LOCUS       pTet_FAPtgnGFP-1_(reversed)        7429 bp    DNA     circular SYN 19-JUL-2025
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
COMMENT     ##Imported_From-START##
            Path     :: /Users/william/Dropbox/My Mac
                        (Williams-MacBook-Pro.local)/Downloads/Moore_P2H_result
                        s
            Filename :: Moore_P2H_1_pTet_FAPtgnGFP-1_pLann.gbk
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     rep_origin      join(complement(1..42),complement(6883..7429))
     CDS             complement(213..1073)
                     /note="pLannotate"
                     /standard_name="AmpR"
     primer_bind     836..855
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2025.1.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Amp-R"
     regulatory      complement(1074..1178)
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="AmpR promoter"
     misc_feature    1205..1255
                     /note="pLannotate"
                     /standard_name="bom (fragment)"
     rep_origin      complement(1256..1286)
     regulatory      1581..1893
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="tight TRE promoter"
     CDS             1972..2019
                     /note="pLannotate"
                     /standard_name="VSV-G tsO45 (fragment)"
     CDS             2056..2892
                     /note="Derived using Geneious Prime 2025.1.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="FAP"
     CDS             2929..3090
                     /standard_name="TGN38"
     CDS             3100..3816
                     /note="pLannotate"
                     /standard_name="EGFP"
     CDS             3844..3894
                     /note="pLannotate"
                     /standard_name="VP1_SV40 (fragment)"
     misc_feature    complement(3891..3972)
                     /note="Geneious type: polyA_signal"
                     /note="pLannotate"
                     /standard_name="SV40 poly(A) signal"
     CDS             complement(3995..4063)
                     /note="pLannotate"
                     /standard_name="large T antigen (fragment)"
     CDS             4695..5392
                     /note="pLannotate"
                     /standard_name="rtTA3 (fragment)"
     CDS             5322..5432
                     /note="pLannotate"
                     /standard_name="VP48"
     primer_bind     complement(5502..5521)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2025.1.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Puro-R"
     CDS             5502..6101
                     /note="pLannotate"
                     /standard_name="PuroR"
     primer_bind     5998..6018
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2025.1.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="Puro-F"
     primer_bind     6141..6159
                     /note="SB-IL2-Vect_fwd"
                     /note="gene-specific Tm: 68.2  Ta: 65.1"
                     /note="gene-specific primer: GAGCTCGCTGATCAGCCTC"
                     /note="Derived using Geneious Prime 2025.1.3 'Annotate
                     from Database' based on nucleotide similarity"
     primer_bind     complement(6156..6173)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2025.1.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="BGH-rev"
     misc_feature    6162..6386
                     /note="Geneious type: polyA_signal"
                     /note="pLannotate"
                     /standard_name="bGH poly(A) signal"
     misc_feature    6162..6386
                     /note="Geneious type: polyA_signal"
                     /note="bovine growth hormone polyadenylation signal"
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2025.1.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="bGH poly(A) signal"
     regulatory      6390..6411
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="SV40 promoter (fragment)"
     primer_bind     complement(6712..6729)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2025.1.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="L4440"
     primer_bind     complement(6963..6982)
                     /note="Derived using Geneious Prime 2022.0.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /note="Derived using Geneious Prime 2025.1.3 'Annotate
                     from Database' based on nucleotide similarity"
                     /standard_name="pBR322ori-F"
ORIGIN      
        1 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
       61 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
      241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
      301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
      361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
      421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
      481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
      541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
      601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
      661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
      721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
      781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
      841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
      961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1201 cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     1261 gtaagcgtta atattttgtt aaaattggat ccctatacag ttgaagtcgg aagtttacat
     1321 acacttaagt tggagtcatt aaaactcgtt tttcaactac tccacaaatt tcttgttaac
     1381 aaacaatagt tttggcaagt cagttaggac atctactttg tgcatgacac aagtcatttt
     1441 tccaacaatt gtttacagac agattatttc acttataatt cactgtatca caattccagt
     1501 gggtcagaag tttacataca ctaagttcga ctcctctgca gaatgcggcg atgtttcggt
     1561 aaggggtccg ctatctagac gagtttactc cctatcagtg atagagaacg tatgtcgagt
     1621 ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac
     1681 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
     1741 gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt
     1801 actccctatc agtgatagag aacgtatgtc gattaggcgt gtacggtggg cgcctataaa
     1861 agcagagctc gtttagtgaa ccgtcagatc gcctggagcc aattccaact cttttgcctt
     1921 atacctactt tccgtaccac ttcctaccct cgaaaggcct ctgaggccac catgaagtgc
     1981 cttttgtact tagctttttt attcatcggg gtgaattgcg caccggtcgc cacctctttc
     2041 ggcctttctg gcctgcaagt ccagttgcaa gaatctggac ctggtttggt taaaccatct
     2101 gagacacttt ccctaacctg cacagtcagt ggcgcatcaa tttcctcctc gcactattat
     2161 tggggttgga ttagacaacc acctggaaag ggcccagagt ggatagggtc tatgtactac
     2221 tcgggtagaa cttactataa tcctgccttg aagtcacgtg ttaccatatc ctctgataag
     2281 tcaaaaaacc aattcttttt aaaactcact agcgttaccg ctgctgatac agctgtctat
     2341 tatgctgccc gtgagggccc aacgcattat tatgataatt caggcccaat acctagcgat
     2401 gagtattttc aacattgggg ccagggcact ttagttacag tttccggtgg tggcggctct
     2461 ggtggcggtg gcagcggcgg tggtggttcc ggaggcggcg gttctcaggt gcagctgcag
     2521 gagtcgggcc caggactggt gaagccttcg gagaccctgt ccctcacctg cactgtctct
     2581 ggtgcctcca tcagcagtag tcattactac tggggctgga ttcgccagcc cccagggaag
     2641 gggcctgagt ggattgggag tatgtattat agtgggagaa cgtactacaa cccggccctc
     2701 aagagtcgag tcaccatatc atcagacaag tcgaagaacc agttcttctt gaagttgacc
     2761 tctgtaaccg ccgcggacac ggccgtgtat tacgctgcga gggagggacc cacacattac
     2821 tatgataata gtggtccaat accttcggat gagtatttcc agcactgggg ccagggtacc
     2881 ctggtcactg tctccgcggc cggtctggcc catgggtctg gatctggtca cttctttgct
     2941 tatctggtga ccgctgctgt tcttgttgct gtcctctata ttgcttacca caacaaacga
     3001 aagattattg cttttgccct ggaaggaaaa agatccaaag tcactcggag gccaaaggcc
     3061 agtgactacc aacgtttgaa cctaaagctt ggtggtggca tggtgagcaa gggcgaggag
     3121 ctgttcaccg gggtggtgcc catcctggtc gagctggacg gcgacgtaaa cggccacaag
     3181 ttcagcgtgt ccggcgaggg cgagggcgat gccacctacg gcaagctgac cctgaagttc
     3241 atctgcacca ccggcaagct gcccgtgccc tggcccaccc tcgtgaccac cctgacctac
     3301 ggcgtgcagt gcttcagccg ctaccccgac cacatgaagc agcacgactt cttcaagtcc
     3361 gccatgcccg aaggctacgt ccaggagcgc accatcttct tcaaggacga cggcaactac
     3421 aagacccgcg ccgaggtgaa gttcgagggc gacaccctgg tgaaccgcat cgagctgaag
     3481 ggcatcgact tcaaggagga cggcaacatc ctggggcaca agctggagta caactacaac
     3541 agccacaacg tctatatcat ggccgacaag cagaagaacg gcatcaaggt gaacttcaag
     3601 atccgccaca acatcgagga cggcagcgtg cagctcgccg accactacca gcagaacacc
     3661 cccatcggcg acggccccgt gctgctgccc gacaaccact acctgagcac ccagtccgcc
     3721 ctgagcaaag accccaacga gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc
     3781 gccgggatca ctctcggcat ggacgagctg tacaagtagt gtcaggccaa gcttccatcg
     3841 atagacatga taagatacat tgatgagttt ggacaaacca caacaagaat gcagtgaaaa
     3901 aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat tataagctgc
     3961 aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca gggggagatg
     4021 tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg gtacctaggc gagaccctgt
     4081 ctcacaaaat aaagtaagcc cggactgagt gcggaaaggc gggcctggcg ggtctggtct
     4141 ccccatgcgg gccaccagag gccctgcagc cttcagtcgc ttgaaggggt aatggcgctt
     4201 ccactcacaa acatggcgga cagagcgtgt gaacgagatg aacagcccct caaaaatatg
     4261 gccgccgagg ctggacggcc gtgccccagc agcaccgcct ccgcgcccca cgtgatctct
     4321 cgccgggcac agcgctgacc gcggaggtcc aaccggaaga atgtccggat tggacattcg
     4381 gaagagggcc cgccttccct ggggaatctc tgcgcacgcg cagaacgctt cgaccaatga
     4441 aaacacagga agccgtccgc gcaaccgcgt tgcgtcactt ctgccgcccc tgtttcaagg
     4501 tatatagccg tagacggaac ttcgcctttc tctcggcctt agcgccattt ttttgggtga
     4561 gtgttttttg gttcctgcgt tgggattccg tgtacaatcc atagacatct gacctcggca
     4621 cttagcatca tcacagcaaa ctaactgtag cctttctctc tttccctgta gaaacctctg
     4681 cacctgaggc caccatgtct agactggaca agagcaaagt cataaacggc gctctggaat
     4741 tactcaatgg agtcggtatc gaaggcctga cgacaaggaa actcgctcaa aagctgggag
     4801 ttgagcagcc taccctgtac tggcacgtga agaacaagcg ggccctgctc gatgccctgc
     4861 caatcgagat gctggacagg catcataccc acttctgccc cctggaaggc gagtcatggc
     4921 aagactttct gcggaacaac gccaagtcat tccgctgtgc tctcctctca catcgcgacg
     4981 gggctaaagt gcatctcggc acccgcccaa cagagaaaca gtacgaaacc ctggaaaatc
     5041 agctcgcgtt cctgtgtcag caaggcttct ccctggagaa cgcactgtac gctctgtccg
     5101 ccgtgggcca ctttacactg ggctgcgtat tggaggaaca ggagcatcaa gtagcaaaag
     5161 aggaaagaga gacacctacc accgattcta tgcccccact tctgagacaa gcaattgagc
     5221 tgttcgaccg gcagggagcc gaacctgcct tccttttcgg cctggaacta atcatatgtg
     5281 gcctggagaa acagctaaag tgcgaaagcg gcgggccggc cgacgccctt gacgattttg
     5341 acttagacat gctcccagcc gatgcccttg acgactttga ccttgatatg ctgcctgctg
     5401 acgctcttga cgattttgac cttgacatgc tccccgggtc cggcgctact aacttcagcc
     5461 tgctgaagca ggctggtgac gtcgaggaga atcctggtcc catgaccgag tacaagccca
     5521 cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt
     5581 tcgccgacta ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca
     5641 ccgagctgca agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg
     5701 cggacgacgg cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg
     5761 tgttcgccga gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc
     5821 aacagatgga aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca
     5881 ccgtcggcgt ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg
     5941 gagtggaggc ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca
     6001 acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag
     6061 gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg attcgaaggc ctgtcgtgaa
     6121 gcttggggat caattctcta gagctcgctg atcagcctcg actgtgcctt ctagttgcca
     6181 gccatctgtt gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac
     6241 tgtcctttcc taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat
     6301 tctggggggt ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca
     6361 tgctggggat gcggtgggct ctatggcttc tgaggcggaa agaaccagct gggcctacta
     6421 gctactcggg accccttacc gaaacatcgc cgcattctgc agaggagtcg agtgtatgta
     6481 aacttctgac ccactgggaa tgtgatgaaa gaaataaaag ctgaaatgaa tcattctctc
     6541 tactattatt ctgatatttc acattcttaa aataaagtgg tgatcctaac tgacctaaga
     6601 cagggaattt ttactaggat taaatgtcag gaattgtgaa aaagtgagtt taaatgtatt
     6661 tggctaaggt gtatgtaaac ttccgacttc aactgtatag ggatccgctt cctcgctcac
     6721 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt
     6781 aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca
     6841 gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc
     6901 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     6961 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7021 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag
     7081 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7141 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7201 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7261 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7321 aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7381 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggttttttt
//
