LOCUS       pTet_GFPtgnFAP-1_(reversed)        7483 bp    DNA     circular SYN 19-JUL-2025
DEFINITION  .
ACCESSION
VERSION     .
KEYWORDS    .
SOURCE      .
  ORGANISM  .
COMMENT     ##Imported_From-START##
            Path     :: /Users/william/Dropbox/My Mac
                        (Williams-MacBook-Pro.local)/Downloads/Moore_P2H_result
                        s
            Filename :: Moore_P2H_7_pTet_GFPtgnFAP-1_pLann.gbk
            ##Imported_From-END##
FEATURES             Location/Qualifiers
     rep_origin      join(complement(1..42),complement(6937..7483))
     CDS             complement(213..1073)
                     /note="pLannotate"
                     /standard_name="AmpR"
     regulatory      complement(1074..1178)
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="AmpR promoter"
     misc_feature    1205..1255
                     /note="pLannotate"
                     /standard_name="bom (fragment)"
     rep_origin      complement(1256..1286)
     regulatory      1581..1893
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="tight TRE promoter"
     CDS             1972..2019
                     /note="pLannotate"
                     /standard_name="VSV-G tsO45 (fragment)"
     CDS             2035..2751
                     /note="pLannotate"
                     /standard_name="EGFP"
     misc_feature    2752..2796
                     /note="pLannotate"
                     /standard_name="MCS (fragment)"
     CDS             2797..2958
                     /standard_name="TGN38"
     CDS             2995..3831
                     /standard_name="FAP"
     CDS             3898..3948
                     /note="pLannotate"
                     /standard_name="VP1_SV40 (fragment)"
     misc_feature    complement(3945..4026)
                     /note="Geneious type: polyA_signal"
                     /note="pLannotate"
                     /standard_name="SV40 poly(A) signal"
     CDS             complement(4049..4117)
                     /note="pLannotate"
                     /standard_name="large T antigen (fragment)"
     CDS             4749..5446
                     /note="pLannotate"
                     /standard_name="rtTA3 (fragment)"
     CDS             5376..5486
                     /note="pLannotate"
                     /standard_name="VP48"
     CDS             5556..6155
                     /note="pLannotate"
                     /standard_name="PuroR"
     misc_feature    6216..6440
                     /note="Geneious type: polyA_signal"
                     /note="pLannotate"
                     /standard_name="bGH poly(A) signal"
     regulatory      6444..6465
                     /note="Geneious type: promoter"
                     /note="pLannotate"
                     /standard_name="SV40 promoter (fragment)"
ORIGIN      
        1 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
       61 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      121 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      181 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
      241 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
      301 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
      361 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
      421 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
      481 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
      541 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
      601 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
      661 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
      721 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
      781 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
      841 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
      901 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
      961 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1021 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1081 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1141 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1201 cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca tcaggaaatt
     1261 gtaagcgtta atattttgtt aaaattggat ccctatacag ttgaagtcgg aagtttacat
     1321 acacttaagt tggagtcatt aaaactcgtt tttcaactac tccacaaatt tcttgttaac
     1381 aaacaatagt tttggcaagt cagttaggac atctactttg tgcatgacac aagtcatttt
     1441 tccaacaatt gtttacagac agattatttc acttataatt cactgtatca caattccagt
     1501 gggtcagaag tttacataca ctaagttcga ctcctctgca gaatgcggcg atgtttcggt
     1561 aaggggtccg ctatctagac gagtttactc cctatcagtg atagagaacg tatgtcgagt
     1621 ttactcccta tcagtgatag agaacgatgt cgagtttact ccctatcagt gatagagaac
     1681 gtatgtcgag tttactccct atcagtgata gagaacgtat gtcgagttta ctccctatca
     1741 gtgatagaga acgtatgtcg agtttatccc tatcagtgat agagaacgta tgtcgagttt
     1801 actccctatc agtgatagag aacgtatgtc gattaggcgt gtacggtggg cgcctataaa
     1861 agcagagctc gtttagtgaa ccgtcagatc gcctggagcc aattccaact cttttgcctt
     1921 atacctactt tccgtaccac ttcctaccct cgaaaggcct ctgaggccac catgaagtgc
     1981 cttttgtact tagctttttt attcatcggg gtgaattgcg caccggtcgc caccatggtg
     2041 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     2101 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     2161 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     2221 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     2281 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     2341 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     2401 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     2461 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     2521 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     2581 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     2641 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     2701 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc
     2761 agatctcgag ctcaagcttc gaattctgca gtcgaccact tctttgctta tctggtgacc
     2821 gctgctgttc ttgttgctgt cctctatatt gcttaccaca acaaacgaaa gattattgct
     2881 tttgccctgg aaggaaaaag atccaaagtc actcggaggc caaaggccag tgactaccaa
     2941 cgtttgaacc taaagctttc tggaggcggt gggtctttcg gcctttctgg cctgcaagtc
     3001 cagttgcaag aatctggacc tggtttggtt aaaccatctg agacactttc cctaacctgc
     3061 acagtcagtg gcgcatcaat ttcctcctcg cactattatt ggggttggat tagacaacca
     3121 cctggaaagg gcccagagtg gatagggtct atgtactact cgggtagaac ttactataat
     3181 cctgccttga agtcacgtgt taccatatcc tctgataagt caaaaaacca attcttttta
     3241 aaactcacta gcgttaccgc tgctgataca gctgtctatt atgctgcccg tgagggccca
     3301 acgcattatt atgataattc aggcccaata cctagcgatg agtattttca acattggggc
     3361 cagggcactt tagttacagt ttccggtggt ggcggctctg gtggcggtgg cagcggcggt
     3421 ggtggttccg gaggcggcgg ttctcaggtg cagctgcagg agtcgggccc aggactggtg
     3481 aagccttcgg agaccctgtc cctcacctgc actgtctctg gtgcctccat cagcagtagt
     3541 cattactact ggggctggat tcgccagccc ccagggaagg ggcctgagtg gattgggagt
     3601 atgtattata gtgggagaac gtactacaac ccggccctca agagtcgagt caccatatca
     3661 tcagacaagt cgaagaacca gttcttcttg aagttgacct ctgtaaccgc cgcggacacg
     3721 gccgtgtatt acgctgcgag ggagggaccc acacattact atgataatag tggtccaata
     3781 ccttcggatg agtatttcca gcactggggc cagggtaccc tggtcactgt ctccgcggcc
     3841 ggtctggccc atggatccac cggatctaga taatgtcagg ccaagcttcc atcgatagac
     3901 atgataagat acattgatga gtttggacaa accacaacaa gaatgcagtg aaaaaaatgc
     3961 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     4021 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     4081 gttttttaaa gcaagtaaaa cctctacaaa tgtggtacct aggcgagacc ctgtctcaca
     4141 aaataaagta agcccggact gagtgcggaa aggcgggcct ggcgggtctg gtctccccat
     4201 gcgggccacc agaggccctg cagccttcag tcgcttgaag gggtaatggc gcttccactc
     4261 acaaacatgg cggacagagc gtgtgaacga gatgaacagc ccctcaaaaa tatggccgcc
     4321 gaggctggac ggccgtgccc cagcagcacc gcctccgcgc cccacgtgat ctctcgccgg
     4381 gcacagcgct gaccgcggag gtccaaccgg aagaatgtcc ggattggaca ttcggaagag
     4441 ggcccgcctt ccctggggaa tctctgcgca cgcgcagaac gcttcgacca atgaaaacac
     4501 aggaagccgt ccgcgcaacc gcgttgcgtc acttctgccg cccctgtttc aaggtatata
     4561 gccgtagacg gaacttcgcc tttctctcgg ccttagcgcc atttttttgg gtgagtgttt
     4621 tttggttcct gcgttgggat tccgtgtaca atccatagac atctgacctc ggcacttagc
     4681 atcatcacag caaactaact gtagcctttc tctctttccc tgtagaaacc tctgcacctg
     4741 aggccaccat gtctagactg gacaagagca aagtcataaa cggcgctctg gaattactca
     4801 atggagtcgg tatcgaaggc ctgacgacaa ggaaactcgc tcaaaagctg ggagttgagc
     4861 agcctaccct gtactggcac gtgaagaaca agcgggccct gctcgatgcc ctgccaatcg
     4921 agatgctgga caggcatcat acccacttct gccccctgga aggcgagtca tggcaagact
     4981 ttctgcggaa caacgccaag tcattccgct gtgctctcct ctcacatcgc gacggggcta
     5041 aagtgcatct cggcacccgc ccaacagaga aacagtacga aaccctggaa aatcagctcg
     5101 cgttcctgtg tcagcaaggc ttctccctgg agaacgcact gtacgctctg tccgccgtgg
     5161 gccactttac actgggctgc gtattggagg aacaggagca tcaagtagca aaagaggaaa
     5221 gagagacacc taccaccgat tctatgcccc cacttctgag acaagcaatt gagctgttcg
     5281 accggcaggg agccgaacct gccttccttt tcggcctgga actaatcata tgtggcctgg
     5341 agaaacagct aaagtgcgaa agcggcgggc cggccgacgc ccttgacgat tttgacttag
     5401 acatgctccc agccgatgcc cttgacgact ttgaccttga tatgctgcct gctgacgctc
     5461 ttgacgattt tgaccttgac atgctccccg ggtccggcgc tactaacttc agcctgctga
     5521 agcaggctgg tgacgtcgag gagaatcctg gtcccatgac cgagtacaag cccacggtgc
     5581 gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg
     5641 actaccccgc cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc
     5701 tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg
     5761 acggcgccgc ggtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg
     5821 ccgagatcgg cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga
     5881 tggaaggcct cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg
     5941 gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg
     6001 aggcggccga gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc
     6061 ccttctacga gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc
     6121 gcacctggtg catgacccgc aagcccggtg cctgattcga aggcctgtcg tgaagcttgg
     6181 ggatcaattc tctagagctc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     6241 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     6301 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     6361 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     6421 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctgggcct actagctact
     6481 cgggacccct taccgaaaca tcgccgcatt ctgcagagga gtcgagtgta tgtaaacttc
     6541 tgacccactg ggaatgtgat gaaagaaata aaagctgaaa tgaatcattc tctctactat
     6601 tattctgata tttcacattc ttaaaataaa gtggtgatcc taactgacct aagacaggga
     6661 atttttacta ggattaaatg tcaggaattg tgaaaaagtg agtttaaatg tatttggcta
     6721 aggtgtatgt aaacttccga cttcaactgt atagggatcc gcttcctcgc tcactgactc
     6781 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     6841 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     6901 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     6961 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     7021 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     7081 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     7141 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     7201 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     7261 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     7321 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     7381 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     7441 ttgatccggc aaacaaacca ccgctggtag cggtggtttt ttt
//
