcg tcgcgggtgg cgaggcgcac
     9481 cgtgggcttg tactcggtca tggaaggtcg tctccttgtg aggggtcagg ggcgtgggtc
     9541 aggggatggt ggcggcaccg gtcgtggcgg ccgacctgca ggtcgaaagg cccggagatg
     9601 aggaagagga gaacagcgcg gcagacgtgc gcttttgaag cgtgcagaat gccgggctcc
     9661 ggaggacctt cgcgcccgcc ccgcccctga gcccgcccct gagcccgccc ccggacccac
     9721 cccttcccag cctctgagcc cagaaagcga aggagcaaag ctgctattgg ccgctgcccc
     9781 aaaggcctac ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac
     9841 gtgctacttc catttgtcac gtcctgcacg acgcgagctg cggggcgggg gggaacttcc
     9901 tgactagggg aggagtagaa ggtggcgcga aggggccacc aaagaacgga gccggttggc
     9961 gctaccggtg gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc gccaagtgcc
    10021 agcggggctg caaagcgcat gctccagact gccttgggaa aagcgcctcc cctacccggt
    10081 agaattaatt ctcatgtttg acagcttatc atcgatagat cctcacaggc cgcacccagc
    10141 ttttcttccg ttgccccagt agcatctctg tctggtgacc ttgaagagga agaggagggg
    10201 tcccgagaat ccccatccct accgtccagc aaaaaggggg acgaggaatt tgaggcctgg
    10261 cttgaggctc aggacgcaaa tcttgaggat gttcagcggg agttttccgg gctgcgagta
    10321 attggtgatg aggacgagga tggttcggag gatggggaat tttcagacct ggatctgtct
    10381 gacagcgacc atgaagggga tgagggtggg ggggctgttg gagggggcag gagtctgcac
    10441 tccctgtatt cactgagcgt cgtctaataa agatgtctat tgatctcttt tagtgtgaat
    10501 catgtctgac gaggggccag gtacaggacc tggaaatggc ctaggagaga agggagacac
    10561 atctggacca gaaggctccg gcggcagtgg acctcaaaga agagggggtg ataaccatgg
    10621 acgaggacgg ggaagaggac gaggacgagg aggcggaaga ccaggagccc cgggcggctc
    10681 aggatcaggg ccaagacata gagatggtgt ccggagaggg gaaaaacgtc caagttgcat
    10741 tggctgcaaa gggacccacg gtggaacagg agcaggagca ggagcgggag gggcaggagc
    10801 aggaggggca ggagcaggag gaggggcagg agcaggagga ggggcaggag gggcaggagg
    10861 ggcaggaggg gcaggagcag gaggaggggc aggagcagga ggaggggcag gaggggcagg
    10921 aggggcagga gcaggaggag gggcaggagc aggaggaggg gcaggagggg caggagcagg
    10981 aggaggggca ggaggggcag gaggggcagg agcaggagga ggggcaggag caggaggagg
    11041 ggcaggaggg gcaggagcag gaggaggggc aggaggggca ggaggggcag gagcaggagg
    11101 aggggcagga gcaggagggg caggaggggc aggaggggca ggagcaggag gggcaggagc
    11161 aggaggaggg gcaggagggg caggaggggc aggagcagga ggggcaggag caggaggggc
    11221 aggagcagga ggggcaggag caggaggggc aggaggggca ggagcaggag gggcaggagg
    11281 ggcaggagca ggaggggcag gaggggcagg agcaggagga ggggcaggag gggcaggagc
    11341 aggaggaggg gcaggagggg caggagcagg aggggcagga ggggcaggag caggaggggc
    11401 aggaggggca ggagcaggag gggcaggagg ggcaggagca ggaggagggg caggagcagg
    11461 aggggcagga gcaggaggtg gaggccgggg tcgaggaggc agtggaggcc ggggtcgagg
    11521 aggtagtgga ggccggggtc gaggaggtag tggaggccgc cggggtagag gacgtgaaag
    11581 agccaggggg ggaagtcgtg aaagagccag ggggagaggt cgtggacgtg gagaaaagag
    11641 gcccaggagt cccagtagtc agtcatcatc atccgggtct ccaccgcgca ggccccctcc
    11701 aggtagaagg ccatttttcc accctgtagg ggaagccgat tattttgaat accaccaaga
    11761 aggtggccca gatggtgagc ctgacgtgcc cccgggagcg atagagcagg gccccgcaga
    11821 tgacccagga gaaggcccaa gcactggacc ccggggtcag ggtgatggag gcaggcgcaa
    11881 aaaaggaggg tggtttggaa agcatcgtgg tcaaggaggt tccaacccga aatttgagaa
    11941 cattgcagaa ggtttaagag ctctcctggc taggagtcac gtagaaagga ctaccgacga
    12001 aggaacttgg gtcgccggtg tgttcgtata tggaggtagt aagacctccc tttacaacct
    12061 aaggcgagga actgcccttg ctattccaca atgtcgtctt acaccattga gtcgtctccc
    12121 ctttggaatg gcccctggac ccggcccaca acctggcccg ctaagggagt ccattgtctg
    12181 ttatttcatg gtctttttac aaactcatat atttgctgag gttttgaagg atgcgattaa
    12241 ggaccttgtt atgacaaagc ccgctcctac ctgcaatatc agggtgactg tgtgcagctt
    12301 tgacgatgga gtagatttgc ctccctggtt tccacctatg gtggaagggg ctgccgcgga
    12361 gggtgatgac ggagatgacg gagatgaagg aggtgatgga gatgagggtg aggaagggca
    12421 ggagtgatgt aacttgttag gagacgccct caatcgtatt aaaagccgtg tattcccccg
    12481 cactaaagaa taaatcccca gtagacatca tgcgtgctgt tggtgtattt ctggccatct
    12541 gtcttgtcac cattttcgtc ctcccaacat ggggcaattg ggcataccca tgttgtcacg
    12601 tcactcagct ccgcgctcaa caccttctcg cgttggaaaa cattagcgac atttacctgg
    12661 tgagcaatca gacatgcgac ggctttagcc tggcctcctt aaattcacct aagaatggga
    12721 gcaaccagca tgcaggaaaa ggacaagcag cgaaaattca cgcccccttg ggaggtggcg
    12781 gcatatgcaa aggatagcac tcccactcta ctactgggta tcatatgctg actgtatatg
    12841 catgaggata gcatatgcta cccggataca gattaggata gcatatacta cccagatata
    12901 gattaggata gcatatgcta cccagatata gattaggata gcctatgcta cccagatata
    12961 aattaggata gcatatacta cccagatata gattaggata gcatatgcta cccagatata
    13021 gattaggata gcctatgcta cccagatata gattaggata gcatatgcta cccagatata
    13081 gattaggata gcatatgcta tccagatatt tgggtagtat atgctaccca gatataaatt
    13141 aggatagcat atactaccct aatctctatt aggatagcat atgctacccg gatacagatt
    13201 aggatagcat atactaccca gatatagatt aggatagcat atgctaccca gatatagatt
    13261 aggatagcct atgctaccca gatataaatt aggatagcat atactaccca gatatagatt
    13321 aggatagcat atgctaccca gatatagatt aggatagcct atgctaccca gatatagatt
    13381 aggatagcat atgctatcca gatatttggg tagtatatgc tacccatggc aacattagcc
    13441 caccgtgctc tcagcgacct cgtgaatatg aggaccaaca accctgtgct tggcgctcag
    13501 gcgcaagtgt gtgtaatttg tcctccagat cgcagcaatc gcgcccctat cttggcccgc
    13561 ccacctactt atgcaggtat tccccggggt gccattagtg gttttgtggg caagtggttt
    13621 gaccgcagtg gttagcgggg ttacaatcag ccaagttatt acacccttat tttacagtcc
    13681 aaaaccgcag ggcggcgtgt gggggctgac gcgtgccccc actccacaat ttcaaaaaaa
    13741 agagtggcca cttgtctttg tttatgggcc ccattggcgt ggagccccgt ttaattttcg
    13801 ggggtgttag agacaaccag tggagtccgc tgctgtcggc gtccactctc tttccccttg
    13861 ttacaaatag agtgtaacaa catggttcac ctgtcttggt ccctgcctgg gacacatctt
    13921 aataacccca gtatcatatt gcactaggat tatgtgttgc ccatagccat aaattcgtgt
    13981 gagatggaca tccagtcttt acggcttgtc cccaccccat ggatttctat tgttaaagat
    14041 attcagaatg tttcattcct acactagtat ttattgccca aggggtttgt gagggttata
    14101 ttggtgtcat agcacaatgc caccactgaa ccccccgtcc aaattttatt ctgggggcgt
    14161 cacctgaaac cttgttttcg agcacctcac atacacctta ctgttcacaa ctcagcagtt
    14221 attctattag ctaaacgaag gagaatgaag aagcaggcga agattcagga gagttcactg
    14281 cccgctcctt gatcttcagc cactgccctt gtgactaaaa tggttcacta ccctcgtgga
    14341 atcctgaccc catgtaaata aaaccgtgac agctcatggg gtgggagata tcgctgttcc
    14401 ttaggaccct tttactaacc ctaattcgat agcatatgct tcccgttggg taacatatgc
    14461 tattgaatta gggttagtct ggatagtata tactactacc cgggaagcat atgctacccg
    14521 tttagggtta acaagggggc cttataaaca ctattgctaa tgccctcttg agggtccgct
    14581 tatcggtagc tacacaggcc cctctgattg acgttggtgt agcctcccgt agtcttcctg
    14641 ggcccctggg aggtacatgt cccccagcat tggtgtaaga gcttcagcca agagttacac
    14701 ataaaggcaa tgttgtgttg cagtccacag actgcaaagt ctgctccagg atgaaagcca
    14761 ctcagtgttg gcaaatgtgc acatccattt ataaggatgt caactacagt cagagaaccc
    14821 ctttgtgttt ggtccccccc cgtgtcacat gtggaacagg gcccagttgg caagttgtac
    14881 caaccaactg aagggattac atgcactgcc cgtgaccaat acaaaacaaa agcgctcctc
    14941 gtaccagcga agaaggggca gagatgccgt agtcaggttt agttcgtccg gcggcggggg
    15001 atctggccgc tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
    15061 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
    15121 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
    15181 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
    15241 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
    15301 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
    15361 tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
    15421 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
    15481 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
    15541 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
    15601 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
    15661 cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
    15721 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
    15781 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
    15841 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
    15901 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
    15961 tctcatgaca ttaacctata aaaataggcg t
//
