LOCUS       pAK011                  4553 bp ds-DNA     circular     09-MAY-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     terminator      24..129
                     /label="T0"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          117..134
                     /label="oRC040_correct"
                     /note="sequence: caggagtccaagcgagct"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     primer          complement(117..134)
                     /label="oRC053"
                     /note="sequence: agctcgcttggactcctg"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(159..1014)
                     /label="Amp"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     primer          1090..1120
                     /label="oCK1051"
                     /note="sequence: TTAGAAAAATAAACAAATAGGGGTTCCGCGC"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          1099..1118
                     /label="oCDB021"
                     /note="sequence: TAAACAAATAGGGGTTCCGC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          1114..1145
                     /label="oRC031"
                     /note="sequence: tccgcgcacatttccccgaaaagtgccacctg"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          complement(1123..1145)
                     /label="oCK348"
                     /note="sequence: ccaggtggcacttttcggggaaat"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     primer          1146..1170
                     /label="oAK009"
                     /note="sequence: TGGCAATTCCGACGTCGCCTACGGT"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     misc_feature    1147..1166
                     /label="J133"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    1157..1176
                     /label="J131"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1157..1176)
                     /label="J132*"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1162..1331
                     /label="J1 upstream sequence"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(1166..1185)
                     /label="J130"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1167..1186
                     /label="J129"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1176..1195)
                     /label="J128"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1177..1196
                     /label="J127"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1186..1205)
                     /label="J126"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1187..1206
                     /label="J125"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1196..1215)
                     /label="J124"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1197..1216
                     /label="J123"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1206..1225)
                     /label="J122"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1207..1226
                     /label="J121"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1216..1235)
                     /label="J120"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1217..1236
                     /label="J119"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1226..1245)
                     /label="J118"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1227..1246
                     /label="J117"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1236..1255)
                     /label="J116"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1237..1256
                     /label="J115"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1246..1265)
                     /label="J114"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1247..1266
                     /label="J113"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1256..1275)
                     /label="J112"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1257..1276
                     /label="J111"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1266..1285)
                     /label="J110"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1267..1286
                     /label="J109"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(1276..1295)
                     /label="J108"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1277..1296
                     /label="J107"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(1286..1305)
                     /label="J106"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    1287..1306
                     /label="J105"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1296..1315)
                     /label="J104"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1297..1316
                     /label="J103"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(1306..1325)
                     /label="J102"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    1307..1326
                     /label="J101"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1312..1356
                     /label="oAK007"
                     /note="sequence: TCCACCGGATACCTCCGGACGTCATAATCATCGCTACGAGCTGTG"
                     /ApEinfo_revcolor="#d6b295"
                     /ApEinfo_fwdcolor="#d6b295"
     primer          complement(1314..1331)
                     /label="oAK006"
                     /note="sequence: GTCCGGAGGTATCCGGTG"
                     /ApEinfo_revcolor="#f7977a"
                     /ApEinfo_fwdcolor="#f7977a"
     misc_feature    1332..1453
                     /label="J3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     primer          1334..1381
                     /label="oCK1230"
                     /note="sequence: TATTAAGAGACAATGCGCTCGTGAAAGATGCATAAAGCTCGTACGACG"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     primer          1334..1381
                     /label="oCK1229"
                     /note="sequence: TCCACCGGATACCTCCGGACGTGAAAGATGCATAAAGCTCGTACGACG"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     misc_feature    1389..1408
                     /label="J309"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(1398..1417)
                     /label="J308"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    1399..1418
                     /label="J307"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(1408..1427)
                     /label="J306"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        1454..1488
                     /label="BBa_J23117"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1489..1515
                     /label="Bujard RBS (strong)"
                     /ApEinfo_revcolor="#db7aff"
                     /ApEinfo_fwdcolor="#db7aff"
     primer          1489..1515
                     /label="oAK005"
                     /note="sequence: GAATTCATTAAAGAGGAGAAAGGTACC"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     primer          complement(1489..1515)
                     /label="oAK008"
                     /note="sequence: GGTACCTTTCTCCTCTTTAATGAATTC"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     misc_feature    1516..2193
                     /label="mRFP"
                     /ApEinfo_revcolor="#ff3737"
                     /ApEinfo_fwdcolor="#ff3737"
     primer          complement(1680..1699)
                     /label="oCD030"
                     /note="sequence: ACAGGATGTCCCAAGCGAAC"
                     /ApEinfo_revcolor="#9eafd2"
                     /ApEinfo_fwdcolor="#9eafd2"
     misc_feature    2218..2346
                     /label="dbl term"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      complement(2348..23)
                     /label="pSC101** ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
ORIGIN
        1 ttacccgtct tactgtccct agtgcttgga ttctcaccaa taaaaaacgc ccggcggcaa
       61 ccgagcgttc tgaacaaatc cagatggagt tctgaggtca ttactggatc tatcaacagg
      121 agtccaagcg agctcgtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
      181 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
      241 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
      301 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
      361 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
      421 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
      481 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
      541 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
      601 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
      661 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
      721 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
      781 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
      841 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
      901 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
      961 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1021 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1081 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1141 acctgTGGCA ATTCCGACGT CGCCTACGGT ATCCACCGGA GACCTATGGC AGCCTCCGGC
     1201 CGCCATAGGA CACCTTTGGT TGCCAAGGGT GACCTATGGT GACCATGGGC CACCACGGGC
     1261 GACCTCAGGT ATCCTGCGGT GTCCTGCGGT TACCAAAGGC GTCCTTTGGG TTCCACCGGA
     1321 TACCTCCGGA CGTCATAATC ATCGCTACGA GCTGTGAAAG ATGCATAAAG CTCGTACGAC
     1381 GCGTTCGCTC GTCTCCTCAC TTCTCCTACG GAGCGTTCTG GACACAACGT CGTCTTGAAG
     1441 TTGCGATTAT AGAttgacag ctagctcagt cctagggatt gtgctagcGA ATTCATTAAA
     1501 GAGGAGAAAG GTACCATGGC GAGTAGCGAA GACGTTATCA AAGAGTTCAT gcgtttcaaa
     1561 gttcgtatgg aaggttccgt taacggtcac gagttcgaaa tcgaaggtga aggtgaaggt
     1621 cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta ccaaaggtgg tccgctgccg
     1681 ttcgcttggg acatcctgtc cccgcagttc cagtacggtt ccaaagctta cgttaaacac
     1741 ccggctgaca tcccggacta cctgaaactg tccttcccgg aaggtttcaa atgggaacgt
     1801 gttatgaact tcgaagacgg tggtgttgtt accgttaccc aggactcctc cctgcaagac
     1861 ggtgagttca tctacaaagt taaactgcgt ggtaccaact tcccgtccga cggtccggtt
     1921 atgcagaaaa aaaccatggg ttgggaagct tccaccgaac gtatgtaccc ggaagacggt
     1981 gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag acggtggtca ctacgacgct
     2041 gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc tgccgggtgc ttacaaaacc
     2101 gacatcaaac tggacatcac ctcccacaac gaagactaca ccatcgttga acagtacgaa
     2161 cgtgctgaag gtcgtcactc caccggtgct taaggatcca aactcgagta aggatctCCA
     2221 GGCATCAAAT AAAACGAAAG GCTCAGTCGA AAGACTGGGC CTTTCGTTTT ATCTGTTGTT
     2281 TGTCGGTGAA CGCTCTCTAC TAGAGTCACA CTGGCTCACC TTCGGGTGGG CCTTTCTGCG
     2341 TTTATACcta gggtacgggt tttgctgccc gcaaacgggc tgttctggtg ttgctagttt
     2401 gttatcagaa tcgcagatcc ggcttcagcc ggtttgccgg ctgaaagcgc tatttcttcc
     2461 agaattgcca tgattttttc cccacgggag gcgtcactgg ctcccgtgtt gtcggcagct
     2521 ttgattcgat aagcagcatc gcctgtttca ggctgtctat gtgtgactgt tgagctgtaa
     2581 caagttgtct caggtgttca atttcatgtt ctagttgctt tgttttactg gtttcacctg
     2641 ttctattagg tgttacatgc tgttcatctg ttacattgtc gatctgttca tggtgaacag
     2701 ctttgaatgc accaaaaact cgtaaaagct ctgatgtatc tatctttttt acaccgtttt
     2761 catctgtgca tatggacagt tttccctttg atatgtaacg gtgaacagtt gttctacttt
     2821 tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc tcagatcctt
     2881 ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag ccatgagaac
     2941 gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc aaaactggtg
     3001 agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt atgtaggtag
     3061 gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg gttgttctca
     3121 agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa cgtatcagtc
     3181 gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa atctttactt
     3241 attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt ttcaagcatt
     3301 aacatgaact taaattcatc aaggctaatc tctatatttg ccttgtgagt tttcttttgt
     3361 gttagttctt ttaataacca ctcataaatc ctcatagagt atttgttttc aaaagactta
     3421 acatgttcca gattatattt tatgaatttt tttaactgga aaagataagg caatatctct
     3481 tcactaaaaa ctaattctaa tttttcgctt gagaacttgg catagtttgt ccactggaaa
     3541 atctcaaagc ctttaaccaa aggattcctg atttccacag ttctcgtcat cagctctctg
     3601 gttgctttag ctaatacacc ataagcattt tccctactga tgttcatcat ctgagcgtat
     3661 tggttataag tgaacgatac cgtccgttct ttccttgtag ggttttcaat cgtggggttg
     3721 agtagtgcca cacagcataa aattagcttg gtttcatgct ccgttaagtc atagcgacta
     3781 atcgctagtt catttgcttt gaaaacaact aattcagaca tacatctcaa ttggtctagg
     3841 tgattttaat cactatacca attgagatgg gctagtcaat gataattact agtccttttc
     3901 ccgggtgatc tgggtatctg taaattctgc tagacctttg ctggaaaact tgtaaattct
     3961 gctagaccct ctgtaaattc cgctagacct ttgtgtgttt tttttgttta tattcaagtg
     4021 gttataattt atagaataaa gaaagaataa aaaaagataa aaagaataga tcccagccct
     4081 gtgtataact cactacttta gtcagttccg cagtattaca aaaggatgtc gcaaacgctg
     4141 tttgctcctc tacaaaacag accttaaaac cctaaaggct taagtagcac cctcgcaagc
     4201 tcgggcaaat cgctgaatat tccttttgtc tccgaccatc aggcacctga gtcgctgtct
     4261 ttttcgtgac attcagttcg ctgcgctcac ggctctggca gtgaatgggg gtaaatggca
     4321 ctacaggcgc cttttatgga ttcatgcaag gaaactaccc ataatacaag aaaagcccgt
     4381 cacgggcttc tcagggcgtt ttatggcggg tctgctatgt ggtgctatct gactttttgc
     4441 tgttcagcag ttcctgccct ctgattttcc agtctgacca cttcggatta tcccgtgaca
     4501 ggtcattcag actggctaat gcacccagta aggcagcggt atcatcaaca ggc
//