LOCUS       SB-H6_2GFPKDEL_C1_        7352 bp    DNA     circular SYN 19-JUL-2025
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
COMMENT     ##Imported_From-START##
            Path     :: /Users/william/Dropbox/My Mac
                        (Williams-MacBook-Pro.local)/Downloads/Moore_R8L_result
                        s
            Filename :: Moore_R8L_1_SB-H6_2GFPKDEL_C1_pLann.gbk
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     CDS             join(6683..7352,1..191)
                     /note="pLannotate"
                     /standard_name="AmpR"
     rep_origin      362..950
     misc_feature    complement(1513..1568)
                     /note="Geneious type: polyA_signal"
                     /note="pLannotate"
                     /standard_name="β-globin poly(A) signal"
     CDS             complement(1639..1719)
                     /note="pLannotate"
                     /standard_name="HBB (fragment)"
     CDS             complement(1733..2332)
                     /note="pLannotate"
                     /standard_name="PuroR"
     regulatory      2965..4146
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="EF-1α promoter"
     sig_peptide     4178..4240
                     /note="pLannotate"
                     /standard_name="Igκ leader"
     CDS             4262..5098
                     /standard_name="FAP"
     CDS             5159..5881
                     /note="pLannotate"
                     /standard_name="EGFP"
     CDS             5882..5893
                     /standard_name="KDEL"
     misc_feature    5922..6146
                     /note="Geneious type: polyA_signal"
                     /note="pLannotate"
                     /standard_name="bGH poly(A) signal"
     regulatory      6150..6171
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="SV40 promoter (fragment)"
     rep_origin      6470..6500
     misc_feature    complement(6501..6551)
                     /note="pLannotate"
                     /standard_name="bom (fragment)"
     regulatory      6578..6682
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="AmpR promoter"
ORIGIN      
        1 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
       61 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
      121 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
      181 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
      241 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
      301 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
      361 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
      421 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
      481 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
      541 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
      601 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
      661 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
      721 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
      781 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
      841 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
      901 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
      961 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1021 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     1081 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggat ccctatacag
     1141 ttgaagtcgg aagtttacat acaccttagc caaatacatt taaactcact ttttcacaat
     1201 tcctgacatt taatcctagt aaaaattccc tgtcttaggt cagttaggat caccacttta
     1261 ttttaagaat gtgaaatatc agaataatag tagagagaat gattcatttc agcttttatt
     1321 tctttcatca cattcccagt gggtcagaag tttacataca ctcgactcct ctgcagaatg
     1381 cggcgatgtt tcggtaaggg gtcccgagta gctagttcat ggcagccagc atatggcata
     1441 tgttgccaaa ctctaaacca aatactcatt ctgatgtttt aaatgatttg ccctcccata
     1501 tgtccttccg agtgagagac acaaaaaatt ccaacacact attgcaatga aaataaattt
     1561 cctttattag ccagaagtca gatgctcaag gggcttcatg atgtccccat aatttttggc
     1621 agagggaaaa agatctcagt ggtatttgtg agccagggca ttggccacac cagccaccac
     1681 cttctgatag gcagcctgca cctgaggagt gaattcacga caggccttcg aatcaggcac
     1741 cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg cacctcgacg tcggcggtga
     1801 cggtgaagcc gagccgctcg tagaagggga ggttgcgggg cgcggaggtc tccaggaagg
     1861 cgggcacccc ggcgcgctcg gccgcctcca ctccggggag cacgacggcg ctgcccagac
     1921 ccttgccctg gtggtcgggc gagacgccga cggtggccag gaaccacgcg ggctccttgg
     1981 gccggtgcgg cgccaggagg ccttccatct gttgctgcgc ggccagccgg gaaccgctca
     2041 actcggccat gcgcgggccg atctcggcga acaccgcccc cgcttcgacg ctctccggcg
     2101 tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca caccttgccg atgtcgagcc
     2161 cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg ctcgatgtgg cggtccggat
     2221 cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc ggcgagggtg cgtacggccc
     2281 tggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt gtactcggtc atggtggcct
     2341 caggtgcaga ggtttctaca gggaaagaga gaaaggctac agttagtttg ctgtgatgat
     2401 gctaagtgcc gaggtcagat gtctatggat tgtacacgga atcccaacgc aggaaccaaa
     2461 aaacactcac ccaaaaaaat ggcgctaagg ccgagagaaa ggcgaagttc cgtctacggc
     2521 tatatacctt gaaacagggg cggcagaagt gacgcaacgc ggttgcgcgg acggcttcct
     2581 gtgttttcat tggtcgaagc gttctgcgcg tgcgcagaga ttccccaggg aaggcgggcc
     2641 ctcttccgaa tgtccaatcc ggacattctt ccggttggac ctccgcggtc agcgctgtgc
     2701 ccggcgagag atcacgtggg gcgcggaggc ggtgctgctg gggcacggcc gtccagcctc
     2761 ggcggccata tttttgaggg gctgttcatc tcgttcacac gctctgtccg ccatgtttgt
     2821 gagtggaagc gccattaccc cttcaagcga ctgaaggctg cagggcctct ggtggcccgc
     2881 atggggagac cagacccgcc aggcccgcct ttccgcactc agtccgggct tactttattt
     2941 tgtgagacag ggtctcgcct agaggctccg gtgcccgtca gtgggcagag cgcacatcgc
     3001 ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc tagagaaggt
     3061 ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt cccgagggtg
     3121 ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg
     3181 ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt
     3241 atggcccttg cgtgccttga attacttcca cgcccctggc tgcagtacgt gattcttgat
     3301 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
     3361 tcgcctcgtg cttgagttga ggcctggctt gggcgctggg gccgccgcgt gcgaatctgg
     3421 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
     3481 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
     3541 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
     3601 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
     3661 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
     3721 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
     3781 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
     3841 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
     3901 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
     3961 ggttgggggg aggggtttta tgcgatggag tttccccata ctgagtgggt ggagactgaa
     4021 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
     4081 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttaaggtg
     4141 tcgtgaaaac taccccaagc tggcctctga ggccaccatg gagacagaca cactcctgct
     4201 atgggtactg ctgctctggg ttccaggttc cactggtgac gaattcggcc tttctggcct
     4261 gcaagtccag ttgcaagaat ctggacctgg tttggttaaa ccatctgaga cactttccct
     4321 aacctgcaca gtcagtggcg catcaatttc ctcctcgcac tattattggg gttggattag
     4381 acaaccacct ggaaagggcc cagagtggat agggtctatg tactactcgg gtagaactta
     4441 ctataatcct gccttgaagt cacgtgttac catatcctct gataagtcaa aaaaccaatt
     4501 ctttttaaaa ctcactagcg ttaccgctgc tgatacagct gtctattatg ctgcccgtga
     4561 gggcccaacg cattattatg ataattcagg cccaatacct agcgatgagt attttcaaca
     4621 ttggggccag ggcactttag ttacagtttc cggtggtggc ggctctggtg gcggtggcag
     4681 cggcggtggt ggttccggag gcggcggttc tcaggtgcag ctgcaggagt cgggcccagg
     4741 actggtgaag ccttcggaga ccctgtccct cacctgcact gtctctggtg cctccatcag
     4801 cagtagtcat tactactggg gctggattcg ccagccccca gggaaggggc ctgagtggat
     4861 tgggagtatg tattatagtg ggagaacgta ctacaacccg gccctcaaga gtcgagtcac
     4921 catatcatca gacaagtcga agaaccagtt cttcttgaag ttgacctctg taaccgccgc
     4981 ggacacggcc gtgtattacg ctgcgaggga gggacccaca cattactatg ataatagtgg
     5041 tccaatacct tcggatgagt atttccagca ctggggccag ggtaccctgg tcactgtctc
     5101 cgcggccggt ctggcccata tgggaggcgg tgggtctggt ggcggtggca gcaccggtat
     5161 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
     5221 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg
     5281 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
     5341 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
     5401 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt
     5461 caaggacgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
     5521 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
     5581 gctggagtac aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg
     5641 catcaaggtg aacttcaaga tccgccacaa catcgaggac ggcagcgtgc agctcgccga
     5701 ccactaccag cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta
     5761 cctgagcacc cagtccgccc tgagcaaaga ccccaacgag aagcgcgatc acatggtcct
     5821 gctggagttc gtgaccgccg ccgggatcac tctcggcatg gacgagctgt acaagctcga
     5881 gaaggatgaa ctgtaatcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     5941 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6001 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     6061 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     6121 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct gggcctacta
     6181 gatagcggac cccttaccga aacatcgccg cattctgcag aggagtcgaa cttagtgtat
     6241 gtaaacttct gacccactgg aattgtgata cagtgaatta taagtgaaat aatctgtctg
     6301 taaacaattg ttggaaaaat gacttgtgtc atgcacaaag tagatgtcct aactgacttg
     6361 ccaaaactat tgtttgttaa caagaaattt gtggagtagt tgaaaaacga gttttaatga
     6421 ctccaactta agtgtatgta aacttccgac ttcaactgta tagggatcca attttaacaa
     6481 aatattaacg cttacaattt cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     6541 tcacaccgca tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     6601 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     6661 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     6721 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     6781 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     6841 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     6901 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     6961 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     7021 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     7081 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     7141 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     7201 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     7261 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     7321 agttgcagga ccacttctgc gctcggccct tc
//
