LOCUS       pCK161.06               5131 bp ds-DNA     circular     09-MAY-2025
DEFINITION  .
FEATURES             Location/Qualifiers
     promoter        148..182
                     /label="J23119 (SpeI) promoter"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     None            183..202
                     /label="J106"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    203..244
                     /label="JAD designed chimera RNA 3'UTR"
                     /ApEinfo_revcolor="#84b0dc"
                     /ApEinfo_fwdcolor="#84b0dc"
     misc_feature    249..263
                     /label="Streptococcus pyogenes tracrRNA terminator"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    264..265
                     /label="Left stem extension"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    264..286
                     /label="MS2 with 2bp stem extension"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    285..286
                     /label="Right stem extension"
                     /ApEinfo_revcolor="#b4abac"
                     /ApEinfo_fwdcolor="#b4abac"
     misc_feature    287..293
                     /label="SUP4 terminator"
                     /ApEinfo_revcolor="#008080"
                     /ApEinfo_fwdcolor="#008080"
     terminator      294..661
                     /label="TrrnB"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    425..442
                     /label="pBad_r"
                     /ApEinfo_revcolor="#faac61"
                     /ApEinfo_fwdcolor="#faac61"
     terminator      587..654
                     /label="sglTerm"
                     /ApEinfo_revcolor="#800040"
                     /ApEinfo_fwdcolor="#800040"
     misc_feature    847..862
                     /label="J131"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(847..862)
                     /label="J132*"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    848..1017
                     /label="J1 upstream sequence"
                     /ApEinfo_revcolor="#b7e6d7"
                     /ApEinfo_fwdcolor="#b7e6d7"
     misc_feature    complement(852..871)
                     /label="J130"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    853..872
                     /label="J129"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(862..881)
                     /label="J128"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    863..882
                     /label="J127"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(872..891)
                     /label="J126"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    873..892
                     /label="J125"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(882..901)
                     /label="J124"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    883..902
                     /label="J123"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(892..911)
                     /label="J122"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    893..912
                     /label="J121"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(902..921)
                     /label="J120"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    903..922
                     /label="J119"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(912..931)
                     /label="J118"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    913..932
                     /label="J117"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(922..941)
                     /label="J116"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    923..942
                     /label="J115"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(932..951)
                     /label="J114"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    933..952
                     /label="J113"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(942..961)
                     /label="J112"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    943..962
                     /label="J111"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(952..971)
                     /label="J110"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    953..972
                     /label="J109"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(962..981)
                     /label="J108"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    963..982
                     /label="J107"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    complement(972..991)
                     /label="J106"
                     /ApEinfo_revcolor="#b1ff67"
                     /ApEinfo_fwdcolor="#b1ff67"
     misc_feature    973..992
                     /label="J105"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(982..1001)
                     /label="J104"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    983..1002
                     /label="J103"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     misc_feature    complement(992..1011)
                     /label="J102"
                     /ApEinfo_revcolor="#85dae9"
                     /ApEinfo_fwdcolor="#85dae9"
     misc_feature    993..1012
                     /label="J101"
                     /ApEinfo_revcolor="#f58a5e"
                     /ApEinfo_fwdcolor="#f58a5e"
     promoter        1018..1052
                     /label="BBa_J23117"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    1053..1079
                     /label="Bujard RBS (strong)"
                     /ApEinfo_revcolor="#db7aff"
                     /ApEinfo_fwdcolor="#db7aff"
     misc_feature    1080..1757
                     /label="mRFP"
                     /ApEinfo_revcolor="#ff3737"
                     /ApEinfo_fwdcolor="#ff3737"
     misc_feature    1782..1910
                     /label="dbl term"
                     /ApEinfo_revcolor="#ff9ccd"
                     /ApEinfo_fwdcolor="#ff9ccd"
     rep_origin      complement(1912..3179)
                     /label="pSC101** ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     rep_origin      complement(3180..4140)
                     /label="pSC101** ori"
                     /ApEinfo_revcolor="#c7b0e3"
                     /ApEinfo_fwdcolor="#c7b0e3"
     terminator      4141..4246
                     /label="T0"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(4276..5105)
                     /label="Amp"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
     misc_feature    complement(5106..5131)
                     /label="Amp"
                     /ApEinfo_revcolor="#ffef86"
                     /ApEinfo_fwdcolor="#ffef86"
ORIGIN
        1 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
       61 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
      121 agtgccacct gTGGCAATTC CGACGTCTTG ACAGCTAGCT CAGTCCTAGG TATAATACTA
      181 GTAGGACGCC TTTGGTAACC GCGTTTTAGA GCTAGAAATA GCAAGTTAAA ATAAGGCTAG
      241 TCCGTTATCA ACTTGAAAAA GTGGCACATG AGGATCACCC ATGTGCTTTT TTTGAAGCTT
      301 GGGCCCGAAC AAAAACTCAT CTCAGAAGAG GATCTGAATA GCGCCGTCGA CCATCATCAT
      361 CATCATCATT GAGTTTAAAC GGTCTCCAGC TTGGCTGTTT TGGCGGATGA GAGAAGATTT
      421 TCAGCCTGAT ACAGATTAAA TCAGAACGCA GAAGCGGTCT GATAAAACAG AATTTGCCTG
      481 GCGGCAGTAG CGCGGTGGTC CCACCTGACC CCATGCCGAA CTCAGAAGTG AAACGCCGTA
      541 GCGCCGATGG TAGTGTGGGG TCTCCCCATG CGAGAGTAGG GAACTGCCAG GCATCAAATA
      601 AAACGAAAGG CTCAGTCGAA AGACTGGGCC TTTCGTTTTA TCTGTTGTTT GTCGGTGAAC
      661 TGGATCCTTA CTCGAGTCTA GACTGCAGgc ttcctcgctc actgactcgc tgcgctcggt
      721 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
      781 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagG CCAGGAACCG
      841 TAAAAACGCC TACGGTATCC ACCGGAGACC TATGGCAGCC TCCGGCCGCC ATAGGACACC
      901 TTTGGTTGCC AAGGGTGACC TATGGTGACC ATGGGCCACC ACGGGCGACC TCAGGTATCC
      961 TGCGGTGTCC TGCGGTTACC AAAGGCGTCC TTTGGGTTCC ACCGGATACC TCCGGACTTG
     1021 ACAgctagct cagtcctagg GATTGTGcta gcGAATTCAT TAAAGAGGAG AAAGGTACCA
     1081 TGGCGAGTAG CGAAGACGTT ATCAAAGAGT TCATgcgttt caaagttcgt atggaaggtt
     1141 ccgttaacgg tcacgagttc gaaatcgaag gtgaaggtga aggtcgtccg tacgaaggta
     1201 cccagaccgc taaactgaaa gttaccaaag gtggtccgct gccgttcgct tgggacatcc
     1261 tgtccccgca gttccagtac ggttccaaag cttacgttaa acacccggct gacatcccgg
     1321 actacctgaa actgtccttc ccggaaggtt tcaaatggga acgtgttatg aacttcgaag
     1381 acggtggtgt tgttaccgtt acccaggact cctccctgca agacggtgag ttcatctaca
     1441 aagttaaact gcgtggtacc aacttcccgt ccgacggtcc ggttatgcag aaaaaaacca
     1501 tgggttggga agcttccacc gaacgtatgt acccggaaga cggtgctctg aaaggtgaaa
     1561 tcaaaatgcg tctgaaactg aaagacggtg gtcactacga cgctgaagtt aaaaccacct
     1621 acatggctaa aaaaccggtt cagctgccgg gtgcttacaa aaccgacatc aaactggaca
     1681 tcacctccca caacgaagac tacaccatcg ttgaacagta cgaacgtgct gaaggtcgtc
     1741 actccaccgg tgcttaagga tccaaactcg agtaaggatc tCCAGGCATC AAATAAAACG
     1801 AAAGGCTCAG TCGAAAGACT GGGCCTTTCG TTTTATCTGT TGTTTGTCGG TGAACGCTCT
     1861 CTACTAGAGT CACACTGGCT CACCTTCGGG TGGGCCTTTC TGCGTTTATA Cctagggtac
     1921 gggttttgct gcccgcaaac gggctgttct ggtgttgcta gtttgttatc agaatcgcag
     1981 atccggcttc agccggtttg ccggctgaaa gcgctatttc ttccagaatt gccatgattt
     2041 tttccccacg ggaggcgtca ctggctcccg tgttgtcggc agctttgatt cgataagcag
     2101 catcgcctgt ttcaggctgt ctatgtgtga ctgttgagct gtaacaagtt gtctcaggtg
     2161 ttcaatttca tgttctagtt gctttgtttt actggtttca cctgttctat taggtgttac
     2221 atgctgttca tctgttacat tgtcgatctg ttcatggtga acagctttga atgcaccaaa
     2281 aactcgtaaa agctctgatg tatctatctt ttttacaccg ttttcatctg tgcatatgga
     2341 cagttttccc tttgatatgt aacggtgaac agttgttcta cttttgtttg ttagtcttga
     2401 tgcttcactg atagatacaa gagccataag aacctcagat ccttccgtat ttagccagta
     2461 tgttctctag tgtggttcgt tgtttttgcg tgagccatga gaacgaacca ttgagatcat
     2521 acttactttg catgtcactc aaaaattttg cctcaaaact ggtgagctga atttttgcag
     2581 ttaaagcatc gtgtagtgtt tttcttagtc cgttatgtag gtaggaatct gatgtaatgg
     2641 ttgttggtat tttgtcacca ttcattttta tctggttgtt ctcaagttcg gttacgagat
     2701 ccatttgtct atctagttca acttggaaaa tcaacgtatc agtcgggcgg cctcgcttat
     2761 caaccaccaa tttcatattg ctgtaagtgt ttaaatcttt acttattggt ttcaaaaccc
     2821 attggttaag ccttttaaac tcatggtagt tattttcaag cattaacatg aacttaaatt
     2881 catcaaggct aatctctata tttgccttgt gagttttctt ttgtgttagt tcttttaata
     2941 accactcata aatcctcata gagtatttgt tttcaaaaga cttaacatgt tccagattat
     3001 attttatgaa tttttttaac tggaaaagat aaggcaatat ctcttcacta aaaactaatt
     3061 ctaatttttc gcttgagaac ttggcatagt ttgtccactg gaaaatctca aagcctttaa
     3121 ccaaaggatt cctgatttcc acagttctcg tcatcagctc tctggttgct ttagctaata
     3181 caccataagc attttcccta ctgatgttca tcatctgagc gtattggtta taagtgaacg
     3241 ataccgtccg ttctttcctt gtagggtttt caatcgtggg gttgagtagt gccacacagc
     3301 ataaaattag cttggtttca tgctccgtta agtcatagcg actaatcgct agttcatttg
     3361 ctttgaaaac aactaattca gacatacatc tcaattggtc taggtgattt taatcactat
     3421 accaattgag atgggctagt caatgataat tactagtcct tttcccgggt gatctgggta
     3481 tctgtaaatt ctgctagacc tttgctggaa aacttgtaaa ttctgctaga ccctctgtaa
     3541 attccgctag acctttgtgt gttttttttg tttatattca agtggttata atttatagaa
     3601 taaagaaaga ataaaaaaag ataaaaagaa tagatcccag ccctgtgtat aactcactac
     3661 tttagtcagt tccgcagtat tacaaaagga tgtcgcaaac gctgtttgct cctctacaaa
     3721 acagacctta aaaccctaaa ggcttaagta gcaccctcgc aagctcgggc aaatcgctga
     3781 atattccttt tgtctccgac catcaggcac ctgagtcgct gtctttttcg tgacattcag
     3841 ttcgctgcgc tcacggctct ggcagtgaat gggggtaaat ggcactacag gcgcctttta
     3901 tggattcatg caaggaaact acccataata caagaaaagc ccgtcacggg cttctcaggg
     3961 cgttttatgg cgggtctgct atgtggtgct atctgacttt ttgctgttca gcagttcctg
     4021 ccctctgatt ttccagtctg accacttcgg attatcccgt gacaggtcat tcagactggc
     4081 taatgcaccc agtaaggcag cggtatcatc aacaggctta cccgtcttac tgtccctagt
     4141 gcttggattc tcaccaataa aaaacgcccg gcggcaaccg agcgttctga acaaatccag
     4201 atggagttct gaggtcatta ctggatctat caacaggagt ccaagcgagc tcgtaaactt
     4261 ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc
     4321 gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac
     4381 catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat
     4441 cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg
     4501 cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata
     4561 gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta
     4621 tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt
     4681 gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag
     4741 tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa
     4801 gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc
     4861 gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt
     4921 taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc
     4981 tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta
     5041 ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa
     5101 taagggcgac acggaaatgt tgaatactca t
//