LOCUS       AiP2129                 4858 bp    DNA     circular UNA 01-MAY-2024
DEFINITION  pAAV-AiE2656m-minBG-SYFP2-WPRE-BGHpA.
ACCESSION   Circular
KEYWORDS    .
SOURCE      
  ORGANISM  .
FEATURES             Location/Qualifiers
     LTR             complement(1..130)
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="92.20%"
                     /Primary_Match="92.20%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     Enhancer        145..768
                     /X-Transferred_From="AiE2656m"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14nz-hkqyii4"">AiE2656m</a
                     >"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AiE2656m"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14nz-hkqyii4"">AiE2656m</a
                     >"
                     /label="AiE2656m"
     promoter        783..835
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Beta Globin minimal promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; minBG
                     promoter (<a
                     href=""urn:local:yoav.ben-simon:14q-gl1nvql"">Beta Globin
                     minimal promoter</a>)"
                     /label="minBG promoter"
     regulatory      870..879
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="Kozak sequence"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="Kozak sequence"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:19j-gl1nw38"">Kozak
                     sequence</a>"
                     /label="Kozak sequence"
     CDS             876..1595
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="SYFP2"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cz-gl1nwdj"">SYFP2</a>"
                     /label="SYFP2"
     3'UTR           1630..1875
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="WPRE3"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1e2-gl1nwgr"">WPRE3</a>"
                     /label="WPRE3"
     polyA_signal    1889..2092
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="BGH-pA"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:14r-gl1nvql"">BGH-pA</a>"
                     /label="BGH-pA"
     LTR             2121..2261
                     /created_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="ITR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:1cu-gl1nwdj"">ITR</a>"
                     /label="ITR"
     rep_origin      2353..2659
                     /note="Derived using Geneious Prime 2023.2.1 'Annotate
                     from Database' based on nucleotide similarity"
                     /X-Transferred_From="f1 Origin"
                     /X-Transferred_Similarity="100.00%"
                     /X-Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="f1 Origin"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:170-gl1nvx6"">f1
                     Origin</a>"
                     /label="f1 Origin"
     promoter        3073..3177
                     /gene="bla"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR promoter"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:vx7-gmqsxiz"">AmpR
                     promoter</a>"
                     /label="AmpR promoter"
     misc_feature    3108..3115
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="FRT3 spacer (FASEBJ 2011, 25, 1)"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:174-gl1nvx6"">FRT3 spacer
                     (FASEBJ 2011, 25, 1)</a>"
                     /label="FRT3 spacer (FASEBJ 2011, 25, 1)"
     CDS             3178..4035
                     /created_by="yoav.ben-simon"
                     /modified_by="yoav.ben-simon"
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="AmpR"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; <a
                     href=""urn:local:yoav.ben-simon:13p-gl1nvn3"">AmpR</a>"
                     /label="AmpR"
     rep_origin      4186..4853
                     /vntifkey=33
                     /note="Derived using Geneious Prime 2024.0.4 'Annotate
                     from Database' based on nucleotide similarity"
                     /Transferred_From="pUC ori"
                     /Transferred_Similarity="100.00%"
                     /Primary_Match="100.00%; Adjusted-Interval-0:0/0; pUC\ori
                     (<a href=""urn:local:yoav.ben-simon:vxh-gmqt1zl"">pUC
                     ori</a>)"
                     /label="pUC\ori"
ORIGIN      
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtattgaa agccatcacc ctaaatgagg tcatcaggat
      181 tagagaaaag agtatccagc actatgctac agataaagaa aggccaaggg gagagggaaa
      241 ctcaaggaca ctgagaagtc ataggagata gaaatgcttt gtccattgac atgggaaaac
      301 aaagcattta aaaatgagaa ttgctgggcc tttcggaatc ccaggacttt caccttaagg
      361 taaggtgaat aagagcatct ctgctggggt gtttatgagg atgacatctg taatatatgt
      421 aaatgttggc attgtgtctc atgatgggca ctcgatcaat gtctgttatc ctcctgttgg
      481 ttgtgttacc agacccttgg ggaaagagaa ccttccagac aaccttctta tattcttgca
      541 gtctgtgttg actcaatatg gtaaatagct acttgtctga gaaaatattt atgcaggatg
      601 aataggacaa atattgattt tgttctaccc tggcatccat ataactcaag gacaagggag
      661 actccgcaca tgaattttag aaacagtaac tgtgctactc actctctcaa ccctgtctta
      721 gtaattaatg tgttaacact tgtgctgtga acaaatgaat aaaggactcg ccggcggagc
      781 tcgggctggg cataaaagtc agggcagagc catctattgc ttacatttgc ttctgggatc
      841 cagatctttc gaagctagcg ctaccggtcg ccaccatggt gagcaagggc gaggagctgt
      901 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      961 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagctgatct
     1021 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg ggctacggcg
     1081 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     1141 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     1201 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     1261 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     1321 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
     1381 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
     1441 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagctaccag tccaagctga
     1501 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1561 ggatcactct cggcatggac gagctgtaca agtaagtcga cggcgcgccg cggccgcgaa
     1621 ttcgatatca taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     1681 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     1741 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggtta gttcttgcca
     1801 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     1861 ctgacaattc cgtggctcga gagatcttcg actgtgcctt ctagttgcca gccatctgtt
     1921 gtttgcccct cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc
     1981 taataaaatg aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt
     2041 ggggtggggc aggacagcaa gggggaggat tgggaagaca atagcaggca tgagatctca
     2101 cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc cctctctgcg
     2161 cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg gctttgcccg
     2221 ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga tgcggtattt
     2281 tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc atagtacgcg
     2341 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     2401 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     2461 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     2521 ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag tgggccatcg
     2581 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     2641 ttgttccaaa ctggaacaac actcaaccct atctcgggct attcttttga tttataaggg
     2701 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     2761 aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac aatctgctct
     2821 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     2881 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     2941 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     3001 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     3061 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     3121 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     3181 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     3241 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     3301 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     3361 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     3421 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     3481 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     3541 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     3601 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     3661 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     3721 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     3781 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     3841 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     3901 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     3961 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     4021 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     4081 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     4141 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     4201 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     4261 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     4321 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     4381 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     4441 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     4501 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     4561 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     4621 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     4681 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     4741 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     4801 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgt
//
