LOCUS       pv1210                 13113 bp    DNA     circular SYN 19-MAR-2025
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 13113)
  AUTHORS   Dimitrios
  TITLE     Direct Submission
  JOURNAL   Exported Jul 30, 2025 from SnapGene 8.1.1
            https://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..13113
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     primer_bind     660..705
                     /label=ENO1p_sF
                     /note="color: black; sequence: 
                     GCGAATTGAGGCCTGCATGCGGTACCCCAGGATTCTACGCGCATTG"
     primer_bind     660..705
                     /label=Fragment.FOR
                     /note="color: black; sequence: 
                     gcgaattgaggcctgcatgcggtaccCCAGGATTCTACGCGCATTG"
     promoter        686..1062
                     /label=CauENO1p
                     /note="color: #ffff00; direction: RIGHT"
     primer_bind     complement(1030..1082)
                     /label=Eno1p_sR
                     /note="color: black; sequence: 
                     ATACTATACTTTTTATCCATCCCGGGGATGAAAATTAAGTTTGGATAGGGAGG"
     primer_bind     complement(1030..1082)
                     /label=Fragment.REV
                     /note="color: black; sequence: 
                     atactatactttttatccatcccgggGATGAAAATTAAGTTTGGATAGGGAGG"
     CDS             1063..5319
                     /codon_start=1
                     /label=CaCas9
                     /note="color: #0000ff"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGDEGADPKKKRKVDPKKKRKVDPKKKRKVDYKDHDG
                     DYKDHDIDYKDDDDK*"
     primer_bind     complement(1218..1237)
                     /label=C1883
                     /note="color: black; sequence: cagtttcaccggagtcgaac"
     CDS             5179..5199
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             5203..5223
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             5227..5247
                     /codon_start=1
                     /product="nuclear localization signal of SV40 large T 
                     antigen"
                     /label=SV40 NLS
                     /note="color: #993366"
                     /translation="PKKKRKV"
     CDS             5248..5313
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /note="color: #cc99b2
                     Cleavage site after base 5313"
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      complement(5378..5625)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1term
                     /label=CYC1 terminator
                     /note="color: #0000ff; direction: LEFT"
     protein_bind    complement(5645..5678)
                     /label=FRT
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
                     /note="color: #000000; direction: LEFT"
     misc_feature    5690..6774
                     /label=SAP2 promoter
                     /note="color: #ffcc99"
     CDS             6775..8046
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=ScFLP
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /note="color: #993366"
                     /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     terminator      8047..8440
                     /label=ACT1term
                     /note="color: #99ccff"
     terminator      8441..8670
                     /label=AgTEFterm
                     /label=TEF terminator
                     /note="color: #800080"
     CDS             complement(8685..9257)
                     /codon_start=1
                     /label=NAT1
                     /note="color: #ff6600"
                     /translation="MSTTLDDTAYRYRTSVPGDAEAIEALDGSFTTDTVFRVTATGDGF
                     TLREVPVDPPLTKVFPDDESDDESDAGEDGDPDSRTFVAYGDDGDLAGFVVVSYSGWNR
                     RLTVEDIEVAPEHRGHGVGRALMGLATEFARERGAGHLWLEVTNVNAPAIHAYRRMGFT
                     LCGLDTALYDGTASDGEQALYMSMPCP"
     misc_feature    9271..9615
                     /label=AgTEF2 prom
                     /label=AgTEF2 prom.
                     /note="color: #800080"
     primer_bind     9638..9682
                     /label=Primer 1
                     /note="color: black; sequence: 
                     ACGAGGCAAGCTTGATGTGCGGCCGCACAGACTCAATCAACGAAG"
     primer_bind     9638..9682
                     /label=SNR52p_FWD
                     /note="color: black; sequence: 
                     ACGAGGCAAGCTTGATGTGCGGCCGCACAGACTCAATCAACGAAG"
     primer_bind     9638..9682
                     /label=Fragment 1.FOR
                     /note="color: black; sequence: 
                     ACGAGGCAAGCTTGATGTGCGGCCGCACAGACTCAATCAACGAAG"
     misc_feature    9664..10225
                     /label=CauSNR52p
                     /note="color: #ff00ff; direction: RIGHT"
     primer_bind     complement(10207..10244)
                     /label=SNR52p_REV
                     /note="color: black; sequence: 
                     CGAGACGGAATTCCGTCTCCTGTTTTCTGCTGAGGGAG"
     primer_bind     complement(10207..10244)
                     /label=Fragment 1.REV
                     /note="color: black; sequence: 
                     CGAGACGGAATTCCGTCTCCTGTTTTCTGCTGAGGGAG"
     primer_bind     10225..10250
                     /label=sgRNA_polyT_FRT_FWD
                     /note="color: black; sequence: GGAGACGGAATTCCGTCTCGTTTTAG"
     primer_bind     10225..10250
                     /label=Fragment 2.FOR
                     /note="color: black; sequence: GGAGACGGAATTCCGTCTCGTTTTAG"
     misc_feature    10244..10332
                     /label=gRNA scaffold
                     /note="color: #00ccff; direction: RIGHT"
     primer_bind     complement(10389..10409)
                     /label=sgRNA_polyT_FRT_REV
                     /note="color: black; sequence: ATAGGAACTTCACGCGGTGGC"
     primer_bind     complement(10389..10409)
                     /label=Fragment 2.REV
                     /note="color: black; sequence: ATAGGAACTTCACGCGGTGGC"
     primer_bind     10390..10456
                     /label=Fragment 3.FOR
                     /note="color: black; sequence: 
                     CCACCGCGTGAAGTTCCTATACTTTCTAGAGAATAGGAACTTCCCGCGGGTTTGCGCTT
                     CAAACCAC"
     misc_feature    complement(10399..10432)
                     /label=FRT
                     /note="color: #000000; direction: LEFT"
     terminator      10439..10904
                     /label=CauENO1term
                     /note="color: #ffff00; direction: RIGHT"
     primer_bind     complement(10885..10930)
                     /label=Fragment 3.REV
                     /note="color: black; sequence: 
                     CTGAGGCCTGCATGCGAGCTGAGCTCTTGAACTAGCGGGAGGGTTG"
     rep_origin      complement(11381..11969)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
                     /note="color: #ffff00"
     CDS             complement(12140..13000)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /note="color: #ffcc00"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 cccgaaaagt gccacctaaa ttgtaagcgt taatattttg ttaaaattcg cgttaaattt
       61 ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc
      121 aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga gtccactatt
      181 aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg atggcccact
      241 acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag cactaaatcg
      301 gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga acgtggcgag
      361 aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg tagcggtcac
      421 gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg cgtcccattc
      481 gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
      541 ccagctggcg aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc
      601 ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg taatacgact cactataggg
      661 cgaattgagg cctgcatgcg gtaccccagg attctacgcg cattgcaact ccgaggccga
      721 taccctttga ccagtggtca attcgtcaca attggcaaca ttttcatgat ttggcagctt
      781 cacgttgaac aattgaacca gcagaatcca gcaaaacgag tttccgttgt gaatgcccct
      841 ttgtgtgtgc aaataatcat ggtggctccc taatggcata ccctcagtca cctggaggaa
      901 ttgctcgctc gagtctcctg aggtgtcctc ctgcctgtgg aaaaagcggc caggtgtggt
      961 ccaccgttgg aacagcttgc tgtataaata ccacaggtgc gcacgcccca ggggcctttt
     1021 tttttttctc ctccctatcc aaacttaatt ttcatccccg ggatggataa aaagtatagt
     1081 attggtttag atattggtac taactctgtg ggttgggcag ttatcactga cgaatataaa
     1141 gttccatcaa agaaatttaa ggtgttaggt aacactgaca gacactcaat aaaaaagaat
     1201 cttatcggtg ctcttttgtt cgactccggt gaaactgccg aggctacacg tttaaaaaga
     1261 acagcaagaa gaagatatac ccgtagaaaa aatagaatat gttatttaca agaaatcttt
     1321 tctaatgaaa tggctaaagt tgatgattcc tttttccata gattggaaga gtcatttttg
     1381 gttgaagaag acaaaaagca tgagagacat ccaatctttg ggaatatagt tgatgaagtg
     1441 gcttaccatg aaaaatatcc taccatttat catttaagaa agaaattggt agattcaact
     1501 gataaagctg accttagatt aatctattta gcacttgccc atatgattaa atttagaggt
     1561 cattttttga ttgaaggtga tttgaaccca gataattctg acgtggataa attatttatt
     1621 caattagtcc aaacctacaa ccaattattt gaggaaaatc caattaatgc tagtggtgtc
     1681 gatgccaaag ctatattatc agccagatta tcaaaatcta gacgtttgga aaatttgatt
     1741 gcccaattgc caggagaaaa aaagaatgga ttatttggaa acttgatcgc attatcattg
     1801 ggtttgacac caaattttaa atctaatttt gatttagctg aagatgctaa attacaatta
     1861 tcaaaagaca cctatgacga cgatttggac aatttacttg ctcaaattgg tgatcaatat
     1921 gcagatttgt tcttagctgc taaaaactta tctgatgcta ttttgttgtc tgatattttg
     1981 agagtgaaca cagaaataac caaagctcca ttatcagcat ctatgatcaa acgttatgat
     2041 gaacaccatc aggatttgac tttattgaaa gctttggtga gacaacaatt gccagagaag
     2101 tataaagaaa tctttttcga tcaatctaaa aacgggtatg caggttatat tgatgggggt
     2161 gcctcccaag aggaatttta caaatttata aaacctattt tagaaaagat ggatgggact
     2221 gaggaacttt tggtcaaatt gaacagagaa gatttgttac gtaaacagag aacttttgat
     2281 aatggtagta tacctcacca aattcatttg ggtgagttgc atgcaatttt aagaagacaa
     2341 gaagattttt atccattttt aaaagataat agagaaaaaa tcgagaaaat tttaaccttt
     2401 agaattccat actatgttgg gcctttggct agaggtaatt caagatttgc ctggatgaca
     2461 cgtaaatcag aagaaactat taccccttgg aattttgaag aggttgttga taaaggagca
     2521 tcagcacaga gttttattga aagaatgacc aatttcgata aaaacttacc aaatgaaaaa
     2581 gttttaccaa aacattcctt gttatacgaa tattttactg tttacaatga acttacaaag
     2641 gttaaatatg ttactgaagg tatgcgtaag ccagcctttt tatctggaga acagaaaaag
     2701 gcaatagttg atttattgtt taaaacaaat agaaaagtta ctgttaaaca attaaaagaa
     2761 gattacttta agaaaattga atgttttgat tcagttgaaa tcagtggtgt tgaagacaga
     2821 tttaatgcta gtttaggaac ttaccatgat ttacttaaaa ttatcaaaga taaagatttc
     2881 ttggataacg aagaaaatga agacatttta gaagacattg ttttaacctt aactttattc
     2941 gaagatagag agatgattga agaacgtttg aagacttatg cacatttgtt tgacgataaa
     3001 gtgatgaaac agttgaaaag aagacgttat actggatggg gtagattgtc tcgtaaattg
     3061 atcaatggaa ttagagataa acaaagtggt aaaactatct tggacttttt gaaatctgac
     3121 ggatttgcta atagaaattt catgcaattg atccacgacg atagtttgac atttaaagaa
     3181 gacatccaaa aggcccaagt gagtgggcaa ggtgattcat tacatgaaca tattgcaaat
     3241 ttagccggat ctcctgctat taagaaaggg atattacaaa ctgttaaagt tgtggatgaa
     3301 ttagtgaaag taatgggaag acataaacct gaaaacattg tcattgagat ggcaagagaa
     3361 aatcaaacta cacaaaaagg acagaaaaat agtagagaac gtatgaaaag aatagaagag
     3421 ggtattaaag aattgggtag tcaaatattg aaagaacacc cagtggaaaa tacccagttg
     3481 caaaatgaaa aattatatct ttactacctt caaaatggac gtgatatgta tgttgatcag
     3541 gaattagata taaatagact ttcagattat gatgtagatc atatagttcc acaatctttc
     3601 ttgaaagatg attccataga caataaagta ttaactagaa gtgataaaaa tagaggtaaa
     3661 agtgataatg tcccaagtga ggaagtcgtc aaaaagatga aaaattactg gcgtcaactt
     3721 ttgaatgcta aattaattac tcaaagaaaa tttgataatt tgactaaagc agaaagaggt
     3781 gggctttctg aattagataa agccgggttc attaaaagac aattggtcga aactagacaa
     3841 attactaaac atgttgccca aattttagat tcccgtatga acactaagta tgacgaaaat
     3901 gataagttaa tacgtgaggt taaagtcatt actttaaaat caaaacttgt ctctgatttc
     3961 agaaaggatt tccaattcta taaagttaga gaaattaata attatcatca tgctcatgat
     4021 gcatatttga atgctgtagt tggaactgct ttaatcaaga aataccctaa attagaatct
     4081 gaatttgtat atggtgatta caaagtctat gatgttagaa agatgattgc taaatcagaa
     4141 caagaaattg gtaaagctac agctaaatac ttcttttact ctaacattat gaatttcttt
     4201 aaaacagaaa ttactttggc aaacggtgaa attagaaaaa gacctcttat tgaaacaaat
     4261 ggtgagactg gagagatagt ttgggacaaa gggcgtgatt tcgctactgt tagaaaagtt
     4321 ttatcaatgc cacaagttaa cattgtaaag aaaacagagg ttcaaactgg tggtttctca
     4381 aaagaaagta ttttgcctaa aagaaatagt gataaattga ttgccagaaa aaaggattgg
     4441 gatccaaaga aatatggtgg tttcgactca ccaaccgtag cctattctgt tttggttgtg
     4501 gcaaaggttg aaaagggtaa aagtaaaaag cttaaatcag taaaagaact tttgggtatt
     4561 acaataatgg aaagaagttc ctttgaaaag aaccctattg attttttgga agctaaaggt
     4621 tataaggaag taaagaagga cttaataatc aaattgccta aatattcttt atttgaatta
     4681 gaaaatggga gaaaaagaat gttggcttct gctggagaat tgcaaaaggg taatgaatta
     4741 gcattgcctt ccaaatatgt taacttcttg tatttagctt cacactatga aaagttgaaa
     4801 gggtcaccag aagataacga gcaaaaacaa ttatttgttg aacaacacaa acactactta
     4861 gatgagatta tagaacaaat tagtgaattc agtaaaagag tgatattagc tgatgcaaat
     4921 ttagataaag ttttgtcagc ctataacaaa catagagata agccaattag agaacaagca
     4981 gaaaacatta ttcacttatt tacccttacc aatttaggag cacctgctgc tttcaagtat
     5041 tttgatacaa caattgatcg taaaagatat acctcaacaa aagaagtctt agacgccacc
     5101 ttaattcatc aatcaatcac tggattgtat gagacaagaa ttgatttgtc tcaattgggt
     5161 ggtgatgaag gggctgatcc taagaagaaa agaaaagttg atccaaagaa aaagcgtaag
     5221 gtggatccta agaaaaagag aaaggttgac tacaaagacc atgacggtga ttataaagat
     5281 catgacatcg actacaagga tgacgatgac aagtgataat gactgcagag atccatcgac
     5341 ctgccgccaa gctaattccg ggcgaatttc tgtcgagtca tgtaattagt tatgtcacgc
     5401 ttacattcac gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct
     5461 gaagtctagg tccctattta tttttttata gttatgttag tattaagaac gttatttata
     5521 tttcaaattt ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa
     5581 accttgcttg agaaggtttt gggacgctcg aaggctttaa tttgcggccg ggccccccct
     5641 cgaggaagtt cctatacttt ctagagaata ggaacttcgg atccactagt tctagatttt
     5701 tgcaagcatt taaatattgc caagtaaaaa cttcaaattt tctttcccct tggaactttg
     5761 actttatttt tttgacagat tattttgaca cacacacacc aaatgtgtta ccccttaaaa
     5821 caaaaaaaca cttttttaca atttcttggt atccagaatc attctaagca tcattcaatt
     5881 ataatttcaa tccaaaaaag tagttttagt ttgacttgaa acgtcaacaa acacaaattt
     5941 caaatcataa cctctcctgt tgcctgtcaa caacacacca taaggagaag gaataggagg
     6001 aggaggagat agaaacttgc acggcaccac aaaacacaaa attgatttca accaatacgg
     6061 tgacaacaac aatagatttc cgatagaaat aatgattatc ggaataagct agctttgctt
     6121 tgctttgctt tgctttttga cttgctctaa tttttcgaaa ataataatgg agaaaagttc
     6181 aaggtgttta atgcatcaac taaaacagaa aataatacat tagactaaac ttttaatctt
     6241 tctagtacca ataattcacg cgtgcgtttt aatcccaatc atgaaatgaa gaagttattt
     6301 ccctttttct ttcatcaaaa aagaactaaa ttatttttta aattttagta aacaaaacct
     6361 ggaaatcggg gaaaccgggg gaggggggca gaaggtgaaa cgggtaatat tgataaattt
     6421 aatctataat tgataaagtt aaatttaaat tgatttgaat tgatttgaat tgaatgaaat
     6481 gcatttgaat aaacggcatc aaactaaaaa aatatagatc acattcatag taaaacgata
     6541 acaaagaaca ccacaattta tagcaatgat aataaacatc taaaaagaaa agggtacgag
     6601 aaggagaatg aaaaaaaaca ataagctagt tcttaatctg ttcagatatc taatttcaaa
     6661 aaaaagaata gtataaaagg atagttgatt cctcttggtt gttgaaaatt tgaatgatat
     6721 caatcaatta atcaatcaaa taacaacaac ccactagaca tcaccattgt cgacatgcca
     6781 caatttgata tattatgtaa aacaccacct aaggtgcttg ttcgtcagtt tgtggaaagg
     6841 tttgaaagac cttcaggtga gaaaatagca ttatgtgctg ctgaactaac ctatttatgt
     6901 tggatgatta cacataacgg aacagcaatc aagagagcca cattcatgag ctataatact
     6961 atcataagca attcgttgag tttcgatatt gtcaataaat cactccagtt taaatacaag
     7021 acgcaaaaag caacaatttt ggaagcctca ttaaagaaat tgattcctgc ttgggaattt
     7081 acaattattc cttactatgg acaaaaacat caatctgata tcactgatat tgtaagtagt
     7141 ttgcaattac agttcgaatc atcggaagaa gcagataagg ggaatagcca cagtaaaaaa
     7201 atgcttaaag cacttctaag tgagggtgaa agcatctggg agatcactga gaaaatacta
     7261 aattcgtttg agtatacttc gagatttaca aaaacaaaaa ctttatacca attcctcttc
     7321 ctagctactt tcatcaactg tggaagattc agcgatatta agaacgttga tccgaaatca
     7381 tttaaattag tccaaaataa gtatttggga gtaataatcc agtgtttagt gacagagaca
     7441 aagacaagcg ttagtaggca catatacttc tttagcgcaa ggggtaggat cgatccactt
     7501 gtatatttgg atgaattttt gaggaattct gaaccagtcc taaaacgagt aaataggacc
     7561 ggcaattctt caagcaataa acaggaatac caattattaa aagataactt agtcagatcg
     7621 tacaataaag ctttgaagaa aaatgcgcct tattcaatct ttgctataaa aaatggccca
     7681 aaatctcaca ttggaagaca tttgatgacc tcatttcttt caatgaaggg cctaacggag
     7741 ttgactaatg ttgtgggaaa ttggagcgat aagcgtgctt ctgccgtggc caggacaacg
     7801 tatactcatc agataacagc aatacctgat cactacttcg cactagtttc tcggtactat
     7861 gcatatgatc caatatcaaa ggaaatgata gcattgaagg atgagactaa tccaattgag
     7921 gagtggcagc atatagaaca gctaaagggt agtgctgaag gaagcatacg ataccccgca
     7981 tggaatggga taatatcaca ggaggtacta gactaccttt catcctacat aaatagacgc
     8041 atataagagt gaaattctgg aaatctggaa atctggtttt gtattcttgt tattcttctt
     8101 tttgttatta catatataac ttgttacttt tttaaaaaaa tctttgtata ttttataaat
     8161 atataaaact aaatttaaga aaaagagaaa aatgttttat ttgagagatt gaaattttac
     8221 ttgaatttag cttagctttt ataaagtatt attatgtaaa aaaacaaaac aaatatacat
     8281 caaaaagtta agactataaa atagccaccc aaggcatttc tatatcttgt tgttgttgtt
     8341 ttcatcttct gtatcagagg aacttatttt attattttcg tcacgggtat tttctcttgt
     8401 ttgatgattc atcccattca ttccatcata aaatgtcgac actggatggc ggcgttagta
     8461 tcgaatcgac agcagtatag cgaccagcat tcacatacga ttgacgcatg atattacttt
     8521 ctgcgcactt aacttcgcat ctgggcagat gatgtcgagg cgaaaaaaaa tataaatcac
     8581 gctaacattt gattaaaata gaacaactac aatataaaaa aactatacaa atgacaagtt
     8641 cttgaaaaca agaatctttt tattgtcagt actgactcga gttattatgg acatggcata
     8701 gacatataca aagcttgttc accatcggaa gcagtaccat cgtataaagc agtatccaaa
     8761 ccacacaaag tgaaacccat tcttctataa gcatgaatag ctggagcatt aacattggta
     8821 acttccaacc acaaatgacc agcacctctt tctctggcga attcagtagc caaacccatc
     8881 aaagctctac caacaccatg acctctatgt tctggagcaa cttcaatatc ttcaacagtc
     8941 aatcttctgt tccaaccaga ataagaaaca acaacgaaac cagccaaatc accatcatca
     9001 ccataagcaa cgaaagttct agaatctgga tcaccatctt caccagcatc ggattcatca
     9061 tcggattcat catctgggaa aaccttagtc aatggtggat caactggaac ttctctcaaa
     9121 gtgaaaccat caccagtagc agtaactcta aaaacagtat cggtagtgaa agaaccatcc
     9181 aaagcttcaa tagcttcagc atcacctgga acagaagttc tgtatctata agcagtatca
     9241 tccaaagtag tagacataat tgtaggatcc ggttgtttat gttcggatgt gatgtgagaa
     9301 ctgtatccta gcaagatttt aaaaggaagt atatgaaaga agaacctcag tggcaaatcc
     9361 taacctttta tatttctcta caggggcgcg gcgtggggac aattcaacgc gtctgtgagg
     9421 ggagcgtttc cctgctcgca ggtctgcagc gaggagccgt aatttttgct tcgcgccgtg
     9481 cggccatcaa aatgtatgga tgcaaatgat tatacatggg gatgtatggg ctaaatgtac
     9541 gggcgacagt cacatcatgc ccctgagctg cgcacgtcaa gactgtcaag gagggtattc
     9601 tgggcctcca tgtcgctggc cgggtgaccc ggcggggacg aggcaagctt gatgtgcggc
     9661 cgcacagact caatcaacga agtttttttt tttttttttt tttttttttt cttcttcata
     9721 gacaataacc ttatcaaagt ttataaaact atatcgcaat agaaaacaaa cccccaatca
     9781 gcttaacttt tagagtcact taccctacaa aggtgtaagc ttccctcaga tcaggcggca
     9841 cgcatctgat atctaacatc cattgtaaaa ttagtgaaga ttaacactca gagccagtta
     9901 ttttgaacac agatggcttt tggctgctct tatcgtctac gtttcttttt tttttttttt
     9961 tttttttttt tttttttttc ctaatttttt acgagttcac taattggttt cgggggctca
    10021 attttcgaat gcgacattca taaactttga ccttttatgt gttgatgcgg ataattttga
    10081 cctcaaaact cggaaaaatt gatgtatagc gtattcgaca tggctttaaa agtcgcagag
    10141 ttctacgatt ttcatttgca ctggcccatc cggtaagcct cggggctccg gcatgtgagg
    10201 gttcgactcc ctcagcagaa aacaggagac ggaattccgt ctcgttttag agctagaaat
    10261 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
    10321 ttttttctcg agttttttta tcgagtgttt aaggataatg ataactgaag agaagaatta
    10381 gttttgccgc caccgcgtga agttcctata ctttctagag aataggaact tcccgcgggt
    10441 ttgcgcttca aaccactttg gtatataatc tagacgaaat atcatactaa gaacgttaca
    10501 atgcagtaga tatgtacaag actagcttct cgaaagctca tctaaagcct caactattct
    10561 ctttaccacg actctcacat cctcaagagt attgtacaat ggtgtgggtg caatacgaat
    10621 aacatcgggt ctcctctcgt cgcaaatgat tgcatgcttg tgtaagtact cgtttacctg
    10681 ttccataaca ttcttgcttc tgtcgtcatc atgcggttgg aataacactg aaagttgaca
    10741 tcctctctca ctttcttcac atggagtcaa gatcttgaat cccaacagtg cattgtccag
    10801 cggatcttcg atgtagtatt tgctgccctt caaaagcgtt agcaagcagc cagtcaaaga
    10861 aacactagtt tttctaagat tgtccaaccc tcccgctagt tcaagagctc agctcgcatg
    10921 caggcctcag cttttgttcc ctttagtgag ggttaattgc gcgcttggcg taatcatggt
    10981 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
    11041 ggagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
    11101 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
    11161 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
    11221 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
    11281 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
    11341 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
    11401 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
    11461 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
    11521 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
    11581 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
    11641 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
    11701 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
    11761 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
    11821 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
    11881 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
    11941 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
    12001 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
    12061 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
    12121 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
    12181 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
    12241 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc
    12301 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
    12361 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
    12421 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
    12481 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
    12541 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
    12601 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
    12661 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
    12721 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
    12781 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
    12841 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
    12901 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
    12961 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
    13021 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
    13081 aaaataaaca aataggggtt ccgcgcacat ttc
//
