LOCUS       AiP2126                 4788 bp    DNA     circular UNA 01-MAY-2024
DEFINITION  pAAV-AiE2652m-minBG-SYFP2-WPRE-BGHpA.
ACCESSION   Circular
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             complement(1..130)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="92.20%"
                     /Primary_Match="92.20%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     Enhancer        145..698
                     /X-Transferred_From="AiE2652m"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14nv-hkqyii4"">AiE2652m</a
                     >"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AiE2652m"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14nv-hkqyii4"">AiE2652m</a
                     >"
                     /label="AiE2652m"
     promoter        713..765
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Beta Globin minimal promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; minBG
                     promoter (<a
                     href=""urn:local:yoav.ben-simon:14q-gl1nvql"">Beta Globin
                     minimal promoter</a>)"
                     /label="minBG promoter"
     regulatory      800..809
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="Kozak sequence"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kozak sequence"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /label="Kozak sequence"
     CDS             806..1525
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SYFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cz-gl1nwdj"">SYFP2</a>"
                     /label="SYFP2"
     3'UTR           1560..1805
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="WPRE3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1e2-gl1nwgr"">WPRE3</a>"
                     /label="WPRE3"
     polyA_signal    1819..2022
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BGH-pA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14r-gl1nvql"">BGH-pA</a>"
                     /label="BGH-pA"
     LTR             2051..2191
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     rep_origin      2283..2589
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="f1 Origin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="f1 Origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /label="f1 Origin"
     promoter        3003..3107
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vx7-gmqsxiz"">AmpR
                     promoter</a>"
                     /label="AmpR promoter"
     misc_feature    3038..3045
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:174-gl1nvx6"">FRT3 spacer
                     (FASEBJ 2011, 25, 1)</a>"
                     /label="FRT3 spacer (FASEBJ 2011, 25, 1)"
     CDS             3108..3965
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13p-gl1nvn3"">AmpR</a>"
                     /label="AmpR"
     rep_origin      4116..4783
                     /vntifkey=33
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori
                     (<a href=""urn:local:yoav.ben-simon:vxh-gmqt1zl"">pUC
                     ori</a>)"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgaaaat actaaacaga aaaaaaaaca tgaaactgta
      181 ctgcatacta tattattcat tcacatactg ttgagtttag gggactagaa tgggctggaa
      241 agacagaatc tgctatgcgc cagggaggtt ccctgtaggg aaagagaagg tgtcagtcct
      301 gaatttgttt gctctgggac ctggtcccca ctcagcccat gcttggctca gacctggtct
      361 caggcccctg ttttgacaag attcctttct aaacagcttc ctacagtgtt cctaacaaga
      421 gtgacatttg cagatgctta tagagaattg agaaagcaaa ccccaggatt cctccatccc
      481 tattttgggc ctcagtttaa accctcgccc caaccaaccc ttccctcctc caagtccctc
      541 cttcaagcta accctgctcc agattctgac tacctaatca ccctgtcatt atcttctagg
      601 ctcctttagc ccctcccccc agagttttca accctctctg tcttcagagt agtctctcat
      661 ttcctattgg atcctgatgc aatggagaaa taagtgttcg ccggcggagc tcgggctggg
      721 cataaaagtc agggcagagc catctattgc ttacatttgc ttctgggatc cagatctttc
      781 gaagctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt
      841 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
      901 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct gcaccaccgg
      961 caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcg tgcagtgctt
     1021 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     1081 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     1141 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     1201 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     1261 tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     1321 cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     1381 ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga gcaaagaccc
     1441 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     1501 cggcatggac gagctgtaca agtaagtcga cggcgcgccg cggccgcgaa ttcgatatca
     1561 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     1621 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     1681 tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact
     1741 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     1801 cgtggctcga gagatcttcg actgtgcctt ctagttgcca gccatctgtt gtttgcccct
     1861 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg
     1921 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc
     1981 aggacagcaa gggggaggat tgggaagaca atagcaggca tgagatctca cgtgcggacc
     2041 gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg cgctcgctcg
     2101 ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg ggcggcctca
     2161 gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt tctccttacg
     2221 catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg ccctgtagcg
     2281 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     2341 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     2401 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     2461 tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg ccctgataga
     2521 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     2581 ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg attttgccga
     2641 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     2701 aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct gatgccgcat
     2761 agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
     2821 tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg tgtcagaggt
     2881 tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc ctatttttat
     2941 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     3001 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     3061 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     3121 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     3181 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     3241 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     3301 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     3361 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     3421 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     3481 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3541 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3601 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3661 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3721 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3781 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3841 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3901 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     3961 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     4021 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     4081 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     4141 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     4201 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     4261 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca
     4321 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     4381 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     4441 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     4501 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4561 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4621 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4681 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4741 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
