LOCUS       SB-MTSH6_2GFP_C4        7373 bp    DNA     circular SYN 19-JUL-2025
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
COMMENT     ##Imported_From-START##
            Path     :: /Users/william/Dropbox/My Mac
                        (Williams-MacBook-Pro.local)/Downloads/Moore_R8L_result
                        s
            Filename :: Moore_R8L_6_SB-MTSH6_2GFP_C4_peak_2_pLann.gbk
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     CDS             join(7272..7373,1..759)
                     /note="pLannotate"
                     /standard_name="AmpR"
     rep_origin      930..1518
     misc_feature    complement(2081..2136)
                     /note="Geneious type: polyA_signal"
                     /note="pLannotate"
                     /standard_name="β-globin poly(A) signal"
     CDS             complement(2207..2287)
                     /note="pLannotate"
                     /standard_name="HBB (fragment)"
     CDS             complement(2301..2900)
                     /note="pLannotate"
                     /standard_name="PuroR"
     regulatory      3533..4714
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="EF-1α promoter"
     CDS             4746..4841
                     /note="pLannotate"
                     /standard_name="Otc (fragment)"
     CDS             4863..5699
                     /standard_name="FAP"
     CDS             5760..6482
                     /note="pLannotate"
                     /standard_name="EGFP"
     misc_feature    6511..6735
                     /note="Geneious type: polyA_signal"
                     /note="pLannotate"
                     /standard_name="bGH poly(A) signal"
     regulatory      6739..6760
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="SV40 promoter (fragment)"
     rep_origin      7059..7089
     misc_feature    complement(7090..7140)
                     /note="pLannotate"
                     /standard_name="bom (fragment)"
     regulatory      7167..7271
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="AmpR promoter"
ORIGIN      
        1 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
       61 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
      121 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
      181 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
      241 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
      301 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
      361 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
      421 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
      481 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
      541 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
      601 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
      661 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
      721 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
      781 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
      841 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
      901 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
      961 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     1021 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     1081 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     1141 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     1201 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     1261 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     1321 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     1381 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     1441 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     1501 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     1561 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     1621 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     1681 gtgagcgagg aagcggatcc ctatacagtt gaagtcggaa gtttacatac accttagcca
     1741 aatacattta aactcacttt ttcacaattc ctgacattta atcctagtaa aaattccctg
     1801 tcttaggtca gttaggatca ccactttatt ttaagaatgt gaaatatcag aataatagta
     1861 gagagaatga ttcatttcag cttttatttc tttcatcaca ttcccagtgg gtcagaagtt
     1921 tacatacact cgactcctct gcagaatgcg gcgatgtttc ggtaaggggt cccgagtagc
     1981 tagttcatgg cagccagcat atggcatatg ttgccaaact ctaaaccaaa tactcattct
     2041 gatgttttaa atgatttgcc ctcccatatg tccttccgag tgagagacac aaaaaattcc
     2101 aacacactat tgcaatgaaa ataaatttcc tttattagcc agaagtcaga tgctcaaggg
     2161 gcttcatgat gtccccataa tttttggcag agggaaaaag atctcagtgg tatttgtgag
     2221 ccagggcatt ggccacacca gccaccacct tctgataggc agcctgcacc tgaggagtga
     2281 attcacgaca ggccttcgaa tcaggcaccg ggcttgcggg tcatgcacca ggtgcgcggt
     2341 ccttcgggca cctcgacgtc ggcggtgacg gtgaagccga gccgctcgta gaaggggagg
     2401 ttgcggggcg cggaggtctc caggaaggcg ggcaccccgg cgcgctcggc cgcctccact
     2461 ccggggagca cgacggcgct gcccagaccc ttgccctggt ggtcgggcga gacgccgacg
     2521 gtggccagga accacgcggg ctccttgggc cggtgcggcg ccaggaggcc ttccatctgt
     2581 tgctgcgcgg ccagccggga accgctcaac tcggccatgc gcgggccgat ctcggcgaac
     2641 accgcccccg cttcgacgct ctccggcgtg gtccagaccg ccaccgcggc gccgtcgtcc
     2701 gcgacccaca ccttgccgat gtcgagcccg acgcgcgtga ggaagagttc ttgcagctcg
     2761 gtgacccgct cgatgtggcg gtccggatcg acggtgtggc gcgtggcggg gtagtcggcg
     2821 aacgcggcgg cgagggtgcg tacggccctg gggacgtcgt cgcgggtggc gaggcgcacc
     2881 gtgggcttgt actcggtcat ggtggcctca ggtgcagagg tttctacagg gaaagagaga
     2941 aaggctacag ttagtttgct gtgatgatgc taagtgccga ggtcagatgt ctatggattg
     3001 tacacggaat cccaacgcag gaaccaaaaa acactcaccc aaaaaaatgg cgctaaggcc
     3061 gagagaaagg cgaagttccg tctacggcta tataccttga aacaggggcg gcagaagtga
     3121 cgcaacgcgg ttgcgcggac ggcttcctgt gttttcattg gtcgaagcgt tctgcgcgtg
     3181 cgcagagatt ccccagggaa ggcgggccct cttccgaatg tccaatccgg acattcttcc
     3241 ggttggacct ccgcggtcag cgctgtgccc ggcgagagat cacgtggggc gcggaggcgg
     3301 tgctgctggg gcacggccgt ccagcctcgg cggccatatt tttgaggggc tgttcatctc
     3361 gttcacacgc tctgtccgcc atgtttgtga gtggaagcgc cattacccct tcaagcgact
     3421 gaaggctgca gggcctctgg tggcccgcat ggggagacca gacccgccag gcccgccttt
     3481 ccgcactcag tccgggctta ctttattttg tgagacaggg tctcgcctag aggctccggt
     3541 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     3601 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     3661 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     3721 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
     3781 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacg
     3841 cccctggctg cagtacgtga ttcttgatcc cgagcttcgg gttggaagtg ggtgggagag
     3901 ttcgaggcct tgcgcttaag gagccccttc gcctcgtgct tgagttgagg cctggcttgg
     3961 gcgctggggc cgccgcgtgc gaatctggtg gcaccttcgc gcctgtctcg ctgctttcga
     4021 taagtctcta gccatttaaa atttttgatg acctgctgcg acgctttttt tctggcaaga
     4081 tagtcttgta aatgcgggcc aagatctgca cactggtatt tcggtttttg gggccgcggg
     4141 cggcgacggg gcccgtgcgt cccagcgcac atgttcggcg aggcggggcc tgcgagcgcg
     4201 gccaccgaga atcggacggg ggtagtctca agctggccgg cctgctctgg tgcctggcct
     4261 cgcgccgccg tgtatcgccc cgccctgggc ggcaaggctg gcccggtcgg caccagttgc
     4321 gtgagcggaa agatggccgc ttcccggccc tgctgcaggg agctcaaaat ggaggacgcg
     4381 gcgctcggga gagcgggcgg gtgagtcacc cacacaaagg aaaagggcct ttccgtcctc
     4441 agccgtcgct tcatgtgact ccacggagta ccgggcgccg tccaggcacc tcgattagtt
     4501 ctcgagcttt tggagtacgt cgtctttagg ttggggggag gggttttatg cgatggagtt
     4561 tccccatact gagtgggtgg agactgaagt taggccagct tggcacttga tgtaattctc
     4621 cttggaattt gccctttttg agtttggatc ttggttcatt ctcaagcctc agacagtggt
     4681 tcaaagtttt tttcttccat ttaaggtgtc gtgaaaacta ccccaagctg gcctctgagg
     4741 ccaccatgct gtctaatttg agaatcctgc tcaacaaggc agctcttaga aaggctcaca
     4801 cttccatggt tcgaaatttt cggtatggga agccagtcca ggaattcggc ctttctggcc
     4861 tgcaagtcca gttgcaagaa tctggacctg gtttggttaa accatctgag acactttccc
     4921 taacctgcac agtcagtggc gcatcaattt cctcctcgca ctattattgg ggttggatta
     4981 gacaaccacc tggaaagggc ccagagtgga tagggtctat gtactactcg ggtagaactt
     5041 actataatcc tgccttgaag tcacgtgtta ccatatcctc tgataagtca aaaaaccaat
     5101 tctttttaaa actcactagc gttaccgctg ctgatacagc tgtctattat gctgcccgtg
     5161 agggcccaac gcattattat gataattcag gcccaatacc tagcgatgag tattttcaac
     5221 attggggcca gggcacttta gttacagttt ccggtggtgg cggctctggt ggcggtggca
     5281 gcggcggtgg tggttccgga ggcggcggtt ctcaggtgca gctgcaggag tcgggcccag
     5341 gactggtgaa gccttcggag accctgtccc tcacctgcac tgtctctggt gcctccatca
     5401 gcagtagtca ttactactgg ggctggattc gccagccccc agggaagggg cctgagtgga
     5461 ttgggagtat gtattatagt gggagaacgt actacaaccc ggccctcaag agtcgagtca
     5521 ccatatcatc agacaagtcg aagaaccagt tcttcttgaa gttgacctct gtaaccgccg
     5581 cggacacggc cgtgtattac gctgcgaggg agggacccac acattactat gataatagtg
     5641 gtccaatacc ttcggatgag tatttccagc actggggcca gggtaccctg gtcactgtct
     5701 ccgcggccgg tctggcccat atgggaggcg gtgggtctgg tggcggtggc agcaccggta
     5761 tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc catcctggtc gagctggacg
     5821 gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg
     5881 gcaagctgac cctgaagttc atctgcacca ccggcaagct gcccgtgccc tggcccaccc
     5941 tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc
     6001 agcacgactt cttcaagtcc gccatgcccg aaggctacgt ccaggagcgc accatcttct
     6061 tcaaggacga cggcaactac aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg
     6121 tgaaccgcat cgagctgaag ggcatcgact tcaaggagga cggcaacatc ctggggcaca
     6181 agctggagta caactacaac agccacaacg tctatatcat ggccgacaag cagaagaacg
     6241 gcatcaaggt gaacttcaag atccgccaca acatcgagga cggcagcgtg cagctcgccg
     6301 accactacca gcagaacacc cccatcggcg acggccccgt gctgctgccc gacaaccact
     6361 acctgagcac ccagtccgcc ctgagcaaag accccaacga gaagcgcgat cacatggtcc
     6421 tgctggagtt cgtgaccgcc gccgggatca ctctcggcat ggacgagctg tacaagctcg
     6481 agtaatctag agctcgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg
     6541 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     6601 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     6661 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg
     6721 cggtgggctc tatggcttct gaggcggaaa gaaccagctg ggcctactag atagcggacc
     6781 ccttaccgaa acatcgccgc attctgcaga ggagtcgaac ttagtgtatg taaacttctg
     6841 acccactgga attgtgatac agtgaattat aagtgaaata atctgtctgt aaacaattgt
     6901 tggaaaaatg acttgtgtca tgcacaaagt agatgtccta actgacttgc caaaactatt
     6961 gtttgttaac aagaaatttg tggagtagtt gaaaaacgag ttttaatgac tccaacttaa
     7021 gtgtatgtaa acttccgact tcaactgtat agggatccaa ttttaacaaa atattaacgc
     7081 ttacaatttc ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcat
     7141 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     7201 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     7261 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     7321 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gct
//
