LOCUS       AiP1722                 4746 bp    DNA     circular UNA 01-MAY-2024
DEFINITION  pAAV-AiE2328m-minBG-SYFP2-WPRE-BGHpA.
ACCESSION   Circular
KEYWORDS    .
SOURCE       (AiP1722 - pAAV-MGT_E328-minBGpromoter-SYFP2-WPRE-BGHpA)
  ORGANISM  .
FEATURES             Location/Qualifiers
     misc_feature    complement(1..130)
                     /note="Geneious type: LTR"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ITR"
     regulatory      162..662
                     /note="Geneious type: Enhancer"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AiE2328m"
     regulatory      671..723
                     /note="Geneious type: promoter"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="minBG promoter"
     regulatory      758..767
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Kozak sequence"
     CDS             764..1483
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="SYFP2"
     3'UTR           1518..1763
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="WPRE3"
     misc_feature    1777..1980
                     /note="Geneious type: polyA_signal"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="BGH-pA"
     misc_feature    2009..2149
                     /note="Geneious type: LTR"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="ITR"
     rep_origin      2241..2547
     regulatory      2961..3065
                     /note="Geneious type: promoter"
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR promoter"
     misc_feature    2996..3003
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="FRT3 spacer (FASEBJ 2011, 25, 1)"
     CDS             3066..3923
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="AmpR"
     rep_origin      4074..4741
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttgtaag agtaataatc gatacacaga atcaatgttg
      181 aatgtattaa acaccaagga tgtttgtcta gaatttaaat aattttactt attccaggag
      241 ctaagcttcc ttttccttgg aattatttgc catatagacg agggcttcca tcagacagga
      301 cattttattt gattacactt ggatggtgta catatttaca gagtatcagt gaggcagaaa
      361 tgccctttta aaatcatgct tttggcctca atgggcccgt cagctgcaca ctgcatcaac
      421 caagtcctgt aggtccatgg ttaagcccat gtctggctct gggcttgagg ttgacatttg
      481 gactgtggtc atcagcccct ggatatgcag atatcctgga agctttgtgg actcagagat
      541 ataaaatcta aaatcttaaa aagttctatc atccgaaaag tactaatttt aaagcttagt
      601 gagagagggg gaaatcaacc acttatgatc catgaccaag ggcagcaaac agctccttgc
      661 tccggagctc gggctgggca taaaagtcag ggcagagcca tctattgctt acatttgctt
      721 ctgggatcca gatctttcga agctagcgct accggtcgcc accatggtga gcaagggcga
      781 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
      841 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
      901 gctgatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctggg
      961 ctacggcgtg cagtgcttcg cccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1021 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1081 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1141 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1201 caacagccac aacgtctata tcaccgccga caagcagaag aacggcatca aggccaactt
     1261 caagatccgc cacaacatcg aggacggcgg cgtgcagctc gccgaccact accagcagaa
     1321 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gctaccagtc
     1381 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     1441 cgccgccggg atcactctcg gcatggacga gctgtacaag taagtcgacg gcgcgccgcg
     1501 gccgcgaatt cgatatcata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     1561 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     1621 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttagt
     1681 tcttgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     1741 gttgggcact gacaattccg tggctcgaga gatcttcgac tgtgccttct agttgccagc
     1801 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     1861 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     1921 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     1981 agatctcacg tgcggaccga gcggccgcag gaacccctag tgatggagtt ggccactccc
     2041 tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc
     2101 tttgcccggg cggcctcagt gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg
     2161 cggtattttc tccttacgca tctgtgcggt atttcacacc gcatacgtca aagcaaccat
     2221 agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga
     2281 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg
     2341 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat
     2401 ttagtgcttt acggcacctc gaccccaaaa aacttgattt gggtgatggt tcacgtagtg
     2461 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata
     2521 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcgggctat tcttttgatt
     2581 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat
     2641 ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt atggtgcact ctcagtacaa
     2701 tctgctctga tgccgcatag ttaagccagc cccgacaccc gccaacaccc gctgacgcgc
     2761 cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga
     2821 gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgagacga aagggcctcg
     2881 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg
     2941 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     3001 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     3061 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     3121 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     3181 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     3241 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     3301 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     3361 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     3421 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     3481 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     3541 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     3601 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     3661 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     3721 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     3781 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     3841 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     3901 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     3961 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     4021 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     4081 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     4141 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     4201 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
     4261 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     4321 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     4381 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     4441 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     4501 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     4561 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     4621 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     4681 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     4741 acatgt
//
