LOCUS       AiP14974                5270 bp    DNA     circular UNA 14-OCT-2024
DEFINITION  pAAV-AiE1045m-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA.
ACCESSION   urn.local.aaron.oster.5d9-it6u6je
VERSION     urn.local.aaron.oster.5d9-it6u6je
KEYWORDS    .
SOURCE       (pAAV-TelN-LITR-eHGT_1045m-SYFP2-P2A-3XFLAG-10aa-H2B*-WPRE3-BGHpA-RITR-TelN)
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             complement(1..130)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="92.20%"
                     /Primary_Match="92.20%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     Enhancer        144..588
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="AiE1045m"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:111b-hkqxplz"">AiE1045m</a
                     >"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AiE1045m"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:111b-hkqxplz"">AiE1045m</a
                     >"
                     /label="AiE1045m"
     regulatory      623..632
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="Kozak sequence"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kozak sequence"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /label="Kozak sequence"
     CDS             629..1348
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SYFP2"
                     /Transferred_Similarity="98.75%"
                     /Primary_Match="98.75%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cz-gl1nwdj"">SYFP2</a>"
                     /Alternative_Match="97.35%; Adjusted-Interval-0:0/-3; <a
                     href=""urn:local:yoav.ben-simon:14v-gl1nvql"">Cerulean</a>
                     "
                     /Alternative_Match="96.94%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1er-gl1nwjw"">EYFP</a>"
                     /Alternative_Match="96.81%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1aq-gl1nw6h"">mCitrine</a>
                     "
                     /Alternative_Match="96.54%; Adjusted-Interval-0:-3/0; <a
                     href=""urn:local:yoav.ben-simon:163-gl1nvtx"">EGFP</a>"
                     /Alternative_Match="95.56%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1ao-gl1nw6h"">mCerulean3</
                     a>"
                     /label="SYFP2"
     Tag             1424..1489
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="3X FLAG"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13k-gl1nvn3"">3X FLAG</a>"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="3XFLAG"
                     /Transferred_Similarity="98.48%"
                     /Primary_Match="98.48%; Adjusted-Interval-0:0/0; 3X FLAG
                     (<a
                     href=""urn:local:yoav.ben-simon:13k-gl1nvn3"">3XFLAG</a>)"
                     /label="3X FLAG"
     Linker          1490..1519
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="10 a.a. linker"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13i-gl1nvn3"">10 a.a.
                     linker</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="10 a.a. linker"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13i-gl1nvn3"">10 a.a.
                     linker</a>"
                     /label="10 a.a. linker"
     sig_peptide     1520..1894
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="H2B"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:178-gl1nvx6"">H2B</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="H2B"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:178-gl1nvx6"">H2B</a>"
                     /label="H2B"
     3'UTR           1929..2174
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="WPRE3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1e2-gl1nwgr"">WPRE3</a>"
                     /label="WPRE3"
     polyA_signal    2188..2391
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BGH-pA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14r-gl1nvql"">BGH-pA</a>"
                     /label="BGH-pA"
     LTR             2420..2560
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     misc_feature    2567..2622
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TelN_seq"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1n1x-gn0w5a7"">TelN_seq</a
                     >"
                     /label="TelN_seq"
     misc_feature    complement(2567..2622)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TelN_seq"
                     /Transferred_Similarity="92.86%"
                     /Primary_Match="92.86%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1n1x-gn0w5a7"">TelN_seq</a
                     >"
                     /label="TelN_seq"
     rep_origin      2708..3014
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="f1 Origin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="f1 Origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /label="f1 Origin"
     promoter        3428..3532
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vx7-gmqsxiz"">AmpR
                     promoter</a>"
                     /label="AmpR promoter"
     CDS             3533..4393
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Adjusted_Transferred_Similarity="99.65%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/-3
                     (adjusted to 99.65%; Adjusted-Interval-0:0/0); <a
                     href=""urn:local:yoav.ben-simon:13p-gl1nvn3"">AmpR</a>"
                     /label="AmpR"
     rep_origin      4541..5208
                     /vntifkey=33
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori
                     (<a href=""urn:local:yoav.ben-simon:vxh-gmqt1zl"">pUC
                     ori</a>)"
                     /label="pUC\ori"
     misc_feature    5209..5264
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TelN_seq"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1n1x-gn0w5a7"">TelN_seq</a
                     >"
                     /label="TelN_seq"
     misc_feature    complement(5209..5264)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.7 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="TelN_seq"
                     /Transferred_Similarity="92.86%"
                     /Primary_Match="92.86%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1n1x-gn0w5a7"">TelN_seq</a
                     >"
                     /label="TelN_seq"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgttcaatg cctttgttcc ggagtctgtt agagaagaaa
      181 aagttacagt agcataacaa agcaggaaaa gggaatggaa aaaaaaaata ataactggct
      241 tccagtgtct ggagcagctg cacacacacc cttagcacag gtttggcttg gtaaattaat
      301 tcatctgtaa atagtgagca gtctccagat agaacttcag ggctgaggtg caggttcctt
      361 aaggatttac atgtaaaggc aaaggctttc agccctaggt gttacaagtg agtggtgccc
      421 ttcatgccct gccctgagtc tgcccatcca atcatatccc gatatgcaaa tgacccttag
      481 tgcatgcaga taaaaaggag caagggctct ttcagaacca atttgctgag cctgtcacct
      541 gtgttccagg agccaaacca gcaatgtctt cgcctgcaca acctgcaggg atccagatct
      601 ttcgaagcta gcgctaccgg tcgccaccat ggtctccaag ggcgaggagc tgttcaccgg
      661 ggtggtgccc atcctggtcg agctggacgg cgatgtcaac ggccacaagt tcagcgtgtc
      721 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagctga tctgcaccac
      781 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgggctacg gcgtgcagtg
      841 cttcgcccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
      901 aggctacgtc caggagcgca ccatcttctt caaagacgac ggcaactaca agacccgcgc
      961 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     1021 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     1081 ctatatcacc gccgacaagc agaagaacgg catcaaggcc aacttcaaga tccgccacaa
     1141 catcgaggac ggcggcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     1201 cggccccgtg ctgctgcccg acaaccacta cctgagctac cagtccaagc tgagcaaaga
     1261 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     1321 tctcggcatg gacgagctgt acaagggctc tggtgctacc aacttctcac tgttgaaaca
     1381 ggcaggggat gtagaggaga atccagggcc tggtgctagt ggagactaca aagaccatga
     1441 cggagattat aaagatcatg acatcgatta caaggatgac gatgacaagt ccggactcag
     1501 atctggaggc tccggaggcc cagagccagc gaagtctgct cccgccccga aaaagggctc
     1561 caagaaggcg gtgactaagg cgcagaagaa aggcggcaag aagcgcaagc gcagccgcaa
     1621 ggagagctat tccatctatg tgtataaggt tctgaagcag gtccaccctg acaccggcat
     1681 ttcgtccaag gccatgggca tcatgaactc gtttgtgaac gacattttcg agcgcatcgc
     1741 aggtgaggct tcccgcctgg cgcattacaa caagcgctcg accatcacct ccagggagat
     1801 ccagacggcc gtgcgcctgc tgctgcctgg ggagttggcc aagcacgccg tgtccgaggg
     1861 tactaaggcc atcaccaagt acaccagcgc taagtaatga ggcgcgccgc ggccgcgaat
     1921 tcgatatcat aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     1981 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     2041 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag ttcttgccac
     2101 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
     2161 tgacaattcc gtggctcgag agatcttcga ctgtgccttc tagttgccag ccatctgttg
     2221 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct
     2281 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg
     2341 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gagatctcac
     2401 gtgcggaccg agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc
     2461 gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg
     2521 gcggcctcag tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctatc agcacacaat
     2581 tgcccattat acgcgcgtat aatggactat tgtgtgctga tatgatgcgg tattttctcc
     2641 ttacgcatct gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg
     2701 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     2761 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     2821 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     2881 gcacctcgac cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg
     2941 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     3001 ccaaactgga acaacactca accctatctc gggctattct tttgatttat aagggatttt
     3061 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     3121 taacaaaata ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc
     3181 cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg
     3241 tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca
     3301 gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt
     3361 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
     3421 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     3481 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     3541 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     3601 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     3661 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     3721 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     3781 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     3841 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     3901 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     3961 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4021 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4081 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     4141 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     4201 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     4261 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     4321 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     4381 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     4441 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     4501 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     4561 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     4621 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     4681 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
     4741 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     4801 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
     4861 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
     4921 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
     4981 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
     5041 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
     5101 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
     5161 caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcata tcagcacaca
     5221 attgcccatt atacgcgcgt ataatggact attgtgtgct gataacatgt
//
