LOCUS       pKO023                  4556 bp ds-DNA     circular     09-MAY-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     misc_feature    127..146
                     /label="J133"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    137..156
                     /label="J131"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(137..156)
                     /label="J132*"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    142..311
                     /label="J1 upstream sequence"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(146..165)
                     /label="J130"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    147..166
                     /label="J129"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(156..175)
                     /label="J128"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    157..176
                     /label="J127"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(166..185)
                     /label="J126"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    167..186
                     /label="J125"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(176..195)
                     /label="J124"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    177..196
                     /label="J123"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(186..205)
                     /label="J122"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    187..206
                     /label="J121"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(196..215)
                     /label="J120"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    197..216
                     /label="J119"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(206..225)
                     /label="J118"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    207..226
                     /label="J117"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(216..235)
                     /label="J116"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    217..236
                     /label="J115"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(226..245)
                     /label="J114"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    227..246
                     /label="J113"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(236..255)
                     /label="J112"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    237..256
                     /label="J111"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(246..265)
                     /label="J110"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    247..266
                     /label="J109"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(256..275)
                     /label="J108"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    257..276
                     /label="J107"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(266..285)
                     /label="J106"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    267..286
                     /label="J105"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(276..295)
                     /label="J104"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    277..296
                     /label="J103"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(286..305)
                     /label="J102"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    287..306
                     /label="J101"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    312..382
                     /label="J3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    369..382
                     /label="J309"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(378..382)
                     /label="J308"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    379..382
                     /label="J307"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    389..391
                     /label="J309"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(389..400)
                     /label="J308"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     misc_feature    389..401
                     /label="J307"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    389..436
                     /label="J3"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    complement(391..410)
                     /label="J306"
                     /ApEinfo_revcolor="#75c6a9"
                     /ApEinfo_fwdcolor="#75c6a9"
     promoter        437..471
                     /label="BBa_J23117"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    472..498
                     /label="Bujard RBS (strong)"
                     /ApEinfo_revcolor="#db7aff"
                     /ApEinfo_fwdcolor="#db7aff"
     misc_feature    499..1176
                     /label="mRFP"
                     /ApEinfo_revcolor="#ff3737"
                     /ApEinfo_fwdcolor="#ff3737"
     misc_feature    1201..1329
                     /label="dbl term"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      complement(1331..3559)
                     /label="pSC101** ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     terminator      3560..3665
                     /label="T0"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(3695..4550)
                     /label="Amp"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
       61 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
      121 acctgTGGCA ATTCCGACGT CGCCTACGGT ATCCACCGGA GACCTATGGC AGCCTCCGGC
      181 CGCCATAGGA CACCTTTGGT TGCCAAGGGT GACCTATGGT GACCATGGGC CACCACGGGC
      241 GACCTCAGGT ATCCTGCGGT GTCCTGCGGT TACCAAAGGC GTCCTTTGGG TTCCACCGGA
      301 TACCTCCGGA CGTCATAATC ATCGCTACGA GCTGTGAAAG ATGCATAAAG CTCGTACGAC
      361 GCGTTCGCTC GTCTCCTCAC TTCATTCTCA ACGGAGCGTT CTGGACACAA CGTCGTCTTG
      421 AAGTTGCGAT TATAGAttga cagctagctc agtcctaggg attgtgctag cGAATTCATT
      481 AAAGAGGAGA AAGGTACCAT GGCGAGTAGC GAAGACGTTA TCAAAGAGTT CATgcgtttc
      541 aaagttcgta tggaaggttc cgttaacggt cacgagttcg aaatcgaagg tgaaggtgaa
      601 ggtcgtccgt acgaaggtac ccagaccgct aaactgaaag ttaccaaagg tggtccgctg
      661 ccgttcgctt gggacatcct gtccccgcag ttccagtacg gttccaaagc ttacgttaaa
      721 cacccggctg acatcccgga ctacctgaaa ctgtccttcc cggaaggttt caaatgggaa
      781 cgtgttatga acttcgaaga cggtggtgtt gttaccgtta cccaggactc ctccctgcaa
      841 gacggtgagt tcatctacaa agttaaactg cgtggtacca acttcccgtc cgacggtccg
      901 gttatgcaga aaaaaaccat gggttgggaa gcttccaccg aacgtatgta cccggaagac
      961 ggtgctctga aaggtgaaat caaaatgcgt ctgaaactga aagacggtgg tcactacgac
     1021 gctgaagtta aaaccaccta catggctaaa aaaccggttc agctgccggg tgcttacaaa
     1081 accgacatca aactggacat cacctcccac aacgaagact acaccatcgt tgaacagtac
     1141 gaacgtgctg aaggtcgtca ctccaccggt gcttaaggat ccaaactcga gtaaggatct
     1201 CCAGGCATCA AATAAAACGA AAGGCTCAGT CGAAAGACTG GGCCTTTCGT TTTATCTGTT
     1261 GTTTGTCGGT GAACGCTCTC TACTAGAGTC ACACTGGCTC ACCTTCGGGT GGGCCTTTCT
     1321 GCGTTTATAC ctagggtacg ggttttgctg cccgcaaacg ggctgttctg gtgttgctag
     1381 tttgttatca gaatcgcaga tccggcttca gccggtttgc cggctgaaag cgctatttct
     1441 tccagaattg ccatgatttt ttccccacgg gaggcgtcac tggctcccgt gttgtcggca
     1501 gctttgattc gataagcagc atcgcctgtt tcaggctgtc tatgtgtgac tgttgagctg
     1561 taacaagttg tctcaggtgt tcaatttcat gttctagttg ctttgtttta ctggtttcac
     1621 ctgttctatt aggtgttaca tgctgttcat ctgttacatt gtcgatctgt tcatggtgaa
     1681 cagctttgaa tgcaccaaaa actcgtaaaa gctctgatgt atctatcttt tttacaccgt
     1741 tttcatctgt gcatatggac agttttccct ttgatatgta acggtgaaca gttgttctac
     1801 ttttgtttgt tagtcttgat gcttcactga tagatacaag agccataaga acctcagatc
     1861 cttccgtatt tagccagtat gttctctagt gtggttcgtt gtttttgcgt gagccatgag
     1921 aacgaaccat tgagatcata cttactttgc atgtcactca aaaattttgc ctcaaaactg
     1981 gtgagctgaa tttttgcagt taaagcatcg tgtagtgttt ttcttagtcc gttatgtagg
     2041 taggaatctg atgtaatggt tgttggtatt ttgtcaccat tcatttttat ctggttgttc
     2101 tcaagttcgg ttacgagatc catttgtcta tctagttcaa cttggaaaat caacgtatca
     2161 gtcgggcggc ctcgcttatc aaccaccaat ttcatattgc tgtaagtgtt taaatcttta
     2221 cttattggtt tcaaaaccca ttggttaagc cttttaaact catggtagtt attttcaagc
     2281 attaacatga acttaaattc atcaaggcta atctctatat ttgccttgtg agttttcttt
     2341 tgtgttagtt cttttaataa ccactcataa atcctcatag agtatttgtt ttcaaaagac
     2401 ttaacatgtt ccagattata ttttatgaat ttttttaact ggaaaagata aggcaatatc
     2461 tcttcactaa aaactaattc taatttttcg cttgagaact tggcatagtt tgtccactgg
     2521 aaaatctcaa agcctttaac caaaggattc ctgatttcca cagttctcgt catcagctct
     2581 ctggttgctt tagctaatac accataagca ttttccctac tgatgttcat catctgagcg
     2641 tattggttat aagtgaacga taccgtccgt tctttccttg tagggttttc aatcgtgggg
     2701 ttgagtagtg ccacacagca taaaattagc ttggtttcat gctccgttaa gtcatagcga
     2761 ctaatcgcta gttcatttgc tttgaaaaca actaattcag acatacatct caattggtct
     2821 aggtgatttt aatcactata ccaattgaga tgggctagtc aatgataatt actagtcctt
     2881 ttcccgggtg atctgggtat ctgtaaattc tgctagacct ttgctggaaa acttgtaaat
     2941 tctgctagac cctctgtaaa ttccgctaga cctttgtgtg ttttttttgt ttatattcaa
     3001 gtggttataa tttatagaat aaagaaagaa taaaaaaaga taaaaagaat agatcccagc
     3061 cctgtgtata actcactact ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg
     3121 ctgtttgctc ctctacaaaa cagaccttaa aaccctaaag gcttaagtag caccctcgca
     3181 agctcgggca aatcgctgaa tattcctttt gtctccgacc atcaggcacc tgagtcgctg
     3241 tctttttcgt gacattcagt tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg
     3301 gcactacagg cgccttttat ggattcatgc aaggaaacta cccataatac aagaaaagcc
     3361 cgtcacgggc ttctcagggc gttttatggc gggtctgcta tgtggtgcta tctgactttt
     3421 tgctgttcag cagttcctgc cctctgattt tccagtctga ccacttcgga ttatcccgtg
     3481 acaggtcatt cagactggct aatgcaccca gtaaggcagc ggtatcatca acaggcttac
     3541 ccgtcttact gtccctagtg cttggattct caccaataaa aaacgcccgg cggcaaccga
     3601 gcgttctgaa caaatccaga tggagttctg aggtcattac tggatctatc aacaggagtc
     3661 caagcgagct cgtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     3721 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     3781 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     3841 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     3901 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     3961 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     4021 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     4081 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     4141 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     4201 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     4261 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     4321 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     4381 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     4441 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     4501 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actctt
//