LOCUS       AiP15692                4754 bp    DNA     circular UNA 14-OCT-2024
DEFINITION  pAAV-AiE3242m-minBG-SYFP2-WPRE3-BGHpA.
ACCESSION   urn.local.aaron.oster.5f3-it6u6jh
VERSION     urn.local.aaron.oster.5f3-it6u6jh
KEYWORDS    .
SOURCE       (pAAV-eHGT_3242m-minBglobin-SYFP2-WPRE3-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    complement(1..130)
                     /note="Geneious type: LTR"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ITR"
     regulatory      168..667
                     /note="Geneious type: Enhancer"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AiE3242m"
     regulatory      685..737
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="minBG promoter"
     regulatory      772..781
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Kozak sequence"
     CDS             778..1497
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SYFP2"
     3'UTR           1532..1777
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="WPRE3"
     misc_feature    1791..1994
                     /note="Geneious type: polyA_signal"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="BGH-pA"
     misc_feature    2017..2157
                     /note="Geneious type: LTR"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ITR"
     rep_origin      2249..2555
     regulatory      2969..3073
                     /note="Geneious type: promoter"
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR promoter"
     CDS             3074..3934
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR"
     rep_origin      4082..4749
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtctctat ctttaaaccc ttctatatga acacataggc
      181 atttgatcaa caaggagtca gtactgagta ctgtgagact tgggggaaag acaaatctaa
      241 aattttattc tgggatactt tcagggaatt ttggaaacca taaagactat aatccttgcc
      301 ctctgcttta attagttgtc atctagaaat tatatctgaa gccaacagct gggaggcaca
      361 tttagcagtt tactgaggga cccgtggacc cactgaacca cgtgcttcaa agggacactt
      421 tgtaccttct cactgagata aggaatggtt agcgattatt tatgcaacag ctaccatgaa
      481 atttgagttg aatcttgacc tcctgtatat atttccataa tgaaattgac attaatctga
      541 ataatttgtg gagagaagct aagaggtttt tttatagcaa gaggtttgtt gaaatggcta
      601 agaaccacag ccttacaact tggagacccc aaacgatcag tgtgtacctt ttcgagacat
      661 ctgctataac ctatctggga gctcgggctg ggcataaaag tcagggcaga gccatctatt
      721 gcttacattt gcttctggga tccagatctt tcgaagctag cgctaccggt cgccaccatg
      781 gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc
      841 gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc
      901 aagctgaccc tgaagctgat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc
      961 gtgaccaccc tgggctacgg cgtgcagtgc ttcgcccgct accccgacca catgaagcag
     1021 cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc
     1081 aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg
     1141 aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag
     1201 ctggagtaca actacaacag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc
     1261 atcaaggcca acttcaagat ccgccacaac atcgaggacg gcggcgtgca gctcgccgac
     1321 cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac
     1381 ctgagctacc agtccaagct gagcaaagac cccaacgaga agcgcgatca catggtcctg
     1441 ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagtc
     1501 gacggcgcgc cgcggccgcg aattcgatat cataatcaac ctctggatta caaaatttgt
     1561 gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg atacgctgct
     1621 ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc ctccttgtat
     1681 aaatcctggt tagttcttgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     1741 acaggggctc ggctgttggg cactgacaat tccgtggctc gagagatctt cgactgtgcc
     1801 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
     1861 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
     1921 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
     1981 caatagcagg catgcacgtg cggaccgagc ggccgcagga acccctagtg atggagttgg
     2041 ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac
     2101 gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc
     2161 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa
     2221 gcaaccatag tacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     2281 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     2341 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     2401 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc
     2461 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     2521 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cgggctattc
     2581 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     2641 acaaaaattt aacgcgaatt ttaacaaaat attaacgttt acaattttat ggtgcactct
     2701 cagtacaatc tgctctgatg ccgcatagtt aagccagccc cgacacccgc caacacccgc
     2761 tgacgcgccc tgacgggctt gtctgctccc ggcatccgct tacagacaag ctgtgaccgt
     2821 ctccgggagc tgcatgtgtc agaggttttc accgtcatca ccgaaacgcg cgagacgaaa
     2881 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     2941 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     3001 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     3061 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3121 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3181 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3241 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3301 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3361 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3421 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3481 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3541 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3601 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3661 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3721 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3781 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3841 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3901 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     3961 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     4021 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     4081 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4141 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4201 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     4261 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4321 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4381 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4441 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     4501 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4561 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4621 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4681 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4741 ttttgctcac atgt
//
